Histological and hematological changes to Clarias gariepinus juveniles exposed to acute doses of Emamectin benzoate in a static bioassay.

Emamectin benzoate (EMB) is a potent neurotoxic pesticide, extensively used in agriculture and aquaculture in Nigeria. The knowledge of its toxicological impact to C. gariepinus in Nigeria is scarce. Thus, the investigation was designed to uncover its 96 h LC50, safe level to aquatic media, histological impact on fish liver, gill and hematological changes in the blood parameters. 96 h LC50 was 0.34 mg L-1. Safe level of EMB was 0.034 mg L-1. Dose dependent liver degenerations were characterized by; Congestion of the central vein by inflammatory cells, pyknotic nuclei of hepatocytes, coagulation necrosis, focal necrosis, dilations of sinusoidal spaces and infiltration of periportal regions by inflammatory cells. Dose dependent changes in gill were characterized by; mucus secretion, shrinkage of secondary lamellae, hyperplasia, occlusion of secondary lamellae, degeneration of gill cartilage, necrosis of respiratory epithelia and erosion of secondary lamellae. Red blood cell indices decreased minimally at the end of the 96 h exposure. White blood cell count (WBCC), mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were significantly (p ≤ 0.05) elevated in three treatments. Neutrophils decreased significantly (p ≤ 0.05), basophils, eosinophils and monocytes showed mixed tendencies. The findings of this investigation imply that C. garipinus exposed to EMB may cause dose and time-dependent changes in the liver and gill histology as well as alterations in the fish's hematological profile, all of which were harmful to the fish's health. To avoid negative effects on fish in nearby aquatic settings, it is advised that the use of EMB be monitored and limited.

Presentation weight indices for patients with cleft and non-affected children: A two-centre study.

Background: Children with cleft lip and/or palate (CL/P) face many challenges in the society. They tend to suffer malnutrition and therefore poor weight gain due to lack of standard care, especially from their parents and society at large. The poor weight gain leads to delayed surgical repair of the cleft abnormality leading to prolonged nutritional and psychological challenges for all concerned. Objective: The aim of the study was to determine if children without cleft abnormalities presenting for routine immunisation and children with cleft abnormalities are both likely to be less than the 75th percentile of expected weight at the time of presentation, and if there is any difference in presenting weight amongst children with different cleft types. Materials and Methods: This was a retrospective study. Data of children that attended the immunisation clinic of the Department of Primary Health of the National Orthopaedic Hospital, Enugu, and routine surgical clinic of the National Orthopaedic Hospital, Enugu, Nigeria, and the Good Shepherd Specialist Hospital, Enugu, Nigeria, between January 2010 and December 2014 with a diagnosis of CL/P, were obtained from the medical records. The data were analysed with SPSS and the confidence interval was 95%. Results: A total of 923 medical records were reviewed for the study, out of which 363 were for children with cleft abnormalities. There was no significant difference in the weight of the children without cleft and presenting for routine immunisation compared with their expected 75th percentile, or children with cleft abnormality compared with their expected 75th percentile (either in their first or in their second visits). With the children that presented with CL/P, there was a significant difference noted amongst children with both CL/P deformity with the expected 75th percentile weight, irrespective of whether the combination was unilateral or bilateral, with P = 0.041. This difference was noted in their first visit. Conclusion: Children with combined CL/P are at risk of presenting with poor weight. Nutritional rehabilitation considered early with children with cleft and particularly those children with combined CL/P.