Pesquisa | Biblioteca Virtual em Saúde

Understanding relative survival outcomes for patients with cutaneous T-cell lymphoma (CTCL) subtypes mycosis fungoides and Sézary syndrome treated with mogamulizumab or vorinostat: combining Australian real-world evidence and MAVORIC phase 3 trial data.

Remák, Edit; Hawkins, Neil; Jones, Trefor; Otley, Marissa; Twigger, Robert; Prince, Miles.

Eur J Cancer ; 156 Suppl 1: S18, 2021 Oct.

Artigo em Inglês | MEDLINE | ID: mdl-34649645

Overexpression of cardiac actin with baculovirus is promoter dependent.

Yates, Susan P; Otley, Marissa D; Dawson, John F.

Arch Biochem Biophys ; 466(1): 58-65, 2007 Oct 01.

Artigo em Inglês | MEDLINE | ID: mdl-17765196

RESUMO

The influence of the promoter and an N-terminal hexahistidine tag on human cardiac actin (ACTC) expression and function was investigated using four baculovirus constructs. It was found that both non-tagged ACTC and hisACTC expression from the p10 promoter was higher than from the polh promoter. Characterization showed that an N-terminal hexahistidine tag has a negative effect on ACTC. Recombinant ACTC inhibits DNase-I and binds myosin S1, indicative of proper folding. Our data support the hypothesis that the actin protein down-regulates the polh promoter.

Assuntos

Actinas/genética , Baculoviridae/genética , Vetores Genéticos/genética , Miocárdio/metabolismo , Regiões Promotoras Genéticas/genética , Engenharia de Proteínas/métodos , Proteínas Recombinantes/metabolismo , Spodoptera/genética , Spodoptera/metabolismo , Transfecção/métodos , Animais , Linhagem Celular , Spodoptera/virologia

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