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1.
Opt Express ; 30(21): 37457-37469, 2022 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-36258334

RESUMO

It is always a challenge how to overcome speckle noise interference in the phase reconstruction for coherent digital holography (CDH) and its application, as this issue has not been solved well so far. In this paper, we are proposing an enhanced anti-speckle deep neural unwrapping network (E-ASDNUN) approach to achieve high quality of absolute phase reconstruction for CDH. The method designs a special network-based noise filter and embeds it into a deep neural unwrapping network to enhance anti-noise capacity in the image feature recognition and extraction process. The numerical simulation and experimental test on the phase unwrapping reconstruction and the image quality evaluation under the noise circumstances show that the E-ASDNUN approach is very effective against the speckle noise in realizing the high quality of absolute phase reconstruction. Meanwhile, it also demonstrates much better robustness than the typical U-net neural network and the traditional phase unwrapping algorithms in reconstructing high wrapping densities and high noise levels of phase images. The E-ASDNUN approach is also examined and confirmed by measuring the same phase object using a commercial white light interferometry as a reference. The result is perfectly consistent with that obtained by the E-ASDNUN approach.


Assuntos
Holografia , Holografia/métodos , Algoritmos , Interferometria/métodos , Simulação por Computador , Redes Neurais de Computação
2.
Sensors (Basel) ; 20(22)2020 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-33212842

RESUMO

Digital gradient sensing (DGS) is a non-contact and full-field optical measurement technique, which assesses mechanical behaviors of transparent materials or specular structures by measuring angular deflections of light rays. However, owing to the poor light-gathering capability of its imaging system, the dynamic performance of DGS is heavily restricted. Here, a method of enhancing the dynamic performance of DGS by improving its speckle target is proposed. The method employs the technique of backlight illumination, which significantly increases the utilization efficiency of light, shortens the exposure time, and enhances the dynamic performance of DGS. Additionally, it also uses the optimized digital speckle pattern to improve the measurement precision and accuracy. For validation, a comparison experiment was conducted, proving that the proposed method can improve the utilization efficiency of light by about 80 times and improve the quality of the speckle images by about 40%. Real tests, including a uniaxial tension test using transmission-mode DGS (t-DGS) and a three-point bending test using reflection-mode DGS (r-DGS), were also carried out, showing the efficacy and high compatibility of the proposed backlight digital speckle target. In summary, this simple method greatly improves the performance of DGS, which can be used as a standard method in both t-DGS and r-DGS.

3.
Angew Chem Int Ed Engl ; 58(8): 2261-2265, 2019 02 18.
Artigo em Inglês | MEDLINE | ID: mdl-30589211

RESUMO

Enzyme activity in live cells is dynamically regulated by small-molecule transmitters for maintaining normal physiological functions. A few probes have been devised to measure intracellular enzyme activities by fluorescent imaging, but the study of the regulation of enzyme activity via gasotransmitters in situ remains a long-standing challenge. Herein, we report a three-channel imaging correlation by a single dual-reactive fluorescent probe to measure the dependence of phosphatase activity on the H2 S level in cells. The two sites of the probe reactive to H2 S and phosphatase individually produce blue and green fluorescent responses, respectively, and resonance energy transfer can be triggered by their coexistence. Fluorescent analysis based on the three-channel imaging correlation shows that cells have an ideal level of H2 S to promote phosphatase activity up to its maximum. Significantly, a slight deviation from this H2 S level leads to a sharp decrease of phosphatase activity. The discovery further strengthens our understanding of the importance of H2 S in cellular signaling and in various human diseases.


Assuntos
Corantes Fluorescentes/metabolismo , Gasotransmissores/metabolismo , Imagem Óptica , Monoéster Fosfórico Hidrolases/metabolismo , Corantes Fluorescentes/química , Gasotransmissores/química , Células HeLa , Humanos , Sulfeto de Hidrogênio/metabolismo , Estrutura Molecular
4.
Opt Express ; 21(14): 16736-41, 2013 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-23938525

RESUMO

The majority of existing real-time 3D shape measurement systems only generate non-nature texture (i.e., having illumination other than ambient lights) that induces shadow related issues. This paper presents a method that can simultaneously capture natural 2D color texture and 3D shape in real time. Specifically, we use an infrared fringe projection system to acquire 3D shapes, and a secondary color camera to simultaneously capture 2D color images of the object. Finally, we develop a flexible and simple calibration technique to determine the mapping between the 2D color image and the 3D geometry. Experimental results demonstrate the success of the proposed technique.


Assuntos
Algoritmos , Colorimetria/instrumentação , Colorimetria/métodos , Imageamento Tridimensional/instrumentação , Imageamento Tridimensional/métodos , Refratometria/instrumentação , Refratometria/métodos , Sistemas Computacionais , Desenho de Equipamento , Análise de Falha de Equipamento
5.
Appl Opt ; 52(9): 1857-63, 2013 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-23518729

RESUMO

Prior studies on converting three-dimensional (3D) range data into regular two-dimensional (2D) color images using virtual fringe projection techniques showed great promise for 3D range data compression, yet they require resampling the raw scanned data. Due to this resampling, the natural 3D range data are altered and sampling error may be introduced. This paper presents a method that compresses the raw sampling points without modifications. Instead of directly utilizing the 3D recovered shape, this method compresses the s map, the scale factor of a perspective projection from a 3D space to a 2D space. The s map is then converted to 2D color image for further compression with existing 2D image compression techniques. By this means, 3D data obtained by 3D range scanners can be compressed into 2D images without any resampling, providing a natural and more accurate method of compressing 3D range data. Experimental results verified the success of the proposed method.

6.
Opt Express ; 16(7): 5130-5, 2008 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-18542612

RESUMO

In this paper a novel optical generation approach for binary-phase-coded, direct-sequence ultra-wideband (UWB) signals is experimentally demonstrated. Our system consists of a laser array, a polarization modulator (PolM), a fiber Bragg grating (FBG), a length of single mode fiber, and a photo detector (PD). The FBG, designed based on the superimposed, chirped grating, is used as the multi-channel frequency discriminator. The input electronic Gaussian pulse is modulated on the optical carrier by the PolM and then converted into UWB monocycle or doublet pulses sequence by the multi-channel frequency discriminator. The PolM is used so that the desired binary phase code pattern could be simply selected by adjusting the polarization state of each laser, rather than tuning the laser wavelengths. The desired UWB shape, monocycle or doublet, could be selected by tuning the FBG. Based on our proposed approach, four-chip, binary-phase-coded, DS-UWB sequences with different pulse shapes and code patterns are experimentally demonstrated. The impact of the fiber dispersion on the generated UWB pulses is also discussed in our paper.


Assuntos
Tecnologia de Fibra Óptica/instrumentação , Lasers , Fotometria/instrumentação , Refratometria/instrumentação , Processamento de Sinais Assistido por Computador/instrumentação , Telecomunicações/instrumentação , Desenho de Equipamento , Análise de Falha de Equipamento
7.
Opt Express ; 16(25): 20968-73, 2008 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-19065236

RESUMO

This study explores two-dimensional binary sub-wavelength diffractive lenses (BSDLs) for implementing long focal depth and high transverse resolution based on the rigorous electromagnetic theory and the finite-difference time-domain method. Focusing performances, such as the actual focal depth, the ratio between the focal depth of the designed BSDL and the focal depth of the conventional sub-wavelength lens and the spot size of the central lobe at the actual focal plane, for different f-numbers, have been studied in the case of TE incidence polarization wave. The rigorous numerical results indicate that the designed BSDLs indeed have long focal depth and high transverse resolution by modulating the binary sub-wavelength characteristic sizes. Because BSDLs have the ability for monolithic integration and can require only single step fabrication, the investigations may provide useful information for BSDLs' application in micro-optical systems.


Assuntos
Desenho Assistido por Computador , Lentes , Modelos Teóricos , Refratometria/instrumentação , Simulação por Computador , Desenho de Equipamento , Análise de Falha de Equipamento , Luz , Espalhamento de Radiação
8.
Opt Express ; 13(7): 2699-706, 2005 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-19495161

RESUMO

A new type of monolithic high-power fiber laser with a multifiber series and distributed pumping is put forward. The distributed pumping multifiber series fiber laser is a good solution to scale up laser output when injection power is limited by the output powers of a pigtailed laser diode or the maximum sustainable powers of pumping couplers. The simulation results show that the laser has advantages of high transfer efficiency and good temperature uniformity in the kilowatt power domain.

9.
Opt Express ; 13(14): 5591, 2005 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-19498556

RESUMO

We have an addendum to acknowledge two previous papers. The cooling condition for the proposed fiber laser is clarified.

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