Identifying key genes involved in yellow leaf variation in 'Menghai Huangye' based on biochemical and transcriptomic analysis.

Albino tea plants generally have higher theanine, which causes their tea leaves to taste fresher, and they are an important mutant for the breeding of tea plant varieties. Earlier, we reported an albino germplasm, 'Menghai Huangye' (MHHY), from Yunnan Province and found that it has a lower chlorophyll content during the yellowing stage, but the mechanism underlying low chlorophyll and the yellowing phenotype is still unclear. In this study, the pigment contents of MHHY_May (yellowing, low chlorophyll), MHHY_July (regreening, normal chlorophyll), and YK10_May (green leaves, normal chlorophyll) were determined, and the results showed that the lower chlorophyll content might be an important reason for the formation of the yellowing phenotype of MHHY. Through transcriptome sequencing, we obtained 654 candidates for differentially expressed genes (DEGs), among which 4 genes were related to chlorophyll synthesis, 10 were photosynthesis-related, 34 were HSP family genes, and 19 were transcription factor genes. In addition, we analysed the transcription levels of the key candidate genes in MHHY_May and MHHY_July and found that they are consistent with the expression trends in MHHY_May and YK10_May, which further indicates that the candidate differential genes we identified are likely to be key candidate factors involved in the low chlorophyll content and yellowing of MHHY. In summary, our findings will assist in revealing the low chlorophyll content of MHHY and the formation mechanism of yellowing tea plants and will be applied to the selection and breeding of albino tea cultivars in the future.

Integrated transcriptome and hormonal analysis of naphthalene acetic acid-induced adventitious root formation of tea cuttings (Camellia sinensis).

BACKGROUND: Tea plant breeding or cultivation mainly involves propagation via cuttings, which not only ensures the inheritance of the excellent characteristics of the mother plant but also facilitates mechanized management. The formation of adventitious root (AR) determines the success of cutting-based propagation, and auxin is an essential factor involved in this process. To understand the molecular mechanism underlying AR formation in nodal tea cuttings, transcriptome and endogenous hormone analysis was performed on the stem bases of red (mature)- and green (immature)-stem cuttings of 'Echa 1 hao' tea plant as affected by a pulse treatment with naphthalene acetic acid (NAA). RESULTS: In this study, NAA significantly promoted AR formation in both red- and green-stem cuttings but slightly reduced callus formation. External application of NAA reduced the levels of endogenous indole-3-acetic acid (IAA) and cytokinin (TZR, trans-zeatin riboside). The number of DEGs (NAA vs. CK) identified in the green-stem cuttings was significantly higher than that in the red-stem cuttings, which corresponded to a higher rooting rate of green-stem cuttings under the NAA treatment. A total of 82 common DEGs were identified as being hormone-related and involved in the auxin, cytokinin, abscisic acid, ethylene, salicylic acid, brassinosteroid, and jasmonic acid pathways. The negative regulation of NAA-induced IAA and GH3 genes may explain the decrease of endogenous IAA. NAA reduced endogenous cytokinin levels and further downregulated the expression of cytokinin signalling-related genes. By the use of weighted gene co-expression network analysis (WGCNA), several hub genes, including three [cellulose synthase (CSLD2), SHAVEN3-like 1 (SVL1), SMALL AUXIN UP RNA (SAUR21)] that are highly related to root development in other crops, were identified that might play important roles in AR formation in tea cuttings. CONCLUSIONS: NAA promotes the formation of AR of tea cuttings in coordination with endogenous hormones. The most important endogenous AR inductor, IAA, was reduced in response to NAA. DEGs potentially involved in NAA-mediated AR formation of tea plant stem cuttings were identified via comparative transcriptome analysis. Several hub genes, such as CSLD2, SVL1 and SAUR21, were identified that might play important roles in AR formation in tea cuttings.

The language of character strengths: Predicting morally valued traits on social media.

OBJECTIVE: Social media is increasingly being used to study psychological constructs. This study is the first to use Twitter language to investigate the 24 Values in Action Inventory of Character Strengths, which have been shown to predict important life domains such as well-being. METHOD: We use both a top-down closed-vocabulary (Linguistic Inquiry and Word Count) and a data-driven open-vocabulary (Differential Language Analysis) approach to analyze 3,937,768 tweets from 4,423 participants (64.3% female), who answered a 240-item survey on character strengths. RESULTS: We present the language profiles of (a) a global positivity factor accounting for 36% of the variances in the strengths, and (b) each of the 24 individual strengths, for which we find largely face-valid language associations. Machine learning models trained on language data to predict character strengths reach out-of-sample prediction accuracies comparable to previous work on personality (rmedian = 0.28, ranging from 0.13 to 0.51). CONCLUSIONS: The findings suggest that Twitter can be used to characterize and predict character strengths. This technique could be used to measure the character strengths of large populations unobtrusively and cost-effectively.

Comparative transcriptomic analysis of the tea plant (Camellia sinensis) reveals key genes involved in pistil deletion.

BACKGROUND: The growth process of the tea plant (Camellia sinensis) includes vegetative growth and reproductive growth. The reproductive growth period is relatively long (approximately 1.5 years), during which a large number of nutrients are consumed, resulting in reduced tea yield and quality, accelerated aging, and shortened economic life of the tea plant. The formation of unisexual and sterile flowers can weaken the reproductive growth process of the tea plant. To further clarify the molecular mechanisms of pistil deletion in the tea plant, we investigated the transcriptome profiles in the pistil-deficient tea plant (CRQS), wild tea plant (WT), and cultivated tea plant (CT) by using RNA-Seq. RESULTS: A total of 3683 differentially expressed genes were observed between CRQS and WT flower buds, with 2064 upregulated and 1619 downregulated in the CRQS flower buds. These genes were mainly involved in the regulation of molecular function and biological processes. Ethylene synthesis-related ACC synthase genes were significantly upregulated and ACC oxidase genes were significantly downregulated. Further analysis revealed that one of the WIP transcription factors involved in ethylene synthesis was significantly upregulated. Moreover, AP1 and STK, genes related to flower development, were significantly upregulated and downregulated, respectively. CONCLUSIONS: The transcriptome analysis indicated that the formation of flower buds with pistil deletion is a complex biological process. Our study identified ethylene synthesis, transcription factor WIP, and A and D-class genes, which warrant further investigation to understand the cause of pistil deletion in flower bud formation.

Different Modulatory Effects of IL-17, IL-22, and IL-23 on Osteoblast Differentiation.

OBJECTIVES: To examine the expressions of IL-17, IL-22, and IL-23 receptors in four osteoblast models and the effects of IL-17, IL-22, and IL-23 on osteoblasts. METHODS: Gene expression levels of receptors, alkaline phosphatase (ALP), osteocalcin (OCN), and Runt-related transcription factor 2 (Runx-2), were evaluated by RT-PCR and real-time RT-PCR. Proliferative responses and cell cycle analysis were detected by a CCK-8 assay and flow cytometry, respectively. ALP activity and ALP mass were detected by an ALP activity assay and ALP staining, respectively. RESULTS: In primary osteoblasts, only the IL-17 receptor was expressed. In C2C12, MC3T3-E1, and Saos-2 cells, the genes of IL-17, IL-22, and IL-23 receptors were not detectable. None of IL-17, IL-22, and IL-23 had an obvious effect on the proliferation of primary osteoblasts, but IL-17 exhibited an inhibitory effect on the gene expression of ALP, OCN, and Runx-2. The ALP activity and ALP mass of primary osteoblasts were downregulated by IL-17 treatment in a dose-dependent manner, and IL-17 failed to inhibit BMP-2-induced phosphorylation of Smad. CONCLUSION: Primary osteoblasts constitutively express IL-17 receptors, but none of C2C12 cells, MC3T3-E1 cells, and Saos-2 cells express any receptors for IL-17, IL-22, and IL-23. IL-17 inhibits BMP-2-induced osteoblast differentiation via the BMP/Smad-independent pathway.

Photocatalytic decomposition of acrylonitrile with N-F codoped TiO2/SiO2 under simulant solar light irradiation.

The solid acid catalyst, N-F codoped TiO2/SiO2 composite oxide was prepared by a sol-gel method using NH4F as nitrogen and fluorine source. The prepared materials were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), UV-Visible diffuse reflectance spectroscopy (UV-Vis), ammonia adsorption and temperature-programmed desorption (NH3-TPD), in situ Fourier transform infrared spectroscopy (FT-IR) and N2 physical adsorption isotherm. The photocatalytic activity of the catalyst for acrylonitrile degradation was investigated under simulant solar irradiation. The results showed that strong Lewis and Brønsted acid sites appear on the surface of the sample after N-F doping. Systematic investigation showed that the highest photocatalytic activity for acrylonitrile degradation was obtained for samples calcined at 450°C with molar ratio (NH4F to Ti) of 0.8. The degradation ratio of 71.5% was achieved with the prepared catalyst after 6-min irradiation, demonstrating the effectiveness of photocatalytic degradation of acrylonitrile with N-F codoped TiO2/SiO2 composite oxide. The photocatalyst is promising for application under solar light irradiation. Moreover, the intermediates generated after irradiation were verified by gas chromatography-mass spectrometry (GC-MS) analysis and UV-Vis spectroscopy to be simple organic acids with lower toxicity, and the degradation pathway was also proposed for acrylonitrile degradation with the prepared catalyst.

A new ligustrazine derivative--pharmacokinetic evaluation and antitumor activity by suppression of NF-kappaB/p65 and COX-2 expression in S180 mice.

A new anticancer ligustrazine derivative, 3beta-hydroxyolea-12-en-28-oic acid-3,5,6-trimethylpyrazin-2-methyl ester (T-OA, C38H58O3N2), was previously reported. It was synthesized via conjugating the effective antitumor ingredients of a classic traditional Chinese medicine (TCM) formulation. In the present study, anticancer efficacy of T-OA was evaluated in vivo using a murine sarcoma S180 model. Reduction of the tumor weight and tumor HE staining regions demonstrated that T-OA had promising inhibition effects and a 50% inhibitory rate in S180 mice. Combining the immunohistochemistry, we found T-OA exerted its antitumor activity by preventing the expression of nuclear transcription factor NF-kappaB/p65 and COX-2 in S180 mice. The acute toxic test showed that LD50 value of T-OA exceeded 6.0 g/kg via gavage in mice. In addition, a simple and rapid HPLC-UV method was developed and validated to study the pharmacokinetic characteristics of the compound. After single-dose oral administration, time to reach peak concentration of T-OA (3.97 microg/mL) was 8.33 h; the elimination half-life and area under the concentration-time curve from t = 0 to the last time of T-OA was 4.50 h and 48.01 microg x h/mL, respectively.

Menghai Huangye, a novel albino tea germplasm with high theanine content and a high catechin index.

Yunnan Province has a very wide diversity of tea germplasm resources. A variety of special tea germplasms with outstanding traits have been discovered, including tea germplasms with high anthocyanin content and low caffeine content. Albino tea cultivars generally have higher contents of theanine that contribute to the umami taste, and the quality of tea brewed from it is higher. The catechin index (CI), the ratio of dihydroxylated catechins (DIC) to trihydroxylated catechins (TRIC), is a crucial index of suitability for processing tea. In this study, the albino tea plant Menghai Huangye (MHHY) with yellow leaves was identified. Analysis of the biochemical components revealed that MHHY was enriched in theanine and the total catechins (TC) were lower than Yunkang 10 (YK10). In addition, the CI value of MHHY was extremely significantly higher than that of YK10. Metabolic profile of catechins and the related gene expression profile analysis found that the coordinated expression of the key branch genes F3'H and F3'5'Ha for the synthesis of DIC and TRIC in tea plant was closely related to the high CI and low TC of MHHY. Further analysis of the F3'H promoter showed that a 284-bp deletion mutation was present in the F3'H promoter of MHHY, containing the binding sites of the transcriptional repressor MYB4 involved in flavonoid metabolism, which might be an important reason for the up-regulated expression of F3'H in MHHY. Overall, this study provides a theoretical basis for understanding the characteristics of albino tea germplasm resources and efficiently utilizing high-CI tea germplasm resources.

Efficacy on rabbits with arrhythmia: needling acupoint of Neiguan (PC6) at shallow or deep depth, and retaining needles for 10, 20, or 30 min.

OBJECTIVE: To compare the effects of Neiguan (PC6) acupuncture at different depths and retention time on arrhythmia duration, myocardial tissue morphology, mRNA expression level of L-type calcium channel α1C subunit and Ca2 + -Mg2 + -AtPase activity in tachirrhythmia model of rabbits. METHODS: The tachyarrhythmia model was made by intravenous injection of barium chloride into the ears of rabbits. A total of 56 healthy adult male New Zealand big-eared white rabbits, apply the random number table method, divided into normal control group (group A), model group (group B), shallow needling Neiguan (PC6) 10 min group (group C), shallow needling Neiguan (PC6) 20 min group (group D), shallow needling Neiguan (PC6) 30 min group (group E), deep needling Neiguan (PC6) 10 min group (group F), deep needling Neiguan (PC6) 20 min group (group G), deep needling Neiguan (PC6) 30 min group (group H), 7 animals in each group. Electrocardiograms were used to collect the duration of arrhythmia; hematoxylin-eosin staining method was performed on myocardial tissue, RT-PCR tested the expression of α1C subunit mRNA, and the activity of Ca2 + -Mg2 + -ATPase were quantified by phosphorus determination method. RESULTS: The duration of arrhythmia in each acupuncture treatment group was shortened to varying degrees. Compare to the model group, the tissue damage from barium chloride inducing was improved in the acupuncture group. Compared to the model group, except for group E, most treatment groups had varying degrees of improvement with significantly down-regulated L-type calcium channel α1C subunit mRNA expressions level and increased Ca2+ -Mg2+ -ATPase activity. CONCLUSIONS: The effect of acupuncture at Neiguan (PC6) with different depths and retention time can reduce the duration of arrhythmia induced by barium chloride relatively, improve the induced pathological changes, down regulate L-type calcium channel α1C subunit mRNA expressions level and increase Ca2 + -Mg2 + -ATPase activity. Both the shallow and deep tissues of Neiguan (PC6) may be involved in transmitting acupuncture information. There is an optimal induction period for shallow needling at Neiguan (PC6) to reach the best therapeutic effect.

Expression Profile of Osteoclasts Following the Stimulation With Interleukin-23 in Mice.

OBJECTIVES: This study aims to analyze the expression profile of osteoclasts (OCs) following the stimulation with interleukin 23 (IL-23) in mice, which would imply the underlying effects of IL-23 on the function of OCs in inflammatory arthritis. MATERIALS AND METHODS: Mature OCs were induced from bone marrow mononuclear cells of 5 male mice (age 6 weeks; weighing 18-20 g) in the presence of macrophage-colony stimulating factor (50 ng/mL) and receptor activator of nuclear factor kappa B ligand (30 ng/mL) in vitro. The Agilent SurePrint G3 Mouse GE V2.0 Microarray was used to analyze the gene expression profile of OCs stimulated with IL-23 (30 ng/mL) or vehicle. The four major IL-23-modulated genes were validated by quantitative real-time polymerase chain reaction (qPCR) analysis. RESULTS: The expression levels of 23 genes were up-regulated and 32 genes were down-regulated by IL-23 stimulation (fold change ≥1.5 and p value <0.05). Among them, there were 37 genes with assigned gene symbols. Gene ontology analysis showed that the IL-23-regulated messenger ribonucleic acids (mRNAs) were related to positive regulation of leukocyte chemotaxis, chemokine-mediated signaling pathway and C-X-C chemokine receptors binding. The pathway analysis showed that the IL-23-regulated mRNAs were related to chemokine signaling pathway and cytokine-cytokine receptor interaction. The significant up-regulation of chemokine (C-X-C motif) ligand 1 and chemokine (C-X-C motif) ligand 2 induced by IL-23 was confirmed by qPCR. In addition, there were 18 long non-coding RNAs that were regulated by IL-23, while their function needs to be confirmed in the future. CONCLUSION: Expression levels of genes related to chemotaxis in OCs were up-regulated by IL-23 in mice, which imply that IL-23 may facilitate chemotaxis of OCs in inflammatory arthritis.

Adsorption and photocatalytic degradation of benzene compounds on acidic F-TiO2/SiO2 catalyst.

The adsorption and photocatalytic degradation performance of F-TiO2/SiO2 catalyst towards a series of benzene compounds were studied. The results revealed that the F-TiO2/SiO2 catalyst is superior to TiO2 P25 in adsorption capacity and photocatalytic degradation under simulant sunlight irradiation. The adsorptive capacity for chlorobenzene is the highest and the degradation rate is the greatest among these target pollutants. The increase of absorptive organic molecules on acidic F-TiO2/SiO2 catalyst benefits photocatalytic degradation. The photocatalytic reaction accords to Langmuir-Hinshelwood mechanism. The FTIR results indicated that the promoting effect of acidic centers on adsorption of benzene compounds depends on electron property of the functional groups. The electron-donating groups (-OH and -NH2) of benzene compounds are weakly adsorbed on acidic centers of the catalyst due to the competitive adsorption with H2O, while the electron-withdrawing groups (-Cl and -NO2) are adsorbed more strongly at acidic sites. The monosubstituted chlorobenzene prefers to perpendicular adsorption on acidic surface, while the disubstituted benzenes prefer to horizontal adsorption, which decreases the adsorbed amounts. A photocatalytic rate mainly depends on electron donating property of the functional group and amount of adsorptive organic molecules, but not on electron density of benzene ring.

Photocatalytic degradation of industrial acrylonitrile wastewater by F-S-Bi-TiO2 catalyst of ultrafine nanoparticles dispersed with SiO2 under natural sunlight.

Highly active photocatalyst, having certain anti-ionic interfering function, of F, S and Bi doped TiO2/SiO2 was used for the first time to degrade the organic pollutants in acrylonitrile industrial wastewater under natural sunlight. The photocatalyst were prepared and characterized by UV-Vis, XRD, TEM, EDS, Nitrogen physical adsorption and XPS technique. UV-Vis analysis revealed addition of F, S and Bi into the lattice of TiO2 led to the expansion of TiO2 response in the visible region and hence the efficient separation of charge carrier. The photocatalytic potential of as prepared catalyst to degrade acrylonitrile wastewater under simulated and natural sunlight irradiation was investigated. The extent of degradation of acrylonitrile wastewater was evaluated by chemical oxygen demand (CODCr). CODCr in wastewater decreased from 88.36 to 7.20 mgL-1 via 14 h irradiation of simulated sunlight and achieved regulation discharge by 6 h under natural sunlight, illuminating our photocatalyst effectiveness for refractory industrial wastewater treatment. From TEM results, we found that SiO2 could disperse the photocatalyst with different component distributions between the surface and the bulk phase that should also be responsible for the light absorption and excellent photocatalytic performance. The XPS analysis confirmed the presence of surface hydroxyl group, oxygen vacancies.

Fusing character strengths and mindfulness interventions: Benefits for job satisfaction and performance.

In recent years, both mindfulness and character strengths have started to garner interest in industrial and organizational psychology. The growing research interest in their effects on employee well-being and performance, individually, has strong practical implications for organizations. Given the interconnection of mindfulness and character strengths, the present study examined the effectiveness of training that combined the two practices regarding well-being and work-related outcomes, and it tested the potential mediators of the effects at work. A total of 63 participants from various job branches were randomly assigned to three conditions: (a) mindfulness-based strengths practice (MBSP), (b) mindfulness-based stress reduction (MBSR), and (c) wait-list control. Participants' applicability of character strengths at work, well-being, perceived stress, job satisfaction, and task performance (supervisor rating) were assessed before and after the intervention, and 1, 3, and 6 months afterward. A set of linear mixed-effects models was applied, modeling changes in participants' outcome variables over time. Potential mediators for the intervention effect of MBSP at work were tested using four criteria adapted from a previous study. Results showed the MBSR was effective for increasing well-being, reducing perceived stress, and increasing job satisfaction, whereas the MBSP was effective for increasing well-being, job satisfaction, and task performance. These findings suggest that mindfulness alone seems to function better when regarding well-being at work, while fusing character strengths on top of it seems to influence the participants, on a motivational level, and thus bolsters task performance. (PsycINFO Database Record (c) 2019 APA, all rights reserved).

The Mutual Support Model of Mindfulness and Character Strengths.

OBJECTIVES: Numerous studies have confirmed robust relationships between general well-being and mindfulness or character strengths, respectively, but few have examined associations between mindfulness and character strengths. Two studies were carried out to explore these relationships comprehensively in the framework of the Values in Action (VIA) classification of character strengths. METHODS: In study 1, participants (N = 1335) completed validated assessments of mindfulness and character strengths, and the relationship between the two was investigated in a broad online sample. In study 2, the effect of a mindfulness training on specific character strengths was investigated using a randomized-control design (N = 42). RESULTS: The results of study 1 confirmed positive relationships between mindfulness and character strengths and further identified a list of character strengths that might overlap with mindfulness-i.e., creativity, curiosity, open-mindedness, love of learning, perspective, bravery, perseverance, zest, love, social intelligence, forgiveness, self-regulation, appreciation of beauty, gratitude, hope, and spirituality. The findings of study 2 provided further support for the hypothesis that mindfulness training could help cultivate certain character strengths. Compared with participants in the waitlist control condition, those who attended an 8-week mindfulness-based training program showed significant increases in the strengths of love, appreciation of beauty, gratitude, and spirituality, and a trend toward significant increases in the strengths of zest and bravery. CONCLUSIONS: The results provide initial evidence for a mutual support model of mindfulness and character strengths.

Non-steroidal Anti-inflammatory Drugs Are Unlikely to Inhibit Radiographic Progression of Ankylosing Spondylitis: A Systematic Review.

Objective: To clarify if non-steroidal anti-inflammatory drugs (NSAIDs) could retard the disease progression of ankylosing spondylitis (AS). Methods: A systematic search of Embase, Pubmed, and the Cochrane Central Register of Controlled Trials (CCRCT) databases was conducted. Structural damage of AS was evaluated using spinal radiographs to assess modified Stoke Ankylosing Spondylitis Spine Score (mSASSS). Results: Five full-text papers (from 2 prospective and 2 retrospective studies) were included. Of the 4 studies deemed relevant, 3 reported no significant inhibition of spinal progression in AS patients treated continuously with NSAIDs, as determined by radiograph over 2-3 years. Only the 1st prospective randomized trial demonstrated that 2-year continuous use of celecoxib reduced mean changes in mSASSS of AS patients compared with on-demand treatment. However, the dosage difference of celecoxib between the two groups in the study seemed to be too small to elicit such differences in radiographic progression, while the therapy did not elicit any differences in disease activity, C-reactive protein (CRP) levels or global pain. Of the 3 studies that reported radiographic progression in the subgroup with elevated CRP, only post-hoc analysis of the 1st randomized study revealed that the patients treated continuously with NSAIDs had less radiological progression than those using on-demand NSAIDs. In 2 studies that reported radiographic progression in the patient subgroup with baseline syndesmophytes, both reported that there was no significant inhibition of progression of mSASSS in patients who had received continuous NSAID treatment compared with patients given on-demand NSAIDs. Conclusion: The available evidence suggests that NSAIDs are unable to delay radiographic progression of AS even in patients with elevated CRP levels.

Gallotannin 1,2,6-tri-O-galloyl-ß-d-glucopyranose: Its availability and changing patterns in tea (Camellia sinensis).

Gallotannin 1,2,6-tri-O-galloyl-ß-d-glucopyranose (1,2,6-TGGP) plays multiple roles against multidrug-resistant bacteria and other diseases. Nevertheless, its availability in tea (Camellia sinensis) has rarely been reported. Herein, the identification and verification of 1,2,6-TGGP from Camellia sinensis using ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-qTOF MS/MS), electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) were reported. The isolated 1,2,6-TGGP was used for the chemotaxonomy analysis of 17 tea cultivars. The contents of 1,2,6-TGGP ranged from 1.96 to 43.20 mg g-1, with a mean of 13.75 mg g-1. Relatively high 1,2,6-TGGP contents (>30 mg g-1) in two tea cultivars indicate that the beneficial effects of 1,2,6-TGGP can be obtained by consuming these teas. The chemotaxonomy analysis showed a biosynthetic relation between 1,2,6-TGGP and gallic acid. Further analysis showed that the 1,2,6-TGGP contents significantly decreased with the plucking times irrespective of the cultivars. Moreover, a positive and significant correlation was also observed between 1,2,6-TGGP and gallic acid. The identification of tea cultivars that are rich in 1,2,6-TGGP was first reported and the obtained results should boost their potential use in food and medicine.

An Initial Cross-Cultural Comparison of Adult Playfulness in Mainland China and German-Speaking Countries.

Compared with playfulness in infants and children, playfulness in adults is relatively under-studied. Although there is no empirical research comparing differences in adult playfulness across cultures, one might expect variations between Western and Eastern societies such as China. While playfulness is typically seen as a positive trait in Western culture, there are hints in Chinese culture that being playful has negative connotations (e.g., associations with laziness and seeing play as the opposite of work). The aim of this study was to compare expressions of playfulness in one sample from German-speaking countries (n = 143) and two samples from China (Guangzhou: n = 176; Beijing: n = 100). Participants completed one playfulness scale developed in the West (Short Measure of Adult Playfulness, SMAP) and one from the East (Adult Playfulness Questionnaire, APQ). Additional ratings of the participants were collected to measure: (a) the level of playful behavior expressed by people in different situations (e.g., when being around family members, in public, or on social media), and (b) individuals' perceptions of society's expectations concerning the appropriateness of being playful in the given situations. Overall, the results of the comparisons were mixed. Although SMAP scores did not vary significantly across the three samples, people from German-speaking countries tended to score higher on some facets of the APQ and some situational ratings. Stronger effects were found when comparing only the German-speaking sample and the Guangzhou sample. In addition to the cross-cultural differences that we expected, we also detected Chinese regional variations (North vs. South). We conclude that societal rules and cultural factors may impact expressions of playfulness in a society.

Identification of key genes involved in catechin metabolism in tea seedlings based on transcriptomic and HPLC analysis.

Tea is a non-alcoholic beverage with many benefits to human health and thereby widely consumed in the world. It contains plenty of secondary metabolites and tea catechins are the characteristic compounds. To further elucidate the biosynthetic and regulatory mechanisms of catechins in tea, high performance liquid chromatography (HPLC) and transcriptome analysis were performed in tea seedlings of different growth stages. A combined method of differential expression and correlation analysis was then conducted. The results showed that the order of total catechin (TC) contents was leaves > stems > roots, irrespective of growth stages. For transcriptome analysis, a total of 355.81 million clean reads were generated and mapped to the referencing tea genome. Further real time PCR analysis of 18 selected genes confirmed RNA-Seq results. A total of 7 structural genes and 35 transcription factors (TFs) were identified to be significantly correlated with TC changes. Among them, three TFs homologous to ANL2, WRKY44 and AtMYB113 might play key roles in catechin regulation. The de novo transcriptome data of different organs in tea seedlings provided new insights into the biosynthetic and metabolic pathways of catechins.

Antityrosinase and antioxidant properties of mung bean seed proanthocyanidins: Novel insights into the inhibitory mechanism.

This study investigated the structure, antioxidant activity, antityrosinase activity and mechanism of proanthocyanidins from mung bean seed [Vigna radiata (L.) Wilczek]. The structural composition were characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), electrospray ionization-full-mass spectrometry (ESI-Full-MS), and high-pressure liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) techniques. The mung bean seed proanthocyanidins were composed of procyanidins, prodelphinidins, and their rhamnosides. According to enzyme kinetic analysis, these compounds were potent, reversible, and mixed-type inhibitors of tyrosinase. They inhibited the enzyme activity by interacting with enzyme as well as substrates. The results of molecular docking showed that the interaction between mung bean seed proanthocyanidins and tyrosinase was driven by hydrogen bond, hydrophobic and electrostatic interactions. In addition, mung bean seed proanthocyanidins were demonstrated as powerful antioxidants. Therefore, this study confirmed a novel tyrosinase inhibitor and would lay a scientific foundation for their utilization in pharmaceutical and food industries.

Psychometric Comparisons of Benevolent and Corrective Humor across 22 Countries: The Virtue Gap in Humor Goes International.

Recently, two forms of virtue-related humor, benevolent and corrective, have been introduced. Benevolent humor treats human weaknesses and wrongdoings benevolently, while corrective humor aims at correcting and bettering them. Twelve marker items for benevolent and corrective humor (the BenCor) were developed, and it was demonstrated that they fill the gap between humor as temperament and virtue. The present study investigates responses to the BenCor from 25 samples in 22 countries (overall N = 7,226). The psychometric properties of the BenCor were found to be sufficient in most of the samples, including internal consistency, unidimensionality, and factorial validity. Importantly, benevolent and corrective humor were clearly established as two positively related, yet distinct dimensions of virtue-related humor. Metric measurement invariance was supported across the 25 samples, and scalar invariance was supported across six age groups (from 18 to 50+ years) and across gender. Comparisons of samples within and between four countries (Malaysia, Switzerland, Turkey, and the UK) showed that the item profiles were more similar within than between countries, though some evidence for regional differences was also found. This study thus supported, for the first time, the suitability of the 12 marker items of benevolent and corrective humor in different countries, enabling a cumulative cross-cultural research and eventually applications of humor aiming at the good.