Highly Flexible and Superelastic Graphene Nanofibrous Aerogels for Intelligent Sign Language.

Highly flexible and superelastic aerogels at large deformation have become urgent mechanical demands in practical uses, but both properties are usually exclusive. Here a trans-scale porosity design is proposed in graphene nanofibrous aerogels (GNFAs) to break the trade-off between high flexibility and superelasticity. The resulting GNFAs can completely recover after 1000 fatigue cycles at 60% folding strain, and notably maintain excellent structural integrity after 10000 cycles at 90% compressive strain, outperforming most of the reported aerogels. The mechanical robustness is demonstrated to be derived from the trans-scale porous structure, which is composed of hyperbolic micropores and porous nanofibers to enable the large elastic deformation capability. It is further revealed that flexible and superelastic GNFAs exhibit high sensitivity and ultrastability as an electrical sensors to detect tension and flexion deformation. As proof, The GNFA sensor is implemented onto a human finger and achieves the intelligent recognition of sign language with high accuracy by multi-layer artificial neural network. This study proposes a highly flexible and elastic graphene aerogel for wearable human-machine interfaces in sensor technology.

Adaptive-optics-based turbulence mitigation in a 400 Gbit/s free-space optical link by multiplexing Laguerre-Gaussian modes varying both radial and azimuthal spatial indices: publisher's note.

This publisher's note contains a correction to Opt. Lett.48, 6452 (2023)10.1364/OL.506270.

Exosome MiR-21-5p Upregulated by HIF-1α Induces Adipose Stem Cell Differentiation to Promote Ectopic Bone Formation.

Heterotopic bone occurs after burns, trauma and major orthopedic surgery, which cannot be completely cured by current treatments. The development of new treatments requires more in-depth research into the mechanism of HO. Available evidence suggests that miR-21-5p plays an important role in bone formation. However, its mechanism in traumatic HO is still unclear. First, we identified exosomes extracted from L6 cells using TEM observation of the structure and western blotting detection of the surface marker CD63. Regulation effect of HIF-1α to miR-21-5p was confirmed by q-PCR assay. Then we co-cultured L6 cells with ASCs and performed alizarin red staining and ALP detection. Overexpression of miR-21-5p upregulated BMP4, p-smad1/5/8, OCN and OPN, which suggests the BMP4-smad signaling pathway may be involved in miR-21-5p regulation of osteogenic differentiation of ASCs. Finally in vivo experiments showed that miR-21-5p exosomes promoted ectopic formation in traumatized mice. This study confirms that HIF-1α could modulate miR-21-5p exosomes to promote post-traumatic ectopic bone formation by inducing ASCs cell differentiation. Our study reveals the mechanisms of miR-21-5p in ectopic ossification formation after trauma.

Space-time wave packets with reduced divergence and tunable group velocity generated in free space after multi-mode fiber propagation.

Previously, space-time wave packets (STWPs) have been generated in free space with reduced diffraction and a tunable group velocity by combining multiple frequency comb lines each carrying a single Bessel mode with a unique wave number. It might be potentially desirable to propagate the STWP through fiber for reconfigurable positioning. However, fiber mode coupling might degrade the output STWP and distort its propagation characteristics. In this Letter, we experimentally demonstrate STWP generation and propagation over 1-m graded-index multi-mode fiber. Fiber mode coupling is mitigated by pre-distortion according to the inverse matrix of the fiber mode coupling matrix. Measurement of the STWP at the fiber output shows that its group velocity can vary from 1.0042c to 0.9967c by tuning the wave number of the Bessel mode on each frequency. The measured time-averaged intensity profiles show that the beam radius remains similar after 150-mm free-space propagation after exiting the fiber.

Adaptive-optics-based turbulence mitigation in a 400†Git/s free-space optical link by multiplexing Laguerre-Gaussian modes varying both radial and azimuthal spatial indices.

In general, atmospheric turbulence can degrade the performance of free-space optical (FSO) communication systems by coupling light from one spatial mode to other modes. In this Letter, we experimentally demonstrate a 400 Gbit/s quadrature-phase-shift-keyed (QPSK) FSO mode-division-multiplexing (MDM) coherent communication link through emulated turbulence using four Laguerre Gaussian (LG) modes with different radial and azimuthal indices (L G 10, L G 11, L G -10, and L G -11). To mitigate turbulence-induced channel cross talk and power loss, we implement an adaptive optics (AO) system at the receiver end. A Gaussian beam at a slightly different wavelength is co-propagated with the data beams as the probe beam. We use a wavefront sensor (WFS) to measure the wavefront distortion of this probe beam, and this information is used to tune a spatial light modulator (SLM) to adaptively correct the four distorted data-beam wavefronts. Using this adaptive-optics approach, the power loss and cross talk are reduced by ∼10 and ∼18 dB, respectively.

Molecular Insight into the Pozzolanic Reaction of Metakaolin and Calcium Hydroxide.

The reaction mechanism of the pozzolanic reaction of metakaolin (MK) from the atomic point of view has not yet been explored. To explain the process and mechanism of the pozzolanic reaction from the atomic point of view, molecular insight into the pozzolanic reaction of MK and calcium hydroxide (CH) was analyzed through the reaction molecular dynamics (MD) simulation. The results show that the pozzolanic reaction of MK and CH can be essentially regarded as the CH decomposition and penetration into MK. Also, the structure evolution after the pozzolanic reaction shows that the water molecules cannot penetrate the MK structure till the participation of Ca2+ and OH- ions of CH. The Ca2+ and OH- ions have strong interaction with MK and drill into the MK structure, followed by the destruction of a part of the MK structure and water penetration. The final structure of CH removed by MK can be regarded as the precursor of the CASH gel structure.

Performance of indocyanine green in sentinel lymph node mapping and lymph node metastasis in penile cancer: systematic review, meta-analysis, and single-center experience.

PURPOSE: The aim of this study was to investigate the overall sensitivity and specificity of indocyanine green (ICG)-near-infrared (NIR) fluorescence imaging in the detection of sentinel lymph node metastasis (SLNM) in penile cancer. METHODS: We searched PubMed, Embase, Web of Science, Scopus, and the Cochrane Library databases to identify manuscripts where ICG was intravenously administered prior to or during penile cancer surgery, with no restriction on language or publication status. The results extracted are presented as forest plots. RESULTS: Seven studies were included in the analysis. The median sensitivity and specificity of ICG-NIR imaging for SLNM detection were 100 and 4%, respectively; the pooled sensitivity was 100.0% (95% confidence interval [CI] 97.0-100.0) and specificity was 2.0% (95% CI 1.0-3.0). There was no significant difference in the diagnostic results between different injection sites and doses in each experimental group. CONCLUSION: To our knowledge, this meta-analysis is the first to summarize the diagnostic performance of ICG-NIR imaging for SLNM detection in penile cancer. ICG is sensitive in the imaging of SLN tissue, which can consequently improve the accuracy of lymph node detection. However, the specificity is very low.

Generation of OAM-carrying space-time wave packets with time-dependent beam radii using a coherent combination of multiple LG modes on multiple frequencies.

Space-time (ST) wave packets, in which spatial and temporal characteristics are coupled, have gained attention due to their unique propagation characteristics, such as propagation invariance and tunable group velocity in addition to their potential ability to carry orbital angular momentum (OAM). Through experiment and simulation, we explore the generation of OAM-carrying ST wave packets, with the unique property of a time-dependent beam radius at various ranges of propagation distances. To achieve this, we synthesize multiple frequency comb lines, each assigned to a coherent combination of multiple Laguerre-Gaussian (LGℓ,p) modes with the same azimuthal index but different radial indices. The time-dependent interference among the spatial modes at the different frequencies leads to the generation of the desired OAM-carrying ST wave packet with dynamically varying radii. The simulation results indicate that the dynamic range of beam radius oscillations increases with the number of modes and frequency lines. The simulated ST wave packet for OAM of orders +1 or +3 has an OAM purity of >95%. In addition, we experimentally generate and measure the OAM-carrying ST wave packets with time-dependent beam radii. In the experiment, several lines of a Kerr frequency comb are spatially modulated with the superposition of multiple LG modes and combined to generate such an ST wave packet. In the experiment, ST wave packets for OAM of orders +1 or +3 have an OAM purity of >64%. In simulation and experiment, OAM purity decreases and beam radius becomes larger over the propagation.

Utilizing multiplexing of structured THz beams carrying orbital-angular-momentum for high-capacity communications.

Structured electromagnetic (EM) waves have been explored in various frequency regimes to enhance the capacity of communication systems by multiplexing multiple co-propagating beams with mutually orthogonal spatial modal structures (i.e., mode-division multiplexing). Such structured EM waves include beams carrying orbital angular momentum (OAM). An area of increased recent interest is the use of terahertz (THz) beams for free-space communications, which tends to have: (a) larger bandwidth and lower beam divergence than millimeter-waves, and (b) lower interaction with matter conditions than optical waves. Here, we explore the multiplexing of THz OAM beams for high-capacity communications. Specifically, we experimentally demonstrate communication systems with two multiplexed THz OAM beams at a carrier frequency of 0.3 THz. We achieve a 60-Gbit/s quadrature-phase-shift-keying (QPSK) and a 24-Gbit/s 16 quadrature amplitude modulation (16-QAM) data transmission with bit-error rates below 3.8 × 10-3. In addition, to show the compatibility of different multiplexing approaches (e.g., polarization-, frequency-, and mode-division multiplexing), we demonstrate an 80-Gbit/s QPSK THz communication link by multiplexing 8 data channels at 2 polarizations, 2 frequencies, and 2 OAM modes.

Synthesis of near-diffraction-free orbital-angular-momentum space-time wave packets having a controllable group velocity using a frequency comb.

Novel forms of light beams carrying orbital angular momentum (OAM) have recently gained interest, especially due to some of their intriguing propagation features. Here, we experimentally demonstrate the generation of near-diffraction-free two-dimensional (2D) space-time (ST) OAM wave packets (ℓ = +1, +2, or +3) with variable group velocities in free space by coherently combining multiple frequency comb lines, each carrying a unique Bessel mode. Introducing a controllable specific correlation between temporal frequencies and spatial frequencies of these Bessel modes, we experimentally generate and detect near-diffraction-free OAM wave packets with high mode purities (>86%). Moreover, the group velocity can be controlled from 0.9933c to 1.0069c (c is the speed of light in vacuum). These ST OAM wave packets might find applications in imaging, nonlinear optics, and optical communications. In addition, our approach might also provide some insights for generating other interesting ST beams.

Turbulence-resilient pilot-assisted self-coherent free-space optical communications using a photodetector array for bandwidth enhancement.

We experimentally demonstrate a 4-Gbit/s 16-QAM pilot-assisted, self-coherent, and turbulence-resilient free-space optical link using a photodetector (PD) array. The turbulence resilience is enabled by the efficient optoelectronic mixing of the data and pilot beams in a free-space-coupled receiver, which can automatically compensate for turbulence-induced modal coupling to recover the data's amplitude and phase. For this approach, a sufficient PD area might be needed to collect the beams while the bandwidth of a single larger PD could be limited. In this work, we use an array of smaller PDs instead of a single larger PD to overcome the beam collection and bandwidth response trade-off. In the PD-array-based receiver, the data and pilot beams are efficiently mixed in the aggregated PD area formed by four PDs, and the four mixing outputs are electrically combined for data recovery. The results show that: (i) either with or without turbulence effects (D/r0 = ∼8.4), the 1-Gbaud 16-QAM signal recovered by the PD array has a lower error vector magnitude than that of a single larger PD; (ii) for 100 turbulence realizations, the pilot-assisted PD-array receiver recovers 1-Gbaud 16-QAM data with a bit-error rate below 7% of the forward error correction limit; and (iii) for 1000 turbulence realizations, the average electrical mixing power loss of a single smaller PD, a single larger PD, and a PD array is ∼5.5 dB, ∼1.2 dB, and ∼1.6 dB, respectively.

Tunability of space-time wave packet carrying tunable and dynamically changing OAM value.

Space-time (ST) wave packets have gained much interest due to their dynamic optical properties. Such wave packets can be generated by synthesizing frequency comb lines, each having multiple complex-weighted spatial modes, to carry dynamically changing orbital angular momentum (OAM) values. Here, we investigate the tunability of such ST wave packets by varying the number of frequency comb lines and the combinations of spatial modes on each frequency. We experimentally generate and measure the wave packets with tunable OAM values from +1 to +6 or from +1 to +4 during a ∼5.2-ps period. We also investigate, in simulation, the temporal pulse width of the ST wave packet and the nonlinear variation of the OAM values. The simulation results show that: (i) a pulse width can be narrower for the ST wave packet carrying dynamically changing OAM values using more frequency lines; and (ii) the nonlinearly varying OAM value can result in different frequency chirps along the azimuthal direction at different time instants.

Demonstration of turbulence mitigation in a 200-Gbit/s orbital-angular-momentum multiplexed free-space optical link using simple power measurements for determining the modal crosstalk matrix.

We experimentally demonstrate turbulence mitigation in a 200-Gbit/s quadrature phase-shift keying (QPSK) orbital-angular-momentum (OAM) mode-multiplexed system using simple power measurements for determining the modal coupling matrix. To probe and mitigate turbulence, we perform the following: (i) sequentially transmit multiple probe beams at 1550-nm wavelength each with a different combination of Laguerre-Gaussian (LG) modes; (ii) detect the power coupling of each probe beam to LG0,0 for determining the complex modal coupling matrix; (iii) calculate the conjugate phase of turbulence-induced spatial phase distortion; (iv) apply this conjugate phase to a spatial light modulator (SLM) at the receiver to mitigate the turbulence distortion for the 1552-nm mode-multiplexed data-carrying beams. The probe wavelength is close enough to the data wavelength such that it experiences similar turbulence, but is far enough away such that the probe beams do not affect the data beams and can all operate simultaneously. Our experimental results show that with our turbulence mitigation approach the following occur: (a) the inter-channel crosstalk is reduced by ∼25 and ∼21 dB for OAM +1 and -2 channels, respectively; (b) the optical signal-to-noise ratio (OSNR) penalty is <1 dB for both OAM channels for a bit error rate (BER) at the 7% forward error correction (FEC) limit, compared with the no turbulence case.

Cardiopulmonary exercise test-based assessment of the effects of sacubitril/valsartan on the blood pressure response to exercise in patients with acute myocardial infarction during hospitalization.

OBJECTIVE: To investigate the effects of sacubitril/valsartan (S/V) on cardiopulmonary function and blood pressure response to exercise during hospitalization in patients with acute myocardial infarction (AMI) based on the cardiopulmonary exercise test (CPET). METHODS: A total of 265 AMI patients were treated with either perindopril or S/V within 24 hours of admission. CPET was completed for all patients before discharge. There were 182 cases in the perindopril group and 83 cases in the S/V group. RESULTS: The proportion of exercise oscillatory ventilation (EOV) was higher in the S/V group than in the perindopril group (10.8% vs 1.6%, X2 = 11.148, P = .001). The resting heart rate (HR), resting diastolic blood pressure (DBP), and warm-up DBP were lower in the S/V group than in the perindopril group (P < .05). The resting systolic blood pressure (SBP) was 9.0 mmHg lower (115.7 ± 17.5 vs 106.7 ± 15.0, P < .001), the SBP during warm-up was 9.5 mmHg lower (124.8 ± 23.7 vs 115.3 ± 22.5,P = .002), the SBP at the anaerobic threshold (AT) was 10.5 mmHg lower (135.3 ± 24.8 vs 127.1 ± 25.1,P = .021),the SBP at max watts was 11.5 mmHg lower (148.9 ± 26.4 vs 137.4 ± 26.4,P = .001), and the SBP during one-minute recovery was 12.3 mmHg lower (146.5 ± 27.1 vs 134.2 ± 24.4, P = .001)in the S/V group than in the perindopril group. The S/V group had a higher oxygen ventilation equivalent and carbon dioxide ventilation equivalent (VE/VCO2) at AT and a lower oxygen uptake-work rate relationship during max watts (P < .05). The differences in the oxygen pulse, stroke volume, peak oxygen uptake (VO2 peak), and VE/VCO2 slope were not statistically significant between the two groups. CONCLUSION: Treatment with S/V was able to reduce the exercise blood pressure in patients with AMI during hospitalization, but did not significantly improve the VO2 peak, VE/VCO2 slope, or exercise tolerance.

Adiabatic Frequency Conversion Using a Time-Varying Epsilon-Near-Zero Metasurface.

A time-dependent change in the refractive index of a material leads to a change in the frequency of an optical beam passing through that medium. Here, we experimentally demonstrate that this effect-known as adiabatic frequency conversion (AFC)-can be significantly enhanced by a nonlinear epsilon-near-zero-based (ENZ-based) plasmonic metasurface. Specifically, by using a 63-nm-thick metasurface, we demonstrate a large, tunable, and broadband frequency shift of up to ∼11.2 THz with a pump intensity of 4 GW/cm2. Our results represent a decrease of ∼10 times in device thickness and 120 times in pump peak intensity compared with the cases of bare, thicker ENZ materials for the similar amount of frequency shift. Our findings might potentially provide insights for designing efficient time-varying metasurfaces for the manipulation of ultrafast pulses.

Real-time monitoring of single circulating tumor cells with a fluorescently labeled deoxy-glucose by in vivo flow cytometry.

Circulating tumor cells (CTCs) play an essential role in metastasis and serve as an important prognostic biomarker. The technology of CTC labeling and detection in vivo can greatly improve the research of cancer metastasis and therapy. However, there is no in vivo technology to detect CTCs in clinic. In this study, we demonstrate that 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-d-glucose (2-NBDG), a 2-deoxy-glucose analog, can work in vivo to indicate CTCs and metastases fluorescently by direct intravenous injection. During the development of an implanted tumor in mice, the spontaneous CTCs released from the primary tumor into blood vessels can be labeled by 2-NBDG due to the abnormal metabolism of CTCs. The green fluorescence of 2-NBDG from CTCs is then noninvasively detected by an in vivo flow cytometry system. Due to the high uptake of glucose by tumor cells, the CTCs in mice can maintain a high 2-NBDG level and thus be distinguished by 2-NBDG fluorescence in vivo efficiently, enabling tumor detection in vivo like positron emission tomography (PET) but at the single-cell resolution. Our results suggest 2-NBDG, a glucose analog with high biosafety, holds promising potential in clinical applications, similar to the widely-used contrast medium 2-F18 -fluorodeoxyglucose in PET.

Modal properties of a beam carrying OAM generated by a circular array of multiple ring-resonator emitters.

We investigate the modal properties of a beam carrying orbital angular momentum (OAM) generated by a circular array (ring) of multiple micro-ring emitters (rings) analytically and via simulation. In such a "ring-of-rings" structure, N emitters generate N optical vortex beams with the same OAM-order l0 at the same wavelength. The output beam is a coherent combination of the N vortex beams located at different azimuthal positions, having the same radial displacement. We derive an analytical expression for the output optical field and calculate the OAM-order power spectrum of the generated beam. The analytical expression and simulation results show that (1) the OAM spectrum of the output beam composes equidistant OAM spectral components, symmetrically surrounding l0 with a spacing equal to N; (2) the envelope of the OAM spectrum broadens with an increased radius of the circular array or the value of l0; and (3) the OAM components of the generated beam could be tuned either by changing the value of l0, corresponding to different spectrum envelopes, or by adding different linear phase delays to the micro-ring emitters, which does not affect the envelope of the OAM spectrum.

Demonstration of generating a 100 Gbit/s orbital-angular-momentum beam with a tunable mode order over a range of wavelengths using an integrated broadband pixel-array structure.

We experimentally generate an orbital-angular-momentum (OAM) beam with a tunable mode order over a range of wavelengths utilizing an integrated broadband pixel-array OAM emitter. The emitter is composed of a 3-to-4 coupler, four phase controllers, and a mode convertor. An optical input is split into four waveguides by the coupler. Subsequently, the four waveguide fields are coherently combined and transformed into a free-space OAM beam by the mode convertor. By tuning the phase delay Δφ between the four waveguides using the integrated phase controllers, the OAM order of the generated beam could be changed. Our results show that (a) a single OAM beam with a tunable OAM order (ℓ=-1 or ℓ=+1) is generated with the intermodal power coupling of <-11dB, and (b) in a wavelength range of 6.4 nm, a free-space link of a single 50 Gbaud quadrature-phase-shift-keying (QPSK) channel carried by the tunable OAM beam is achieved with a bit error rate below the forward-error-correction threshold. As proof of concept, a 400 Gbit/s OAM-multiplexed and WDM QPSK link is demonstrated with a ∼1-dB OSNR penalty compared with a single-beam link.

Simulation of near-diffraction- and near-dispersion-free OAM pulses with controllable group velocity by combining multiple frequencies, each carrying a Bessel mode.

Optical pulses carrying orbital angular momentum (OAM) have recently gained interest. In general, it might be beneficial to simultaneously achieve: (i) minimum diffraction, (ii) minimum dispersion, and (iii) controllable group velocity. Here, we explore via simulation the generation of near-diffraction-free and near-dispersion-free OAM pulses with arbitrary group velocities by coherently combining multiple frequencies. Each frequency carries a specific Bessel mode with the same topological charge (ℓ) but different kr (spatial frequency) values based on space-time correlations. Moreover, we also find that (i) both positive and negative group velocities could be achieved and continuously controlled from the subluminal to superluminal values and (ii) when the ℓ is varied from 0 to 10, the simulated value of the group velocity remains the same. However, as the ℓ value increases, the pulse duration becomes longer for a given number of frequency lines.

Tunable Doppler shift using a time-varying epsilon-near-zero thin film near 1550 nm.

We experimentally investigate the tunable Doppler shift in an 80 nm thick indium-tin-oxide (ITO) film at its epsilon-near-zero (ENZ) region. Under strong and pulsed excitation, ITO exhibits a time-varying change in the refractive index. A maximum frequency redshift of 1.8 THz is observed in the reflected light when the pump light has a peak intensity of ∼140GW/cm2 and a pulse duration of ∼580fs, at an incident angle of 40°. The frequency shift increases with the increase in pump intensity and saturates at the intensity of ∼140GW/cm2. When the pump pulse duration increases from ∼580fs to ∼1380fs, the maximum attainable frequency shift decreases from 1.8 THz to 0.7 THz. In addition, the pump energy required to saturate the frequency shift decreases with the increase in pump pulse duration for ∼x<1ps and remains unchanged for ∼x>1ps durations. Tunability exists among the pump pulse energy, duration, and incident angle for the Doppler shift of the ITO-ENZ material, which can be employed to design efficient frequency shifters for telecom applications.