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1.
Angew Chem Int Ed Engl ; 62(34): e202304244, 2023 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-37366041

RESUMO

In many industrially important reactions, caustic mineral acid catalysts have been successfully replaced with green solid acids such as zeolites. In this context, extensive efforts have been devoted to replacing HCl to produce methylenedianiline (MDA), a key intermediate in polyurethane production. Unfortunately, limited success has been achieved thus far due to low activity, selectivity towards the desired 4,4'-MDA, and rapid catalyst deactivation. Here we report that meso-/microporous hierarchical LTL zeolite exhibits unprecedentedly high activity, selectivity, and stability. The one-dimensional cage-like micropores of LTL promote the bimolecular reaction between two para-aminobenzylaniline intermediates to selectively produce 4,4'-MDA and inhibit the formation of undesired isomers and heavy oligomers. Meanwhile, the secondary mesopores alleviate mass transfer limitations, resulting in a 7.8-fold higher MDA formation rate compared to solely microporous LTL zeolite. Due to suppressed oligomer formation and fast mass transfer, the catalyst exhibits inappreciable deactivation in an industrially relevant continuous flow reactor.

2.
Angew Chem Int Ed Engl ; 60(22): 12482-12489, 2021 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-33729643

RESUMO

Controlling metal-support interactions is important for tuning the catalytic properties of supported metal catalysts. Here, premade Pd particles are supported on stable polymers containing different ligating functionalities to control the metal-polymer interactions and their catalytic properties in industrially relevant acetylene partial hydrogenation. The polymers containing strongly ligating groups (e.g., Ar-SH and Ar-S-Ar) can form a polymer overlayer on the Pd surface, which enables selective acetylene adsorption and partial hydrogenation to ethylene without deactivation. In contrast, polymers with weakly ligating groups (e.g., Ar-O-Ar) do not form an overlayer, resulting in non-selective hydrogenation and fast deactivation, similar to Pd catalysts on conventional inorganic supports. The results imply that tuning the metal-polymer interactions via rational polymer design can provide an efficient way of synthesizing selective and stable catalysts for hydrogenation.

4.
Sensors (Basel) ; 16(8)2016 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-27548172

RESUMO

The recently developed smart strand can be used to measure the prestress force in the prestressed concrete (PSC) structure from the construction stage to the in-service stage. The higher cost of the smart strand compared to the conventional strand renders it unaffordable to replace all the strands by smart strands, and results in the application of only a limited number of smart strands in the PSC structure. However, the prestress forces developed in the strands of the multi-strand system frequently adopted in PSC structures differ from each other, which means that the prestress force in the multi-strand system cannot be obtained by simple proportional scaling using the measurement of the smart strand. Therefore, this study examines the prestress force distribution in the multi-strand system to find the correlation between the prestress force measured by the smart strand and the prestress force distribution in the multi-strand system. To that goal, the prestress force distribution was measured using electromagnetic sensors for various factors of the multi-strand system adopted on site in the fabrication of actual PSC girders. The results verified the possibility to assume normal distribution for the prestress force distribution per anchor head, and a method computing the mean and standard deviation defining the normal distribution is proposed. This paper presents a meaningful finding by proposing an estimation method of the prestress force based upon field-measured data of the prestress force distribution in the multi-strand system of actual PSC structures.

5.
Molecules ; 21(3): 293, 2016 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-26985889

RESUMO

The global burden of bacterial infections is very high and has been exacerbated by increasing resistance to multiple antibiotics. Antibiotic resistance leads to failed treatment of infections, which can ultimately lead to death. To overcome antibiotic resistance, it is necessary to identify new antibacterial agents. In this study, a total of 662 plant extracts (diverse parts) from 222 plant species (82 families, 177 genera) were screened for antibacterial activity using the agar cup plate method. The aqueous and methanolic extracts were prepared from diverse plant parts and screened against eight bacterial (two Gram-positive and six Gram-negative) species, most of which are involved in common infections with multiple antibiotic resistance. The methanolic extracts of several plants were shown to have zones of inhibition ≥ 12 mm against both Gram-positive and Gram-negative bacteria. The minimum inhibitory concentration was calculated only with methanolic extracts of selected plants, those showed zone of inhibition ≥ 12 mm against both Gram-positive and Gram-negative bacteria. Several extracts had minimum inhibitory concentration ≤ 1 mg/mL. Specifically Adhatoda vasica, Ageratum conyzoides, Alangium salvifolium, Alpinia galanga, Andrographis paniculata, Anogeissus latifolia, Annona squamosa, A. reticulate, Azadirachta indica, Buchanania lanzan, Cassia fistula, Celastrus paniculatus, Centella asiatica, Clausena excavate, Cleome viscosa, Cleistanthus collinus, Clerodendrum indicum, Croton roxburghii, Diospyros melanoxylon, Eleutherine bulbosa, Erycibe paniculata, Eryngium foetidum, Garcinia cowa, Helicteres isora, Hemidesmus indicus, Holarrhena antidysenterica, Lannea coromandelica, Millettia extensa, Mimusops elengi, Nyctanthes arbor-tristis, Oroxylum indicum, Paederia foetida, Pterospermum acerifolium, Punica granatum, Semecarpus anacardium, Spondias pinnata, Terminalia alata and Vitex negundo were shown to have significant antimicrobial activity. The species listed here were shown to have anti-infective activity against both Gram-positive and Gram-negative bacteria. These results may serve as a guide for selecting plant species that could yield the highest probability of finding promising compounds responsible for the antibacterial activities against a broad spectrum of bacterial species. Further investigation of the phytochemicals from these plants will help to identify the lead compounds for drug discovery.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Plantas Medicinais/química , Antibacterianos/isolamento & purificação , Avaliação Pré-Clínica de Medicamentos , Farmacorresistência Bacteriana , Índia , Medicina Tradicional , Testes de Sensibilidade Microbiana
6.
Sensors (Basel) ; 15(1): 1060-70, 2015 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-25580903

RESUMO

Prestressed Concrete Wire and Strand (PC) strands are the most used materials to introduce prestress in a Pre-Stressed Concrete (PSC) structure. However, it is difficult to evaluate the final prestress force of the PC strand after prestressing or its residual prestress force after completion of the structure on site. This impossibility to assess eventual loss of prestress of the PC strand has resulted in a number of serious accidents and even in the collapse of several structures. This situation stresses the necessity to maintain the prestress force residual or after prestressing for the evaluation of the health of the concrete structure throughout its lifespan. Recently, several researchers have studied methods enabling one to verify the prestress force by inserting an optical fiber sensor inside the strand but failed to provide simple techniques for the fabrication of these devices to fulfill measurement performance from the design prestress to failure. Moreover, these methods require the additional installation of electrical resistance strain gages, displacement sensors and load cells on the outer surface of the structure for long-term precise measurement. This paper proposes a method enabling one to evaluate precisely and effectively the prestress force of the PC strand and intends to verify the applicability of the proposed method on actual concrete structures. To that end, an innovative PC strand is developed by embedding a Fiber Bragg Grating (FBG) sensor in the core wire of the PC strand so as to enable short term as well as long term monitoring. The measurement performance of the developed strand is then evaluated experimentally and the reliability of the monitoring data is assessed.

7.
Sensors (Basel) ; 15(6): 14079-92, 2015 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-26083230

RESUMO

Prestressed concrete (PSC) is one of the most reliable, durable and widely used construction materials, which overcomes the weakness of concrete in tension by the introduction of a prestress force. Smart strands enabling measurement of the prestress force have recently been developed to maintain PSC structures throughout their lifetime. However, the smart strand cannot give a representative indication of the whole prestress force when used in multi-strand systems since each strand sustains a different prestress force. In this paper, the actual distribution of the prestress force in a multi-strand system is examined using elastomagnetic (EM) sensors to develop a method for tracking representative indicators of the prestress force using smart strands.

8.
Diabetes Metab J ; 48(2): 253-264, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38273791

RESUMO

BACKGRUOUND: We investigated the long-term efficacy and safety of initial triple therapy using metformin, a dipeptidyl peptidase-4 inhibitor, and a sodium-glucose cotransporter-2 inhibitor, in patients with type 2 diabetes mellitus. METHODS: We enrolled 170 drug-naïve patients with glycosylated hemoglobin (HbA1c) level >7.5% who had started triple therapy (metformin, sitagliptin, and empagliflozin). Glycemic, metabolic, and urinary parameters were measured for 24 months. RESULTS: After 24 months, HbA1c level decreased significantly from 11.0%±1.8% to 7.0%±1.7%. At 12 and 24 months, the rates of achievement of the glycemic target goal (HbA1c <7.0%) were 72.5% and 61.7%, respectively, and homeostasis model assessment of ß-cell function and insulin resistance indices improved. Whole-body fat percentage decreased by 1.08%, and whole-body muscle percentage increased by 0.97% after 24 months. Fatty liver indices and albuminuria improved significantly. The concentration of ketone bodies was elevated at the baseline but decreased after 24 months. There were no serious adverse events, including ketoacidosis. CONCLUSION: Initial triple combination therapy with metformin, sitagliptin, and empagliflozin led to achievement of the glycemic target goal, which was maintained for 24 months without severe hypoglycemia but with improved metabolic function and albuminuria. This combination therapy may be a good strategy for drug-naïve patients with type 2 diabetes mellitus.


Assuntos
Compostos Benzidrílicos , Diabetes Mellitus Tipo 2 , Glucosídeos , Metformina , Inibidores do Transportador 2 de Sódio-Glicose , Humanos , Diabetes Mellitus Tipo 2/metabolismo , Metformina/efeitos adversos , Fosfato de Sitagliptina/efeitos adversos , Hemoglobinas Glicadas , Inibidores do Transportador 2 de Sódio-Glicose/uso terapêutico , Albuminúria , Glicemia/metabolismo , Resultado do Tratamento
9.
Clin Nutr ; 43(5): 1117-1124, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38582014

RESUMO

BACKGROUND & AIMS: The relationship between diet and health, particularly the role of carbohydrates, has been extensively studied. However, carbohydrate intake based on individual health conditions remains unclear. Here, we aimed to investigate whether the association between carbohydrate intake and all-cause mortality varied between individuals with and without diabetes mellitus (DM). METHODS: This prospective cohort study used data from the Korean Genome and Epidemiology Study (KoGES). Overall, 143,050 participants were included, with 10.1% having DM. Dietary intake was assessed using a semiquantitative food frequency questionnaire. Cox proportional hazards regression models were used to assess the association between carbohydrate intake and mortality after adjusting for confounders. RESULTS: The study showed that 5436 deaths occurred during the median follow-up period of 10.1 years. A significant interaction between carbohydrate intake and DM was observed in the study population (interaction p = 0.061). Higher carbohydrate intake proportion was associated with an increased risk of all-cause mortality among individuals with DM (adjusted hazard ratio [HR], p-value = 1.10 [1.01-1.20], p = 0.032). Conversely, no association was observed between the proportion of carbohydrate intake and all-cause mortality in participants without DM. Additionally, both total sugar and added sugar intakes were associated with an increased risk of all-cause mortality in participants with DM (adjusted HR, p-value = 1.02 [1.01-1.04], p < 0.001 and 1.18 [1.13-1.24], p < 0.001). CONCLUSIONS: High carbohydrate (%) and added sugar intake were associated with an increased mortality risk in individuals with DM. Reducing carbohydrate intake and opting for healthy carbohydrates to mitigate mortality risk may be beneficial for individuals with DM, particularly when compared with the general population.


Assuntos
Diabetes Mellitus , Carboidratos da Dieta , Humanos , Estudos Prospectivos , Masculino , Feminino , Pessoa de Meia-Idade , Carboidratos da Dieta/administração & dosagem , República da Coreia/epidemiologia , Diabetes Mellitus/mortalidade , Diabetes Mellitus/epidemiologia , Idoso , Modelos de Riscos Proporcionais , Fatores de Risco , Dieta/estatística & dados numéricos , Causas de Morte
10.
J Nanosci Nanotechnol ; 13(4): 2708-13, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23763148

RESUMO

In this study, the Poly(vinylidene fluoride-trifluoethylene) (PVDF) electrospun fibers were successfully prepared by electrospinning. Processing parameters, such as solvents and solution temperature were varied to study their influence on fiber dimensions. Electrospun PVDF fibers were characterized by scanning electron microscope (SEM), Fourier transform infrared spectrophotometer (FT-IR), wide angle X-ray diffraction (WAXD) and differential scanning calorimetry (DSC). The result indicated that the solvent component and temperature have great influence on fiber dimensions. 19% PVDF dissolved in DMF/MEK mixed solvents with the ratio of 8:2 was considered to be most suitable in this study. Furthermore, the increasing of solution temperature can probably induce the formation of beta-phases in electrospun PVDF Fibers.

11.
J Mater Sci Mater Med ; 24(8): 2029-36, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23661256

RESUMO

In this work, novel poly(ε-caprolactone) (PCL) fibrous membranes incorporating amphiphilic polyhedral oligosilsesquioxane (POSS) telechelic (PEG-POSS telechelic) were prepared via electrospinning. The unique microstructure, morphology, thermal stability of the resulting PCL/PEG-POSS telechelic electrospun nanowebs were investigated by X-ray diffraction, scanning electron microscopy, and thermogravimetric analysis, respectively. The addition of amphiphilic PEG-POSS telechelic strongly influenced the fiber diameters, microstructures of the resultant PCL/PEG-POSS telechelic nanofibers, compared to pure PCL nanofibers. The potential biomedical applications of such PEG-POSS telechelic nanowebs as a scaffolding material were also evaluated in vitro using mouse osteoblast-like MC3T3-E1 cells. The cell adhesion, spreading, and interaction behavior of pure PCL and PCL/PEG-POSS telechelic fibrous membranes were explored. It was found that electrospun PCL fibrous membranes incorporating amphiphilic PEG-POSS telechelic showed higher initial cell attachment than pure PCL due to the higher surface free energy of POSS siloxanes. Moreover, the obtained PCL/PEG-POSS telechelic fibrous scaffolds were found to be nontoxic and to maintain the good adhesion ratio between cells and surface (about ~93 %) after cell culturing for 24 h.


Assuntos
Compostos de Organossilício/química , Osteoblastos/citologia , Poliésteres/química , Polietilenoglicóis/química , Alicerces Teciduais , Animais , Adesão Celular/efeitos dos fármacos , Técnicas de Cultura de Células/instrumentação , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Galvanoplastia/métodos , Camundongos , Compostos de Organossilício/farmacologia , Osteoblastos/efeitos dos fármacos , Osteoblastos/fisiologia , Poliésteres/síntese química , Polietilenoglicóis/farmacologia , Tensoativos/química , Tensoativos/farmacologia , Alicerces Teciduais/química , Difração de Raios X
12.
ScientificWorldJournal ; 2013: 421578, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24204204

RESUMO

The purpose of this study is to clone and characterize the expression pattern of a F5H gene encoding ferulate 5-hydroxylase in the phenylpropanoid pathway from kenaf (Hibiscus cannabinus L.). Kenaf is a fast-growing dicotyledonous plant valued for its biomass. F5H, a cytochrome P450-dependent monooxygenase (CYP84), is a key enzyme for syringyl lignin biosynthesis. The full length of the F5H ortholog was cloned and characterized. The full-length F5H ortholog consists of a 1,557-bp open reading frame (ORF) encoding 518 amino acids (GenBank Accession number JX524278). The deduced amino acid sequence showed that kenaf F5H had the highest similarity (78%) with that of Populus trichocarpa. Transcriptional analysis of F5H ortholog was conducted using quantitative real-time PCR during the developmental stages of various tissues and in response to various abiotic stresses. The highest transcript level of the F5H ortholog was observed in immature flower tissues and in early stage (6 week-old) of stem tissues, with a certain level of expression in all tissues tested. The highest transcript level of F5H ortholog was observed at the late time points after treatments with NaCl (48 h), wounding (24 h), cold (24 h), abscisic acid (24 h), and methyl jasmonate (24 h).


Assuntos
Sistema Enzimático do Citocromo P-450/genética , Flores/metabolismo , Regulação da Expressão Gênica de Plantas/fisiologia , Hibiscus/enzimologia , Oxigenases de Função Mista/genética , Estresse Fisiológico/genética , Ácido Abscísico , Acetatos , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Ciclopentanos , Sistema Enzimático do Citocromo P-450/metabolismo , Primers do DNA/genética , Flores/genética , Perfilação da Expressão Gênica , Hibiscus/genética , Lignina/biossíntese , Oxigenases de Função Mista/metabolismo , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , Oxilipinas , Reação em Cadeia da Polimerase em Tempo Real , Análise de Sequência de DNA , Homologia de Sequência , Cloreto de Sódio
13.
J Spinal Disord Tech ; 24(5): 325-33, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20975591

RESUMO

STUDY DESIGN: A retrospective study. OBJECTIVES: To evaluate clinical and functional success by all pedicle screw construct in paralytic neuromuscular scoliosis (NMS) with poor pulmonary functions (PFT). SUMMARY OF BACKGROUND: Duchene muscular dystrophy and spinal muscular atrophy are often associated with poor PFT and the development of scoliosis simultaneously. Poor PFT often make surgeons reluctant to operate. METHODS: Eighteen paralytic NMS patients who had preoperative forced vital capacity (FVC) < 30% were operated with all pedicle screw construct. Average preoperative, postoperative, and final follow-up Cobb angle, pelvic obliquity, thoracic kyphosis and lumbar lordosis, PFT (FVC% and forced expiratory volume 1%), and preoperative and follow-up functional status were analyzed. Perioperative and postoperative complications were also noted. RESULTS: The average follow-up was 31.6 ± 7.7 months. There was significant improvement in Cobb angle (61.7%) and pelvic obliquity (56.7%), postoperatively (P < 0.001). All corrections were maintained at final follow-up. FVC was decreased from 25.2 ± 4.7% preoperatively to 24.2 ± 5.0%, 6 weeks postoperatively (P = 0.067); and on follow-up it further decreased to 20.6 ± 3.9% (P < 0.0001) (1.8%/y). Forced Expiratory Volume 1 also decreased from 22.7 ± 4.5% preoperatively to 21.8 ± 4.2% postoperatively (P = 0.037) and was 19.8 ± 3.8% at final follow-up (P < 0.0001) (1.1%/y). However, none of the patients had any respiratory complications postoperatively. Functional status was improved in 6 patients and they were able to sit without support (P = 0.027). Eight (44.4%) perioperative complications (5 pulmonary, 1 intraoperative death, and 2 others) were noticed. Postoperatively, 4 patients (23.5%) had complications; coccygodynia, back sore because of screw prominence, impingement of iliac screw, and loosening of the rod from L5 screw. All the patients were satisfied with the treatment. There were no major pulmonary complications requiring admission postoperatively. CONCLUSIONS: Although complications are associated with the treatment of paralytic NMS, a good clinical and function outcome suggests that poor PFT should not be considered as a contraindication of scoliosis surgery.


Assuntos
Atrofia Muscular Espinal/complicações , Distrofia Muscular de Duchenne/complicações , Procedimentos Neurocirúrgicos/métodos , Insuficiência Respiratória/fisiopatologia , Insuficiência Respiratória/cirurgia , Escoliose/cirurgia , Adolescente , Adulto , Criança , Feminino , Seguimentos , Humanos , Masculino , Atrofia Muscular Espinal/fisiopatologia , Distrofia Muscular de Duchenne/fisiopatologia , Procedimentos Neurocirúrgicos/instrumentação , Procedimentos Neurocirúrgicos/mortalidade , Insuficiência Respiratória/etiologia , Estudos Retrospectivos , Escoliose/etiologia , Escoliose/fisiopatologia , Fusão Vertebral/instrumentação , Fusão Vertebral/métodos , Fusão Vertebral/mortalidade , Adulto Jovem
14.
Korean J Thorac Cardiovasc Surg ; 53(2): 41-48, 2020 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-32309201

RESUMO

BACKGROUND: Chylothorax after congenital heart surgery is not an uncommon complication, and it is associated with significant morbidity. However, consensus treatment guidelines are lacking. To improve the treatment outcomes of patients with postoperative chylothorax, we implemented a standardized management protocol at Severance Hospital in September 2014. METHODS: A retrospective review of patients treated at a single center was done. All corrective and palliative operations for congenital heart disease performed at our institution between January 2008 and April 2018 were reviewed. The incidence and treatment outcomes of postoperative chylothorax were analyzed. RESULTS: The incidence of chylothorax was 1.9%. Sixty-one percent of the patients could be managed with a low-fat diet, while 28% of the patients required complete restriction of enteral feeding. Thoracic duct embolization was performed in 2 patients and chest tube drainage decreased immediately after the procedure. No patient required thoracic duct ligation or pleurodesis. After implementation of the institutional management protocol, the number of chest tube drainage days decreased (median, 24 vs. 14 days; p=0.45). CONCLUSION: Implementing a strategy to reduce postoperative chylothorax resulted in an acceptable incidence of postoperative chylothorax. Instituting a clinical practice protocol helped to curtail the treatment duration and to decrease the requirement for surgical treatment. Image-guided embolization of the thoracic duct is an effective treatment for postoperative chylothorax.

15.
Microbiol Resour Announc ; 8(45)2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31699755

RESUMO

Margaritispora aquatica is an aquatic fungal species found in leaf litter. Here, we report the 42.5-Mb draft genome sequence of M. aquatica strain NNIBRFG339, which comprises 61 scaffolds and has an overall G+C content of 45.77% and an N 50 value of 1.856 Mb.

16.
Korean J Thorac Cardiovasc Surg ; 52(4): 232-235, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31403029

RESUMO

A 2.5-kg neonate with coarctation of the aorta and a small left ventricle experienced a severe pulmonary hypertensive crisis. An emergency pulmonary artery-to-systemic artery shunt was placed to break the positive feedback loop caused by pulmonary hypertension and functional mitral stenosis. This shunt provided immediate relief of suprasystemic pulmonary hypertension and the resultant low cardiac output.

17.
Sci Rep ; 9(1): 12457, 2019 08 28.
Artigo em Inglês | MEDLINE | ID: mdl-31462655

RESUMO

Recently, bacterial endophytes (BEs) have gained importance in the agricultural sector for their use as biocontrol agents to manage plant pathogens. Outbreak of the pine wilt disease (PWD) in Korea has led researchers to test the feasibility of BEs in controlling the pine wood nematode (PWN) Bursaphelenchus xylophilus. In this study, we have reported the diversity and biocontrol activity of BEs against the PWN. By employing a culture-dependent approach, 1,622 BEs were isolated from the needle, stem, and root tissues of P. densiflora, P. rigida, P. thunbergii, and P. koraiensis across 18 sampling sites in Korea. We classified 389 members based on 16S rDNA analysis and taxonomic binning, of which, 215 operational taxonomic units (OTUs) were determined. Using Shannon's indices, diversity across the Pinus species and tissues was estimated to reveal the composition of BEs and their tissue-specific preferences. When their ethyl acetate crude extracts were analysed for biocontrol activity, 44 candidates with nematicidal activity were obtained. Among these, Stenotrophomonas and Bacillus sp. exhibited significant inhibitory activity against PWN during their developmental stages. Altogether, our study furnishes a basic comprehension of bacterial communities found in the Pinus species and highlights the potential of BEs as biocontrol agents to combat PWD.


Assuntos
Antinematódeos , Bacillus , Nematoides/crescimento & desenvolvimento , Controle Biológico de Vetores , Pinus , Stenotrophomonas , Animais , Antinematódeos/metabolismo , Antinematódeos/farmacologia , Bacillus/classificação , Bacillus/genética , Bacillus/metabolismo , Pinus/microbiologia , Pinus/parasitologia , Stenotrophomonas/classificação , Stenotrophomonas/genética , Stenotrophomonas/isolamento & purificação , Stenotrophomonas/metabolismo
18.
J Ginseng Res ; 43(3): 408-420, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31308813

RESUMO

BACKGROUND: Ginseng (Panax ginseng Meyer) is an invaluable medicinal plant containing various bioactive metabolites (e.g., ginsenosides). Owing to its long cultivation period, ginseng is vulnerable to various biotic constraints. Biological control using endophytes is an important alternative to chemical control. METHODS: In this study, endophytic Trichoderma citrinoviride PG87, isolated from mountain-cultivated ginseng, was evaluated for biocontrol activity against six major ginseng pathogens. T. citrinoviride exhibited antagonistic activity with mycoparasitism against all ginseng pathogens, with high endo-1,4-ß-D-glucanase activity. RESULTS: T. citrinoviride inoculation significantly reduced the disease symptoms caused by Botrytis cinerea and Cylindrocarpon destructans and induced ginsenoside biosynthesis in ginseng plants. T. citrinoviride was formulated as dustable powder and granules. The formulated agents also exhibited significant biocontrol activity and induced ginsenosides production in the controlled environment and mountain area. CONCLUSION: Our results revealed that T. citrinoviride has great potential as a biological control agent and elicitor of ginsenoside production.

19.
Sci Rep ; 8(1): 4302, 2018 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-29511292

RESUMO

A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper.

20.
Yonsei Med J ; 48(4): 639-44, 2007 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-17722236

RESUMO

PURPOSE: Homograft benefits include excellent hemodynamics, resistance to infection, decreased thromboembolic events, ease of handling, and lack of need for anticoagulation. We examined the short and mid-term results of right ventricular outflow tract (RVOT) reconstruction using cryopreserved homografts. PATIENTS AND METHODS: From May 1998 to May 2005, 20 patients (male:female=10:10) underwent RVOT reconstruction using cryopreserved homografts. The median age was 23.8 years (range, 0.9 to 43.3 years) and the median body weight was 57 kg (range, 7.3 to 80 kg). Eighteen patients underwent re-operation after shunt or corrective operations. Homograft failure was defined as either re-operation for homograft replacement or patient death. Homograft dysfunction was defined as grade 3 or more than 3 of graft regurgitation and more than 40 mmHg of transvalvular pressure gradient under echocardiographic examination. RESULTS: No operative mortality occurred and there were three major complications. Graft failure was observed in one male patient with tetralogy of Fallot. The 8-year freedom from graft failure was 87.5+/-11.7% and the 7-year freedom from graft dysfunction was 62.3+/-17.9%. Multivariable analysis revealed that the independent factor for graft dysfunction was age less than 10 years. In the analysis according to age group, the 7-year freedom from graft dysfunction in the group of patients older than 10 years was 100% and 25.0+/-21.7% in patients age 10 or younger (p= 0.03). CONCLUSION: Right ventricular outflow reconstruction using cryopreserved homografts provided excellent short and mid-term results in most patients in this study. However, in patients younger than 10 years old, homografts for RVOT reconstruction showed a high dysfunction rate at mid-term.


Assuntos
Cardiopatias/cirurgia , Ventrículos do Coração/transplante , Adolescente , Adulto , Criança , Pré-Escolar , Criopreservação , Feminino , Humanos , Lactente , Complicações Intraoperatórias , Masculino , Complicações Pós-Operatórias , Transplante Homólogo/efeitos adversos , Resultado do Tratamento
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