RESUMO
Direct and by immunofluorescence examination of smears, conducted on 682 women with different diseases of the cervix uteri, allowed the detection of Chl. trachomatis in 10.5% and 11.1% of the subjects, respectively. The incidence of the infection was significantly higher among unmarried women (33.7%) than among married ones (4.2%). In a second group of 305 women, sexual partners of men with chlamydial urethritis, the incidence of infection as found by the two methods of diagnosis was 57.3% and 59.3%, respectively. Colposcopy showed that more than 90% of these women had lesions at the cervix uteri level. High frequency of cervicitis, associated frequently with ectopy of the cervical epithelium in the case of Chl. trachomatis infection, suggested the determinant implication of this pathogen in the inflammatory lesions of the cervix uteri. The ectopy of the cervix epithelium is probably a factor favoring the multiplication of the pathogen.
Assuntos
Infecções por Chlamydia/diagnóstico , Chlamydia trachomatis , Doenças do Colo do Útero/diagnóstico , Adolescente , Adulto , Infecções por Chlamydia/epidemiologia , Infecções por Chlamydia/microbiologia , Chlamydia trachomatis/imunologia , Chlamydia trachomatis/isolamento & purificação , Feminino , Imunofluorescência , Humanos , Incidência , Ciclo Menstrual , Romênia/epidemiologia , Doenças do Colo do Útero/epidemiologia , Doenças do Colo do Útero/microbiologia , Esfregaço VaginalRESUMO
A vaccine against chlamydial ovine abortion was prepared according to an original method and experimentally applied to more than 500,000 animals in large breeding units. The vaccine induced a considerable seroconversion (as demonstrated by complement fixation tests) and reduced by 3--5 times the damage due to chlamydioses. Vaccination of primiparous ewes, followed by 2--3 annual revaccinations, gave good results, conferring resistance on the vaccinated animals.
Assuntos
Aborto Séptico/veterinária , Aborto Animal/prevenção & controle , Infecções por Chlamydia/veterinária , Doenças dos Ovinos/prevenção & controle , Vacinação/veterinária , Aborto Séptico/prevenção & controle , Animais , Vacinas Bacterianas , Chlamydia/imunologia , Infecções por Chlamydia/prevenção & controle , Feminino , Gravidez , OvinosRESUMO
An outbreak of 111 cases of acute respiratory tract infection was recorded in a community of the town "T" in April-May 1984. The clinical picture was severer than usual; 28% of the cases had to be hospitalized, average absenteeism being as high as 26 days per case. Serological investigations demonstrated the previous circulation of influenza virus B/Singapore/222/79 and the simultaneous circulation during the outbreak of influenza virus A/England/333/80 (H1N1) and of Rickettsia burneti (as also ascertained by isolation in the chick embryo of the former and by visualization by immunofluorescence in exfoliated cells of the latter pathogen). The association of the two etiological agents appears to account for the severe and protracted course of the disease.
Assuntos
Surtos de Doenças/epidemiologia , Influenza Humana/epidemiologia , Febre Q/epidemiologia , Infecções Respiratórias/epidemiologia , Doença Aguda , Anticorpos Antibacterianos/análise , Anticorpos Antivirais/análise , Coxiella/imunologia , Coxiella/isolamento & purificação , Humanos , Vírus da Influenza A/imunologia , Vírus da Influenza A/isolamento & purificação , Vírus da Influenza B/imunologia , Vírus da Influenza B/isolamento & purificação , Influenza Humana/imunologia , Influenza Humana/microbiologia , Nasofaringe/imunologia , Nasofaringe/microbiologia , Febre Q/imunologia , Febre Q/microbiologia , Infecções Respiratórias/imunologia , Infecções Respiratórias/microbiologia , RomêniaRESUMO
Serological investigations were performed in order to establish the antigenic relationships between three Chlamydia strains: T44(subgroup A), T13 and Q18 (subgroup B). By using monospecific mouse immune sera a certain degree of antigenical relationships was made evident and the strains could be typed by microagglutination and microimmlnofluorescence reactions. Similar results were obtained by seroneutralization with hyperimmune sera prepared in the rooster. Complement fixation proved to be a group-specific reaction unable to reveal significant differences between the strains studied, not even with monospecific mouse sera. The antigenic relationships established between strains T44 (trachoma) and T13 (psittacosis) suggest the existence features common to subgroup A and B Chlamudia strains. The importance of typing Chlamudia strains with a view to specific prophylaxis is emphasized.
Assuntos
Antígenos de Bactérias , Chlamydia/imunologia , Animais , Antígenos de Bactérias/análise , Aves , Chlamydia/classificação , Humanos , Mamíferos , Camundongos , CoelhosRESUMO
The authors establish the optimal conditions (protein concentration, temperature) for the coupling of purified mycoplasma antigen to sheep red blood cells, in order to obtain an antigenic complex stable for at least 1 year. This comples allows specific determination by passive hemagglutination--a simple and rapid technique--of anti-mycoplasma antibodies in the sera of men or animals suspected of mycoplasma infections. Under the stated experimental conditions, antibody titers equal to or higher than 1/128 indicate an infection with M. pneumoniae.
Assuntos
Antígenos de Bactérias , Testes de Hemaglutinação , Infecções por Mycoplasma/diagnóstico , Animais , Anticorpos Antibacterianos/análise , Humanos , MycoplasmaRESUMO
Direct examination of urethral smears made evident the presence of chlamydial infection in 162 (38.1%) of 425 male patients with nongonococcal urethritis. Serum antibodies to the group antigen could be detected by complement fixation and microagglutination reactions only in about 50% of the cases with chlamydial infection. The proportion of chlamydial strains isolated in chick embryos was rather low (14.8%) and the isolation rate appeared to correlate with the abundance of urethral secretion.
Assuntos
Linfogranuloma Venéreo/epidemiologia , Uretrite/etiologia , Testes de Aglutinação , Anticorpos Antibacterianos/análise , Chlamydia trachomatis/imunologia , Chlamydia trachomatis/isolamento & purificação , Testes de Fixação de Complemento , Humanos , Linfogranuloma Venéreo/diagnóstico , Masculino , Uretra/metabolismo , Uretrite/diagnósticoRESUMO
This work presents the results of a study on some viral, chlamydial, rickettsial and mycoplasmal agents incidence and their role on several gynecological ailments: vulvovaginitis, cervicitis, cervicovaginitis. The presence of some viral, chlamydial, rickettsial and mycoplasmal antigens in the vaginal secretion was investigated by immunofluorescence technique, isolation on selective media for Mycoplasma, and MGG staining in a group of 60 women with gynecopathies (group A) and in a control group (also 60 clinically healthy women) (group B). This study pointed out to higher incidence of chlamydial and mycoplasma antigens as well as of parainfluenza virus types I and III, adenovirus and herpes virus type II in group A. The serological screening revealed significant differences between the specific antibodies to antigens found in vaginal secretions to prove the presence of an immunological response on the same category of subjects.
Assuntos
Infecções por Chlamydia/complicações , Doenças dos Genitais Femininos/etiologia , Infecções por Mycoplasma/complicações , Infecções por Rickettsia/complicações , Viroses/complicações , Anticorpos Antibacterianos/análise , Anticorpos Antivirais/análise , Chlamydia/imunologia , Chlamydia/isolamento & purificação , Infecções por Chlamydia/diagnóstico , Feminino , Doenças dos Genitais Femininos/diagnóstico , Humanos , Mycoplasma/imunologia , Mycoplasma/isolamento & purificação , Infecções por Mycoplasma/diagnóstico , Rickettsia/imunologia , Rickettsia/isolamento & purificação , Infecções por Rickettsia/diagnóstico , Cervicite Uterina/diagnóstico , Cervicite Uterina/etiologia , Vagina/imunologia , Vagina/microbiologia , Viroses/diagnóstico , Vírus/imunologia , Vírus/isolamento & purificação , Vulvovaginite/diagnóstico , Vulvovaginite/etiologiaRESUMO
In order to evaluate the efficiency of passive hemagglutination (PHA) and complement fixation (CF) tests for the diagnosis of M. pneumoniae infections, 144 serum samples--out of which 81 from patients with respiratory and cutaneous infections and 63 from subjects with other diseases (controls)--were comparatively investigated by the two methods. The PHA test made evident a positivity index of 65% in the case of mixed pneumonias, as against 17% in controls, while the results obtained by CF were almost identical in the two groups investigated. The high specificity and sensitivity of the PHA reaction recommend this method for the diagnosis of acute M. pneumoniae infections.
Assuntos
Testes de Hemaglutinação , Infecções por Mycoplasma/diagnóstico , Anticorpos Antibacterianos/análise , Testes de Fixação de Complemento , Humanos , Mycoplasma/isolamento & purificaçãoRESUMO
Viral and inframicrobial antigens were detected by immunofluorescence (IF) in exfoliated conjunctival and corneal cells from 22 patients with uveitis, 2-9 different antigens being simultaneously visualized in 17 of the cases. The treatment applied on the ground of the etiological diagnosis specified by IF reactions consisted in the administration of tetracycline, Flumidin (Virustat), standard gamma-globulin or specific antiherpes immunoglobulin, the NIVGRIP inactivated influenza vaccine. This therapy led to persistent recovery (coincident with the disappearance of the antigens from conjunctival cells) in 15 cases and to clinical improvement in 7 cases. The importance of the rapid viral diagnosis by IF for the choice of an adequate therapy of uveitis is discussed.
Assuntos
Uveíte/microbiologia , Adulto , Antígenos Virais/análise , Feminino , Imunofluorescência , Humanos , Masculino , Pessoa de Meia-Idade , Uveíte/diagnóstico , Uveíte/terapiaRESUMO
Complement fixation (CF) and passive hemagglutination (PHA) tests (the latter with a M. pneumoniae antigen coupled by glutaraldehyde onto red blood cells) were performed in 263 patients with various infectious diseases (mostly in the 1st and 2nd week after onset) and non-infectious ones. CF reaction proved to be inappropriate for the early etiological diagnosis of mycoplasma infections, since the high titers were distributed undifferentially among the various patient groups and many sera (38%) showed anticomplementary activity. A PHA titer of at least 1/128 (preferably of 1/512) points to the presence of a M. pneumoniae infection, especially if clinical, radiological and laboratory data suggest a nonbacterial or mixed pneumonia. The diagnosis is often early enough to orientate the etiological therapy towards macrolides and tetracyclines. The PHA reaction recommended is specific, sensitive, reproducible and easy to perform.
Assuntos
Testes de Fixação de Complemento , Testes de Hemaglutinação , Infecções por Mycoplasma/diagnóstico , HumanosRESUMO
The presence of viral (herpes 1 and 2, influenza A and B, parainfluenza 1, 2, and 3, adenovirus) and inframicrobial (Chlamydia, R. burneti and mycoplasma) antigens was investigated by indirect immunofluorescence reaction in exfoliated cells from 107 patients with chronic eye diseases and 77 patients with chronic respiratory diseases. Single or associated viral or inframicrobial antigens were detected in 80% of the former and in 60% of the latter patients. The proportion of multiple infections was of about 60% in both patient groups.
Assuntos
Infecções Bacterianas/diagnóstico , Oftalmopatias/etiologia , Imunofluorescência , Infecções Respiratórias/etiologia , Viroses/diagnóstico , Infecções por Adenovirus Humanos/diagnóstico , Antígenos de Bactérias/análise , Antígenos Virais/análise , Infecções por Chlamydia/diagnóstico , Humanos , Influenza Humana/diagnóstico , Ceratite Dendrítica/diagnóstico , Infecções por Mycoplasma/diagnóstico , Infecções por Paramyxoviridae/diagnóstico , Infecções por Rickettsia/diagnósticoRESUMO
The presence of 12 viral (herpes 1 and 2, influenza A(H1N1), A(H3N2) and B, parainfluenza 1, 2, 3, adenovirus 5) and inframicrobial (Chlamydia, mycoplasma, Rickettsia burneti) antigens was investigated by direct or indirect immunofluorescence (IF) reactions in exfoliated conjunctival cells from 110 patients with nonbacterial keratitis and keratoconjunctivitis. A rapid etiological diagnosis could be obtained in 101 (92%) of the cases, parainfluenza and herpes antigens being the most frequently detected. In most of the cases the simultaneous presence of several antigens was made evident. Encouraging results were obtained by the application of a specific treatment based on the diagnosis provided by the IF reaction.
Assuntos
Ceratite/diagnóstico , Ceratoconjuntivite/diagnóstico , Viroses/diagnóstico , Antígenos Virais/análise , Doença Crônica , Imunofluorescência , Humanos , Ceratite/etiologia , Ceratoconjuntivite/etiologia , Recidiva , Viroses/complicaçõesRESUMO
A high proportion of parainfluenza, herpes, adenovirus and chlamydial antigens (10.7-36%) was detected by indirect immunofluorescence in cells exfoliated in the vagina of women with genital neoplasia and with uterine cervix ectopia and dysplasia. Much lower proportions of the same antigens were found in patients with common gynecopathies or recurrent genital herpes and in pregnant women. The possible relationships between chronic virus infections and genital neoplasia in women are discussed.