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1.
Nat Med ; 1(1): 84-8, 1995 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-7584960

RESUMO

ATP-induced tumour growth inhibition is accompanied by a selective decrease in the content of the tripeptide glutathione (GSH) within the cancer cells in vivo. Depletion of cellular GSH sensitizes tumours to chemotherapy and radiation, but the usefulness of this depletion depends on whether the levels of GSH can be reduced in the tumour relative to normal tissues. We report here that administration of ATP in combination with diethylmaleate and X-rays leads to complete regression of 95% of Ehrlich ascites tumours in mice. This shows that an aggressive tumour can be eliminated by using a therapy based on modulation of GSH levels in cancer cells.


Assuntos
Trifosfato de Adenosina/uso terapêutico , Carcinoma de Ehrlich/terapia , Glutationa/antagonistas & inibidores , Animais , Antineoplásicos/uso terapêutico , Butionina Sulfoximina , Carcinoma de Ehrlich/tratamento farmacológico , Carcinoma de Ehrlich/radioterapia , Divisão Celular/efeitos dos fármacos , Terapia Combinada , Inibidores Enzimáticos/uso terapêutico , Glutamato-Cisteína Ligase/antagonistas & inibidores , Concentração de Íons de Hidrogênio , Masculino , Maleatos/uso terapêutico , Metionina Sulfoximina/análogos & derivados , Metionina Sulfoximina/uso terapêutico , Camundongos , Radiossensibilizantes/uso terapêutico , Raios X
2.
Cell Prolif ; 27(2): 115-21, 1994 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10465031

RESUMO

The origin and evolution of binucleate cells in cultures of HEp-2 cells have been studied by means of interval photography and time-lapse video-recording. Binucleate cells most frequently formed by the fusion of two sister cells born in a previous mitosis. The study of binucleate cells has shown that they are a cellular type able to successfully undergo mitosis. However, the mitosis may be bipolar, tripolar or multipolar. The daughter cells arising from these divisions do not follow a clear pattern in the number of nuclei they have, instead showing a wide range of possibilities.


Assuntos
Carcinoma/patologia , Núcleo Celular/patologia , Neoplasias Laríngeas/patologia , Fusão Celular , Humanos , Mitose , Células Tumorais Cultivadas
3.
Free Radic Biol Med ; 22(7): 1203-9, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-9098094

RESUMO

The effect of x-rays on GSH and GSSG levels in blood was studied in mice and humans. An HPLC method that we recently developed was applied to accurately determine GSSG levels in blood. The glutathione redox status (GSH/GSSG) decreases after irradiation. This effect is mainly due to an increase in GSSG levels. Mice received single fraction radiotherapy, at total doses of 1.0 to 7.0 Gy. Changes in GSSG in mouse blood can be detected 10 min after irradiation and last for 6 h within a range of 2.0-7.0 Gy. The highest levels of GSSG (20.1 +/- 2.9 microM), a 4.7-fold increase as compared with controls) in mouse blood are found 2 h after radiation exposure (5 Gy). Breast and lung cancer patients received fractionated radiotherapy at total doses of 50.0 or 60.0 Gy, respectively. GSH/GSSG also decreases in humans in a dose-response fashion. Two reasons may explain the radiation-induced increase in blood GSSG: (a) the reaction of GSH with radiation-induced free radicals resulting in the formation of thyl radicals that react to produce GSSG; and (b) an increase of GSSG release from different organs (e.g., the liver) into the blood. Our results indicate that the glutathione redox ratio in blood can be used as an index of radiation-induced oxidative stress.


Assuntos
Glutationa/análogos & derivados , Glutationa/sangue , Estresse Oxidativo/efeitos da radiação , Lesões por Radiação , Animais , Neoplasias da Mama/radioterapia , Cromatografia Líquida de Alta Pressão , Relação Dose-Resposta à Radiação , Feminino , Glucosefosfato Desidrogenase/sangue , Dissulfeto de Glutationa , Glutationa Peroxidase/sangue , Glutationa Redutase/sangue , Glutationa Transferase/sangue , Humanos , Neoplasias Pulmonares/radioterapia , Masculino , Camundongos , Oxirredução
4.
Free Radic Biol Med ; 29(9): 913-23, 2000 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-11063916

RESUMO

The effect of L-glutamine (Gln) on mitochondrial glutathione (mtGSH) levels in tumor cells was studied in vivo in Ehrlich ascites tumor (EAT)-bearing mice. Tumor growth was similar in mice fed a Gln-enriched diet (GED; where 30% of the total dietary nitrogen was from Gln) or a nutritionally complete elemental diet (SD). As compared with non-tumor-bearing mice, tumor growth caused a decrease of blood Gln levels in mice fed an SD but not in those fed a GED. Tumor cells in mice fed a GED showed higher glutaminase and lower Gln synthetase activities than did cells isolated from mice fed an SD. Cytosolic glutamate concentration was 2-fold higher in tumor cells from mice fed a GED ( approximately 4 mM) than in those fed an SD. This increase in glutamate content inhibited GSH uptake by tumor mitochondria and led to a selective depletion of mitochondrial GSH (mtGSH) content (not found in mitochondria of normal cells such as lymphocytes or hepatocytes) to approximately 57% of the level found in tumor mitochondria of mice fed an SD. In tumor cells of mice fed a GED, 6-diazo-5-norleucine- or L-glutamate-gamma-hydrazine-induced inhibition of glutaminase activity decreased cytosolic glutamate content and restored GSH uptake by mitochondria to the rate found in EAT cells of mice fed an SD. The partial loss of mtGSH elicited by Gln did not affect generation of reactive oxygen intermediates (ROIs) or mitochondrial functions (e.g., intracellular peroxide levels, O(2)(-)(*) generation, mitochondrial membrane potential, mitochondrial size, adenosine triphosphate and adenosine diphosphate contents, and oxygen consumption were found similar in tumor cells isolated from mice fed an SD or a GED); however, mitochondrial production ROIs upon TNF-alpha stimulation was increased. Our results demonstrate that glutamate derived from glutamine promotes an inhibition of GSH transport into mitochondria, which may render tumor cells more susceptible to oxidative stress-induced mediators.


Assuntos
Carcinoma de Ehrlich/metabolismo , Glutamina/farmacologia , Glutationa/metabolismo , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Trifosfato de Adenosina/metabolismo , Aminoácidos/farmacologia , Animais , Ânions/farmacologia , Carcinoma de Ehrlich/tratamento farmacológico , Carcinoma de Ehrlich/patologia , Radicais Livres/metabolismo , Humanos , Concentração de Íons de Hidrogênio , Masculino , Camundongos , Estresse Oxidativo/efeitos dos fármacos , Proteínas Recombinantes/farmacologia , Fator de Necrose Tumoral alfa/farmacologia
5.
Free Radic Biol Med ; 31(5): 642-50, 2001 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-11522449

RESUMO

L-glutamine (Gln) sensitizes tumor cells to tumor necrosis factor (TNF)-alpha-induced cytotoxicity. The type and mechanism of cell death induced by TNF-alpha was studied in Ehrlich ascites tumor (EAT)-bearing mice fed a Gln-enriched diet (GED; where 30% of the total dietary nitrogen was from Gln). A high rate of Gln oxidation promotes a selective depletion of mitochondrial glutathione (mtGSH) content to approximately 58% of the level found in tumor mitochondria of mice fed a nutritionally complete elemental diet (standard diet, SD). The mechanism of mtGSH depletion involves a glutamate-induced inhibition of GSH transport from the cytosol into mitochondria. The increase in reactive oxygen intermediates (ROIs) production induced by TNF-alpha further depletes mtGSH to approximately 35% of control values, which associates with a decrease in the mitochondrial transmembrane potential (MMP), and elicits mitochondrial membrane permeabilization and release of cytochrome c. Mitochondrial membrane permeabilization was also found in intact tumor cells cultured with a Gln-enriched medium under conditions of buthionine sulfoximine (BSO)-induced selective GSH synthesis inhibition. Enforced expression of the bcl-2 gene in tumor cells could not avoid the glutamine- and TNF-alpha-induced cell death under conditions of mtGSH depletion. However, addition of GSH ester, which delivers free intracellular GSH and increases mtGSH levels, preserved cell viability. These findings show that glutamine oxidation and TNF-alpha, by causing a change in the glutathione redox status within tumor mitochondria, activates the molecular mechanism of apoptotic cell death.


Assuntos
Apoptose/efeitos dos fármacos , Glutamina/farmacologia , Mitocôndrias/efeitos dos fármacos , Células Tumorais Cultivadas/efeitos dos fármacos , Fator de Necrose Tumoral alfa/farmacologia , Trifosfato de Adenosina/metabolismo , Animais , Caspase 3 , Caspases/metabolismo , Divisão Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Grupo dos Citocromos c/metabolismo , Dieta , Sinergismo Farmacológico , Radicais Livres/metabolismo , Glutationa/metabolismo , Peróxido de Hidrogênio , Masculino , Potenciais da Membrana , Camundongos , Mitocôndrias/metabolismo , Necrose , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Oxigênio/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Superóxidos , Células Tumorais Cultivadas/patologia
6.
Cancer Lett ; 39(2): 179-83, 1988 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-3359413

RESUMO

The incorporation of cis-diammine Dichloro Platinum (II) (cisplatin) on the Ehrlich ascites carcinoma (EAT) cells has been studied in this paper. Ultrastructural study of cells treated 'in vivo' with cisplatin showed that a new treatment with this substance after fixation, blocks uranyl acetate staining with the consequent lack of heterochromatin contrast.


Assuntos
Carcinoma de Ehrlich/análise , Cisplatino/análise , Animais , Carcinoma de Ehrlich/ultraestrutura , Núcleo Celular/análise , DNA/análise , Feminino , Camundongos , Compostos Organometálicos , Distribuição Tecidual
7.
Curr Pharm Biotechnol ; 2(2): 119-30, 2001 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-11480417

RESUMO

TNF is a macrophage/monocyte-derived cytokine with cytostatic and cytotoxic anti-tumour activity. TNF-alpha can cause haemorrhagic necrosis and regression of experimental tumours. Nevertheless, the TNF-alpha doses required to cure tumour-bearing mice lead to injury of normal tissues and, eventually, may cause a lethal shock syndrome. This toxicity implies severe limitations for the therapeutic use of TNF-alpha. Reactive oxygen intermediates (ROls) are involved in TNF-alpha-induced cell killing. Different studies are consistent with the hypothesis that tumour cell sensitivity to TNF-alpha is related to its capacity to buffer oxidative attack. Recently, we have demonstrated that the sensitivity of Ehrlich ascites tumor (EAT) cells to TNF depends on their glutathione (GSH, the most prevalent nonprotein thiol in mammalian cells) content and their rate of proliferation. This is important because tumour cell populations under active proliferative states may show higher GSH levels, and drug- and/or radiation-resistant tumours have increased cellular levels of GSH. TNF-alpha induces a shift towards oxidation in the mitochondrial glutathione (mtGSH) status, a fact that is consistent with the hypothesis that mtGSH plays a key role in scavenging TNF-induced ROIs. GSH, which is not synthesized within mitochondria but is neccessary for their normal function, needs to be taken up from the cytosol through a high affinity multicomponent transport system. In consequence, different approaches that lead to depletion of mtGSH may improve the anticancer efficacy of TNF-alpha both in vitro and in vivo. As an example, EAT-bearing mice fed a glutamine-enriched diet (GED) show a selective increase of glutamate content witihin the tumour cells. Glutamate inhibits GSH uptake by tumour mitochondria and leads to a selective depletion of mtGSH content (not found in mitochondria of normal cells) to approx. 57% of the level found in tumour mitochondria of mice fed a standard diet (SD). Administration of rhTNF-alpha, which increases generation of mitochondrial ROIs, to EAT-bearing mice fed a SD does not affect significantly the rate of tumour growth. However, when tumour-bearing mice fed a GED where treated with rhTNF-alpha the number of viable tumour cells was decreased to approx. 38% of controls.


Assuntos
Neoplasias/tratamento farmacológico , Neoplasias/patologia , Fator de Necrose Tumoral alfa/farmacologia , Fator de Necrose Tumoral alfa/uso terapêutico , Animais , Resistencia a Medicamentos Antineoplásicos , Humanos , Células Tumorais Cultivadas/efeitos dos fármacos , Células Tumorais Cultivadas/metabolismo
8.
Mutat Res ; 291(1): 35-41, 1993 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-7678913

RESUMO

Chinese hamster ovary (CHO) cells were treated with a single dose (10 micrograms/ml) of cis-diamminodichloroplatinum (II) (cisplatin) for 1 h and the effect of the drug on the kinetics of proliferation of the cultures was studied. It was found that the drug produces a delay in the proliferation rates of the treated cultures. The induction of micronuclei and binucleated cells (BC) at different times after treatment have also been studied, and the ability of these cells to undergo DNA synthesis (measured as the ability to incorporate [3H]thymidine) is shown. It was also found that cisplatin induced a particular type of BC that contains one or more micronuclei rather than a pure population of BC. The results obtained show a possible relationship between micronuclei and BC. The possibility that some of the micronucleated cells evolve in subsequent cell divisions to BC with micronuclei is suggested.


Assuntos
Cisplatino/toxicidade , Micronúcleos com Defeito Cromossômico/efeitos dos fármacos , Animais , Células CHO , Divisão Celular/efeitos dos fármacos , Cricetinae
9.
Mutat Res ; 241(2): 115-24, 1990 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-2345550

RESUMO

The mutagenicity and cytotoxicity of cis-diamminedichloroplatinum (II) (cisplatin) at doses of 5, 10 and 20 micrograms/ml in Chinese hamster ovary (CHO) cells have been examined. A morphological characterization of several cell types induced by cisplatin was carried out. The frequencies of both cells with micronuclei and binucleate cells as a time-dependent parameter have also been studied. Whilst the number of cells with micronuclei was found to decrease with time, the number of binucleate cells increased. The possible kinetic mechanism for the production of binucleate cells and cells with micronuclei is discussed. A morphometric analysis was also performed. The nuclear area in both treated and control nuclei was measured with the IBAS image analysis system. The results of this analysis show that a continuous reduction in the nuclear size in the control cells is produced. However the size of the treated cells increased after treatment.


Assuntos
Núcleo Celular/efeitos dos fármacos , Cisplatino/toxicidade , Testes para Micronúcleos , Animais , Divisão Celular/efeitos dos fármacos , Núcleo Celular/patologia , Células Cultivadas , Cricetinae , Relação Dose-Resposta a Droga , Interfase/efeitos dos fármacos
10.
Biofactors ; 8(1-2): 23-6, 1998.
Artigo em Inglês | MEDLINE | ID: mdl-9699004

RESUMO

Glutathione (GSH) and the rate of cellular proliferation determine tumour cell sensitivity to tumour necrosis factor (TNF). Buthionine sulphoximine (BSO), a selective inhibitor of GSH synthesis, inhibits tumour growth and increases recombinant human TNF (rhTNF)-alpha cytoxicity in vitro. Administration of sublethal doses of rhTNF-alpha to Ehrlich ascites-tumour (EAT)-bearing mice induces oxidative stress (as measured by increases in intracellular peroxide levels, O2.- generation and mitochondrial GSSG). ATP-induced selective GSH depletion, when combined with rhTNF-alpha administration, affords a 61% inhibition of tumour growth and results in a significant extent of host survival. Administration of N-acetylcysteine (NAC) or GSH ester abolishes the rhTNF-alpha and ATP-induced effects on tumour growth by maintaining high GSH levels in the cancer cells. TNF-induced mitochondria GSH depletion appears critical in the cascade of events that lead to cell death.


Assuntos
Carcinoma de Ehrlich/patologia , Glutationa/metabolismo , Estresse Oxidativo/fisiologia , Fator de Necrose Tumoral alfa/toxicidade , Animais , Carcinoma de Ehrlich/fisiopatologia , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Dissulfeto de Glutationa/metabolismo , Humanos , Camundongos , Estresse Oxidativo/efeitos dos fármacos , Proteínas Recombinantes/toxicidade , Superóxidos/metabolismo
11.
J Agric Food Chem ; 60(42): 10641-8, 2012 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-23013444

RESUMO

Soluble and membrane-bound peroxidases (PODs) were extracted from red cabbage using Triton X-114. Optimum activity was obtained at pH 4.0 for both enzymes, and both were inactivated by sodium dodecyl sulfate (SDS). The K(M) and V(m) values for H(2)O(2) were found to be 0.98 mM and 8.1 µM/min, respectively, for soluble POD and 0.82 mM and 6.1 µM/min, respectively, for membrane-bound POD. When the 2,2'-azinobis(3-ethylbenzothiazolinesulfonic acid (ABTS) concentration was increased, maintaining a steady concentration of H(2)O(2), the activity was inhibited at the highest ABTS concentrations in soluble POD. Ascorbic acid was found to be the most active modulator of POD activity. The effect of cyclodextrins was also studied, and the complexation constant between ABTS and hydroxypropyl-ß-cyclodextrins (HP-ß-CDs) was calculated (K(c) = 312 M(-1)). Membrane-bound POD is more thermostable than soluble POD, losing >90% of relative activity after 5 min of incubation at 76.6 and 30.2 °C, respectively.


Assuntos
Brassica/metabolismo , Peroxidases/metabolismo , Brassica/enzimologia , Estabilidade Enzimática , Temperatura Alta , Concentração de Íons de Hidrogênio , Cinética
12.
Biol Cell ; 60(3): 255-7, 1987.
Artigo em Inglês | MEDLINE | ID: mdl-2962683

RESUMO

Time-dependent frequency distribution of binucleate cells (BC) was studied in Ehrlich ascites tumor (EAT) growing in mice. In animals that received no further treatment, the number of BC increased slowly from 2.6% to 16.5% of total cells within 8 days. In animals that were treated with different doses of 5-fluorouracil (FU) we found clearly higher numbers of BC. The number of BC increased with tumor age. The increase observed after treatment was reached more quickly in animals that had received the highest FU dose. The final number of BC was also dependent on the age of the tumor at the time of FU injection.


Assuntos
Carcinoma de Ehrlich/patologia , Núcleo Celular/ultraestrutura , Fluoruracila/farmacologia , Animais , Relação Dose-Resposta a Droga , Feminino , Camundongos , Índice Mitótico
13.
Biol Cell ; 65(1): 75-7, 1989.
Artigo em Inglês | MEDLINE | ID: mdl-2706394

RESUMO

An autoradiographic study was performed on binucleate and mitotic cells in the Ehrlich ascites tumor (EAT) untreated and after treatment with 5-fluorouracil (FU). The number of binucleate cells was greater in the treated tumor than in the controls. It was also observed that the number of labeled mitoses was greater in the Fu-treated tumor. Autoradiographic labeling showed that the cells that proved to be binucleate had previously passed through S-phase; thus, these cells belonged to the proliferative compartment.


Assuntos
Carcinoma de Ehrlich/ultraestrutura , Núcleo Celular/ultraestrutura , Animais , Autorradiografia , Carcinoma de Ehrlich/patologia , Núcleo Celular/efeitos dos fármacos , Feminino , Fluoruracila/farmacologia , Camundongos , Camundongos Endogâmicos , Índice Mitótico , Timidina/metabolismo , Trítio
14.
Biochem J ; 325 ( Pt 1): 183-9, 1997 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-9224645

RESUMO

Low rates of cellular proliferation are associated with low GSH content and enhanced sensitivity of Ehrlich ascites-tumour (EAT) cells to the cytotoxic effects of recombinant human tumour necrosis factor (rhTNF-alpha). Buthionine sulphoximine, a selective inhibitor of GSH synthesis, inhibited tumour growth and increased rhTNF-alpha cytoxicity in vitro. Administration of sublethal doses (10(6)units/kg per day) of rhTNF-alpha to EAT-bearing mice promoted oxidative stress (as measured by increases in intracellular peroxide levels, O2(-); generation and mitochondrial GSSG) and resulted in a slight reduction (19%) in tumour cell number when controls showed the highest rate of cellular proliferation. ATP (1mmol/kg per day)-induced selective GSH depletion, when combined with rhTNF-alpha administration, afforded a 61% inhibition of tumour growth and resulted in a significant extension of host survival. Administration of N-acetylcysteine (1mmol/kg per day) or GSH ester (5mmol/kg per day) abolished the rhTNF-alpha- and ATP-induced effects on tumour growth by maintaining high GSH levels in the cancer cells. Our results demonstrate that the sensitivity of tumour cells to rhTNF-alpha in vivo depends on their GSH content and their rate of proliferation.


Assuntos
Butionina Sulfoximina/farmacologia , Carcinoma de Ehrlich/patologia , Glutationa/metabolismo , Fator de Necrose Tumoral alfa/farmacologia , Animais , Divisão Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Sinergismo Farmacológico , Glutationa/análogos & derivados , Glutationa/farmacologia , Humanos , Cinética , Masculino , Camundongos , Camundongos Endogâmicos , Proteínas Recombinantes/farmacologia , Células Tumorais Cultivadas
15.
J Biol Chem ; 276(28): 25775-82, 2001 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-11313348

RESUMO

The mechanism of NO- and H(2)O(2)-induced tumor cytotoxicity was examined during B16 melanoma (B16M) adhesion to the hepatic sinusoidal endothelium (HSE) in vitro. We used endothelial nitric-oxide synthetase gene disruption and N(G)-nitro-l-arginine methyl ester-induced inhibition of nitric-oxide synthetase activity to study the effect of HSE-derived NO on B16M cell viability. Extracellular H(2)O(2) was removed by exogenous catalase. H(2)O(2) was not cytotoxic in the absence of NO. However, NO-induced tumor cytotoxicity was increased by H(2)O(2) due to the formation of potent oxidants, likely ( small middle dot)OH and (-)OONO radicals, via a trace metal-dependent process. B16M cells cultured to low density (LD cells), with high GSH content, were more resistant to NO and H(2)O(2) than B16M cells cultured to high density (HD cells; with approximately 25% of the GSH content found in LD cells). Resistance of LD cells decreased using buthionine sulfoximine, a specific GSH synthesis inhibitor, whereas resistance increased in HD cells using GSH ester, which delivers free intracellular GSH. Because NO and H(2)O(2) were particularly cytotoxic in HD cells, we investigated the enzyme activities that degrade H(2)O(2). NO and H(2)O(2) caused an approximately 75% (LD cells) and a 60% (HD cells) decrease in catalase activity without affecting the GSH peroxidase/GSH reductase system. Therefore, B16M resistance to the HSE-induced cytotoxicity appears highly dependent on GSH and GSH peroxidase, which are both required to eliminate H(2)O(2). In agreement with this fact, ebselen, a GSH peroxidase mimic, abrogated the increase in NO toxicity induced by H(2)O(2).


Assuntos
Endotélio Vascular/metabolismo , Endotélio Vascular/patologia , Melanoma/patologia , Óxido Nítrico/metabolismo , Comunicação Celular , Morte Celular , Movimento Celular , Técnicas de Cocultura , Inibidores Enzimáticos/farmacologia , Humanos , Melanoma/metabolismo , NG-Nitroarginina Metil Éster/farmacologia , Metástase Neoplásica , Células Tumorais Cultivadas
16.
Biochem J ; 298 ( Pt 1): 99-105, 1994 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-8129737

RESUMO

We have investigated the effect of extracellular ATP on tumour-cell proliferation and GSH levels in Ehrlich-ascites-tumour-bearing mice. After daily administration of exogenous ATP (1 mmol/kg) during 7 days, we found a 56% inhibition of tumour growth, precisely when controls show the highest rates of cell proliferation and the highest levels of GSH. This effect is accompanied by a decrease in GSH content in the tumour, but not in normal tissues. The decrease in GSH concentration within the cancer cells is associated with a decrease in gamma-glutamylcysteine synthetase activity and in protein synthesis. Growth inhibition is mediated by generation of extracellular adenosine, which subsequently increases intracellular levels of ATP and decreases intracellular levels of UTP in the cancer cells. Our results suggest that inhibition of tumour growth by ATP is due to an adenosine-dependent pyrimidine starvation effect.


Assuntos
Trifosfato de Adenosina/farmacologia , Antineoplásicos/farmacologia , Carcinoma de Ehrlich/metabolismo , Glutationa/metabolismo , Adenosina/metabolismo , Adenosina/farmacologia , Difosfato de Adenosina/metabolismo , Trifosfato de Adenosina/sangue , Animais , Carcinoma de Ehrlich/patologia , Divisão Celular/efeitos dos fármacos , Eritrócitos/metabolismo , Glutationa/biossíntese , Masculino , Camundongos , Células Tumorais Cultivadas , Uridina Trifosfato/metabolismo
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