RESUMO
When attacked by insects, plants release mixtures of volatile compounds that are beneficial for direct or indirect defense. Natural variation of volatile emissions frequently occurs between and within plant species, but knowledge of the underlying molecular mechanisms is limited. We investigated intraspecific differences of volatile emissions induced from rosette leaves of 27 accessions of Arabidopsis (Arabidopsis thaliana) upon treatment with coronalon, a jasmonate mimic eliciting responses similar to those caused by insect feeding. Quantitative variation was found for the emission of the monoterpene (E)-beta-ocimene, the sesquiterpene (E,E)-alpha-farnesene, the irregular homoterpene 4,8,12-trimethyltridecatetra-1,3,7,11-ene, and the benzenoid compound methyl salicylate. Differences in the relative emissions of (E)-beta-ocimene and (E,E)-alpha-farnesene from accession Wassilewskija (Ws), a high-(E)-beta-ocimene emitter, and accession Columbia (Col-0), a trace-(E)-beta-ocimene emitter, were attributed to allelic variation of two closely related, tandem-duplicated terpene synthase genes, TPS02 and TPS03. The Ws genome contains a functional allele of TPS02 but not of TPS03, while the opposite is the case for Col-0. Recombinant proteins of the functional Ws TPS02 and Col-0 TPS03 genes both showed (E)-beta-ocimene and (E,E)-alpha-farnesene synthase activities. However, differential subcellular compartmentalization of the two enzymes in plastids and the cytosol was found to be responsible for the ecotype-specific differences in (E)-beta-ocimene/(E,E)-alpha-farnesene emission. Expression of the functional TPS02 and TPS03 alleles is induced in leaves by elicitor and insect treatment and occurs constitutively in floral tissues. Our studies show that both pseudogenization in the TPS family and subcellular segregation of functional TPS enzymes control the variation and plasticity of induced volatile emissions in wild plant species.
Assuntos
Alquil e Aril Transferases/metabolismo , Alelos , Proteínas de Arabidopsis/metabolismo , Arabidopsis/enzimologia , Comportamento Alimentar , Liases Intramoleculares/metabolismo , Complexos Multienzimáticos/metabolismo , Pirofosfatases/metabolismo , Terpenos/metabolismo , Monoterpenos Acíclicos , Alcenos/química , Alcenos/metabolismo , Alquil e Aril Transferases/química , Alquil e Aril Transferases/genética , Sequência de Aminoácidos , Animais , Arabidopsis/citologia , Arabidopsis/genética , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Sequência de Bases , Citosol/enzimologia , Eritritol/análogos & derivados , Eritritol/metabolismo , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Insetos , Liases Intramoleculares/química , Liases Intramoleculares/genética , Redes e Vias Metabólicas , Ácido Mevalônico/metabolismo , Dados de Sequência Molecular , Complexos Multienzimáticos/química , Complexos Multienzimáticos/genética , Plastídeos/enzimologia , Regiões Promotoras Genéticas/genética , Transporte Proteico , Pirofosfatases/química , Pirofosfatases/genética , Proteínas Recombinantes/metabolismo , Sesquiterpenos/química , Sesquiterpenos/metabolismo , Frações Subcelulares/enzimologia , Fosfatos Açúcares/metabolismo , VolatilizaçãoRESUMO
Traditional lectures and cookbook laboratory exercises are today's standard tools in scientific teaching and learning. However, these conventional methods are suboptimal. Combining active learning techniques with physical experiences can improve educational success significantly. Still, hands-on material which supports active and physical teaching concepts is rare. Here, we introduce an interactive, performance-based method.As an example, we studied autophagosome formation. We observed assembly of the phagophore by membrane fusion, cargo isolation by bending the phagophore and membrane scission. We extracted characteristic time scales of autophagosome formation. Moreover, we observed capturing the autophagic cargo within a single membrane for the first time. In this chapter, we provide an easy tool to engage participants in the process of scientific perception. We are convinced that "hands-on" experiments and interactive analyses will encourage students to participate more actively in classes and thus, will improve learning. Moreover, we anticipate that the approach enhances translation of scientific concepts between different fields by providing scientists with a fresh view on, e.g., membrane-bound processes and can improve communication of science to the public.
Assuntos
Autofagossomos/metabolismo , Autofagia/fisiologia , Biologia/educação , Treinamento por Simulação/métodos , Currículo , Humanos , Membranas Intracelulares/metabolismo , Fusão de Membrana/fisiologia , Estudantes , Tóquio , Gravação em Vídeo/instrumentação , Gravação em Vídeo/métodosRESUMO
The evolution of novel morphological features, such as feathers, involves the modification of developmental processes regulated by gene networks. The fact that genetic novelty operates within developmental constraints is the central tenet of the 'evo-devo' conceptual framework. It is supported by findings that certain molecular regulatory pathways act in a similar manner in the development of morphological adaptations, which are not directly related by common ancestry but evolved convergently. The Pax6 gene, important for vision in molluscs, insects and vertebrates, and Hox genes, important for tetrapod limbs and fish fins, exemplify this 'deep homology'. Recently, 'evo-devo' has expanded to the molecular analysis of behavioural traits, including social behaviour, learning and memory. Here, we apply this approach to the evolution of human language. Human speech is a form of auditory-guided, learned vocal motor behaviour that also evolved in certain species of birds, bats and ocean mammals. Genes relevant for language, including the transcription factor FOXP2, have been identified. We review evidence that FoxP2 and its regulatory gene network shapes neural plasticity in cortico-basal ganglia circuits underlying the sensory-guided motor learning in animal models. The emerging picture can help us understand how complex cognitive traits can 'descend with modification'.
Assuntos
Evolução Biológica , Fatores de Transcrição Forkhead/metabolismo , Regulação da Expressão Gênica/fisiologia , Idioma , Fala/fisiologia , Animais , Fatores de Transcrição Forkhead/genéticaRESUMO
Despite the fact that Arabidopsis is largely self-pollinating, its flowers emit a complex mixture of terpene volatiles consisting predominantly of a large group of over 20 sesquiterpenes. Here we report that only two terpene synthases, encoded by the florally expressed genes At5g23960 and At5g44630, are responsible for the formation of virtually all sesquiterpenes found in the Arabidopsis floral volatile blend. Two independent mutant lines with T-DNA insertions in the previously identified At5g23960 gene lacked the emission of three sesquiterpenes, including the main sesquiterpene volatile (E)-beta-caryophyllene, confirming the previous in vitro functional assignment for this gene. Flowers of a mutant line carrying a T-DNA insertion in gene At5g44630 emitted these three sesquiterpenes, but did not emit any of the remaining sesquiterpene volatiles. An At5g44630 cDNA was expressed in Escherichia coli and the produced protein catalyzed the conversion of farnesyl diphosphate into over 15 sesquiterpenes in similar proportions to those found in the floral volatile blend. At5g23960 and At5g44630 promoter-beta-glucuronidase (GUS) fusion experiments demonstrated that both genes are expressed in several parts of the Arabidopsis flower, with strong At5g23960 promoter-GUS activity in the stigma and strong expression of At5g44630 in intrafloral nectaries. Given the previously reported antimicrobial activity of terpenes, their production in stigmas and nectaries may serve to inhibit microbial infection at these vulnerable sites. A survey of 37 Arabidopsis thaliana ecotypes revealed quantitative, but almost no qualitative, variations of floral monoterpene and sesquiterpene emissions suggesting that floral terpene volatiles must play some significant role in the life of the Arabidopsis plant.
Assuntos
Alquil e Aril Transferases/metabolismo , Arabidopsis/enzimologia , Flores/enzimologia , Regulação da Expressão Gênica de Plantas/fisiologia , Sesquiterpenos/metabolismo , Alquil e Aril Transferases/genética , Sequência de Aminoácidos , Arabidopsis/genética , Modelos Químicos , Dados de Sequência Molecular , Estrutura Molecular , Filogenia , Regiões Promotoras Genéticas , Homologia de Sequência de Aminoácidos , Sesquiterpenos/químicaRESUMO
Arabidopsis is emerging as a model system to study the biochemistry, biological functions, and evolution of plant terpene secondary metabolism. It was previously shown that the Arabidopsis genome contains over 30 genes potentially encoding terpene synthases (TPSs). Here we report the characterization of a monoterpene synthase encoded by two identical, closely linked genes, At3g25820 and At3g25830. Transcripts of these genes were detected almost exclusively in roots. An At3g25820/At3g25830 cDNA was expressed in Escherichia coli, and the protein thus produced was shown to catalyze the formation of 10 volatile monoterpenes from geranyl diphosphate, with 1,8-cineole predominating. This protein was therefore designated AtTPS-Cin. The purified recombinant AtTPS-Cin displayed similar biochemical properties to other known monoterpene synthases, except for a relatively low K(m) value for geranyl diphosphate of 0.2 microm. At3g25820/At3g25830 promoter activity, measured with a beta-glucuronidase (GUS) reporter gene, was primarily found in the epidermis, cortex, and stele of mature primary and lateral roots, but not in the root meristem or the elongation zone. Although the products of AtTPS-Cin were not detected by direct extraction of plant tissue, the recent report of 1,8-cineole as an Arabidopsis root volatile (Steeghs M, Bais HP, de Gouw J, Goldan P, Kuster W, Northway M, Fall R, Vivanco JM [2004] Plant Physiol 135: 47-58) suggests that the enzyme products may be released into the rhizosphere rather than accumulated. Among Arabidopsis TPSs, AtTPS-Cin is most similar to the TPS encoded by At3g25810, a closely linked gene previously shown to be exclusively expressed in flowers. At3g25810 TPS catalyzes the formation of a set of monoterpenes that is very similar to those produced by AtTPS-Cin, but its major products are myrcene and (E)-beta-ocimene, and it does not form 1,8-cineole. These data demonstrate that divergence of organ expression pattern and product specificity are ongoing processes within the Arabidopsis TPS family.