Diversity of hepatitis B and C viruses in Chile.

Although there is a low prevalence rate (around 1% of the population) of infection with hepatitis B virus (HBV) and hepatitis C virus (HCV) in Chile, little is known about the diversity and molecular characteristics of the circulating viruses. In the present study, 40 HBV and 57 HCV samples from Santiago City, Chile, were examined. The phylogenetic analysis of HBV samples showed the autochthonous genotype F as the most represented genotype in the study (67.5%), while genotypes A, B, C, and D were less frequent (7.5%, 5%, 7.5%, and 12.5%, respectively). The frequency of circulation of HBV genotypes observed is in accordance with the genetic background of the Chilean population. Most of the HCV samples tested belonged to subtype 1b (82%). The coalescent analysis conducted for both the NS5A and NS5B regions of the HCV strains showed similar population growth rates, with a most recent common ancestor estimated to date between 1893 and 1901. This result may indicate that genotype 1b strains circulating in Chile have epidemiological features similar to those described for HCV genotype 1b in Brazil and the United States. However, the most recent common ancestor for Chile is older than that reported recently for genotype 1b in Argentina.

Hepatitis B virus genetic diversity in Argentina: dissimilar genotype distribution in two different geographical regions; description of hepatitis B surface antigen variants.

BACKGROUND: The hepatitis B virus (HBV) molecular epidemiological data of Argentina are still scarce, since most of the previous analyses have been performed in the Metropolitan Region. OBJECTIVES: To deepen the current molecular and epidemiological information about the geographical distribution of HBV genotypes and subgenotypes, and to describe the hepatitis B surface antigen (HBsAg) variants circulating in Argentina. STUDY DESIGN: Eighty-eight Argentine partial HBsAg sequences from both the Northern and the Metropolitan Regions of the country were analyzed along with 67 Argentine HBV sequences existing in GenBank. RESULTS: Phylogenetic and amino acid sequence analysis grouped the 88 samples as genotypes A (14.8%), D (21.6%) and F (63.6%). In the Northern Region, 44 out of the 48 sequences analyzed (91.7%) grouped as genotype F. Differently, in the Metropolitan Region, the 40 samples grouped as genotype F (30.0%), genotype D (42.5%), and genotype A (27.5%). An elevated proportion (14.8%) of the genomes presented mutations in the major hydrophilic region (MHR). CONCLUSIONS: The different genotype distribution in both Argentine regions indicates that the epidemiological landscape of HBV infection appears to be the result of the diverse human migratory movements that have given shape to the present population. Our findings show that the prevalence of HBsAg variants is quite significant among the Argentine population.

Molecular epidemiology of hepatitis B virus in Latin America.

Hepatitis B virus (HBV) is an etiological agent of acute and chronic liver disease existing throughout the world. The high genetic variability of HBV is reflected by eight genotypes (A to H), each one with a particular geographical prevalence. The global pattern of HBV genotypes is associated with the distribution of human populations among the different continents and may reflect the patterns of human migrations. Genotypes F and H are considered indigenous to Latin America. The most prevalent genetic group of Central and South America, genotype F, is subdivided into two subtypes and five clusters associated with defined geographic areas. Genotype H has been described in Mexico and Central America. This pattern provides a tool to reconstruct the initial immigration of ancestral Amerindians from Asia and their further spread through Central and South America. Other HBV genotypes found in different Latin American countries may reflect migration from other geographical areas into the region. Genotypes A and D are the signature of the European colonization that started in the sixteenth century, including slave trade from Africa. Genotypes B and C indicate the arrival of people from Southeast Asia. The impact of HBV genotypes on the natural course of HBV infection and response to treatment has been studied recently and controversial results have been obtained. The majority of the current information concerns with genotypes B and C. In contrast, very few data are available on the Latin American HBV genotypes F and H. It has been reported that liver failure and death may be more frequent in patients infected with genotype F. More studies are needed to assess the association between H13V genotypes and clinical course of infection, especially in Latin America.