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1.
Bull Environ Contam Toxicol ; 96(1): 49-54, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26589900

RESUMO

Juveniles Rhamdia quelen fish species were exposed to diclofenac for 96 h at concentrations of 0.2, 2, and 20 µg/L. Biochemical, genetic, and hematological biomarkers were assessed in the liver, kidney, and blood in order to evaluate the toxic effects. No oxidative stress was observed in liver. In kidney the superoxide dismutase activity increased in all concentrations, suggesting an alteration in the hydrogen peroxide production, but DNA damage and lipid peroxidation were not detected. Diclofenac exposure increased the red blood cells number at concentrations of 0.2 and 2 µg/L, and monocytes and neutrophils at 2 and 20 µg/L, respectively. These results suggest that acute exposure to diclofenac, even at low concentrations, caused hematologic and renal enzymatic alterations in R. quelen.


Assuntos
Peixes-Gato/sangue , Diclofenaco/toxicidade , Rim/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Animais , Aquicultura , Biomarcadores/sangue , Dano ao DNA , Rim/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Alimentos Marinhos , Testes de Toxicidade Aguda
2.
Bull Environ Contam Toxicol ; 87(6): 603-7, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21984662

RESUMO

The study evaluated the sublethal effects of the herbicides glyphosate (Roundup) and diuron (Hexaron) and the mixture of them, used extremely in agriculture, through biomarkers in fish. The glutathione S-transferase activity increased (74%) and catalase activity decreased (37%) at the higher exposure concentration of Hexaron in comparison to the control group, suggesting an activation of this metabolism route. Membrane damage was observed at the higher exposure of Roundup and in the mixture group compared to the control group, which can be related to the nuclear alterations observed in these exposed groups. The cholinesterase activity was also inhibited (37%) in mixture group compared to the control group and no gill morphology damage was found. The results suggested a potential synergic effect in some analysed parameters.


Assuntos
Characidae/metabolismo , Água Doce/química , Herbicidas/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Biomarcadores , Catalase/metabolismo , Colinesterases/metabolismo , Diurona/toxicidade , Relação Dose-Resposta a Droga , Monitoramento Ambiental/métodos , Glutationa Transferase/metabolismo , Glicina/análogos & derivados , Glicina/toxicidade , Glifosato
3.
Emerg Microbes Infect ; 8(1): 920-933, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31237479

RESUMO

In early 2017, an outbreak caused by an unknown and supposedly viral agent in the Marilena region of southern Brazil was investigated. Since the etiological agent causing the outbreak was not identified from human samples, mosquitoes from this region were collected. Three out of 121 mosquito pools collected from the region tested positive for alphavirus in molecular tests. Next generation sequencing results revealed the presence of a novel alphavirus, tentatively named here as Caainguá virus (CAAV). DNA barcoding analyses indicated that different species of Culex are hosts for CAAV. This new virus was basal to the New World encephalitic alphaviruses in a comprehensive and robust phylogenetic approach using complete genomes. Viral particles were observed in the cytosol and inside of intracellular compartments of cells in mosquito-derived cell cultures. Despite being noninfectious in vertebrate derived cell cultures, primary culturing of CAAV in human mononuclear cells suggests monocytes and lymphocytes as CAAV targets. However, the epidemiological link of CAAV on the human outbreak should be further explored.


Assuntos
Alphavirus/isolamento & purificação , Encefalite/virologia , Adulto , Alphavirus/classificação , Alphavirus/genética , Alphavirus/fisiologia , Animais , Brasil/epidemiologia , Culicidae/fisiologia , Culicidae/virologia , Encefalite/epidemiologia , Feminino , Humanos , Linfócitos/virologia , Masculino , Monócitos/virologia , Mosquitos Vetores/fisiologia , Mosquitos Vetores/virologia , Filogenia , Adulto Jovem
4.
Environ Toxicol Pharmacol ; 53: 111-120, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28545014

RESUMO

Paracetamol is one of the most widely sold non-prescription drugs. This study aimed to evaluate the effects of the paracetamol on reproductive, biochemical, genetic, histopathological and hematogical biomarkers by waterborne exposure. Male fish of Rhamdia quelen were exposed to environmental concentrations of paracetamol (0, 0.25, 2.5µg/L) in a semi-static bioassay for 21days. Hemoglobin and hematocrit were reduced upon exposure to 0.25µg/L of paracetamol. Leukocytes and thrombocytes increased after paracetamol exposure. Paracetamol reduced testosterone levels in all exposed groups and increased estradiol levels at higher concentration. Serotonin and dopamine levels increased at exposure to 0.25µg/L. Paracetamol also caused protein carbonyls and increased SOD activity in fish exposed to 2.5µg/L and in addition led to an inhibition of EROD and GST activities in both concentrations. Hepatic genotoxicity occurred at the 0.25µg/L concentration. Hepatic tissues of exposed fish showed mild blood congestion and leucocytes infiltration. The results showed that paracetamol disrupted the hypothalamic-pituitary-gonadal axis, changed hematological parameters and caused hepatotoxicity in Rhamdia quelen. The findings suggest that this drug merits attention relative to its potential endocrine disrupter effect and hepatotoxicity, even at concentrations found in the aquatic environment.


Assuntos
Acetaminofen/toxicidade , Peixes-Gato , Disruptores Endócrinos/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Peixes-Gato/sangue , Peixes-Gato/imunologia , Peixes-Gato/metabolismo , Ensaio Cometa , Citocromo P-450 CYP1A1/metabolismo , Dano ao DNA , Estradiol/sangue , Proteínas de Peixes/metabolismo , Glutationa Transferase/metabolismo , Contagem de Leucócitos , Fígado/efeitos dos fármacos , Fígado/enzimologia , Fígado/patologia , Masculino , Superóxido Dismutase/metabolismo , Testículo/efeitos dos fármacos , Testículo/patologia , Testosterona/sangue
5.
Environ Sci Pollut Res Int ; 21(15): 9145-60, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-23397177

RESUMO

The Tubarão River rises in Santa Catarina, Brazil, and has been historically affected by coal mining activities around its springhead. To evaluate its water conditions, an investigation regarding a possible decontamination gradient associated with the increased river flow toward the estuary, as well as the influence of seasonality over this gradient was performed through a series of biomarkers (vitellogenin, comet assay, lipid peroxidation, protein carbonylation, gluthatione, gluthatione S-transferase, acetylcholinesterase, light microscopy in liver, and scanning electron microscopy in gills) and chemical analysis (polycyclic aromatic hydrocarbons (PAHs) in bile and metal analysis in sediment) in the cichlid Geophagus brasiliensis. Two collections (summer and winter) were made in four distinct sites along the river, while sediments were sampled between those seasons. As expected, the contamination linked exclusively to mining activities was not observed, possibly due to punctual inputs of contaminants. The decontamination gradient was not observed, although seasonality seemed to have a critical role in the responses of biomarkers and availability of contaminants. In the summer, the fish presented higher histopathological damages and lower concentrations of PAHs, while in the winter they showed both higher genetic damage and accumulation of PAHs. The Tubarão suffers impacts from diverse activities, representing health risks for wild and human populations.


Assuntos
Biomarcadores/análise , Ciclídeos/metabolismo , Monitoramento Ambiental/métodos , Rios/química , Poluentes Químicos da Água/análise , Animais , Brasil , Ciclídeos/crescimento & desenvolvimento , Estuários , Sedimentos Geológicos/análise , Brânquias/química , Brânquias/metabolismo , Humanos , Hidrocarbonetos Policíclicos Aromáticos/análise , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Estações do Ano , Poluentes Químicos da Água/metabolismo , Qualidade da Água
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