RESUMO
An unusual feature of the c-mos oncogene is the lack of expression in mouse tissues. Recombinant plasmids that contain the strong adenovirus late promoter and different amounts of cellular DNA 5' to c-mos(rat) were constructed and tested in transfection and transcription assays. The cellular sequences inhibit RNA accumulation in mouse but not human cells and do not inhibit in vitro transcription of the plasmid DNAs.
Assuntos
Oncogenes , RNA/metabolismo , Transcrição Gênica , Adenoviridae/genética , Animais , Sequência de Bases , Regulação da Expressão Gênica , Humanos , Camundongos , Plasmídeos , Ratos , Relação Estrutura-AtividadeRESUMO
We have employed sera from patients with autoimmune disease to characterize the nuclear SS-B/La antigen in uninfected and adenovirus-infected KB cells. A 45,000-dalton phosphorylated polypeptide was specifically precipitated with anti-SS-B sera, and the level of phosphorylation was increased after infection. The increased phosphorylation appears to occur at the same amino acid residues phosphorylated in uninfected cells and results from increased phosphorylating activity rather than from altered enzyme specificity. A competition experiment between infected and uninfected cell extracts indicates that the antigen in the infected cell binds more strongly to SS-B/La antibodies. Fragments of adenovirus-induced virus-associated RNA as well as intact molecules complex with SS-B/La antigen and are immune precipitated with autoimmune sera.
Assuntos
Adenoviridae/imunologia , Nucleoproteínas/imunologia , Fosfoproteínas/imunologia , Anticorpos Antinucleares/imunologia , Complexo Antígeno-Anticorpo , Antígenos Nucleares , Doenças Autoimunes/imunologia , Células Cultivadas , Humanos , Peso Molecular , Nucleoproteínas/isolamento & purificação , Fosfoproteínas/isolamento & purificação , RNA/imunologiaRESUMO
The fluorescence of NADH bound to phosphoglycerate dehydrogenase (3-phosphoglycerate: NAD+ oxidoreductase, EC 1.1.1.95) decreased by 42% between pH 8.5 and 7.0 Serine, an allosteric inhibitor, quenched the fluorescence of enzyme-bound NADH by 29% at pH 8.5, but not at all at pH 7.0. The kinetics of the fluorescence change which occurred when the pH of an enzyme-NADH solution was rapidly shifted from 8.5 to 7.0 was measured using stopped-flow fluorimetry. The kinetics were first order, with a rate constant of 2.83 s-1. This rate constant was similar in magnitude to the rate constants for fluorescence quenching at pH 8.5 by saturating concentrations of serine and glycine, another allosteric inhibitor (Dubrow, R. and Pizer, L.I. (1977) J. Biol. Chem. 252, 1527-1538). These results indicate that the conformation of phosphoglycerate dehydrogenase at pH 7.0 is similar to, but not identical with, the serine-induced conformation at pH 8.5.
Assuntos
Oxirredutases do Álcool , Desidrogenases de Carboidrato , Oxirredutases do Álcool/metabolismo , Regulação Alostérica , Desidrogenases de Carboidrato/antagonistas & inibidores , Desidrogenases de Carboidrato/metabolismo , Escherichia coli/enzimologia , Concentração de Íons de Hidrogênio , Cinética , NAD/metabolismo , Ligação Proteica , Conformação Proteica , Serina/farmacologia , Espectrometria de FluorescênciaRESUMO
Neonatal infection with a mutant herpes simplex type 1 virus produced hyperactivity in mice. Activity was measured throughout a 24 hour period during adulthood, and the elevation of activity occurred during the period of the day when mice are normally inactive. In a second experiment, infected mice showed deficits in learning to inhibit behavior in a passive avoidance task, but no deficit in learning a complex spatial task. Virus was detected in the brain by 5 days of age. The peak percentage of mice infected was reached at 10 days of age and declined thereafter. Mortality due to the virus declined with age at which the mouse was infected, but rates of hyperactivity were not different when injection occurred within the first 4 days of life. The viral infection produced no deficit in body weight in suckling mice. Thus we have shown that a mild neonatal virus infection can produce specific behavioral deficits.
Assuntos
Encefalopatias/microbiologia , Herpes Simples/complicações , Hipercinese/etiologia , Deficiências da Aprendizagem/etiologia , Fatores Etários , Animais , Animais Recém-Nascidos , Aprendizagem da Esquiva , Encefalopatias/complicações , Herpes Simples/fisiopatologia , CamundongosAssuntos
Leucemia/metabolismo , Leucócitos/metabolismo , Fosforilação Oxidativa , Serina/biossíntese , Isótopos de Carbono , Carcinoma , Linhagem Celular , Fibroblastos/metabolismo , Glicerolfosfato Desidrogenase/metabolismo , Células HeLa/metabolismo , Humanos , Leucemia Mieloide/enzimologia , Leucemia Mieloide/metabolismo , Leucócitos/enzimologia , Neoplasias Bucais , NAD/metabolismo , Monoéster Fosfórico Hidrolases/metabolismo , Isótopos de FósforoAssuntos
Colífagos/crescimento & desenvolvimento , DNA Viral/análise , Isótopos de Carbono , Centrifugação com Gradiente de Concentração , Nucleotídeos de Citosina , Desoxirribonucleases , Escherichia coli/enzimologia , Concentração de Íons de Hidrogênio , Cinética , Substâncias Macromoleculares/biossíntese , Metiltransferases/análise , Muramidase/análise , Isótopos de Fósforo , RNA Viral/análise , RNA Viral/biossíntese , Fatores de Tempo , Uracila/análise , Valina/análise , Proteínas Virais/biossínteseAssuntos
Escherichia coli/enzimologia , Glicerolfosfato Desidrogenase/isolamento & purificação , Cromatografia em Gel , Cromatografia por Troca Iônica , Estabilidade de Medicamentos , Glicerolfosfato Desidrogenase/metabolismo , Glicerofosfatos/farmacologia , Concentração de Íons de Hidrogênio , Cinética , Métodos , Peso Molecular , Concentração Osmolar , Especificidade da Espécie , Estreptomicina , Relação Estrutura-AtividadeAssuntos
Erros Inatos do Metabolismo dos Aminoácidos/complicações , Ataxia/etiologia , Glicina/sangue , Doenças da Medula Espinal/etiologia , Adolescente , Adulto , Erros Inatos do Metabolismo dos Aminoácidos/diagnóstico , Animais , Gatos , Células Cultivadas , Criança , Feminino , Fibroblastos/metabolismo , Glicina/metabolismo , Glicina/fisiologia , Humanos , Masculino , Serina/metabolismo , Medula Espinal/fisiologia , Doenças da Medula Espinal/diagnóstico , Transmissão Sináptica , SíndromeRESUMO
Nuclei isolated from herpes simplex virus (HSV)-infected KB cells actively synthesize HSV RNA in vitro; the RNA can be hybridized with HSV DNA or nuclear RNA from HSV-infected cells. Nascent RNA molecules labeled in vivo with 32PO4 were elongated, utilizing the nuclear system to incorporate Hg-CTP at their 3' ends, and then isolated on an affinity column. Hybridization of isolated nascent RNA molecules showed that greater than 50% of them were HSV specific and that more than 25% were self-complementary.
Assuntos
Núcleo Celular/metabolismo , RNA Viral/biossíntese , Simplexvirus/metabolismo , Carcinoma , Linhagem Celular , Sistema Livre de Células , Cromatografia de Afinidade , Humanos , Neoplasias Bucais , Hibridização de Ácido Nucleico , Simplexvirus/crescimento & desenvolvimento , Replicação ViralRESUMO
The level of phosphoglycerate dehydrogenase, the first enzyme in the biosynthetic pathway to serine and glycine, has been studied in Escherichia coli grown under different conditions. The enzyme level was not reduced by growth in a medium which contained the end products of the pathway, nor was it elevated when the growth rates was limited by the supply of serine. Elevation of phosphoglycerate dehydrogenase did not occur when charging of tRNA ser was inhibited by serine hydroxamate. However, phosphoglycerate dehydrogenase levels were subject to regulation. Elevated levels of enzyme activity were observed in merodiploids containing multiple copies of the serA gene, and lowered enzyme levels were found in cells grown on carbon sources other than glucose or when certain amino acids were present in the growth medium. The combined effect of these nutritional changes (carbon source and amino acids) reduced the level of phosphoglycerate dehydrogenase to 10 to 12% of that found in wild-type cells and to about 5% of the level in the merodiploids. By using antibody prepared against purified phosphoglycerate dehydrogenase we established that the decrease in enzyme activity reflected decreased amounts of enzyme protein. Constant intracellular concentrations of 3-phosphoglycerate and serine were found in cells with marked differences in phosphoglycerate dehydrogenase activity, indicating that end product inhibition of phosphoglycerate dehydrogenase activity, rather than the amount of the biosynthetic enzymes, is the major factor in regulating the intracellular concentration of serine.
Assuntos
Desidrogenases de Carboidrato/metabolismo , Escherichia coli/enzimologia , Serina/biossíntese , Aminoácidos/farmacologia , Escherichia coli/genética , Genes , Ácidos Glicéricos/metabolismo , Glicina/metabolismo , Cinética , Lactatos/farmacologia , Fosfoglicerato DesidrogenaseRESUMO
On the basis of experiments with mutant virus and transfection with isolated genes, the herpes simplex virus immediate-early gene product ICP4 is known to positively regulate the transcription of viral early and late genes and negatively regulate expression from its own promoter. Binding of ICP4 to DNA sequences in several viral genes has been reported, yet the significance of ICP4-DNA interaction in transcriptional activation remains unclear. We have studied this problem by using the early glycoprotein D (gD) gene, which possesses a binding site at approximately -100 relative to the RNA initiation site. We linked this promoter and various mutant constructs to the chloramphenicol acetyltransferase gene in order to measure promoter activity in transient transfections both in the presence and in the absence of an ICP4-encoding plasmid. The natural promoter was activated 3.3-fold, and a deletion construct lacking the binding site was activated minimally (1.7-fold). Constructs containing multiple tandem repeats of the binding site (three or five inserts) demonstrated higher expression in the presence of ICP4 than did the natural promoter while retaining low levels of expression when unstimulated. Gel mobility shift assays and DNase I footprinting analyses indicated that ICP4 associated with multiple binding sites. In vitro transcription from a gD promoter construct containing multiple binding sites showed increased RNA synthesis in the presence of partially purified ICP4. These data provide the first direct evidence that binding of ICP4 to a specific DNA sequence in the gD gene contributes to activation of transcription.
Assuntos
DNA Viral/genética , Regulação da Expressão Gênica , Proteínas Imediatamente Precoces , Simplexvirus/genética , Proteínas Virais/genética , Animais , Sítios de Ligação , Enzimas de Restrição do DNA , DNA Viral/metabolismo , Células HeLa , Humanos , Cinética , Plasmídeos , Regiões Promotoras Genéticas , Transcrição Gênica , Células Vero , Proteínas Virais/metabolismoRESUMO
Phospholipid synthesis has been reported to be subject to stringent control in Escherichia coli. We present evidence that demonstrates a strict correlation between guanosine tetraphosphate accumulation and inhibition of phospholipid synthesis. In vivo experiments designed to examine the pattern of phospholipid labeling with (32)P-inorganic phosphate and (32)P-sn-glycerol-3-phosphate suggest that regulation must occur at the glycerol-3-phosphate acyltransferase step. Assay of phospholipid synthesis by cell-free extracts and semipurified preparations revealed that guanosine tetraphosphate inhibits at least two enzymes specific for the biosynthetic pathway, sn-glycerol-3-phosphate acyltransferase as well as sn-glycerol-3-phosphate phosphatidyl transferase. These findings provide a biochemical basis for the stringent control of lipid synthesis as well as regulation of steady-state levels of phospholipid in growing cells.
Assuntos
Escherichia coli/metabolismo , Nucleotídeos de Guanina/metabolismo , Fosfolipídeos/biossíntese , Acetiltransferases/metabolismo , Trifosfato de Adenosina/metabolismo , Aminoácidos/metabolismo , Autorradiografia , Radioisótopos de Carbono , Sistema Livre de Células , Cromatografia em Camada Fina , Nucleotídeos de Citosina , Escherichia coli/enzimologia , Glicerofosfatos/metabolismo , Guanosina Trifosfato/metabolismo , Lipídeos/biossíntese , Fosfatos/metabolismo , Fosfatidiletanolaminas , Radioisótopos de Fósforo , Fosfotransferases/metabolismo , RNA Bacteriano/biossíntese , TrítioRESUMO
Serine hydroxamate, which inhibits the charging of seryl-transfer ribonucleic acid, reduced the synthesis of phospholipid and nucleic acids in Escherichia coli. This effect was analogous to depriving amino acid auxotrophs of their nutritional requirement and appears to be a manifestation of the stringent response shown by rel(+) strains of E. coli. Amino acid starvation (serine or methionine) alone or serine hydroxamate treatment alone results in 60 to 80% inhibition of lipid accumulation, 90% inhibition of ribonucleic acid accumulation, and an increase in guanosine tetraphosphate (ppGpp). These three effects were reversed by addition of chloramphenicol (CM). A combination of serine starvation and serine hydroxamate treatment resulted in inhibition of lipid and RNA accumulation as well as an increase in ppGpp, but the consequences of the double block were not reversed by CM. We conclude that a strong interrelationship exists among these processes and that CM acts to relax a stringent response by mechanisms other than interference with ppGpp formation. All species of phospholipid were affected by a stringent response evoked by amino acid starvation or addition of serine hydroxamate, but in all cases the synthesis of phosphatidylethanolamine was most severely inhibited. Serine hydroxamate was not incorporated into lipid but specifically caused phosphatidylserine accumulation. Serine starvation produced a dramatic alteration of the distribution of isotope incorporated into phospholipid, which resulted from the stringent response compounded with the limitation of a substrate for phosphatidylserine synthesis.
Assuntos
Escherichia coli/metabolismo , Ácidos Hidroxâmicos/farmacologia , Fosfolipídeos/biossíntese , Serina/farmacologia , Trifosfato de Adenosina/metabolismo , Cloranfenicol/farmacologia , Cromatografia em Camada Fina , Escherichia coli/efeitos dos fármacos , Ésteres/farmacologia , Nucleotídeos de Guanina/metabolismo , Guanosina Trifosfato/metabolismo , Metabolismo dos Lipídeos , Mutação , Isótopos de Fósforo , RNA/metabolismo , Fatores de Tempo , TrítioRESUMO
The regulation of several enzymes involved in one-carbon metabolism was studied in a mutant of Escherichia coli K-12 defective in S-adenosylmethionine synthetase. The mutant that was reported to have a low endogenous concentration of S-adenosylmethionine had elevated levels of N-5, 10-methylene tetrahydrofolate reductase and serine transhydroxymethylase, but the level of N-5, 10-methylene tetrahydrofolate dehydrogenase was not altered. These results suggest that S-adenosylmethionine plays a role in the regulation of one-carbon production and utilization. An enzyme system that cleaved glycine to one-carbon units was demonstrated. The enzymes responsible for the cleavage of glycine were induced by exogenous glycine but were independent of S-adenosylmethionine or purine levels in the cell.
Assuntos
Escherichia coli/metabolismo , Formaldeído/biossíntese , Glicina/metabolismo , Adenina/metabolismo , Oxirredutases do Álcool/metabolismo , Radioisótopos de Carbono , Sistema Livre de Células , Meios de Cultura , Repressão Enzimática , Escherichia coli/enzimologia , Escherichia coli/crescimento & desenvolvimento , Ligases/metabolismo , Metionina/metabolismo , Mutação , Nucleosídeos , Oxirredutases/metabolismo , S-Adenosilmetionina/metabolismo , Serina/metabolismo , Espermidina/metabolismo , Tetra-Hidrofolatos , Transferases/metabolismoRESUMO
Stopped flow spectrophotometry was used to investigate the kinetics of the transition of the phosphoglycerate dehydrogenase (3-phosphoglycerate: NAD oxidoreductase, EC 1.1.1.95) reaction from the active to the inhibited rate upon the addition of the physiological inhibitor serine. The transition was characterized by a single first order rate constant (kobs,i) which was independent of enzyme concentration. At pH 8.5, kobs,i increased in a hyperbolic manner with serine concentration from 2 to 8 s-1. The increase in kobs,i occurred at serine concentrations where the steady state inhibition was virtually complete. These results indicate that serine inhibition is an allosteric process involving a conformational change in the enzyme. A model is presented in which serine at low concentrations binds exclusively to the inhibited state of the enzyme and shifts the equilibrium toward that state; at high serine concentrations, serine binds to the active state, facilitating its conversion to the inhibited state. An alternative model, which we favor, proposes two classes of inhibitor binding sites. The kinetics of the fluorescence quenching of enzyme-bound NADH by serine (Sugimoto, E., and Pizer, L.I. (1968) J. Biol. Chem. 243, 2090-2098), measured by stopped flow fluorimetry, was also characterized by a single first order rate constant (kobs,f.q.) which was independent of enzyme concentration. At pH 8.5, kobs,f.q. ranged from 0.4 s-1 at low serine concentrations to 1.1 s-1 at high serine concentrations. These results indicate that the fluorescence quenching induced by serine is a manifestation of a structural change in the enzyme. Enzyme and excess NADH were mixed with substrate and serine in the stopped flow instrument, and enzyme-bound NADH fluorescence was monitored by exciting through the protein at 285 nm. A rapid fluorescence quenching process, which occurred within the mixing time, was followed by a slower fluorescence enhancement process which terminated in a steady state level corresponding to the quenched fluorescence of the enzyme NADH serine complex. The rapid quenching was the result of substrate binding (Dubrow, R., and Pizer, L.I. (1977) J. Biol. Chem. 252, 1539-1551). The fluorescence enhancement was characterized by a single first order rate constant whose value for a given serine concentration corresponded with Kobs,j. This data shows that the quenched state of the enzyme-NADH-complex is the state which is directly responsible for the inhibition of enzyme activity. During catalysis the quenched state is achieved from a different initial conformation, and consequently at a different rate, than in the absence of substrate. kobs,j and kobs,f.q. were also measured using glycine, another inhibitor. The ultraviolet difference spectrum between enzyme and enzyme plus serine was determined and proposed to be the result of the same structural change which is responsible for the fluorescence quenching by serine.
Assuntos
Oxirredutases do Álcool/metabolismo , Desidrogenases de Carboidrato/metabolismo , Regulação Alostérica , Escherichia coli/enzimologia , Glicina/farmacologia , Cinética , Serina/farmacologia , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta , Fatores de TempoRESUMO
The catalytic mechanism of the phosphoglycerate dehydrogenase reaction in both directions was investigated by studying: (a) pre-steady state transients in reduced coenzyme appearance or disappearance or disappearance and in protein fluorescence; (b) deuterium isotope effects on the transients and on the steady state reactions; and (c) the partial reaction between the enzyme-NADH complex and hydroxypyruvate-P. These studies led to the scheme below for the ternary complex interconversion. E1-NADH-hydroxypyruvate-P(1)equilibriumE2-NADH-hydroxypyruvate-P(2)equilibriumE3-NADH-hydroxypyruvate-P + H+(3)equilibriumE3-NAD+-3-phosphoglycerate(4)equilibriumE4-NAD+-3-phosphoglycerate Steps 1,2, and 4 are ternary complex isomerizations. Step 3 is the hydride transfer. Under steady state conditions isomerization 2 is the rate-determining step in the direction of hydroxypyruvate-P reduction at higher pH values. At lower pH values, the hydride transfer step is also partially rate-determining. The rate-determining step in the direction of 3-phosphoglycerate oxidation occurs subsequent to the hydride transfer step at higher pH values. At lower pH values the rate is determined by both isomerization 4 and the hydride transfer step. Isomerizations 1, 2, and 4 were inhibited by serine, an allosteric inhibitor, indicating that the inactive conformation of the enzyme is incapable of performing any of the steps of the ternary complex interconversion. Phosphoglycerate dehydrogenase corresponds to a V-type allosteric enzyme. When the enzyme-NADH complex was mixed with hydroxypyruvate-P at pH 8.5, a rapid quenching of enzymebound NADH fluorescence occurred. This process was studied under pseudo-first order conditions and shown to be the result of hydroxypyruvate-P binding.
Assuntos
Oxirredutases do Álcool/metabolismo , Desidrogenases de Carboidrato/metabolismo , Deutério/metabolismo , Desidrogenases de Carboidrato/isolamento & purificação , Computadores , Escherichia coli/enzimologia , Concentração de Íons de Hidrogênio , Cinética , Espectroscopia de Ressonância Magnética , Serina/farmacologia , Espectrometria de Fluorescência , Fatores de TempoRESUMO
3-Phosphoglycerate dehydrogenase (3-phosphoglycerate:NAD oxidoreductase, EC. 1.1.1.95) was purified from Bacillus subtilis by conventional methods. The final preparation was homogeneous by electrophoretic analysis and had a sedimentation constant of 6.3 S. On the basis of gel filtration data the enzyme had a molecular weight of about 166000. The plot of velocity versus phosphoglycerate concentration was biphasic while similar plots for hydroxypyruvate phosphate and NADH were the conventional hyperbolic type. The enzyme was specifically inhibited by serine. The inhibition was time dependent, requiring several minutes incubation before a constant level of inhibition was achieved. Serine inhibition was of the "mixed type" with respect to 3-phosphoglycerate and Hill plots of these data had slopes that approached 2. Desensitization of the enzyme to serine inhibition was achieved by incubation in the absence of dithiothreitol. The desensitized enzyme was different from the native enzyme in fluoresence properties, sedimentation characteristics and in the absence of the biphasic phosphoglycerate saturation curve. Evidence was obtained for the participation of sulphydryl groups in the changes in protein structure responsible for serine inhibition as well as the dehydrogenase activity of the enzyme.