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An Acad Bras Cienc ; 96(3): e20230309, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39166649

RESUMO

Brazil is renowned for its extensive plant biodiversity, with emphasis on Cymbopogon, C. citratus and C. nardus, with broad antimicrobial potential. Candidemias caused by Candida albicans are highly prevalent in immunosuppressed individuals and are associated with infections by biofilms on medical devices. The aim of this study was to evaluate the antimicrobial potential of essential oils C. citratus and C. nardus against C. albicans in planktonic and biofilm forms. Essential oils were obtained by hydrodistillation and chemical composition evaluated by GC-FID and GC-MS. The minimum inhibitory concentration was determined by the broth microdilution method and the synergy effect of essential oils and amphotericin B were evaluated by the checkerboard test. Biofilm activity was determined by the XTT assay. Cytotoxicity assays performed with VERO cells and molecular docking were performed to predict the effect of oil interaction on the SAP-5 enzyme site. The results showed activity of essential oils against planktonic cells and biofilm of C. albicans. Furthermore, the oils had a synergistic effect, and low cytotoxicity. Molecular docking showed interaction between Cadinene, Caryophyllen oxide, Germacrene D with SAP-5. The results indicate that Cymbopogon spp. studied are anti-Candida, with potential for further application in therapy against infections caused by C. albicans.


Assuntos
Antifúngicos , Biofilmes , Candida albicans , Cymbopogon , Testes de Sensibilidade Microbiana , Simulação de Acoplamento Molecular , Óleos Voláteis , Cymbopogon/química , Óleos Voláteis/farmacologia , Óleos Voláteis/química , Antifúngicos/farmacologia , Antifúngicos/química , Candida albicans/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Animais , Células Vero , Chlorocebus aethiops , Cromatografia Gasosa-Espectrometria de Massas
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