RESUMO
OBJECTIVE: To study the effects of neuronal apoptosis under the induction of ß-amyloid inflammatory supernatant. METHODS: The neurons were intervened by ß-amyloid-induced inflammatory supernatant at the concentration of Aß1-42 at 125 nmol/L. And the expressions of Bcl-2, caspase-3, PARP and neuronal apoptosis were detected. RESULTS: The caspase-3 (14.2 ± 1.8), Bcl-2 (10.6 ± 0.8) positive cells of microglia inflammatory supernatant stimulated group were significantly elevated than the control (2.2 ± 0.6, 5.0 ± 0.3, P < 0.01). Nuclear chromatin was uniform yellow-green fluorescent. And there was significant difference of neuronal apoptosis between microglia inflammatory supernatant group and Aß1-42 directly stimulated group. CONCLUSION: Neuronal apoptosis is induced by caspase-3 in ß-amyloid inflammatory supernatant. One of the important causes is chronic inflammatory process of activated microglia by Aß.