Hydrogen peroxide-induced stress acclimation in plants.

Among all reactive oxygen species (ROS), hydrogen peroxide (H2O2) takes a central role in regulating plant development and responses to the environment. The diverse role of H2O2 is achieved through its compartmentalized synthesis, temporal control exerted by the antioxidant machinery, and ability to oxidize specific residues of target proteins. Here, we examine the role of H2O2 in stress acclimation beyond the well-studied transcriptional reprogramming, modulation of plant hormonal networks and long-distance signalling waves by highlighting its global impact on the transcriptional regulation and translational machinery.

Mixing tannery effluent had fertilizing effect on growth, nutrient accumulation, and photosynthetic capacity of some cucurbitaceous vegetables: A little help from foe.

Tannery effluent contains a number of organic and inorganic elements as pollutants which reduce plant growth. To overcome shortage of water, use of diluted industrial wastewater such as tannery effluent can be a viable strategy for improving crop growth and yield. A pot experiment was conducted to determine the effects of tannery effluent and its various dilutions on physiological and biochemical characteristics of five cucurbitaceous vegetables. Tannery effluent was applied 0, 25, 50, 75 and 100% to 3-week-old plants of five cucurbitaceous vegetables (Cucurbita maxima, Luffa cylindrica, Citrullus vulgaris, Cucumis melo, and Praecitrullus fistulosus) for 4 weeks. Tannery effluent reduced the growth of all five cucrbitaceous vegetables. Diluted tannery effluent (25%) improved the growth of Cucurbita maxima, Citrullus vulgaris, and Cucumis melo. Moderately diluted (50%) did not affect the growth of Citrullus vulgaris and Cucumis melo. Toxic effects of tannery effluent were associated with high accumulation of heavy metals Cr, Cd, Mn, and Fe in leaves and roots. High accumulation of heavy metals in leaves reduced the accumulation of nutrients in leaves (N, P, K) and reduced photosynthetic pigments and photosynthetic rate. Changes in photosynthetic rates of all vegetable species due to tannery effluent were not associated with stomatal limitations (stomatal conductance, transpiration rate, internal CO2). Toxic effects of tannery effluent on plants also include changes in N-metabolism (amino acid and protein). However, extent of these adverse effects of tannery effluent on vegetables was species specific. It is suggested that Cucurbita maxima can be grown by supplying 25% tannery effluent, whereas Citrullus vulgaris and Cucumis melo can be grown with moderately diluted (50%) tannery effluent.

Acuities into tolerance mechanisms via different bioassay during Brassicaceae-Alternaria brassicicola interaction and its impact on yield.

Heavy losses by dark leaf spot disease in oilseed Brassica have incited research towards identifying sources of genetic tolerance against causal pathogen, Alternaria brassicicola. Several morpho-molecular parameters were evaluated to test the performance of field mustard and rapeseed genotypes under artificial inoculation with this pathogen. During Brassica-Alternaria interaction, physio-biochemical defense response was witnessed in tolerant genotypes. Two tolerant genotypes (one for field mustard and one for rapeseed), i.e., EC250407 and EC1494 were identified. However, necrotic lesions were more prominent in susceptible genotypes with minimum chlorophyll (chlorophyll a, chlorophyll b and total chlorophyll) and carotenoids contents. Contrary to photosynthetic pigments, increase in total soluble protein (TSP) contents was observed with disease progression in susceptible genotypes. Tolerant genotypes of field mustard and rapeseed displayed remarkable increase in the activities of redox enzyme in infected leaves with least yield loss (6.47% and 5.74%) and disease severity index (DSI) of 2.9 and 2.1, respectively. However, yield/plant showed close association with other morpho-yield parameters, photosynthetic pigments and redox enzymes (superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD)) activities except silique length and TSP. Based on the results of morpho-biochemical analyses, redox enzymes and morphological parameters; their interplay is proposed to determine the tolerance outcome of the Brassica-A. brassicicola interaction.

Lead tolerant endophyte Trametes hirsuta improved the growth and lead accumulation in the vegetative parts of Triticum aestivum L.

Rapid industrialization and increasing population are continuously adding contaminants to our environment. Among those, heavy metals are considered to be one of the serious threats to the ecosystem due to their persistent nature. Microbe assisted phytoremediation is an effective tool for metal remediation as microbes enhance the metal availability and uptake to the host plants or reduce it by binding them intracellularly or extracellularly. An endophytic fungus, Trametes hirsuta, was isolated from Chenopodium album L. plant growing in the lead (Pb) contaminated soil of an industrial area. This is the first study citing Trametes hirsuta, as a root endophyte of Chenopodium album L. This endophytic fungus was found to be tolerant to high concentration of Pb i.e., 1500 mg L-1, when tested in-vitro. Wheat (Triticum aestivum L.) seedlings were infected by Trametes hirsuta and Pb tolerance was observed. With the fungal inoculation plants cumulative growth and total chlorophyll content increased by 24% and 18%, respectively as compared to their respective non-inoculated controls at 1000 mg kg-1 Pb. Similary, 50% more Pb accumulation was measured in the shoots of fungal inoculated plants at 1500 mg kg-1 Pb as compared to control. Thus, the results of the present study suggest that mutualism with endophytic fungi can improve the survival of host plants in metal contaminated soils, additionally it can also assist the phytoextraction of heavy metals from polluted sites by increasing their uptake by the host plants.

Early maturing Bt cotton requires more potassium fertilizer under water deficiency to augment seed-cotton yield but not lint quality.

Exhaustive crops such as cotton require potassium (K) in copious amounts as compared to other crops. High yielding cultivars in cotton-wheat cropping system, have further increased its demand in cotton growing areas of Pakistan. As cotton is grown in arid and semiarid areas, therefore often prone to water deficiency. The reproductive growth particularly flowering and boll setting are highly sensitive to low soil water potentials, where enough K supply can play a vital role. In this two-year field studies, three cultivars (early, mid and late maturing) were cultivated at two K fertilizer levels 100, 200 kg K ha-1 along with control with no K fertilizer application at two irrigation levels. In first irrigation level, water was applied as per full irrigation schedule, while in water deficit irrigation water was applied at deficit irrigation schedule started after flowering till harvesting. It has been revealed that K application has impact on boll setting as well as seed cotton yield, however early and mid-maturing cultivars are more responsive to K fertilization. Furthermore, irrigation level had significant impact against K fertilization and relatively better response was observed in deficit irrigation as compared to full irrigation. Nevertheless, fiber quality parameters were unaffected by K fertilization. Considering the best benefit cost ratio under water deficiency, it is concluded that 100 kg K2O ha-1 should be applied at the time of seed bed preparation for economical seed-cotton yield of early maturing Bt cotton.

Comparative insecticidal activity of different plant materials from six common plant species against Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae).

Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) is one of the major insect pests of stored grains. Due to export legislation and zero-tolerance for live insect in trade commodities, extensive use of synthetic insecticides is in practice in order to eliminate pest infestations from the lots. Currently, the one and only acceptable chemical to be used in stored grain is phosphine but due to its excessive usage the stored grain pests are becoming resistant against it. Hence discovery of alternative compounds is much needed. In this study we have compared insecticidal efficacy of different plant materials from six commonly grown plants of Pakistan, viz. Allium sativum (Alliaceae), Zingiber officinale (Zingiberaceae), Cymbopogon citratus (Poaceae), Eucalyptus globulus (Myrtaceae), Nicotiana tabacum (Solanaceae), and Azadirachta indica (Meliaceae) against T. castaneum infesting stored wheat, rice, corn and gram pulse. Various plant parts were dried, powdered, and used as admixtures to the stored commodities in the experiments. The results have suggested that A. sativum (garlic) and Z. officinale (ginger) were more effective resulting into 15 times higher adult mortality and 4 to 5 times reduction in grain weight losses when mixed with rice grains. Similarly, A. indica when admixture with wheat checked the population growth in the resources resulting into 3.5 times less adult production compared to controls. A subsequent experiment was conducted to study the dose response of neem seed powder against the beetle pest infesting milled products. Surprisingly, better control was observed either at lowest (1% w/w) or the highest doses (5% w/w). This finding is of great interest to understand the underlying phenomenon which we assume is the ability of T. castaneum to feed selectively in flour mediums, however, further research on this aspect is required to be investigated. The results of this study support the use of botanicals for stored product pest management.

Occurrence, biochemistry and biological effects of host-selective plant mycotoxins.

Host-selective mycotoxins (HSTs) are various secondary metabolites or proteinaceous compounds secreted by pathogenic necrotrophic fungi that feed off on dead tissues of certain plants. Research on the HSTs has not only fundamental but also practical importance. On one hand they are implicated in the onset of devastating crop diseases. On the other hand, they have been studied as a good model for revealing the intricate mechanisms of plant-pathogen interactions. At the cellular level, HSTs target different compartments and in most instances induce programmed cell death (PCD) by a wide range of mechanisms. Often the responses provoked by HSTs resemble the effector-triggered immunity used by plant cells to combat biotrophic pathogens, which suggests that HST-producing fungi exploit the plants' own defensive systems to derive benefits. Although by definition HSTs are active only in tissues of susceptible plant genotypes, it has been demonstrated that some of them are able to influence animal cells as well. The possible effects, like cytotoxicity or cytostasis, can be harmful or beneficial and thus HSTs may either pose a health risk for humans and livestock, or be of prospective use in the fields of pharmacology, medicine and agriculture.