RESUMO
Neobeguea mahafalensis is used as a medicinal plant in Madagascar. A decoction of the stem bark of this species is reported to treat back pain. Recently, it was reported that a decoction of the root bark, containing two novel phragmalin limonoids as identified active constituents, exhibited an extraordinarily high potency and remarkably long duration in augmenting sexual activity in male rodents.From the dichloromethane extract of the root barks of N. mahafalensis, nine phragmalin limonoids were isolated, of which eight were novel compounds. The structures were established mainly by extensive use of 2D NMR spectroscopic techniques and high-resolution mass spectrometry. One of the new compounds named dodoguin displayed sleep-inducing activity in Swiss albino mice. The amount of 3-10 mg/kg of this compound induced sleep 18-22 min after its administration with a duration of 16-18 min.
Assuntos
Limoninas/isolamento & purificação , Meliaceae/química , Animais , Limoninas/química , Limoninas/farmacologia , Madagáscar , Espectroscopia de Ressonância Magnética , Camundongos , Estrutura Molecular , Plantas Medicinais/química , Medicamentos Indutores do Sono/química , Medicamentos Indutores do Sono/isolamento & purificação , Medicamentos Indutores do Sono/farmacologiaRESUMO
In a screening program directed to the discovery of new anticancer agents from Madagascan plants, ethyl acetate extracts of Croton barorum and C. goudotii showed strong cytotoxic activity, with 100% inhibition at 10 µg/mL in a primary screen using the murine lymphocytic leukemia P388 cell line. Bioassay-guided fractionation led to the isolation of two new 3,4-seco-atisane diterpenoids, crotobarin (1) and crotogoudin (2). Their structures were elucidated by spectroscopic data interpretation. Compounds 1 and 2 produced a net progression in the number of cells arrested at the G2/M growth stage in the cell cycle of the K562 human leukemia cell line at 4 µM.
Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Croton/química , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Animais , Antineoplásicos Fitogênicos/química , Ciclo Celular/efeitos dos fármacos , Croton/genética , Diterpenos/química , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Células K562 , Madagáscar , Camundongos , Estrutura Molecular , Ressonância Magnética Nuclear BiomolecularRESUMO
From stem barks of Capuronianthus mahafalensis (Meliaceae) endemic to Madagascar, a new protolimonoid named capulin containing a four membered ring in its side chain was isolated by repeated silica gel column chromatography. Its structure was determined by 1D and 2D NMR spectroscopy and high-resolution MS. To the best of our knowledge, this is the first time that a four-membered ring occurs in the side chain of protolimonoids.