Detection of receptor tyrosine kinase-orphan receptor-2 using an electrochemical immunosensor modified with electrospun nanofibers comprising polyvinylpyrrolidone, soy, and gold nanoparticles.

An electrochemical immunosensing platform was developed for the detection of receptor tyrosine kinase-orphan receptor-2 (ROR2) at a glassy carbon electrode (GCE) modified with the electrospun nanofiber containing polyvinylpyrrolidone (PVP), soy, and Au nanoparticles (AuNPs). The PVP/soy/AuNP nanofiber exhibited good electrochemical behavior due to synergistic effects between PVP, soy, and AuNPs. The PVP/soy in the modified film provided good mechanical strength, high porosity, flexible structures, and high specific surface area. On the other hand, the presence of AuNPs effectively improved conductivity, as well as the immobilization of anti-ROR2 on the modified GCE, leading to enhanced sensitivity. Various characterization approaches such as FE-SEM, FTIR, and EDS were used for investigating the morphological and structural features, and the elemental composition. The designed immunosensor performance was investigated using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV). Under optimum conditions with a working potential range from -0.2 to 0.6 V (vs. SCE), sensitivity, linear range (LR), limit of detection (LOD), and correlation coefficient (R2) were acquired at 122.26 µA/cm2 dec, 0.01-1000 pg/mL, 3.39 fg/mL, and 0.9974, respectively. Furthermore, the determination of ROR2 in human plasma samples using the designed immunosensing platform was examined and exhibited satisfactory results including good selectivity against other proteins, reproducibility, and cyclic stability.

Aloe vera-loaded nanofibrous scaffold based on Zein/Polycaprolactone/Collagen for wound healing.

Recently, the use of nanofibers (NFs) for tissue engineering has been more developed. For this purpose, we fabricated the NFs (Zein/Polycaprolactone/Collagen) (Zein/PCL/Collagen) incorporated by zinc oxide NPs (ZnO NPs) and Aloe-vera (NFs/ZnO/Alv) using the electrospinning method. Prepared NFs were studied for their morphological, mechanical, thermal stability, and hydrophilic properties. Among the developed NFs, those loaded by ZnO (1 wt%) and Alv (8 wt%) and with Zein/PCL (70:30) displayed the suitable thermal stability and mechanical properties. The water contact angle of NFs improved by decreasing the Zein/PCL blending ratio. Cell culture results showed that the NFs had good cytocompatibility. The cell adhesion potential of this mats were certified with studying by fibroblast cells for various time intervals (24 h and 72 h). The NFs/ZnO/Alv sample revealed inhibition activity against S. aureus (19.23 ± 1.35 mm) and E. coli (15.38 ± 1.12 mm) bacteria. Thus, these results offered that the prepared NFs can be promised as an active scaffold for wound healing uses.

Reinforced ZnONPs/ rosemary essential oil-incorporated zein electrospun nanofibers by κ-carrageenan.

In this study, the reinforced ZnONPs/ rosemary essential oil-incorporated zein nanofibers with κ-carrageenan (Z/KC/ZnONPs/RE) were fabricated using the electrospinning technique for application in food packaging. The SEM images of Z/KC/ZnONPs/RE nanofiber displayed bead-free homogeneous morphology that its average fiber diameter was 672 ±â€¯240 nm. The formation of new hydrogen bonds by addition of κ-carrageenan and active agents was approved by FT-IR and DSC structural conformations. The Z/KC/ZnONPs/RE nanofiber exhibited satisfactory thermal and mechanical properties, as well as high surface hydrophobicity. In addition, the Z/KC/ZnONPs/RE sample showed inhibition activity against S. aureus (18.5 ±â€¯1.9 mm) and E. coli (14.7 ±â€¯1.5 mm) bacteria. The DPPH scavenging activity of Z/KC/ZnONPs/RE nanofiber was 54.5 ±â€¯3.6 %. Additionally, the fabricated nanofibers showed no cell cytotoxicity, indicating their good biocompatibility. Thus, we believe that the fabricated Z/KC/ZnONPs/RE electrospun nanofiber is a potential candidate for using as an active layer in food packaging system.

Study the molecular structure of poly(ε-caprolactone)/graphene oxide and graphene nanocomposite nanofibers.

In this article, poly(ε-caprolactone) (PCL)/graphene oxide (GO) and reduced graphene oxide (RGO) nanocomposite nanofibrous mats were prepared using electrospinning technique. Dynamic mechanical analysis of mats electrospun from solution 16wt% PCL showed 22% and 133% increment in shrink force in the presence of 0.1wt% GO and RGO nanosheets respectively. The creep resistance was also increased 24% and 41%. The good enlargement of molecular chains of PCL at presence of nanosheets and strong chemical interaction within the molecular chains of PCL and nanosheets were caused the increment of shrink force and creep resistance of electrospun mats. However, less relaxation time and higher entanglement density of molecular chains in solution 14wt% and 18wt% PCL respectively limited the interaction within molecular chains of PCL and nanosheets in electrospinning process and thus showed less increment in creep resistance and shrink force of mats electrospun from them. Moreover, tensile stress test of mats electrospun from solution 16wt% PCL showed 53% and 189% increment in the tensile stress at presence of 0.1wt% GO and RGO respectively.

Aligned poly(ε-caprolactone)/graphene oxide and reduced graphene oxide nanocomposite nanofibers: Morphological, mechanical and structural properties.

A number of studies have demonstrated that the mechanical properties of electrospun polymeric nanofibrous scaffolds are enhanced with the incorporation of graphene and its derivatives, thus developing their applications in hard tissue engineering. However, our understanding of the relationship between the microstructure and properties of these fibrous scaffolds and how they are influenced by graphene oxide (GO) and reduced graphene oxide (RGO) loading is much more limited. Thus, in this paper, poly(ε-caprolactone) (PCL)/GO and RGO nanocomposite nanofibers containing 0, 0.1, 0.5 and 1wt.% GO and RGO were prepared using an electrospinning technique. With the addition of 0.1wt.% of GO and RGO nanosheets in PCL, the tensile strength of PCL scaffolds increased over ~160 and 304% respectively and elastic modulus increased over 103 and 163% due to the good dispersion of the nanosheets and their interaction with the molecular chains of PCL. These were supported by the parallel increase in relaxation time and molecular orientation of PCL chains at the presence of nanosheets with a loading of 0.1wt.%. The enhancement effect of the nanosheets was weakened with an increase in GO and RGO loading up to 1wt.% in which it is connected to a partial exfoliation of the nanosheets.