Long noncoding RNAs in development and cancer: potential biomarkers and therapeutic targets.

Long noncoding RNAs are emerging as key players in various fundamental biological processes. We highlight the varied molecular mechanisms by which lncRNAs modulate gene expression in diverse cellular contexts and their role in early mammalian development in this review. Furthermore, it is being increasingly recognized that altered expression of lncRNAs is specifically associated with tumorigenesis, tumor progression and metastasis. We discuss various lncRNAs implicated in different cancer types with a focus on their clinical applications as potential biomarkers and therapeutic targets in the pathology of diverse cancers.

Analysis of in vivo occupancy of Aebp1, a transcription factor, using high resolution tiling array.

Aebp1 is a transcription factor that negatively regulates adipogenesis and also is upregulated in primary GBM as against secondary GBM. Here we describe a systematic approach to map the in vivo occupancy sites of Aebp1 by using a combination of chromatin immune-precipitation technique and hybridization to Agilent high resolution human promoter tiling array (2×224) containing 474393 probes. We also describe the use of various bioinformatics tools for analysis of the hybridization data and identification of Aebp1 occupancy sites in U87MG glioma cell line.