An open-hardware sample mounting solution for inverted light-sheet microscopes with large detection objective lenses.

Implementations of light-sheet microscopes are often incompatible with standard methods of sample mounting. Light-sheet microscopy uses orthogonal illumination and detection to create a thin sheet of light which does not illuminate the sample outside of the depth of field of the detection axis. Typically, this configuration involves a pair of orthogonal objectives which constrains the positioning and length of cover slips in the range of the detection objective. Here, we present an open-hardware sample mounting system for light-sheet microscopes using large detection objectives, built using 3D printed components and demonstrate the chamber's efficacy on live biological samples in a custom light-sheet microscope. LAY DESCRIPTION: Implementations of light-sheet microscopes are often incompatible with standard methods of sample mounting. Light-sheet microscopy creates a thin sheet of light at a certain depth of field within a volumetric sample. Typically, this configuration involves a pair of orthogonal objectives which constrains the positioning of samples and sample-mounting apparatus in range of the detection objective. To overcome the limitations of this setup, we present an open-hardware sample mounting system for light-sheet microscopes using large detection objectives, built using 3D printed components and demonstrate the chamber's efficacy on live biological samples in a custom light-sheet microscope.

Immunocytochemistry in the assessment of pS2 protein expression in fine needle aspiration cytology from breast carcinoma.

pS2 protein is a cysteine-rich polypeptide, of unknown function, the expression of which is induced in the human cancer cell line MCF-7 by oestrogen. The availability of a murine monoclonal antibody to human pS2 protein has prompted us to evaluate its expression in 47 cases of primary breast carcinoma. Using a double indirect immunoperoxidase technique, we compared the expression of pS2 protein in fine needle aspiration (FNA) cytology smears with that in formalin-fixed, paraffin-embedded sections from subsequently excised tumours from the same patients. We also compared the expression of pS2 protein and oestrogen receptor (ER) status using immunocytochemical assay (ER-ICA) in formalin-fixed, paraffin-embedded sections from 22 primary breast carcinomas. We found the application of immunocytochemistry in the assessment of pS2 protein expression in FNA cytology to be a reliable and cost-effective technique, having a sensitivity of 84% and a specificity of 100%. There was also a good correlation between the expression of pS2 protein and ER status.