2.5D microscopy with polarization independent SLM for enhanced detection efficiency and aberration correction.

Fast, volumetric imaging by fluorescence microscopy is essential in studying biological phenomena and cellular functions. Recently, single-shot 2.5D microscopy showed promising results for high-throughput quantitative subcellular analysis via extended depth of field imaging without sequential z-scanning; however, the detection efficiency was limited and it lacked depth-induced aberration correction. Here we report that a spatial light modulator (SLM) in a polarization insensitive configuration can significantly improve the detection efficiency of 2.5D microscopy, while also compensating for aberrations at large imaging depths caused by the refractive index mismatch between the sample and the immersion medium. We highlight the improved efficiency via quantitative single-molecule RNA imaging of mammalian cells with a 2-fold improvement in the fluorescence intensity compared to a conventional SLM-based microscopy. We demonstrate the aberration correction capabilities and extended depth of field by imaging thick specimens with fewer z-scanning steps.

Unidirectional light emission in PT-symmetric microring lasers.

The synergetic use of gain and loss in parity-time symmetric coupled resonators has been shown to lead to single-mode lasing operation. However, at the corresponding resonance frequency, an ideal ring resonator tends to support two degenerate eigenmodes, traveling along the cavity in opposite directions. Here, we show a unidirectional single-moded parity-time symmetric laser by incorporating active S-bend structures with opposite chirality in the respective ring resonators. Such chiral elements break the rotation symmetry of the ring cavities by providing an asymmetric coupling between the clockwise (CW) and the counterclockwise (CCW) traveling modes, hence creating a new type of exceptional point. This property, consequently, leads to the suppression of one of the counter-propagating modes. In this paper, we first measure the extinction ratio between the CW and CCW modes in a single ring resonator in the presence of an S-bend waveguide. We then experimentally investigate the unidirectional emission in PT-symmetric systems below and above the exceptional point. Finally, unidirectional emission will be shown in systems of two S-bend ring resonators coupled through a link structure.

Edge-Mode Lasing in 1D Topological Active Arrays.

We report the first observation of lasing topological edge states in a 1D Su-Schrieffer-Heeger active array of microring resonators. We show that the judicious use of non-Hermiticity can promote single edge-mode lasing in such arrays. Our experimental and theoretical results demonstrate that, in the presence of chiral-time symmetry, this non-Hermitian topological structure can experience phase transitions that are dictated by a complex geometric phase. Our work may pave the way towards understanding the fundamental aspects associated with the interplay among non-Hermiticity, nonlinearity, and topology in active systems.

Comprehensive analysis of imaging quality degradation of an airborne optical system for aerodynamic flow field around the optical window.

We investigated the influences exerted by the nonuniform aerodynamic flow field surrounding the optical window on the imaging quality degradation of an airborne optical system. The density distribution of flow fields around three typical optical windows, including a spherical window, an ellipsoidal window, and a paraboloidal window, were calculated by adopting the Reynolds-averaged Navier-Stokes equations with the Spalart-Allmaras model provided by FLUENT. The fourth-order Runge-Kutta algorithm based ray-tracing program was used to simulate the optical transmission through the aerodynamic flow field. Four kinds of imaging quality evaluation parameters were presented: wave aberration of the entrance pupil, point spread function, encircled energy, and modulation transfer function. The results show that the imaging quality of the airborne optical system was affected by the shape of the optical window and angle of attack of the aircraft.

2.5D microscopy: Fast, high-throughput imaging via volumetric projection for quantitative subcellular analysis.

Imaging-based single-cell analysis is essential to study the expression level and functions of biomolecules at subcellular resolution. However, its low throughput has prevented the measurement of numerous cellular features from multiples cells in a rapid and efficient manner. Here we report 2.5D microscopy that significantly improves the throughput of fluorescence imaging systems while maintaining high-resolution and single-molecule sensitivity. Instead of sequential z-scanning, volumetric information is projected onto a 2D image plane in a single shot by engineering the emitted fluorescence light. Our approach provides an improved imaging speed and uniform focal response within a specific imaging depth, which enabled us to perform quantitative single-molecule RNA measurements over a 2×2 mm2 region within an imaging depth of ~5 µm for mammalian cells in <10 min and immunofluorescence imaging at a >30 Hz volumetric frame rate with reduced photobleaching. Our microscope also offers the ability of multi-color imaging, depth control and super-resolution imaging.

Dust-Sized High-Power-Density Photovoltaic Cells on Si and SOI Substrates for Wafer-Level-Packaged Small Edge Computers.

Advancement in microelectronics technology enables autonomous edge computing platforms in the size of a dust mote (<1 mm), bringing efficient and low-cost artificial intelligence close to the end user and Internet-of-Things (IoT) applications. The key challenge for these compact high-performance edge computers is the integration of a power source that satisfies the high-power-density requirement and does not increase the complexity and cost of the packaging. Here, it is shown that dust-sized III-V photovoltaic (PV) cells grown on Si and silicon-on-insulator (SOI) substrates can be integrated using a wafer-level-packaging process and achieve higher power density than all prior micro-PVs on Si and SOI substrates. The high-throughput heterogeneous integration unlocks the potential of large-scale manufacturing of these integrated systems with low cost for IoT applications. The negative effect of crystallographic defects in the heteroepitaxial materials on PV performance diminishes at high power density. Simultaneous power delivery and data transmission to the dust mote with heteroepitaxially grown PV are also demonstrated using hand-held illumination sources.

Fluorescence imaging with tailored light.

Fluorescence microscopy has long been a valuable tool for biological and medical imaging. Control of optical parameters such as the amplitude, phase, polarization and propagation angle of light gives fluorescence imaging great capabilities ranging from super-resolution imaging to long-term real-time observation of living organisms. In this review, we discuss current fluorescence imaging techniques in terms of the use of tailored or structured light for the sample illumination and fluorescence detection, providing a clear overview of their working principles and capabilities.

Topological insulator laser: Experiments.

Physical systems exhibiting topological invariants are naturally endowed with robustness against perturbations, as manifested in topological insulators-materials exhibiting robust electron transport, immune from scattering by defects and disorder. Recent years have witnessed intense efforts toward exploiting these phenomena in photonics. Here we demonstrate a nonmagnetic topological insulator laser system exhibiting topologically protected transport in the cavity. Its topological properties give rise to single-mode lasing, robustness against defects, and considerably higher slope efficiencies compared to the topologically trivial counterparts. We further exploit the properties of active topological platforms by assembling the system from S-chiral microresonators, enforcing predetermined unidirectional lasing without magnetic fields. This work paves the way toward active topological devices with exciting properties and functionalities.