RESUMO
Bovine mastitis is an inflammation of the mammary gland in response to invasion by opportunistic agents. Due to the high economic importance of dairy production and the complexity related to animal health, the objective of this work was to identify and evaluate the antibacterial resistance profile of samples of mastitis milk, milking hand and milking equipment from small rural dairy farms belonging to the northwest region of the state of Paraná, Brazil. Five small, non-technical dairy farms in the municipalities of Boa Esperança, Juranda and Tapejara, all belonging to the northwest region of the state of Paraná, Brazil, were selected. The properties had Holstein and/or crossbred herds, carried out a bucket-by-foot milking system and all had the presence of animals with subclinical mastitis confirmed by the California Mastitis Test. Samples of sterile swabs from the milking insufflators, the milking hand and milk samples were collected-and later, isolation tests and phenotypic characterization of the samples, sensitivity tests to antimicrobials and phenotypic tests for the detection of beta-producing strains were performed with extended-spectrum beta-lactamase (ESBL), molecular identification of Staphylococcus aureus isolates and mecA gene research. Of the 199 samples collected from the 15 selected properties in the municipalities of Boa Esperança, Tapejara and Juranda, 72 (36.20%) were classified as multiresistant. Isolated from milkers' hands and milking machines, which phenotypically produce extended-spectrum beta-lactamase (ESBL), the presence of the mecA gene was also observed in 11 isolates of Staphylococcus spp. of milk samples, machines and milking hands. Mastitis can be spread to the herd through the milking process by the milkers' instruments and hands, and adequate management measures can prevent its transmission and the conscious use of antibiotics decreases the prevalence of multidrug-resistant pathogens. In this work, different pathogenic bacteria were detected in mastitic milk, milking equipment and milking hand with a high percentage (36.20%) of isolates classified as multidrug resistant. In addition, the presence phenotypically (ESBL) and molecularly (mecA gene) of isolates carrying resistance genes was also verified. These results directly reflect on the health of the animals, the health of the workers and the health of the respective environment, which can enable the continuity of the propagation of the etiological agents involved in the mastitis infection. The awareness of producers and workers on these properties about the disease, transmission, sanitary aspects and adequate management and treatment are essential for improving milk production and production efficiency.
RESUMO
Purpose: To evaluate the prevalence of signs and symptoms of temporomandibular disorders (TMD) in children and the impact on their oral health-related quality of life (OHRQoL).
Methods: Data were collected by means of a questionnaire to evaluate the presence of TMD symptoms in eight to 10-year-old children. For those who answered at least one of the four questions in a positive manner, a clinical exam was done to confirm the diagnosis. The Child Perceptions Questionnaire (CPQ8-10) was applied to evaluate the OHRQoL. Poisson regression was used to associate the total scores and individual domains of the CPQ8-10 with sociodemographic factors and clinical conditions.
Results: A total of 245 children participated in this cross-sectional study. One hundred and twenty-one (49.4 percent) were diagnosed with TMD; 57.9 percent were females and 42.1 percent were males. Children with a diagnosis of disc displacement with reduction had a negative impact on the domain of functional limitations (rate ratio=2.61, 95 percent confidence interval=1.19 to 5.75, P =0.017).
Conclusion: The prevalence of signs and symptoms of TMD was high in our sample, and signs and symptoms of TMD had a negative impact on children's OHRQoL in the domain of functional limitations.