The metabolic profiles of Eugenia astringens and E. uniflora (Myrtaceae) sensitive seeds affect desiccation.

Myrtaceae species are abundant in tropical Atlantic rainforests, but 41% of the 5500 species of this family are of extreme conservation concern. Eugenia astringens and E. uniflora are native Brazilian Myrtaceae species that occur in the same habitats and produce desiccation-sensitive (DS) seeds. We hypothesized that their seed desiccation-sensitivity degree is associated with specific metabolic signatures. To test it, we analyzed the germination and metabolic profiles of fresh and desiccated seeds. The water content (WC) at which at least half of the seeds survived desiccation was lower in E. astringens (0.17 g H2 O g-1 DW) than in E. uniflora (0.41 g H2 O g-1 DW). We identified 103 annotated metabolites from 3261 peaks in both species, which differed in their relative contents between E. astringens and E. uniflora seeds. The main differences in seed metabolic profiles include several protective molecules in the group of carbohydrates and organic acids and amino acid contents. The relative contents of monosaccharides and disaccharides, malic and quinic acids, amino acids and saturated fatty acids may have taken part in the distinct DS behaviour of E. astringens and E. uniflora seeds. Our study provides evidence of the relationship between desiccation sensitivity, seed viability and metabolic profile of tropical seeds by comparing two closely related Eugenia species with different DS degrees.

Effect of preemptive use of a nonsteroidal anti-inflammatory drug and a corticosteroid on the efficacy of inferior alveolar nerve blockade and postoperative pain control in endodontic treatment of molars with symptomatic pulpitis: A randomized double-blind placebo-controlled clinical trial.

AIM: The anaesthetic success rate of an inferior alveolar nerve block (IANB) in mandibular molars with irreversible symptomatic pulpitis can be low, and postoperative pain control in teeth with this diagnosis can be challenging. This study aimed to evaluate the influence of preemptive use of dexamethasone and oral potassium diclofenac on the success of IANB. The influence of these drugs on the intensity of postoperative pain was assessed as a secondary outcome. METHODOLOGY: Eighty-four patients with mandibular molars diagnosed with irreversible symptomatic pulpitis recorded preoperative pain intensity using a cold thermal test and a modified Numerical Rating Scale (mNRS). Sixty minutes before the anaesthetic procedure, patients were randomly assigned to one of three groups based on the medication they received: dexamethasone (4 mg), diclofenac potassium (50 mg), or placebo. All patients received IANB with 4% articaine (1:200 000 epinephrine), and 15 min later, they were evaluated for pain intensity using the cold thermal test. Anaesthetic success was analysed. The pain intensity was then recorded, and endodontic treatment and provisional restoration of the tooth were executed in a single session. Patients were monitored for 6, 12, 24, 48 and 72 h using the mNRS to assess the intensity of postoperative pain. RESULTS: There was a statistically significant increase in anaesthetic success when 4 mg dexamethasone (39.3%) or 50 mg diclofenac potassium (21.4%) was used compared to the placebo group (3.6%) (p < .001), with no significant difference between the two drugs. Regarding postoperative pain, dexamethasone was superior to placebo at 6 h (p < .001), with diclofenac having an intermediate behaviour, not differing between dexamethasone and placebo (p > .05). There was no significant difference amongst the groups at 12 h (p > .05). At 24, 48 and 72 h, the effectiveness of dexamethasone and diclofenac were comparable, and both were superior to placebo (p < .001). CONCLUSION: The use of dexamethasone or diclofenac potassium was favourable in terms of increasing the success rate of inferior alveolar nerve block in cases of mandibular molars with irreversible symptomatic pulpitis and decreased the occurrence of postoperative pain when compared to the use of a placebo.

Effects of Isocaloric Resistance, Aerobic, and Concurrent Exercise on Excess Postexercise Oxygen Consumption in Older Adults.

ABSTRACT: Pilon, R, Matos-Santos, L, Matlez, MP, Rodrigues, G, Amorim, F, Lattari, E, Farinatti, P, and Monteiro, W. Effects of isocaloric resistance, aerobic, and concurrent exercise on excess postexercise oxygen consumption in older adults. J Strength Cond Res 38(4): 755-761, 2024-Excess postexercise oxygen consumption (EPOC) is a major determinant of exercise-related caloric expenditure and metabolic adaptations. Exercise modality may influence the EPOC, but this issue has not been investigated in older adults. This study compared the EPOC after isocaloric bouts of continuous aerobic exercise (AE), resistance exercise (RE), and concurrent exercise (CE) in older individuals. Ten subjects (5 men; 73 ± 6 years) had their cardiorespiratory data assessed during AE, RE, and CE and along 30-minute postexercise recovery. Total energy expenditure (EE) during exercise was similar (p > 0.05) in AE (126.0 ± 30.7 kcal), RE (123.9 ± 30.6 kcal), and CE (130.8 ± 32.6 kcal), with different times to achieve the targeted EE (RE: 61.4 ± 1.9 minutes > CE: 43.3 ± 5.6 minutes > AE: 26.6 ± 5.7 minutes; p < 0.001). Consistently, the relative intensity during exercise was superior (p < 0.05) in AE (74 ± 15% oxygen uptake reserve [VO2R]) vs. CE (43 ± 13% VO2R) vs. RE (24 ± 9% VO2R). Despite the isocaloric conditions, average EPOC and EE were approximately 45% greater (p < 0.001) in AE (8.0 ± 2.3 L; 40.1 ± 11.7 kcal) vs. RE (5.6 ± 1.2 L; 28.1 ± 5.8 kcal) and CE (5.4 ± 2.3 L; 26.9 ± 11.5 kcal). In conclusion, the EPOC was greater after isocaloric AE vs. RE and CE performed by older adults. Exercise intensity seemed to be a more important determinant of EPOC than volume reflected by EE during exercise bouts. Moderate-intensity continuous AE was more time-efficient than RE and CE to achieve a target EE. In older individuals, AE should be preferred over RE or CE when the purpose is to increase the daily caloric expenditure.

White Matter Hyperintensities and Poststroke Apathy: A Fully Automated MRI Segmentation Study.

INTRODUCTION: Poststroke apathy (PSA) is a common neuropsychiatric disorder that may affect up to 30% of stroke patients. Despite the difficulties of investigating this condition (overlapping with depression, heterogeneity of diagnostic criteria, a small number of studies), some recent diffusion tensor imaging studies have suggested that widespread microstructural white matter (WM) disruption plays a key role in the development of PSA. Therefore, we intended to investigate this hypothesis by evaluating the relationship between WM hyperintensities (WMH) and apathy in patients with cerebrovascular disease. METHODS: We studied patients with apathy (n = 7), depression (n = 13), comorbid apathy and depression (n = 13), and controls (n = 20), and we investigated the variables associated with the volume of WMH measured by an automated brain MRI segmentation software. RESULTS: The overall prevalence of PSA was 37.7% (pure and comorbid). Patients with apathy presented a higher volume of WMH in comparison to controls. Mini-Mental State Examination (MMSE), NPI-A, and the number of cerebral microbleeds were the only variables associated with WMH. Conversely, NPI-D did not correlate to WMH. DISCUSSION/CONCLUSION: This is an exploratory study that supports the view of PSA as a distinct syndrome from PSD mediated mainly by diffuse white matter hyperintensities, which suggests that WM disruption is an important pathway to the development of apathy in stroke patients.

Self-selected or fixed: is there an optimal rest interval for controlling intensity in high-intensity interval resistance training?

PURPOSE: This study investigated the effects of different rest interval strategies during high-intensity interval resistance training (HIRT) on cardiorespiratory, perceptual, and enjoyment responses among trained young men. METHODS: Sixteen men experienced with HIRT underwent cardiopulmonary exercise testing and were familiarized with the exercises and HIRT protocol. On the subsequent three visits, interspaced 48-72 h, participants performed HIRT sessions with different rest intervals in a randomized order: 10 s and 30 s fixed rest intervals (FRI-10 and FRI-30), and self-selected rest interval (SSRI). Oxygen uptake (VO2), heart rate (HR), and recovery perception (Total Quality Recovery Scale) were measured during HIRT, while enjoyment responses (Physical Activity Enjoyment Scale) were assessed immediately after the sessions. RESULTS: The VO2 during exercise was greater in FRI-10 than FRI-30 (55% VO2max and 47% VO2max, respectively, p = 0.01), while no difference occurred between SSRI and bouts performed with fixed intervals (52% VO2max vs. FRI, p > 0.05). HR, excess post-exercise oxygen consumption (EPOC), recovery perception, and enjoyment responses were similar across conditions (p > 0.05). CONCLUSION: Exercise intensity was not affected by the rest interval strategy. High exercise intensity was maintained in sessions performed with FRI or SSRI, without negative repercussions on the duration of training sessions and enjoyment responses after exercise sessions.

Intracellular reactive oxygen species trafficking participates in seed dormancy alleviation in Arabidopsis seeds.

Reactive oxygen species (ROS) release seed dormancy through an unknown mechanism. We used different seed dormancy-breaking treatments to decipher the dynamics and localization of ROS production during seed germination. We studied the involvement of ROS in the breaking of Arabidopsis seed dormancy by cold stratification, gibberellic acid (GA3 ) and light. We characterized the effects of these treatments on abscisic acid and gibberellins biosynthesis and signalling pathways. ROS, mitochondrial redox status and peroxisomes were visualized and/or quantified during seed imbibition. Finally, we performed a cytogenetic characterization of the nuclei from the embryonic axes during seed germination. We show that mitochondria participate in the early ROS production during seed imbibition and that a possible involvement of peroxisomes in later stages should still be analysed. At the time of radicle protrusion, ROS accumulated within the nucleus, which correlated with nuclear expansion and chromatin decompaction. Taken together, our results provide evidence of the role of ROS trafficking between organelles and of the nuclear redox status in the regulation of seed germination by dormancy.

What affects the desiccation tolerance threshold of Brazilian Eugenia (Myrtaceae) seeds?

Desiccation sensitive (DS) seeds are shed at high water contents (WC) and metabolically active, but WC thresholds vary broadly among species even in the same genus. Eugenia is an important ecological genus that has high occurrence in several Brazilian morphoclimatic domains. In this study, we assessed seed desiccation tolerance of five Eugenia species collected in specific meteorological conditions. We reported the species geographical ranges and verified the rainfall and temperature of species sites in the year prior to seed collection. We also assessed initial WC, seed germination and vigor and seedling growth upon desiccation. Eugenia uniflora was the widest spread among the five species, while E. astringens was the most restricted. In this specific study, widespread species showed a higher WC threshold than restricted species. In the same way, the WC of fresh seeds was not correlated to the desiccation tolerance threshold. Seed desiccation tolerance was species dependent and correlated with the environmental status of seed collection sites. Wetter and warmer conditions were correlated to the E. uniflora higher DS threshold. Low rainfall and temperature corresponded to a lower desiccation sensitivity of E. astringens seeds. Seeds of the five species lost half viability between 0.44 and 0.25 g H2O g DW- 1 and after 65-270 h of desiccation. Our results indicate that abiotic factors impact plant populations during the seed production season and can drive seed desiccation tolerance threshold and physiological behavior. These results should be taken into account in ex-situ plant conservation programs and tropical species management.

Effects of Anodal Transcranial Direct Current Stimulation on Training Volume and Pleasure Responses in the Back Squat Exercise Following a Bench Press.

ABSTRACT: Rodrigues, GM, Machado, S, Faria Vieira, LA, Ramalho de Oliveira, BR, Jesus Abreu, MA, Marquez, G, Maranhão Neto, GA, and Lattari, E. Effects of anodal transcranial direct current stimulation on training volume and pleasure responses in the back squat exercise following a bench press. J Strength Cond Res 36(11): 3048-3055, 2022-This study aimed to investigate the effects of anodal transcranial direct current stimulation (a-tDCS) on volume-load and pleasure responses in a back squat following a bench press. Twelve male subjects advanced in resistance training (RT) (age, 25.5 ± 4.4 years) completed 2 experimental trials in a counterbalanced crossover design: a-tDCS and sham conditions. The stimulus was applied over the left dorsolateral prefrontal cortex for 20 minutes using a 2-mA current intensity in a-tDCS condition and 1 minute of active stimulus in the sham condition. Immediately after stimulation, subjects performed the bench press followed by the back squat. The exercise protocol consisted of 3 sets of maximum repetitions at an intensity of 80% of 1 repetition maximum. The volume-load, perceived pleasure, and arousal responses were measured during the RT protocol. The results indicated that volume-load was higher in the a-tDCS condition than in the sham condition for both exercises ( p = 0.02), with large effect for the back squat ( p = 0.045; d = 0.96). The higher volume-load was obtained by increasing the number of repetitions across all sets for the bench press ( p ≤ 0.0001) and only in the first set for the back squat ( p = 0.01). The circumplex model analysis showed a higher pleasure in the bench press and a tendency toward a higher pleasure in the a-tDCS condition. Anodal tDCS may be used as an ergogenic resource for increasing the back squat volume after performing the bench press in resistance-trained male subjects.

Anodal Transcranial Direct Current Stimulation Increases Muscular Strength and Reduces Pain Perception in Women With Patellofemoral Pain.

ABSTRACT: Rodrigues, GM, Paixão, A, Arruda, T, de Oliveira, BRR, Maranhão Neto, GA, Marques Neto, SR, Lattari, E, and Machado, S. Anodal transcranial direct current stimulation increases muscular strength and reduces pain perception in women with patellofemoral pain. J Strength Cond Res 36(2): 371-378, 2022-The purpose of this study is to investigate the effects of anodic transcranial direct current stimulation applied to motor cortex combined with open kinetic chain exercises on muscular strength and pain perception in women with patellofemoral pain (PFP). Twenty-eight women aged between 18 and 30 years with PFP were selected. Subjects were randomized in 2 groups, anodic stimulus plus resistance training (n = 14; anodic transcranial direct current stimulation [a-tDCS] + RT) or placebo stimulus plus resistance training (n = 14; Sham + RT) and attended the laboratory for 12 experimental sessions, 48-72 hours apart from each other. The RT protocol consisted of 3 sets of 12 repetitions of the knee extension exercise at 60% of 10 maximal repetition (10RM) with a 1-minute interval between sets. In the a-tDCS + RT group, a 2-mA current was applied for 20 minutes over the motor cortex before the RT protocol in each session. In the Sham + RT group, the stimulus was interrupted after 30 seconds. Preintervention, fourth session, eighth session, and postintervention, load was assessed through a 10RM test. The pain perception was assessed through Clarke sign maneuver (CSM) and measured through a visual analogue scale for pain. The a-tDCS + RT group showed greater 10RM load than Sham + RT group at eighth session (p < 0.05) and postintervention (p < 0.05). In a-tDCS + RT group, pain perception reduced in the postintervention compared with preintervention (p < 0.05). The intervention a-tDCS + RT was able to improve muscular strength in women with PFP. In addition, pain perception only decreased postintervention in the a-tDCS + RT group. This combined intervention can be used by coaches in rehabilitation programs aiming to treat PFP through medium-term strength gains.

Recovery of Nonlinear Heart Rate Variability After Submaximal Exercise in Young Persons With Symptoms of Anxiety and Depression.

Prior studies have demonstrated that anxiety and depression explain the increase of adverse cardiovascular events an failure to modulate cardiac activity. This study of the nonlinear heart rate (HR) variability (HRV) behavior can provide additional information concerning the autonomic recovery of HR after exercise. The dynamics of these indices in exercise-mediated situations may reveal other ways to assess HRV recovery after physical effort. We studied nonlinear HRV recovery after submaximal exercise in subjects with higher Hospital Anxiety and Depression Scale (HADS) scores. Sixty-six young adults were recruited, and 50 completed the HADS rating scale to quantify their degree of anxiety and depression for later allocation to a suitable group. After experimental procedures, the final sample involved 20 participants (15 female) who were allocated to the group with low HADS scores (LHADS) and 21 (16 female) to the group with high HADS scores (HHADS). We logged HRV data before and during recovery from submaximal aerobic exercise and analyzed this data using symbolic analysis. Young adults with High HADS scores (HHADS) had a slower recovery of the symbolic analysis of HRV via index 2LV% (two like variations) and 2ULV% (two unlike variations) after aerobic exercise. Participants with higher HADS scores presented delayed nonlinear HRV recovery after submaximal exercise.

Quality of Movement Is Associated With Cognitive Function in Physically Inactive Older Women.

The relationship between the quality of movement, considering different global and universal basic patterns of movement and cognition domains in older adults remain unclear. The current study explored this association in physically inactive older women. In total, 187 participants, aged 60-70 years (mean = 64.9, SD = 6.9 years), were recruited from a physical education program in a public university. The older adults performed the following tests: Functional Movement Screen, Montreal Cognitive Assessment, and Modified Baecke Questionnaire for the Older Adults. The regression analysis showed an association between age (ß = -0.11, 95% confidence interval, CI, [-0.10, 0.30], p = .03); visuospatial abilities (ß = 0.36, 95% CI [0.24, 1.23], p < .001); language (ß = 0.23, 95% CI [0.20, 1.08], p < .001); and orientation domains (ß = 0.13, 95% CI [0.11, 1.22], p = .016) of the Montreal Cognitive Assessment and the Functional Movement Screen. The quality of movement was related to both age and cognitive performance, such as the visuospatial abilities, language, and orientation domains, in physically inactive older women.

Biomarkers Related to Endothelial Dysfunction and Vascular Cognitive Impairment: A Systematic Review.

INTRODUCTION: The damage in the endothelium and the neurovascular unit appears to play a key role in the pathogenesis of vascular cognitive impairment (VCI). Although there have been many advances in understanding the physiopathology of this disease, several questions remain unanswered. The association with other degenerative diseases and the heterogeneity of its clinical spectrum establish a diagnostic problem, compromising a better comprehension of the pathology and halting the development of effective treatments. The investigation of biomarkers is an important movement to the development of novel explicative models and treatment targets involved in VCI. METHODS: We searched MEDLINE considering the original research based on VCI biomarkers in the past 20 years, following prespecified selection criteria, data extraction, and qualitative synthesis. RESULTS: We reviewed 42 articles: 16 investigated plasma markers, 17 analyzed neuropathological markers, 4 studied CSF markers, 4 evaluated neuroimaging markers (ultrasound and MRI), and 1 used peripheral Doppler perfusion imaging. CONCLUSIONS: The biomarkers in these studies suggest an intrinsic relationship between endothelial dysfunction and VCI. Nonetheless, there is still a need for identification of a distinctive set of markers that can integrate the clinical approach of VCI, improve diagnostic accuracy, and support the discovery of alternative therapies.

Extended Time Window Mechanical Thrombectomy for Acute Stroke in Brazil.

BACKGROUND: Mechanical thrombectomy (MT) is the standard of care for acute ischemic stroke (AIS) caused by large vessel occlusion of the anterior circulation within 6 hours of symptoms onset and can be performed with an extended window up to 24 hours in selected patients. Nevertheless, the outcomes of MT with extended window are unknown in developing countries. OBJECTIVE: Explore the safety and efficacy of MT for AIS performed beyond 6 hours from symptoms onset in Brazil. METHODS: We reviewed data from AIS patients treated with MT beyond 6 hours of stroke onset, from 2015 to 2018 in a Brazilian public hospital. Patients had an occlusion of the intracranial internal carotid artery and/or proximal segment of the middle cerebral artery. CT Perfusion mismatch was evaluated using the RAPID® software. We evaluated the modified Rankin scale (mRS) and mortality at 90 days, and rate of symptomatic intracranial hemorrhage (sICH). RESULTS: Fifty-four patients were included, with a mean age of 65.6 ± 16.1 years, 55.6% were male, and the median NIHSS score at presentation was 17. Successful recanalization (TICI 2b to 3) was obtained in 92.6% of patients and sICH rate was 11.1%. Overall, 34% of the patients had a good outcome (mRS ≤2) at 90 days and the mortality rate was 20.3%. CONCLUSION: Our study, the first series of MT for AIS treated with extended window reported in Latin America, shows that MT can be performed with safety and lead to adequate functional outcomes in this context. Further studies should explore the barriers to broad implementation of MT for AIS in Latin America.

Selective separation of Cu, Ni and Ag from printed circuit board waste using an environmentally safe technique.

Printed circuit boards (PCBs) make up a large part of e-waste and include high concentrations of high-value metals. Therefore, the recovery of these metals is interesting from both the environmental and economic points of view. Here, the extraction/separation of copper, nickel and silver from PCB leachate was studied using an aqueous two-phase system (ATPS) formed by triblock copolymers with an electrolyte and water, which is in compliance with the principles of green chemistry. The best conditions for the selective extraction consisted of 1-(2-pyridylazo)-2-naphthol (3.5 mmol kg-1) at pH = 6.0 in 6 sequential steps for the Cu(II), dimethylglyoxime (5.00 mmol kg-1) at pH = 9.0 for the Ni(II) and thiocyanate (5.20 mmol kg-1) at pH = 9.0 for the Ag(I). These conditions were applied sequentially for extraction of Cu, Ni and Ag from the PCB leachate, obtaining high separation factor (S) values between the analyte and the metallic concomitants (SCu,Ni = 1,460, SCu,Fe = 15,500, SCu,Ag = 15,900, SNi,Fe = 32,700, SNi,Ag = 34,700 and SAg,Fe = 4800). The maximum extraction percentages (%E) for Cu, Ni and Ag were 99.9%, 99.9% and 99.8%, respectively. After the extraction, a single step stripping process was performed, resulting in more than 82% of the ion available in a clean lower phase. For the first time, an ATPS has been used for sequential extraction of several metal analytes from a real sample.

Use of aqueous two-phase PEG-salt systems for the removal of anionic surfactant from effluents.

Linear alkylbenzene sulfonates (LAS) are synthetic anionic surfactants that are extensively used in many industries. As a result, large volumes of effluents containing high levels of these compounds are discharged into water bodies, causing risks to aquatic flora and fauna. Then, there is a need for environmentally safe and economically viable technologies for the removal of LAS from aqueous matrices. The present work evaluates the use of aqueous two-phase systems (ATPS) composed of PEG and sulfate salts for this purpose, considering the effects of tie line length (TLL), molar mass of polymer, and type of cation-forming salt on the partitioning behavior of LAS. All the LAS partition coefficient (KLAS) values were greater than unity, and the LAS extraction efficiencies (%ELAS) were higher than 97%. The system consisting of PEG 1500 + (NH4)2SO4 + H2O provided the highest KLAS (1083.34) and %ELAS (99.9%), indicating that the method provided good extraction of LAS to the top phase. This system was applied using a real effluent sample in laboratory-scale experiments as well as in bench-scale batch trials. The results obtained at the laboratory scale showed %ELAS values greater than 98%, while the best KLAS value obtained in the batch experiments was 8.50 (±1.75) (%ELAS = 78.17%). These values demonstrated the potential of ATPS for the removal of LAS from industrial effluents.

A method for dye extraction using an aqueous two-phase system: Effect of co-occurrence of contaminants in textile industry wastewater.

This paper reports a green and efficient procedure for extraction of the dyes Malachite Green (MG), Methylene Blue (MB), and Reactive Red 195 (RR) using an aqueous two-phase system (ATPS). An ATPS consists mainly of water, together with polymer and salt, and does not employ any organic solvent. The extraction efficiency was evaluated by means of the partition coefficients (K) and residual percentages (%R) of the dyes, under different experimental conditions, varying the tie-line length (TLL) of the system, the pH, the type of ATPS-forming electrolyte, and the type of ATPS-forming polymer. For MG, the best removal (K = 4.10 × 10(4), %R = 0.0069%) was obtained with the ATPS: PEO 1500 + Na2C4H4O6 (TLL = 50.21% (w/w), pH = 6.00). For MB, the maximum extraction (K = 559.9, %R = 0.258%) was achieved with the ATPS: PEO 400 + Na2SO4 (TLL = 50.31% (w/w), pH = 1.00). Finally for RR, the method that presented the best results (K = 3.75 × 10(4), %R = 0.237%) was the ATPS: PEO 400 + Na2SO4 (TLL = 50.31% (w/w), pH = 6.00). The method was applied to the recovery of these dyes from a textile effluent sample, resulting in values of K of 1.17 × 10(4), 724.1, and 3.98 × 10(4) for MG, MB, and RR, respectively, while the corresponding %R values were 0.0038, 0.154, and 0.023%, respectively. In addition, the ATPS methodology provided a high degree of color removal (96.5-97.95%) from the textile effluent.

Neurodevelopmental disorders associated with dosage imbalance of ZBTB20 correlate with the morbidity spectrum of ZBTB20 candidate target genes.

BACKGROUND: Recently, a number of patients have been described with structural rearrangements at 3q13.31, delineating a novel microdeletion syndrome with common clinical features including developmental delay and other neurodevelopmental disorders (NDD). A smallest region of overlapping deletions (SRO) involved five RefSeq genes, including the transcription factor gene ZBTB20 and the dopamine receptor gene DRD3, considered as candidate genes for the syndrome. METHODS AND RESULTS: We used array comparative genomic hybridization and next-generation mate-pair sequencing to identify key structural rearrangements involving ZBTB20 in two patients with NDD. In a patient with developmental delay, attention-deficit hyperactivity disorder, psychosis, Tourette's syndrome and autistic traits, a de novo balanced t(3;18) translocation truncated ZBTB20. The other breakpoint did not disrupt any gene. In a second patient with developmental delay and autism, we detected the first microdeletion at 3q13.31, which truncated ZBTB20 but did not involve DRD3 or the other genes within the previously defined SRO. Zbtb20 directly represses 346 genes in the developing murine brain. Of the 342 human orthologous ZBTB20 candidate target genes, we found 68 associated with NDD. Using chromatin immunoprecipitation and quantitative PCR, we validated the in vivo binding of Zbtb20 in evolutionary conserved regions in six of these genes (Cntn4, Gad1, Nrxn1, Nrxn3, Scn2a, Snap25). CONCLUSIONS: Our study links dosage imbalance of ZBTB20 to a range of neurodevelopmental, cognitive and psychiatric disorders, likely mediated by dysregulation of multiple ZBTB20 target genes, and provides new knowledge on the genetic background of the NDD seen in the 3q13.31 microdeletion syndrome.

Validation of "CENTR(AR)" walking trails: Different field criteria do not lead to different physical activity intensities in people with COPD.

BACKGROUND: Validating walking trails is essential to promote physical activity (PA) safely and confidently in people with COPD. OBJECTIVES: We aimed to validate predetermined light, moderate, and vigorous intensities of walking trails in people with COPD. METHODS: This cross-sectional study included individuals with COPD walking in predetermined light, moderate and vigorous intensity trails. Activity intensity and volume outcomes were collected. Dyspnoea and fatigue (modified Borg scale), energy expenditure (EE, Sensewear), heart rate (HR, HR monitor), time spent in different PA intensities, and cadence (ActiGraph) were recorded and used to classify PA intensity. RESULTS: Twenty people with COPD [71(7) years, 80 % male, FEV1%predicted 65.6(11.6)] were included. Fatigue differed significantly between light and moderate [3.0(2.0;4.0) vs 3.4(2.5;4.5), p = 0.01], but not vigorous (3.5[2.5-4.0]) tracks. Dyspnoea [2.3(1.5) vs 2.7(1.6) vs 2.6(1.4)], EE [5.1(0.8) vs 4.9(0.5) vs 4.6(0.8) METs], HR [92.5(11.1) vs 93.7(18.6) vs 95.4(15.0) beats/min] and cadence [115.1(104.0;120.3) vs 104.7(99.6;117.6) vs 111.2(99.9;118.5) steps/min] were similar across trails (p > 0.05). Time spent in light and moderate PA, EE volume, walking time, and step count increased along with the proposed intensity levels (p < 0.01). Walking trails were categorised as moderate intensity in most participants. CONCLUSION: Walking trails were safe and valid for practising moderate-intensity PA in people with COPD. Participants adjusted their physiological responses and perceived symptoms to match a moderate intensity.

Sleep quality of patients with diabetes mellitus: association with anxiety trait and state.

OBJECTIVE: To assess sleep quality and the association of trait and state anxiety in patients with diabetes mellitus. METHOD: A cross-sectional, quantitative, and analytical study was conducted between January and April 2022, at the reference center for hypertension and diabetes in Rio Verde, Goiás, with 81 patients. The Pittsburgh Sleep Quality Index and State-Trait Anxiety Inventory were used for data collection. Analyses included the Student's t-test and multivariate logistic regression analysis. RESULTS: Of patients, 64.2% reported poor sleep quality. There were significant differences in trait anxiety levels between patients with good and poor sleep quality. Trait anxiety was associated with sleep quality, with an increase in the trait anxiety score increasing the chances of poor sleep quality. CONCLUSION: A significant proportion of the sample had poor sleep quality, and trait anxiety was found to be associated with this condition.

Genome-Wide Admixture and Association Study of Serum Selenium Deficiency to Identify Genetic Variants Indirectly Linked to Selenium Regulation in Brazilian Adults.

Blood selenium (Se) concentrations differ substantially by population and could be influenced by genetic variants, increasing Se deficiency-related diseases. We conducted a genome-wide association study (GWAS) to identify single nucleotide polymorphisms (SNPs) associated with serum Se deficiency in 382 adults with admixed ancestry. Genotyping arrays were combined to yield 90,937 SNPs. R packages were applied to quality control and imputation. We also performed the ancestral proportion analysis. The Search Tool for the Retrieval of Interacting Genes was used to interrogate known protein-protein interaction networks (PPIs). Our ancestral proportion analysis estimated 71% of the genome was from Caucasians, 22% was from Africans, and 8% was from East Asians. We identified the SNP rs1561573 in the TraB domain containing 2B (TRABD2B), rs425664 in MAF bZIP transcription factor (MAF), rs10444656 in spermatogenesis-associated 13 (SPATA13), and rs6592284 in heat shock protein nuclear import factor (HIKESHI) genes. The PPI analysis showed functional associations of Se deficiency, thyroid hormone metabolism, NRF2-ARE and the Wnt pathway, and heat stress. Our findings show evidence of a genetic association between Se deficiency and metabolic pathways indirectly linked to Se regulation, reinforcing the complex relationship between Se intake and the endogenous factors affecting the Se requirements for optimal health.