RESUMO
A cellular DNA binding protein, LBP-1, sequentially interacts in a concentration-dependent manner with two sites that surround the transcriptional initiation site of the human immunodeficiency virus type 1 (HIV-1) promoter. Although sequences in the downstream site (site I) were found to enhance transcription, purified LBP-1 specifically repressed transcription in vitro by binding to the upstream site (site II), which overlaps the TATA element. The binding of human TATA binding factor (TFIID) to the promoter before LBP-1 blocked repression, suggesting that repression resulted from an inhibition of TFIID binding to the TATA element. Furthermore, mutations that eliminated binding to site II both prevented repression in vitro and increased HIV-1 transcription in stably transformed cells. These findings suggest that a cellular factor regulates HIV-1 transcription in a manner that is characteristic of bacterial repressors and that this factor could be important in HIV-1 latency.
Assuntos
Regulação Viral da Expressão Gênica , HIV-1/genética , Proteínas Repressoras/genética , Sequência de Bases , Proteínas de Ligação a DNA/genética , Dados de Sequência Molecular , Regiões Promotoras Genéticas , Fator de Transcrição TFIID , Fatores de Transcrição/metabolismo , Transcrição GênicaRESUMO
TFIID is a multisubunit protein complex comprised of the TATA-binding protein (TBP) and multiple TBP-associated factors (TAFs). The TAFs in TFIID are essential for activator-dependent transcription. The cloning of a complementary DNA encoding a human TFIID TAF, TAFII55, that has no known homolog in Drosophila TFIID is now described. TAFII55 is shown to interact with the largest subunit (TAFII230) of human TFIID through its central region and with multiple activators--including Sp1, YY1, USF, CTF, adenoviral E1A, and human immunodeficiency virus-type 1 Tat proteins--through a distinct amino-terminal domain. The TAFII55-interacting region of Sp1 was localized to its DNA-binding domain, which is distinct from the glutamine-rich activation domains previously shown to interact with Drosophila TAFII110. Thus, this human TFIID TAF may be a co-activator that mediates a response to multiple activators through a distinct mechanism.
Assuntos
Fatores Associados à Proteína de Ligação a TATA , Transativadores/metabolismo , Fatores de Transcrição/química , Fatores de Transcrição/metabolismo , Proteínas E1A de Adenovirus/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Proteínas de Ligação a DNA/metabolismo , Fatores de Ligação de DNA Eritroide Específicos , Produtos do Gene tat/metabolismo , Células HeLa , Humanos , Dados de Sequência Molecular , Fator de Transcrição Sp1/química , Fator de Transcrição Sp1/metabolismo , Proteína de Ligação a TATA-Box , Transativadores/química , Transativadores/genética , Fator de Transcrição TFIID , Fatores Estimuladores Upstream , Fator de Transcrição YY1RESUMO
Transcription of protein-encoding genes by human RNA polymerase II requires multiple ancillary proteins (transcription factors). Interactions between these proteins and the promoter DNA of a viral class II gene (the major late transcription unit of adenovirus) were investigated by enzymatic and chemical footprinting. The experiments indicated that the assembly of functionally active RNA polymerase II-containing transcription preinitiation complexes requires a complete set of transcription factors, and that both specific protein-DNA and protein-protein interactions are involved. This allows individual steps along the transcription reaction pathway to be tested directly, thus providing a basis for understanding basic transcription initiation mechanisms as well as the regulatory processes that act on them.
Assuntos
Regiões Promotoras Genéticas , RNA Polimerase II/metabolismo , Sequências Reguladoras de Ácido Nucleico , Fatores de Transcrição/fisiologia , Transcrição Gênica , Adenovírus Humanos/genética , Sequência de Bases , Proteínas de Ligação a DNA/fisiologia , Desoxirribonucleases/metabolismo , Substâncias Macromoleculares , Dados de Sequência Molecular , Proteínas Nucleares/fisiologiaRESUMO
Class III genes require multiple cellular factors for transcription by RNA polymerase III; these genes form stable transcription complexes, which in the case of Xenopus 5S genes are correlated with differential expression in vivo. The minimal number and identity of the factors required to form both stable and metastable complexes on three class III genes (encoding, respectively, 5S RNA, transfer RNA, and adenovirus VA RNA species) were determined. Stable complex formation requires one common factor, whose recognition site was analyzed, and either no additional factors (the VA gene), a second common factor (the transfer RNA gene), or a third gene-specific factor (the 5S gene). The mechanism of stable complex formation and its relevance to transcriptional regulation were examined in light of the various factors and the promoter sequences recognized by these factors.
Assuntos
RNA Polimerases Dirigidas por DNA/genética , RNA Polimerase III/genética , Transcrição Gênica , Animais , Sequência de Bases , Células Eucarióticas/fisiologia , Regulação da Expressão Gênica , Genes , Humanos , Óperon , RNA Ribossômico/genética , RNA de Transferência/genética , RNA Viral/genética , Fatores de Transcrição/genéticaRESUMO
The discovery of TATA-binding protein-related factors (TRFs) has suggested alternative mechanisms for gene-specific transcriptional regulation and raised interest in their biological functions. In contrast to recent observations of an embryonic lethal phenotype for TRF2 inactivation in Caenorhabditis elegans and Xenopus laevis, we found that Trf2-deficient mice are viable. However, Trf2-/- mice are sterile because of a severe defect in spermiogenesis. Postmeiotic round spermatids advance at most to step 7 of differentiation but fail to progress to the elongated form, and gene-specific transcription deficiencies were identified. We speculate that mammals may have evolved more specialized TRF2 functions in the testis that involve transcriptional regulation of genes essential for spermiogenesis.
Assuntos
Proteínas de Ligação a DNA/deficiência , Proteínas de Ligação a DNA/fisiologia , Deleção de Genes , Espermatogênese/genética , Animais , Apoptose , Diferenciação Celular , Tamanho Celular , Proteínas de Ligação a DNA/genética , Feminino , Regulação da Expressão Gênica no Desenvolvimento , Marcação de Genes , Genótipo , Infertilidade Masculina/genética , Infertilidade Masculina/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Tamanho do Órgão , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Espermatozoides/metabolismo , Espermatozoides/patologia , Proteína 2 de Ligação a Repetições Teloméricas , Testículo/anormalidades , Testículo/metabolismo , Testículo/patologiaRESUMO
alpha-Amanitin, a toxic substance from the mushroom Amanita phalloides, is a potent inhibitor of DNA-dependent RNA polymerase II (the nucleoplasmic form) from sea urchin, rat liver, and calf thymus. This compound exerts no effect on the activity of polymerase I (nucleolar form) or polymerase III (also nucleoplasmic). The inhibition is due to a specific interaction with polymerase II or with a complex of DNA and polymerase II.
Assuntos
Núcleo Celular/enzimologia , Micotoxinas/farmacologia , RNA Nucleotidiltransferases/antagonistas & inibidores , Animais , Bovinos , DNA , Equinodermos , Escherichia coli/enzimologia , Fígado/citologia , Fígado/enzimologia , Ratos , Timo/citologia , Timo/enzimologiaRESUMO
Transcription initiation factor TFIIB recruits RNA polymerase II to the promoter subsequent to interaction with a preformed TFIID-promoter complex. The domains of TFIIB required for binding to the TFIID-promoter complex and for transcription initiation have been determined. The carboxyl-terminal two-thirds of TFIIB, which contains two direct repeats and two basic residue repeats, is sufficient for interaction with the TFIID-promoter complex. An extra 84-residue amino-terminal region, with no obvious known structural motifs, is required for basal transcription activity. Basic residues within the second basic repeat of TFIIB are necessary for stable interaction with the TFIID-promoter complex, whereas the basic character of the first basic repeat is not. Functional roles of other potential structural motifs are discussed in light of the present study.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Regiões Promotoras Genéticas , Fatores de Transcrição/química , Fatores de Transcrição/metabolismo , Sequência de Aminoácidos , Animais , Sítios de Ligação , Drosophila , Dados de Sequência Molecular , Mutação , Ligação Proteica , Fator de Transcrição TFIIB , Fator de Transcrição TFIIDRESUMO
Octamer transcription factor-1 (OTF-1) and nuclear factor III (NF-III) are sequence-specific DNA binding proteins that activate transcription and DNA replication, respectively. It is shown here that OTF-1 is physically and biologically indistinguishable from NF-III. This conclusion is based on the following observations. First, the two proteins have identical mobilities by SDS-polyacrylamide gel electrophoresis. Second, OTF-1 binds to the adenovirus origin of DNA replication at the same site and with the same affinity as NF-III. Third, OTF-1 can substitute for NF-III in activating the initiation of adenovirus DNA replication in vitro. Fourth, the ability of OTF-1 to stimulate viral DNA replication is dependent on the presence of an intact NF-III binding site within the origin of replication. Fifth, NF-III can substitute for OTF-1 in activating in vitro transcription from the human histone H2b promoter. These data suggest the possibility that NF-III/OTF-1 is a protein that functions in both cellular DNA replication and transcription.
Assuntos
Replicação do DNA/efeitos dos fármacos , Proteínas de Ligação a DNA/farmacologia , Proteínas Nucleares/farmacologia , Fatores de Transcrição/farmacologia , Transcrição Gênica/efeitos dos fármacos , Adenoviridae/fisiologia , Proteínas de Ligação a DNA/metabolismo , Eletroforese em Gel de Poliacrilamida , Células HeLa , Histonas/genética , Fator C1 de Célula Hospedeira , Humanos , Peso Molecular , Proteínas Nucleares/metabolismo , Fator 1 de Transcrição de Octâmero , Regiões Promotoras Genéticas , Fatores de Transcrição/metabolismo , Replicação Viral/efeitos dos fármacosRESUMO
Individual steps in the assembly of RNA polymerase II and general initiation factors into a preinitiation complex serve as points of control for activators, whose functions require additional cofactors. The full range of induction by activators appears to involve both the reversal of negative constraints and net positive effects on promoter interactions with general factors.
Assuntos
Células Eucarióticas , Regulação da Expressão Gênica , Fatores de Transcrição , Sequência de Aminoácidos , Animais , Sequência de Bases , Dados de Sequência MolecularRESUMO
Transcription initiation on protein-encoding genes represents a major control point for gene expression in eukaryotes, and is mediated by RNA polymerase II and a surprisingly complex array of general initiation factors (TFIIA, -B, -D, -E, -F and -H) that are highly conserved from yeast to man. Elucidation of structural and functional features of these factors on model promoters has revealed insights into biochemical mechanisms and provides a basis for understanding their regulation on diverse promoters by gene- and cell-specific activators.
Assuntos
RNA Polimerase II/genética , RNA Polimerase II/metabolismo , Fatores de Transcrição/genética , Transcrição Gênica , Animais , Proteínas de Ligação a DNA/genética , Previsões , Humanos , Modelos Genéticos , Regiões Promotoras Genéticas , RNA Polimerase II/química , TATA Box , Proteína de Ligação a TATA-Box , Fator de Transcrição TFIIA , Fator de Transcrição TFIIB , Fatores de Transcrição/metabolismoRESUMO
A novel multiprotein complex has recently been identified as a coactivator for transcriptional control of protein-encoding genes by RNA polymerase II in higher eukaryotic cells. This complex is evolutionarily related to the Mediator complex from yeast and, on the basis of its structural and functional characteristics, promises to be a key target of diverse regulatory circuits.
Assuntos
Regulação Enzimológica da Expressão Gênica , Invertebrados/genética , RNA Polimerase II/genética , Transcrição Gênica , Ativação Transcricional , Leveduras/genética , Animais , Humanos , Modelos Biológicos , Peptídeos/químicaRESUMO
Selective and accurate transcription of purified genes by RNA polymerase II requires multiple factors. The factor designated TFIID was purified extensively from HeLa cell nuclear extracts by using a simple and novel complementation assay. Thus, TFIID was preferentially inactivated by mild heat treatment of a nuclear extract, and supplementation of the heat-treated extract with TFIID-containing fractions restored adenovirus major late (ML) promoter-dependent transcription. By using this assay, TFIID was purified approximately 300-fold by conventional chromatographic methods. The most purified TFIID fraction was demonstrated to be required for transcription of a number of other cellular and viral class II genes. This factor showed specific interactions with both the adenovirus ML promoter and a human heat shock 70 (hsp-70) promoter. On the ML promoter, the DNase I-protected region extended from around position -40 to position +35, although some discontinuities (and associated hypersensitive sites) were apparent near the initiation site and near position +27; the upstream and downstream boundaries of the TFIID-binding site were also confirmed by exonuclease III digestion experiments. In contrast to these results, the DNase I-protected regions on the human hsp-70 promoter were confined to a smaller area that extended from positions -35 to -19. DNase I hypersensitive sites were observed in both the adenovirus ML and hsp-70 promoters, most notably in the region at position -47. These results indicate either that there are different forms of TFIID or that a single TFIID can interact differently with distinct promoters.
Assuntos
Regiões Promotoras Genéticas , RNA Polimerase II/metabolismo , Fatores de Transcrição/isolamento & purificação , Fatores de Transcrição/fisiologia , Transcrição Gênica , Adenoviridae/genética , Sequência de Bases , Células HeLa , Proteínas de Choque Térmico/genética , Dados de Sequência Molecular , Sequências Reguladoras de Ácido Nucleico , Fator de Transcrição TFIIDRESUMO
A 215-base-pair (bp) region of the mouse MOPC 41 kappa light-chain immunoglobulin gene enhancer has been analyzed for specific binding of lymphoid and nonlymphoid nuclear factors. Mobility shift assays with a series of overlapping DNA fragments have mapped DNA-binding sites for three unique factors. The B-cell-specific (OTF-2) and ubiquitous (OTF-1) octamer-binding transcription factors specifically bound to a site centered about 136 bp 5' of the nuclear factor NF-kappa B site. A third specific factor, NF-kappa E, bound to a site that was about 75 bp 5' of the NF-kappa B site and within a region important for enhancer function. This novel factor was found in both mature B and HeLa cell nuclei. B-cell OTF-2, B-cell OTF-1, and HeLa OTF-1 bound to the kappa enhancer and kappa promoter octamer sites with similar affinities despite a 2-bp difference in the kappa enhancer octamer sequence. However, DNase I footprint analyses indicated that affinity-purified OTF-2 bound both to the enhancer OTF site and, surprisingly, to 80 bp of A + T-rich flanking sequence. Moreover, methylation interference studies demonstrated distinct differences in OTF interactions between the consensus octamer in the kappa promoter and the nonconsensus octamer identified in the enhancer. This novel observation of an OTF-binding site in the kappa enhancer provides a common link with the OTF sites in the promoter-proximal regions of all kappa promoters and thus mirrors the structural arrangement of OTF sites found in the promoters and enhancers of immunoglobulin heavy-chain genes.
Assuntos
Linfócitos B/fisiologia , Elementos Facilitadores Genéticos/genética , Cadeias kappa de Imunoglobulina/genética , Animais , Sequência de Bases , Ligação Competitiva , Linhagem Celular , Análise Mutacional de DNA , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/fisiologia , Desoxirribonuclease I , Humanos , Metilação , Camundongos , NF-kappa B , Proteínas Nucleares/metabolismo , Sondas de Oligonucleotídeos/metabolismo , Sequências Reguladoras de Ácido Nucleico , Fatores de Transcrição/genética , Fatores de Transcrição/fisiologiaRESUMO
Late in infection, transcription of the EIIa gene is initiated primarily at map unit 72 of the adenovirus genome. A cell-free nuclear extract system was used to determine sequence elements important for the function of this late promoter. In such a system, the transcriptional activity of a circular template was found to be much higher (5- to 10-fold) than that of a linear template. The effect of template topology appeared to be dependent on two distal upstream elements with 5' boundaries located near -265 to -223 and -147 to -133 (in relation to the initiation site), since deletions of these regions reduced transcription of the circular template, in a stepwise fashion, to a level similar to that observed with the linear template. Further deletions revealed an element in the -116 region that appeared to be more important for transcription of the circular template (10-fold reduction) than for transcription of the linear template (3-fold reduction). Lastly, deletion of the TACAAA sequence in the -29 region resulted in further reduction in transcription, indicating that this element functions as a promoter in vitro.
Assuntos
Adenoviridae/genética , DNA Viral/genética , Proteínas de Ligação a DNA/genética , Genes Virais , Proteínas Oncogênicas Virais/genética , Regiões Promotoras Genéticas , Sequências Reguladoras de Ácido Nucleico , Transcrição Gênica , Proteínas Precoces de Adenovirus , DNA Circular/metabolismo , DNA Viral/metabolismo , Conformação de Ácido NucleicoRESUMO
Immunoglobulin heavy-chain genes contain two conserved sequence elements 5' to the site of transcription initiation: the octamer ATGCAAAT and the heptamer CTCATGA. Both of these elements are required for normal cell-specific promoter function. The present study demonstrates that both the ubiquitous and lymphoid-cell-specific octamer transcription factors (OTF-1 and OTF-2, respectively) interact specifically with each of the two conserved sequence elements, forming either homo- or heterodimeric complexes. This was surprising, since the heptamer and octamer sequence motifs bear no obvious similarity to each other. Binding of either factor to the octamer element occurred independently. However, OTF interaction with the heptamer sequence appeared to require the presence of an intact octamer motif and occurred with a spacing of either 2 or 14 base pairs between the two elements, suggesting coordinate binding resulting from protein-protein interactions. The degeneracy in sequences recognized by the OTFs may be important in widening the range over which gene expression can be modulated and in establishing cell type specificity.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Genes de Imunoglobulinas , Regiões Promotoras Genéticas , Fatores de Transcrição/metabolismo , Sequência de Bases , Sítios de Ligação , DNA/genética , DNA/metabolismo , Células HeLa/metabolismo , Fator C1 de Célula Hospedeira , Humanos , Linfócitos/metabolismo , Dados de Sequência Molecular , Fator 1 de Transcrição de Octâmero , Fator 2 de Transcrição de Octâmero , Transcrição GênicaRESUMO
Human transcription factor TFIID contains the TATA-binding protein (TBP) and several TBP-associated factors (TAF(II)s). To elucidate the structural organization and function of TFIID, we expressed and characterized the product of a cloned cDNA encoding human TAF(II)135 (hTAF(II)135). Comparative far Western blots have shown that hTAF(II)135 interacts strongly with hTAF(II)20, moderately with hTAF(II)150, and weakly with hTAF(II)43 and hTAF(II)250. Consistent with these observations and with sequence relationships of hTAF(II)20 and hTAF(II)135 to histones H2B and H2A, respectively, TFIID preparations that contain higher levels of hTAF(II)135 also contain higher levels of hTAF(II)20, and the interaction between hTAF(II)20 and hTAF(II)135 is critical for human TFIID assembly in vitro. From a functional standpoint, hTAF(II)135 has been found to interact strongly and directly with hTFIIA and (within a complex that also contains hTBP and hTAF(II)250) to specifically cooperate with TFIIA to relieve TAF(II)250-mediated repression of TBP binding and function on core promoters. Finally, we report a functional synergism between TAF(II)s and the TRAP/Mediator complex in activated transcription, manifested as hTAF(II)-mediated inhibition of basal transcription and a consequent TRAP requirement for both a high absolute level of activated transcription and a high and more physiological activated/basal transcription ratio. These results suggest a dynamic TFIID structure in which the switch from a basal hTAF(II)-enhanced repression state to an activator-mediated activated state on a promoter may be mediated in part through activator or coactivator interactions with hTAF(II)135.
Assuntos
Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/fisiologia , Proteínas Nucleares/química , Proteínas Nucleares/fisiologia , Fatores Associados à Proteína de Ligação a TATA , Fatores de Transcrição TFII/química , Transcrição Gênica , Western Blotting , Cromatografia em Gel , Clonagem Molecular , DNA Complementar/metabolismo , Desoxirribonuclease I/metabolismo , Histona Acetiltransferases , Histonas/química , Humanos , Modelos Genéticos , Regiões Promotoras Genéticas , Ligação Proteica , Estrutura Terciária de Proteína , Transativadores/metabolismo , Transativadores/fisiologia , Fator de Transcrição TFIIA , Fator de Transcrição TFIID , Fatores de Transcrição/químicaRESUMO
We examined the transcription of a variety of adenovirus type 2 genes in a cell-free system containing purified ribonucleic acid polymerase II and a crude extract from cultured human cells. The early EIA, EIB, EIII, and EIV genes and the intermediate polypeptide IX gene, all of which contain a recognizable TATAA sequence upstream from the cap site, were actively transcribed in vitro, albeit with apparently different efficiencies, whereas the early EII (map position 74.9) and IVa2 genes, both of which lack a TATAA sequence, were not actively transcribed. A reverse transcriptase-primer extension analysis showed that the 5' ends of the in vitro transcripts were identical to those of the corresponding in vivo ribonucleic acids and that, in those instances where initiation was heterogeneous in vivo, a similar kind of heterogeneity was observed in the cell-free system. Transcription of the polypeptide IX gene indicated that this transcript was not terminated at, or processed to, the polyadenylic acid addition site in vitro. We also failed to observe, using the in vitro system, any indication of transcriptional regulation based on the use of adenovirus type 2-infected cell extracts.
Assuntos
Adenovírus Humanos/genética , Regiões Promotoras Genéticas , Transcrição Gênica , Sistema Livre de Células , Células Cultivadas , Mapeamento Cromossômico , Genes Virais , Humanos , RNA Polimerase II/metabolismoRESUMO
The tissue-specific expression of the MOPC 141 immunoglobulin heavy-chain gene was studied by using in vitro transcription. B-cell-specific transcription of this gene was dependent on the octamer element 5'-ATGCAAAG-3', located in the upstream region of this promoter and in the promoters of all other immunoglobulin heavy- and light-chain genes. The interaction of purified octamer transcription factors 1 and 2 (OTF1 and OTF2) with the MOPC 141 promoter was studied by using electrophoretic mobility shift assays and DNase I footprinting. Purified OTF1 from HeLa cells and OTF1 and OTF2 from B cells bound to identical sequences within the heavy-chain promoter. The OTF interactions we observed extended over the heptamer element 5'-CTCAGGA-3', and it seems likely that the binding of the purified factors involves cooperation between octamer and heptamer sites in this promoter. In addition to these elements, we identified a second regulatory element, the N element with the sequence 5'-GGAACCTCCCCC-3'. The N element could independently mediate low levels of transcription in both B-cell and HeLa-cell extracts, and, in conjunction with the octamer element, it can promote high levels of transcription in B-cell extracts. The N element bound a transcription factor, NTF, that is ubiquitous in cell-type distribution, and NTF was distinct from any of the previously described proteins that bind to similar sequences. Based on these results, we propose that NTF and OTF2 interactions (both with their cognate DNA elements and possibly at the protein-protein level) may be critical to B-cell-specific expression and that these interactions provide additional pathways for regulating gene expression.
Assuntos
Genes de Imunoglobulinas , Cadeias Pesadas de Imunoglobulinas/genética , Linfócitos/fisiologia , Regiões Promotoras Genéticas , Sequências Reguladoras de Ácido Nucleico , Fatores de Transcrição/fisiologia , Sequência de Bases , Linhagem Celular , Proteínas de Ligação a DNA/genética , Regulação da Expressão Gênica , Humanos , Técnicas In Vitro , Dados de Sequência Molecular , Transcrição GênicaRESUMO
USF is a cellular factor involved in the transcriptional regulation of several cellular and viral promoters. Purified USF from HeLa cells (HeLa USF) consists of 43- and 44-kDa polypeptides which show independent binding to a specific DNA element. A cDNA encoding the 43-kDa species has been previously cloned. We show here that the purified form of bacterially expressed 43-kDa USF (i) exists in solution as a dimer whose formation is greatly favored under reducing conditions, (ii) binds to its cognate DNA sequence in a manner indistinguishable from that of HeLa USF, and (iii) is as efficient as HeLa USF in stimulating transcription from target promoters in a reconstituted cell-free system. Additional data indicate that the 44-kDa component of HeLa USF is immunologically unrelated to the 43-kDa polypeptide but is associated with it in HeLa cell extracts. These results suggest that the 43-kDa component possesses an intrinsic DNA binding and transcriptional activation potential and that the 44-kDa USF component of the natural USF complex may have some regulatory role.
Assuntos
Proteínas de Ligação a DNA , DNA/metabolismo , Fatores de Transcrição/metabolismo , Sequência de Bases , Western Blotting , Sistema Livre de Células , Células HeLa , Humanos , Substâncias Macromoleculares , Dados de Sequência Molecular , Regiões Promotoras Genéticas/fisiologia , Proteínas Recombinantes/metabolismo , Fagos T/genética , Transcrição Gênica/fisiologia , Fatores Estimuladores UpstreamRESUMO
We have purified the c-fos enhancer-binding protein from HeLa cell nuclear extracts. The key purification steps involved chromatography on a nonspecific DNA affinity column, from which binding activity and other protein were eluted at low salt concentrations, followed by chromatography on a specific oligonucleotide affinity column, from which the enhancer binding activity was specifically eluted at high salt concentrations. The purified protein had a strong affinity for the c-fos enhancer dyad symmetry sequence, with an equilibrium dissociation constant of 3.3 x 10(-11) M. This affinity was at least 50,000-fold stronger than that found for nonspecific DNA sequences.