RESUMO
The H-Y locus is on the short arm of the human Y chromosome in most individuals but on the long arm in at least one of 17 individuals with structural abnormalities of the Y.
Assuntos
Antígenos de Histocompatibilidade/genética , Aberrações dos Cromossomos Sexuais/imunologia , Cromossomos Sexuais , Cromossomo Y , Centrômero , Inversão Cromossômica , Mapeamento Cromossômico , Feminino , Humanos , MasculinoRESUMO
A 12-yr-old hypothyroid girl was diagnosed at birth as athyreotic because her thyroid gland could not be visualized by isotope scanning. Goiter development due to incomplete thyrotropin suppression, a thyroidal radioiodide uptake of < 1%, and a low saliva to plasma ratio of 2.5 suggested iodide (I-) transport defect. mRNA isolated from her thyroid gland and injected into Xenopus oocytes failed to increase I- transport. Sequencing of the entire Na+/I- symporter (NIS) cDNA revealed a C to G transversion of nucleotide (nt) 1146 in exon 6, resulting in a Gln 267 (CAG) to Glu (GAG) substitution. This missense mutation produces an NIS with undetectable I- transport activity when expressed in COS-7 cells. Although only this missense mutation was identified in thyroid and lymphocyte cDNA, genotyping revealed that the proposita and her unaffected brother and father were heterozygous for this mutation. However, amplification of cDNA with a primer specific for the wild-type nt 1146 yielded a sequence lacking 67 nt. Genomic DNA showed a C to G transversion of nt 1940, producing a stop codon as well as a new downstream cryptic 3' splice acceptor site in exon 13, responsible for the 67 nt deletion, frameshift, and premature stop predicting an NIS lacking 129 carboxy-terminal amino acids. This mutation was inherited from the mother and present in the unaffected sister. Thus, although the proposita is a compound heterozygote, because of the very low expression (< 2.5%) of one mutant allele, she is functionally hemizygous for an NIS without detectable bioactivity.
Assuntos
Proteínas de Transporte/genética , Hipotireoidismo/genética , Proteínas de Membrana/genética , Simportadores , Alelos , Substituição de Aminoácidos , Animais , Células COS , Proteínas de Transporte/metabolismo , Células Cultivadas , Criança , Códon de Terminação , Hipotireoidismo Congênito , DNA/análise , DNA/genética , DNA Antissenso/genética , DNA Complementar/genética , Éxons , Feminino , Mutação da Fase de Leitura , Expressão Gênica , Heterozigoto , Humanos , Hipotireoidismo/etiologia , Linfócitos/metabolismo , Masculino , Proteínas de Membrana/metabolismo , Linhagem , Plasmídeos , Mutação Puntual , RNA/análise , RNA/genética , Splicing de RNA , Deleção de Sequência , Glândula Tireoide/metabolismo , XenopusRESUMO
Modification of the natural vasopressin molecule to form desmopressin acetate (DDAVP) resulted in a compound with prolonged antidiuretic activity and virtual elimination of vasopressor activity. Twenty-one patients with central diabetes insipidus who ranged in age from 3 to 68 years were treated with DDAVP, which was administered intranasally in a dosage ranging from 10 microgram every 12 hours to 20 microgram every eight hours. Effective control of symptoms was obtained in all cases. There were no consequential toxic effects. As previously reported, DDAVP appears to be the preferred drug for the management of central diabetes insipidus. Biochemical alteration of hormones may enhance desired therapeutic activity and eliminate toxic effects. The development of DDAVP is an example of the potential for development of useful therapeutic peptides.
Assuntos
Desamino Arginina Vasopressina/uso terapêutico , Diabetes Insípido/tratamento farmacológico , Vasopressinas/análogos & derivados , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Desamino Arginina Vasopressina/administração & dosagem , Desamino Arginina Vasopressina/efeitos adversos , Feminino , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
A 17-year-old boy with partial hypoxanthine-guanine phosphoribosyl transferase deficiency developed a hypersensitivity reaction to allopurinol. The reaction was manifested by the development of bizarre, atypical seizures. The patient had been neurologically normal prior to the reaction. Seizures disappeared following discontinuation of allopurinol therapy. Allopurinol apparently can cause a diffuse vasculitis involving cerebral vessels after many year of therapy, resulting in atypical seizures.
Assuntos
Alopurinol/efeitos adversos , Arterite/induzido quimicamente , Doenças Arteriais Cerebrais/induzido quimicamente , Hipoxantina Fosforribosiltransferase/deficiência , Adolescente , Arterite/etiologia , Doenças Arteriais Cerebrais/etiologia , Hipersensibilidade a Drogas/complicações , Humanos , Masculino , Convulsões/induzido quimicamenteRESUMO
Two cases of persistent hematuria associated with the presence of sickle cell hemoglobin were treated intravenously with triglycyl vasopressin, a drug not previously used for this condition. One patient, a 16-year-old boy, had hemoglobin AS. Both patients had a history of severe hematuria persisting over several months, resistant to the usual forms of therapy, and requiring numerous transfusions. In each patient, the condition responded to intravenous triglycyl vasopression therapy, with cessation of hematuria. Experimental studies in dogs indicate that triglycyl vasopressin reduces renal blood flow substantially. Further trial of triglycyl vasopressin in severe hematuria associated with the presence of sickle cell hemoglobin appears to be indicated.
Assuntos
Anemia Falciforme/urina , Hematúria/tratamento farmacológico , Hemoglobina Falciforme/análise , Lipressina/análogos & derivados , Adolescente , Adulto , Anemia Falciforme/diagnóstico , Anemia Falciforme/fisiopatologia , Animais , Pressão Sanguínea/efeitos dos fármacos , Cães , Contagem de Eritrócitos , Feminino , Frequência Cardíaca/efeitos dos fármacos , Hematúria/diagnóstico , Hematúria/fisiopatologia , Humanos , Lipressina/uso terapêutico , Masculino , Pulso Arterial/efeitos dos fármacos , Fluxo Sanguíneo Regional/efeitos dos fármacos , Artéria Renal/efeitos dos fármacos , TerlipressinaRESUMO
Women with congenital adrenal hyperplasia due to 21-hydroxylase deficiency often have a polycystic ovary-like syndrome, consisting of hyperandrogynism, infertility, menstrual irregularities, and elevated LH levels. This is generally considered secondary to poor control of the congenital adrenal hyperplasia. However, our experience led us to suspect that ovarian hyperandrogenism occurs even when congenital adrenal hyperplasia is well controlled on glucocorticoid therapy. Therefore, we tested the hypothesis that congenital adrenal virilizing disorders result in ovarian hyperandrogenism. We studied eight women with congenital adrenal virilizing disorders, seven with well controlled classic 21-hydroxylase deficiency and one with congenital virilizing adrenal carcinoma removed at 1.7 yr of age. We also studied six women with late-onset 21-hydroxylase deficiency, without signs of congenital virilization. An ovarian source of androgens was assessed after suppressing adrenal function with dexamethasone and then testing pituitary-ovarian function by a GnRH agonist (nafarelin) test. Five women with congenital adrenal virilizing disorders (four with classic 21-hydroxylase deficiency and one with congenital virilizing adrenal carcinoma) and one women with late-onset 21-hydroxylase deficiency had ovarian hyperandrogenism as determined by subnormal suppression of free testosterone after dexamethasone and/or by increased 17-hydroxyprogesterone response to nafarelin while on dexamethasone. All women with congenital adrenal virilization and ovarian hyperandrogenism had elevated LH levels after dexamethasone or elevated early LH response to nafarelin, which suggests that LH excess is the cause of their ovarian hyperandrogenism. This was not the case for the late-onset 21-hydroxylase-deficient woman. Our data are compatible with the hypothesis that congenital adrenal virilization programs the hypothalamic-pituitary axis for hypersecretion of LH at puberty. This is postulated to frequently cause ovarian hyperandrogenism even when adrenal androgen excess is subsequently controlled by glucocorticoid therapy.
Assuntos
Doenças das Glândulas Suprarrenais/congênito , Doenças das Glândulas Suprarrenais/complicações , Hiperandrogenismo/etiologia , Sistemas Neurossecretores/fisiologia , Doenças Ovarianas/etiologia , Virilismo/fisiopatologia , Adolescente , Doenças das Glândulas Suprarrenais/metabolismo , Neoplasias das Glândulas Suprarrenais/complicações , Neoplasias das Glândulas Suprarrenais/congênito , Neoplasias das Glândulas Suprarrenais/metabolismo , Adulto , Criança , Dexametasona/farmacologia , Feminino , Humanos , Hidrolases/deficiência , Hiperandrogenismo/metabolismo , Hiperandrogenismo/fisiopatologia , Hormônio Luteinizante/metabolismo , Nafarelina/farmacologia , Sistemas Neurossecretores/efeitos dos fármacos , Sistemas Neurossecretores/metabolismo , Doenças Ovarianas/fisiopatologia , Caracteres Sexuais , Testosterona/metabolismo , Virilismo/metabolismoRESUMO
The pituitary-gonadal axis was evaluated in a mother after two of her sons with familial male-limited pseudoprecocious puberty were found to have a constitutively activating mutation of the LH receptor (LHR). Genotyping demonstrated that all showed a mutation in one of the two alleles, a substitution of Gly for Asp578 in the sixth transmembrane segment of the LHR. Ovarian function was normal in the 36-yr-old mother as assessed by LH dynamics and FSH and androgen levels throughout the menstrual cycle. Hormonal responses to acute GnRH agonist (nafarelin) challenge, chronic GnRH agonist administration, and dexamethasone were also normal. Studies of the boys upon presentation at 2.4 and 3.5 yr of age revealed that acute LH responses to nafarelin were in the hypogonadotropic range, and the FSH responses were prepubertal despite the presence of late pubertal testosterone blood levels. Upon the inception of true puberty at 11 yr of age in the older brother, gonadotropin responses normalized for the state of development. The data show that this activating LHR mutation does not cause functional ovarian hyperandrogenism and causes only incomplete pubertal activation of Leydig cells. The results are compatible with relatively low constitutive activity associated with this structural abnormality of LHR.
Assuntos
Hormônios , Mutação , Nafarelina , Puberdade Precoce/genética , Receptores do LH/genética , Adulto , Androgênios/sangue , Pré-Escolar , Dexametasona , Estradiol/sangue , Feminino , Hormônio Foliculoestimulante/sangue , Genótipo , Hormônios/administração & dosagem , Humanos , Hormônio Luteinizante/sangue , Masculino , Nafarelina/administração & dosagem , Linhagem , Testosterona/sangueRESUMO
We have previously demonstrated that the tissue-specific regulation of human aromatase cytochrome P450 (P450arom) gene expression is, in part, the consequence of the use of tissue-specific promoters. Promoter I.1 (PI.1) and PI.2-specific transcripts are expressed in the placenta, whereas promoter II (PII) appears to be the only active promoter in the corpus luteum. Testicular and ovarian sex cord tumors with annular tubules (SCTATs) associated with gynecomastia in prepubertal boys and isosexual precocity in girls with Peutz-Jeghers syndrome (P-JS) have been previously reported. In the present study, we investigated the regulatory elements directing P450arom gene transcription in samples of SCTAT from three prepubertal boys and a girl with P-JS and an ovarian granulosa cell tumor from an adult woman, as well as in healthy fetal and adult testicular and ovarian tissues. Placental tissue was used as a control. Using polymerase chain reaction linked to reverse transcription and northern blotting, we determined the tissue-specific use of various P450arom promoters by analyzing specific 5'-termini from messenger RNA templates. Results indicate a universal gonadal promoter (PII) directs P450arom gene expression in healthy fetal and adult ovaries and testes, as well as in SCTAT of the P-JS and an adult ovarian granulosa cell tumor. These results are interpreted to mean that use of PII in human ovary and testis is preserved from the fetal period into adult life as well as in transformed neoplastic Sertoli and granulosa cells. On the other hand, transcripts from placenta are specific for PI.1 (and to a much lesser extent, PI.2). In SCTAT, immunoreactive P450arom is detected only in the cytoplasm of neoplastic cells, whereas the normal-appearing sex cords do not contain any immunoreactive P450arom. These results further suggest that the markedly increased aromatase expression of these transformed neoplastic cells is not a consequence of using different tissue-specific promoters. Rather it appears to involve activation (or failure of inhibition) of the upstream regulatory elements of the same promoter, which is normally functional in all gonadal tissues, namely the proximal PII.
Assuntos
Aromatase/genética , Regulação Enzimológica da Expressão Gênica , Gônadas/enzimologia , Neoplasias Ovarianas/enzimologia , Tumores do Estroma Gonadal e dos Cordões Sexuais/enzimologia , Neoplasias Testiculares/enzimologia , Adolescente , Adulto , Sequência de Bases , Criança , Pré-Escolar , Feminino , Feto/metabolismo , Humanos , Masculino , Dados de Sequência Molecular , Gravidez , Regiões Promotoras GenéticasRESUMO
The testes of five phenotypic women (from four families) with 5 alpha-reductase deficiency were studied. In one of the patients, the enzyme deficiency was similar in the testis and epididymis and in fibroblasts cultured from the labia majora. In testes from four of the patients, the concentrations of the 5 alpha-reduced steroids dihydrotestosterone and 3 alpha-androstanediol were less than 10% of those in normal subjects. We conclude that the testis is involved in 5 alpha-reductase deficiency. Impaired spermatogenesis was evident in testicular biopsies from all five subjects, and in two, sperm production, as estimated in testicular homogenates, was less than 10% of normal. The extent to which spermatogenic arrest is due to 5 alpha-reductase deficiency or testicular maldescent is not clear.
Assuntos
3-Oxo-5-alfa-Esteroide 4-Desidrogenase/deficiência , Transtornos do Desenvolvimento Sexual/enzimologia , Testículo/enzimologia , Adolescente , Adulto , Androgênios/metabolismo , Transtornos do Desenvolvimento Sexual/patologia , Epididimo/enzimologia , Feminino , Fibroblastos/enzimologia , Humanos , Masculino , Fenótipo , Receptores Androgênicos/metabolismo , Túbulos Seminíferos/patologia , Pele/enzimologia , Espermatogênese , Testículo/patologiaRESUMO
We have previously demonstrated that the tissue-specific regulation of human aromatase cytochrome P450 (P450arom) gene expression is, in part, the consequence of the use of tissue-specific promoters. Promoter I.1 (PI.1) and PI.2-specific transcripts are expressed in the placenta, whereas promoter II (PII) appears to be the only active promoter in the corpus luteum. Testicular and ovarian sex cord tumors with annular tubules (SCTATs) associated with gynecomastia in prepubertal boys and isosexual precocity in girls with Peutz-Jeghers syndrome (P-JS) have been previously reported. In the present study, we investigated the regulatory elements directing P450arom gene transcription in samples of SCTAT from three prepubertal boys and a girl with P-JS and an ovarian granulosa cell tumor from an adult woman, as well as in healthy fetal and adult testicular and ovarian tissues. Placental tissue was used as a control. Using polymerase chain reaction linked to reverse transcription and northern blotting, we determined the tissue-specific use of various P450arom promoters by analyzing specific 5'-termini from messenger RNA templates. Results indicate a universal gonadal promoter (PII) directs P450arom gene expression in healthy fetal and adult ovaries and testes, as well as in SCTAT of the P-JS and an adult ovarian granulosa cell tumor. These results are interpreted to mean that use of PII in human ovary and testis is preserved from the fetal period into adult life as well as in transformed neoplastic Sertoli and granulosa cells. On the other hand, transcripts from placenta are specific for PI.1 (and to a much lesser extent, PI.2). In SCTAT, immunoreactive P450arom is detected only in the cytoplasm of neoplastic cells, whereas the normal-appearing sex cords do not contain any immunoreactive P450arom. These results further suggest that the markedly increased aromatase expression of these transformed neoplastic cells is not a consequence of using different tissue-specific promoters. Rather it appears to involve activation (or failure of inhibition) of the upstream regulatory elements of the same promoter, which is normally functional in all gonadal tissues, namely the proximal PII.
Assuntos
Aromatase/genética , Sistema Enzimático do Citocromo P-450/genética , Regulação Enzimológica da Expressão Gênica , Gônadas/enzimologia , Neoplasias Ovarianas/genética , Regiões Promotoras Genéticas/fisiologia , Tumores do Estroma Gonadal e dos Cordões Sexuais/genética , Neoplasias Testiculares/genética , Adolescente , Adulto , Aromatase/análise , Aromatase/metabolismo , Sequência de Bases , Northern Blotting , Criança , Pré-Escolar , Sistema Enzimático do Citocromo P-450/análise , Sistema Enzimático do Citocromo P-450/metabolismo , Feminino , Feto/metabolismo , Humanos , Imuno-Histoquímica , Masculino , Dados de Sequência Molecular , Neoplasias Ovarianas/enzimologia , Síndrome de Peutz-Jeghers/enzimologia , Síndrome de Peutz-Jeghers/genética , Reação em Cadeia da Polimerase , Gravidez , Regiões Promotoras Genéticas/genética , RNA Mensageiro/análise , RNA Mensageiro/genética , Tumores do Estroma Gonadal e dos Cordões Sexuais/enzimologia , Neoplasias Testiculares/enzimologia , Transcrição Gênica/genéticaRESUMO
We report the pathology findings in two cases of multicentric Sertoli cell testicular tumors in two young boys with probable Peutz-Jeghers syndrome. Four cases of such tumors occurring in boys with Peutz-Jeghers syndrome were previously reported. Each of the two boys reported in this paper had prominent gynecomastia, rapid growth, and advanced bone age. Serum levels of estradiol were markedly elevated. Anti-müllerian hormone was measured in the serum of one of the boys and was in the normal range for age. Bilateral orchiectomy was performed in each case because the neoplastic growth would most likely result in sterility, and curtailment of height potential was threatened from continued elevation of estradiol levels. Microscopically, greatly enlarged seminiferous tubules packed with ovoid Sertoli-like cells were present. Prominent eosinophilic basement membrane surrounded the tubules and intersected between the cells, forming hyalinized ovoid globules and microcalcifications. Ultrastructure revealed lamination of basement membranes surrounding adjacent cells, ovoid cells with abundant cytoplasm, and limited smooth endoplasmic reticulum. Studies of testicular tumor tissue from both cases revealed increased transcription of the aromatase cytochrome P450 gene using promoter II, the promoter directing aromatase expression in the normal ovary and testis. The levels of transcripts were comparable to corpus luteum, thus resulting in increased estrogen synthesis. Transcripts specific for placental-type aromatase promoters (I.1 and I.2) were not detected in significant levels in these tumors.
Assuntos
Feminização , Glicoproteínas , Síndrome de Peutz-Jeghers/complicações , Tumor de Células de Sertoli/complicações , Tumor de Células de Sertoli/patologia , Neoplasias Testiculares/complicações , Neoplasias Testiculares/patologia , Hormônio Antimülleriano , Pré-Escolar , Inibidores do Crescimento/metabolismo , Humanos , Masculino , Microscopia Eletrônica , Túbulos Seminíferos/patologia , Tumor de Células de Sertoli/metabolismo , Hormônios Testiculares/metabolismo , Neoplasias Testiculares/metabolismo , Testículo/patologiaRESUMO
We report on a family with transmission of a ring chromosome 14 from an affected mother to her 2 sons. The mother was mosaic, 46,XX,r(14)/45,XX,t(14q21q). Both of her sons, affected by seizures and mental retardation, have the karyotype 46,XY,r(14). In considering the association of translocation 14:21 in the mother with ring 14, we postulate that either the ring chromosome was formed first and then opened with translocation of the partially deleted chromosome 14 to chromosome 21, or the 14:21 translocation was present first, then the chromosomes 14 and 21 broke apart, and the partially deleted 14 formed the ring. The published literature of cases of ring 14 is reviewed.
Assuntos
Aberrações Cromossômicas , Cromossomos Humanos Par 14 , Translocação Genética , Adulto , Pré-Escolar , Bandeamento Cromossômico , Cromossomos Humanos Par 21 , Feminino , Humanos , Deficiência Intelectual , Cariotipagem , Masculino , Mosaicismo , SíndromeRESUMO
A study was made of the possible association of double nucleolus organizers (dNORs) with Turner syndrome. Occurrence of dNORs was determined using the silver staining method of Goodpasture and Bloom [1975]. In 45 control subjects, the incidence of dNORs was 8.8%. Studies were done on 33 Turner syndrome patients. In 28 cases with 45,X or 45,X/46,XX karyotypes, the incidence of the dNOR variant was 50%. Five cases of Turner syndrome with X rings or X isochromosomes were negative for dNORs. Analysis of the data indicates an association between the dNOR variant and the occurrence of Turner syndrome. It is proposed that the presence of the dNOR variant can increase the rate of nondisjunction of the X chromosome in meiosis or in mitotic divisions during early gestation.
Assuntos
Região Organizadora do Nucléolo/patologia , Síndrome de Turner/genética , Aberrações Cromossômicas , Feminino , Variação Genética , Humanos , Meiose , MitoseRESUMO
Ullrich-Turner syndrome occurred in one of a pair of female twins. The chromosome constitution of the affected twin was 45,X/46,XX and that of the normal twin 46,XX. Investigation of banded chromosomes, red cell antigens, HLA types, red cell enzymes, and serum proteins indicates monozygosity. The twins are discordant for height, pterygium colli, ovarian function, strabismus, dental eruption, external ear formation, hearing loss, and performance scores on the Wechsler Intelligence Test. All of these differences can be attributed to X monosomy in one cell line in the affected twin, presumably resulting from mitotic nondisjunction or anaphase lag early during embryonic development. Ten other pairs of apparently monozygotic twins discordant for the Ullrich-Turner syndrome have been reported previously, and the findings in these cases are reviewed.
Assuntos
Doenças em Gêmeos , Síndrome de Noonan/genética , Adolescente , Cefalometria , Deleção Cromossômica , Dentição , Feminino , Crescimento , Humanos , Inteligência , Cariotipagem , Não Disjunção Genética , Fenótipo , Gravidez , Gêmeos Monozigóticos , Cromossomo XRESUMO
A 26-year-old man having multiple endocrinopathy (pernicious anemia, hypothyroidism, hypoadrenocorticism, gonadal failure, and diabetes mellitus) and chronic candidiasis developed several rapidly growing primary tumors on the oral mucosa. Histologically, the tumors appeared to be very well differentiated squamous cell carcinomas. Yet, in spite of all therapeutic attempts, the tumors rapidly progressed and within eight months resulted in disseminated carcinomatosis and death. At autopsy the patient was found to have had a miniscule dysplastic thymus. It is postulated that in chronic candidiasis and polyendocrinopathy a defect may exist in immunologic cellular surveillance for recognition and destruction of aberrant cells.
Assuntos
Candidíase Bucal/complicações , Carcinoma de Células Escamosas/complicações , Doenças do Sistema Endócrino/complicações , Síndromes de Imunodeficiência/complicações , Neoplasias Bucais/complicações , Adulto , Autopsia , Candidíase Cutânea/complicações , Candidíase Cutânea/imunologia , Candidíase Bucal/imunologia , Carcinoma de Células Escamosas/mortalidade , Carcinoma de Células Escamosas/patologia , Humanos , Imunidade Celular , Masculino , Neoplasias Bucais/mortalidade , Neoplasias Bucais/patologia , Metástase Neoplásica , Síndrome , Timo/imunologiaRESUMO
The psychological development of females with congenital adrenal hyperplasia (CAH) has been previously studied by Money, et al, who found that psychological development of sex identity was consistent with sex assignment despite virilizing adrenal hormones and abnormal external genitalia requiring surgical correction. In this study, using a variety of psychological tests, we assessed the sex-dimorphic behavior, body image, cognitive functioning, and sex-role identity of nine patients ranging in age from 13 to 21, all treated with glucocorticoids and surgical correction. Four of the nine showed moderate virilization despite treatment. Psychological measures included the Wechsler Intelligence Scale for Children-Revised (WISC-R), the Bem Sex-Role Inventory, the Draw-A-Person (DAP) and an interview with patient and family. Results indicate that patients fall within the normal expectable range for this developmental period in visual-spatial and verbal cognitive functioning, in sex-role identity, and in social interpersonal early behaviors. In two areas of functioning these patients demonstrated some variance from the norms, specifically in sexual identity and early activity levels. This suggests that sexual identity and physical activity are most prone to hormonal and psychological impact but that cognition and sex-role identity are not affected. Future studies of this sample will look at personality dimensions such as ego functioning, defense and affect to consider the impact of body image concerns and conflicts.
Assuntos
Hiperplasia Suprarrenal Congênita/psicologia , Adolescente , Corticosteroides/uso terapêutico , Hiperplasia Suprarrenal Congênita/terapia , Adulto , Imagem Corporal , Clitóris/cirurgia , Cognição , Terapia Combinada , Feminino , Identidade de Gênero , Humanos , Testes de Inteligência , Entrevista Psicológica , Testes Psicológicos , Virilismo/psicologia , Virilismo/terapiaRESUMO
Persistent müllerian duct syndrome (PMDS) is characterized by the presence of a uterus, cervix, and fallopian tubes in an otherwise normally differentiated 46.XY male. During embryogenesis, regression of müllerian structures in normal males is mediated by antimüllerian hormone (AMH), also called müllerian inhibiting substance (MIS), produced by fetal Sertoli's cells. PMDS has been attributed to deficient AMH activity or to abnormalities in the AMH receptor. The authors report on two patients with PMDS in whom the abnormalities were discovered during surgery for inguinal hernia and cryptorchidism. During the initial operations in each case, testicular biopsies were obtained, and the gonads and müllerian elements were replaced in the pelvis. A second operative procedure, performed several months later, included proximal salpingectomies with dissection of the vasa deferentia on pedicles of myometrium. This permitted excision of the vestigial uterine corpus, leaving a tiny remnant of cervix with the vasa deferentia. The testes were further mobilized so that bilateral orchidopexies could be completed. In the first case, a molecular abnormality was present at position 377 of the first exon of the AMH gene. Thymine replaced cytosine, which altered a CGG arginine codon to a TGG tryptophan codon, rendering the AMH molecule unstable. The molecular abnormality in the first case differs from the first abnormality in AMH reported by Knebelmann et al, thus indicating heterogeneity in this condition. The molecular basis for deficient AMH activity in the second patient has not yet been defined. No molecular abnormalities were found in the exons of this patient's AMH gene.
Assuntos
Transtornos do Desenvolvimento Sexual/genética , Transtornos do Desenvolvimento Sexual/cirurgia , Glicoproteínas , Inibidores do Crescimento/genética , Ductos Paramesonéfricos , Hormônios Testiculares/genética , Hormônio Antimülleriano , Códon , Criptorquidismo/cirurgia , Transtornos do Desenvolvimento Sexual/diagnóstico , Hérnia Inguinal/cirurgia , Humanos , Lactente , Masculino , MétodosRESUMO
The long-term effects of severe hypothyroidism on craniofacial growth and dental development are illustrated in this case. It is apparent that given a favorable diet, the primary dentition can persist for a long period (early childhood to at least the age of 19) without the development of dental caries. It is also clear that the dental structures can still respond to the effects of L-thyroxine at a relatively late age, with the exfoliation of primary dentition and eruption of the secondary dentition. Impacted mandibular second molars appear to be rare. The lack of proper growth of the mandible and failure of normal resorption of the internal aspect of the ramus associated with deposition of bone on the external aspect with the development of normal-size teeth, resulted in a lack of space for the eruption of mandibular second molars. The impaction of the mandibular second molars in this patient seems to be caused by a dissociation of ramus growth and dental development, resulting in insufficient space for proper eruption of these teeth.
Assuntos
Hipotireoidismo Congênito , Ossos Faciais/crescimento & desenvolvimento , Crânio/crescimento & desenvolvimento , Erupção Dentária , Adulto , Cefalometria , Humanos , Hipotireoidismo/fisiopatologia , Masculino , Odontogênese , Dente Decíduo/fisiologiaRESUMO
The dental findings in three patients with hepatorenal glycogen storage disease are described. In two cases, dental maturation and tooth eruption were considerably delayed. In the third case, dental maturation and tooth eruption are normal, possibly because of newer treatment methods of this disease.