Biaxial Piezoelectric MEMS Mirrors with Low Absorption Coating for 1550 nm Long-Range LIDAR.

This paper presents the fabrication and characterization of a biaxial MEMS (MicroElectroMechanical System) scanner based on PZT (Lead Zirconate Titanate) which incorporates a low-absorption dielectric multilayer coating, i.e., a Bragg reflector. These 2 mm square MEMS mirrors, developed on 8-inch silicon wafers using VLSI (Very Large Scale Integration) technology are intended for long-range (>100 m) LIDAR (LIght Detection And Ranging) applications using a 2 W (average power) pulsed laser at 1550 nm. For this laser power, the use of a standard metal reflector leads to damaging overheating. To solve this problem, we have developed and optimised a physical sputtering (PVD) Bragg reflector deposition process compatible with our sol-gel piezoelectric motor. Experimental absorption measurements, performed at 1550 nm and show up to 24 times lower incident power absorption than the best metallic reflective coating (Au). Furthermore, we validated that the characteristics of the PZT, as well as the performance of the Bragg mirrors in terms of optical scanning angles, were identical to those of the Au reflector. These results open up the possibility of increasing the laser power beyond 2W for LIDAR applications or other applications requiring high optical power. Finally, a packaged 2D scanner was integrated into a LIDAR system and three-dimensional point cloud images were obtained, demonstrating the scanning stability and operability of these 2D MEMS mirrors.

Epidermal growth factor receptor in corneal damage: update and new insights from recent reports.

Agonist and antagonist drugs acting on epidermal growth factor receptor (EGFR) signaling are emerging as a new possibility for pharmaceutical study and clinical manipulation of some skin and corneal disorders. EGFR activation appears to be effective in reducing the time of reepithelialization after corneal wound healing, with potential uses in penetrating keratoplasty, refractive surgery, alkali burns, diabetic keratopathy, keratopathy following chemotherapy, cornea transplantation, and dry eye. Most of the studies show therapeutic advantages of human recombinant epidermal growth factor (hrEGF) eye drops without showing adverse effects. In contrast, EGFR inhibition delays epithelial cell proliferation and stratification during corneal regeneration.The aim of this review is to summarize the most seminal discoveries and recent advances so as to clarify the role of the EGFR system in corneal physiology and pharmacology. Epidermal growth factor eye drops could be a first-choice treatment for promoting regeneration in numerous epithelial defects in the medium to long term.

Adaptive self-mixing vibrometer based on a liquid lens.

A self-mixing laser diode vibrometer including an adaptive optical element in the form of a liquid lens (LL) has been implemented and its benefits demonstrated. The LL arrangement is able to control the feedback level of the self-mixing phenomenon, keeping it in the moderate feedback regime, particularly suitable for displacement measurements. This control capability has enabled a remarkable increase in the sensor-to-target distance range where measurements are feasible. Target vibration signal reconstructions present a maximum error of lambda radical16 as compared with a commercial sensor, thus providing an improved working range of 6.5 cm to 265 cm.

VCD studies on cyclic peptides assembled from L-α-amino acids and a trans-2-aminocyclopentane- or trans-2-aminocyclohexane carboxylic acid.

The increasing interest in peptidomimetics of biological relevance prompted us to synthesize a series of cyclic peptides comprising trans-2-aminocyclohexane carboxylic acid (Achc) or trans-2-aminocyclopentane carboxylic acid (Acpc). NMR experiments in combination with MD calculations were performed to investigate the three-dimensional structure of the cyclic peptides. These data were compared to the conformational information obtained by electronic circular dichroism (ECD) and vibrational circular dichroism (VCD) spectroscopy. Experimental VCD spectra were compared to theoretical VCD spectra computed quantum chemically at B3LYP/6-31G(d) density functional theory (DFT) level. The good agreement between the structural features derived from the VCD spectra and the NMR-based structures underlines the applicability of VCD in studying the conformation of small cyclic peptides.

Analysis of postfire vegetation dynamics of Mediterranean shrub species based on terrestrial and NDVI data.

The present study offers an analysis of regeneration patterns and diversity dynamics after a wildfire, which occurred in 1993 and affected about 7000 ha in southern Spain. The aim of the work was to analyze the rule in the succession of shrub species after fire, relating it to the changes registered in the Normalized Difference Vegetation Index (NDVI). Fractional vegetation cover was recorded from permanent plots in 2000 and 2005. NDVI data related to each time were obtained from Landsat images. Both data sets, from fieldwork and remote sensing, were analyzed through statistical and quantitative analyses and then correlated. Results have permitted the description of the change in plant cover and species composition on a global and plot scale. It can be affirmed that, from the seventh to the twelfth year after the fire, the floristic composition within the burned area remained unchanged at a global level. However, on a smaller scale (plot level), the major shrub species, Ulex parviflorus, Rosmarinus officinalis, and Cistus clusii, underwent significant changes. The regeneration dynamics established by these species conditioned plant species composition and, consequently, diversity indexes such as Shannon (H) and Simpson (D). The changes recorded in the NDVI values corresponding to the surveyed plots were highly correlated with those found in the regrowth of the main species. Areas dominated by U. parviflorus in a senile phase were related to a decrease in NDVI values and an increase in the number of species. This result describes the successional dynamics; the dryness of the main colonizer shrub species is allowing the regrowth and re-establishment of other species. Within the study area, NDVI shows sensitivity to postfire plant cover changes and indirectly expresses the diversity dynamics.

[Efficacy and safety of intravitreal injection of bevacizumab in the treatment of neovascular glaucoma: systematic review]. / Eficacia y seguridad de la inyección intravítrea de bevacizumab en el tratamiento del glaucoma neovascular: revisión sistemática.

PURPOSE: Systematic review on efficacy and safety of intravitreal bevacizumab (IVB) in the treatment of neovascular glaucoma (NVG). All original papers published in Medline (prior to August 2008) were included. METHODS: Search and selection of information on the internet and in Medline, validated by Kappa Index (K). Statistical and clinical study of the results in the selected articles on a one by one basis. RESULTS: 26 original papers analyzed the efficacy and safety of the procedure in case reports and short series of cases (127 eyes). The efficacy calculated in the sample was 68.7% and the recurrence rate was 18.6% in 4.2 months of follow-up. All studies were after 2006 and none of them was a clinical randomized controlled assay. Ophthalmic complications were under 0.78% and no systemic complications were found. CONCLUSIONS: The use of bevacizumab demonstrates that intravitreal injections may be effective and useful to manipulate growth factors in the anterior chamber. IVB could serve as a first line treatment for NVG. Clinical trials are needed to confirm these results before its use is authorized.

Evaluation of several fluoroquinolones and beta-lactams in terms of their capability to restrict the selection of fluoroquinolone-resistant Salmonella: in vitro models.

With a view to understanding the interaction between Salmonella and the drugs used to treat it, our aim was to compare the different capacities of various antibiotics to generate mutants resistant to fluoroquinolones following repeated exposure of the microorganisms to subinhibitory concentrations of these drugs. Mutants were generated by repeated exposure to fluoroquinolones and beta-lactams. In order to compare the different capacity to generate resistant mutants, we studied the minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) of the wild-type strains and of the mutants generated. These data were compared with pharmacokinetic parameters. Mutants generated following repeated exposure to fluoroquinolones exhibit an increased MPC as compared to the wild-type strains, both in strains that are nalidixic acid susceptible and in those that are nalidixic acid resistant, with repeated exposure to ciprofloxacin leading to the smallest increases. This increase in MPC is gradual and depends on the number of exposures the bacteria are subjected to. It results in a decrease in the AUC/MPC ratio, although the absolute values vary. Ciprofloxacin is the most active drug, both against nalidixic acid-susceptible and nalidixic acid-resistant strains, although in late mutants of originally nalidixic acid-resistant strains, the AUC/MPC values are low. Repeated exposure to amoxicillin and cefotaxime also produces an increase in the MPC of fluoroquinolones, with ciprofloxacin being the least affected. Exposure to amoxicillin leads to the greatest increase in the MPC of fluoroquinolones. When the AUC/MPC ratios of these mutants are compared, the values are still seen to be high (between 25 and 75). When we compare the MPC data with the antibiotic levels in humans following administration of the usual doses, it can be seen that ciprofloxacin exhibits the highest AUC/MPC and therefore the lowest risk of therapeutic failures. In addition, administration of subinhibitory concentrations of beta-lactams produces a decrease in fluoroquinolone susceptibility, which may lead to an increase in the risk of therapeutic failure if these compounds are subsequently used.

Multi-channel receptors based on thiopyrylium functionalised with macrocyclic receptors for the recognition of transition metal cations and anions.

We report herein the synthesis and characterization of a family of ligands containing different cation binding sites covalently connected to a thiopyrylium signalling reporter. The receptors L1-L6 are able to signal the presence of certain metal cations via three different channels; i.e. electrochemically, fluorogenically and chromogenically. An acetonitrile solution of L1-L6 shows a bright blue colour due to a charge-transfer band in the 575-585 nm region. The colour variation in acetonitrile of L1-L6 in the presence of the metal cations Ag+, Cd2+, Cu2+, Fe3+, Hg2+, Ni2+, Pb2+ and Zn2+ has been studied. A selective hypsochromic shift of the blue band was found for the systems L4-Pb2+ and L5-Hg2+. Additionally, L1-L6 are poorly fluorescent but coordination with certain metal cations induces an enhancement of the fluorescence at ca 500 nm. For instance, the presence of Cu2+ and Fe3+ induced a remarkable 42-fold and 45-fold enhancement in the emission intensity of L1 centred at 500 nm, respectively. Also remarkable was the 18-fold enhancement observed for L4 and L5 in the presence of Fe3+ and Cu2+, respectively. The electrochemical behaviour of receptors L1-L6 was studied in acetonitrile using platinum as a working electrode and [Bu4N][BF4] as a supporting electrolyte. This family of receptors showed a one-electron reversible redox process at ca. -0.46 V versus sce attributed to the reduction of the thiopyrylium group. A moderate anodic shift in the presence of certain metal cations was observed. The effect in the UV-visible spectra of acetonitrile solutions of receptor L1-L6 in the presence of anions was also studied. A remarkable bleaching was found in the presence of cyanide.

Shack-Hartmann sensor based on a cylindrical microlens array.

We present a Shack-Hartmann wavefront sensor (SHWS) based on a cylindrical microlens array as a device for measuring highly aberrated wavefronts. Instead of the typical spot pattern created by a conventional SHWS, two orthogonal line patterns are detected on a CCD and are superimposed. A processing algorithm uses the continuity of the focal line to extend the dynamic range of measurement by localizing the line, even if it leaves the CCD area confined by the corresponding microcylinder. The measurement of a wavefront from a progressive addition lens with an 80 lambda peak-to-valley value reveals the capabilities of the sensor.

Comparison of cubic B-spline and Zernike-fitting techniques in complex wavefront reconstruction.

We analyze an alternative to classical Zernike fitting based on the cubic B-spline model, and compare the strengths and weaknesses of each representation over a set of different wavefronts that cover a wide range of shape complexity. The results obtained show that a Zernike low-degree polynomial expansion or a cubic B-spline with a low number of breakpoints are the best choices for fitting simple wavefronts, whereas the cubic B-spline approach performs much better when more complex wavefronts are involved. The effect of noise level in the fit quality for the different wavefronts is also studied.

Simple method for improving the sampling in profile measurements by use of the Ronchi test.

We present a simple method for increasing the number of data points obtained during performance of profilometric measurements with the Ronchi test. The method is based on multiple ronchigram acquisitions that are superimposed after a few very simple data-processing operations. The measurement method, experimental setup, and data processing are described in detail from the ronchigram to the measured profile, and experimental results for a concave surface of an spherical ophthalmic lens are provided. The radius of curvature values measured for that surface are compared with the ones obtained with a high-precision radioscope, showing very good agreement and demonstrating the capability of the technique to measure topographic profiles of reflective samples.

Profilometry of toroidal surfaces with an improved ronchi test.

An implementation of the well-known Ronchi test technique, which allows for the profilometric measurement of nonrotationally symmetrical surfaces, is presented and applied to the measurement of toroidal surfaces. Both the experimental setup and the data-processing procedures are described, and parameters such as the radius of curvature of the sample surface, the orientation of its principal meridians, and the position of its vertex are measured by means of the values of the local normal to the surface obtained at a set of sampling points. Integration of these local normal values allows for the reconstruction of the three-dimensional profile of the toroidal surface considered with micrometric accuracy, and submicrometric surface details may be calculated by use of surface-fitting procedures. The density of sampling points on the surface may be tailored to fit test requirements, within certain limits that depend on selection of experimental setup.

Flux spatial emission obtained from technical specifications for a general filament light source.

A new, to our knowledge, method for evaluating three-dimensional flux distributions for general filament light sources is presented. The main advantages of the developed model are its generality and its simplicity. From plots of the emitted luminous intensity, usually provided by the lamp's manufacturer, in three orthogonal planes a detailed account is given of how to establish flux emission from the light source in any direction. The method involves a selective smoothing procedure, a curve-fitting step, and a final interpolation. A full model is developed for a typical commercial filament bulb (Philips, Model P21W Inco K) that is quite common in many industrial applications. A fourth intensity plot, usually provided by the lamp's manufacturer, is used to validate the model. To confirm the validity of the model further, we present an industrial application (the photometric simulation of a car taillight) that uses the modeled Philips Model P21W source. A comparison between simulated data obtained by use of the developed P21W model and measured results at our industrial partner's laboratories reinforces the proposed source model.