RESUMO
A squash technique was developed for log phase Tetrahymena pyriformis which permitted the resolution of over 100 individual mitochondria from a single cell. Mitochondria incorporated thymidine at all stages of the cell cycle, even when nuclear DNA synthesis was not occurring. During the stage of macronuclear DNA synthesis, however, there was a significant increase in the extent of mitochondrial labeling. Low radioautograph background suggests that mitochondrial DNA is synthesized at the mitochondria themselves. All mitochondria incorporated thymidine-3H within one population-doubling time. Grain counts also showed that the amount of mitochondrial label was retained for four generations and that this label remained randomly distributed among all mitochondria during this time. The results are not consistent with any theory of de-novo or "microbody" origin of mitochondria, but do support the hypothesis that mitochondria are produced by the growth and division of preexisting mitochondria. The stability of the mitochondrial DNA and its distribution among daughter mitochondria satisfy two prerequisites for a genetic material. The possibility is discussed that some of the genetic information for the mitochondrion is contained in the DNA associated with this organelle.
Assuntos
Núcleo Celular/metabolismo , DNA Mitocondrial/fisiologia , DNA Mitocondrial/ultraestrutura , DNA/biossíntese , Mitocôndrias/ultraestrutura , Tetrahymena pyriformis/metabolismo , Animais , Ciclo Celular , Divisão Celular , Microscopia de Contraste de Fase , Fatores de TempoRESUMO
Sea urchin eggs were cut into halves. The nucleate and anucleate halves and whole eggs were irradiated with gamma-rays and then fertilized with normal sperm. The first mitosis of the diploid half-egg was more delayed than the division of the whole egg. There was a small, but highly significant, delay of the mitosis of the haploid half-egg, thus demonstrating cytoplasmic sensitivity to ionizing radiation. Since the sensitivity of nucleate cells is influenced by cytoplasmic volume, the problem of the role of cytoplasm in repair is considered in relation to these data and other reports in the literature.
Assuntos
Núcleo Celular/efeitos da radiação , Citoplasma/efeitos da radiação , Mitose/efeitos da radiação , Óvulo/efeitos da radiação , Efeitos da Radiação , Animais , Diploide , Equinodermos/efeitos da radiação , Feminino , Fertilização , Haploidia , MétodosRESUMO
Sublethal doses of gamma radiation are known to inhibit total RNA synthesis in the ciliate protozoan Tetrahymena. To determine if the synthesis of a particular class of RNA is preferentially inhibited, pulse-labeled RNA was isolated from normal exponentially growing cells, irradiated cells, and cells in which total RNA synthesis had recovered to the pre-irradiation level. The RNAs were analyzed by SDS-polyacrylamide gel electrophoresis and oligo(dT)-cellulose column chromatography. Inhibition of RNA synthesis primarily involves ribosomal RNA. However, radiation does not cause a delay in the processing of precursor rRNA or a preferential loss of either of the mature rRNAs. Following irradiation, poly(A)-containing RNA [poly(A+)RNA] is synthesized at a rate up to three times greater than the control rate. The elevated poly(A+)RNA synthesis occurs during the period of depressed rRNA synthesis and even after rRNA synthesis has recovered to its pre-irradiation rate. While the sizes of the total cellular ribonucleoside triphosphate pools are depressed in the irradiated cells, these pools probably do not represent the actual compartments containing the precursors for RNA synthesis, and the observed changes cannot explain the modifications in macromolecular synthesis in irradiated Tetrahymena.
Assuntos
Precursores de Ácido Nucleico/metabolismo , RNA Ribossômico/efeitos da radiação , Tetrahymena pyriformis/efeitos da radiação , Raios gama , RNA/biossíntese , RNA Ribossômico/biossíntese , Tetrahymena pyriformis/metabolismoAssuntos
Polirribossomos/efeitos da radiação , Efeitos da Radiação , Tetrahymena pyriformis/efeitos da radiação , Animais , Sítios de Ligação , Centrifugação com Gradiente de Concentração , Radioisótopos de Cobalto , Temperatura Baixa , Cicloeximida , Detergentes , Estudos de Avaliação como Assunto , Leucina/metabolismo , Substâncias Macromoleculares , Métodos , Polirribossomos/efeitos dos fármacos , Polirribossomos/metabolismo , Biossíntese de Proteínas/efeitos da radiação , Puromicina/farmacologia , Espectrofotometria Ultravioleta , Tetrahymena pyriformis/citologia , Tetrahymena pyriformis/efeitos dos fármacos , Tetrahymena pyriformis/metabolismo , Fatores de Tempo , TrítioRESUMO
The polysome content of the ciliate protozoan Tetrahymena pyriformis is transiently reduced by gamma-irradiation. In order to test whether this is a result of a respiration-produced or radiation-produced hypoxia or some other mechanism, the oxygen content of the culture was determined during and after irradiation, and the polysome contents and rates of amino acid incorporation were measured with and without air bubbling. Irradiation (40 krad at approximately 3 krad/min) produced approximately a 25 per cent loss in dissolved O2 content in the medium. This decrease is not sufficient to affect the polysome level, since (a) the same radiation-induced loss of polysomes and inhibiition of amino acid incorporation was observed whether or not the culture was bubbled with air during the irradiation and (b) bubbling unirradiated cultures with gas mixtures containing as little as 17 per cent of the normal O2 content did not influence the polysome level. As long as the cells are irradiated as a shallow layer in open flasks, replacement of O2 from the gas phase appears adequate, and neither respiration-induced nor radiation-induced hypoxia masks the effects of the radiation.
Assuntos
Consumo de Oxigênio , Polirribossomos/metabolismo , Tetrahymena pyriformis/efeitos da radiação , Aminoácidos/metabolismo , Raios gama , Oxigênio/análise , Polirribossomos/análise , Biossíntese de Proteínas , Tetrahymena pyriformis/metabolismo , Tetrahymena pyriformis/ultraestruturaRESUMO
Radiation-induced disturbances in RNA synthesis were investigated in exponentially growing Tetrahymena. Sub-lethal doses of gamma-radiation lead to a transient, dose-dependent decrease in the rate of total RNA synthesis measured by 3H-uridine incorporation, without an alteration of 3H-uridine uptake by the cells. The rate of 3H-uridine incorporation decreases exponentially with dose. In contrast, the duration of inhibition of RNA synthesis is linearly dependent on dose. Target-theory calculations suggest that the sensitive molecule has a molecular weight of about 2 X 10(7) Daltons.