RESUMO
Fluorescent calcium indicators have been widely used to assess cytoplasmic calcium concentration in cells. To examine the role of calcium ions on different physiological functions (e.g. in case of liver; bile secretion, glucose metabolism, etc.) there is a need for whole organ studies. We have developed a technique to estimate intracellular free calcium changes in perfused rat liver. Krebs-Henseleit perfused livers were loaded with 7 microM or 35 microM Indo-1/AM. An area 3 mm in diameter and approximately 300 microns in depth was illuminated at 340 nm. Fluorescence was monitored with photomultiplier tubes at 3 wavelengths (400 nm for Ca-bound dye, 504 nm for free dye and 464 nm for NADH). The viability of liver preparations was assessed by measurement of the concentrations of lactate dehydrogenase and alanine aminotransferase in the effluent. Loading of the livers with 7 microM Indo-1/AM via the portal vein resulted in a 5-fold increase of fluorescence at 400 nm. However the dye 'leaked' out of the liver with a half-time of 18 min. Probenecid (a specific anion carrier blocker) inhibited loss of dye in a dose dependent fashion (2.5-10 mM). Transient calcium elevations were observed in response to vasopressin (5-50 nM) at physiological levels, ethanol (0.3-0.8 M) and the calcium ionophore, ionomycin. Certain limitations were apparent with this approach: (1) it was necessary to use an anion carrier blocker to maintain a relatively steady dye concentration; (2) endogenous NADH fluorescence interfered with the calcium signal; and (3) absolute values of calcium concentration could not be determined.
Assuntos
Cálcio/metabolismo , Corantes Fluorescentes/metabolismo , Indóis/metabolismo , Líquido Intracelular/metabolismo , Fígado/metabolismo , Animais , Proteínas de Transporte de Ânions , Artefatos , Proteínas de Transporte/antagonistas & inibidores , Permeabilidade da Membrana Celular/efeitos dos fármacos , Etanol/farmacologia , Fluorometria , Ionomicina/farmacologia , Masculino , NAD/metabolismo , Perfusão , Probenecid/farmacologia , Ratos , Ratos Wistar/metabolismo , Sensibilidade e Especificidade , Vasopressinas/farmacologiaRESUMO
Indo-1 fluorescence was used to monitor intracellular calcium levels in the cat brain in vivo, using the approach proposed by Uematsu et al. [Uematsu D., Greenberg J. H., Reivich M., Karp A. In vivo measurement of cytosolic free calcium during cerebral ischemia and reperfusion. Ann Neurol 1988; 24: 420-428]. In addition, extracellular calcium and potassium levels, NADH redox state, electrocorticogram (ECoG), DC potential and relative cerebral blood flow were monitored simultaneously. Changes in the Indo-1 fluorescence ratio F400/F506 were monitored during anoxia, reversible ischemia and irreversible ischemia. Although these perturbations resulted in the expected changes in extracellular calcium and potassium levels, NADH redox state, ECoG and other physiological parameters, they did not result in significant increases in the F400/F506 ratio. The apparent insensitivity of the in vivo Indo-1 approach is due to the difficulty in obtaining accurate fluorescence signals from Indo-1 in the brain. Two reasons for this difficulty appear to be problems in loading Indo-1 into the brain, and problems in correcting Indo-1 fluorescence signals for changes in NADH fluorescence and changes in absorption of intrinsic chromophores. Under the conditions of our in vivo cat experiments, Indo-1 fluorescence is not a viable approach for measuring changes in cerebral intracellular calcium levels.
Assuntos
Isquemia Encefálica/metabolismo , Cálcio/metabolismo , Corantes Fluorescentes , Hipóxia Encefálica/metabolismo , Indóis , Animais , Encéfalo/irrigação sanguínea , Encéfalo/metabolismo , Gatos , Circulação Cerebrovascular , Fluorometria/instrumentação , Fluorometria/métodos , Potenciais da Membrana , NAD/química , Oxirredução , Potássio/análiseRESUMO
The effect of the nitric oxide (NO) synthase inhibitor N omega-nitro-L-arginine methyl ester (L-NAME) on the response of cerebrocortical oxygen consumption (CMRO2) and blood flow (CBF) to two levels of hypercapnia (PaCO2 approximately 60 mm Hg and PaCO2 approximately 90 mm Hg) was investigated in ketamine-anesthetized rats. CBF was calculated using the Kety-Schmidt approach and CMRO2 was calculated from the product of CBF and the arteriovenous (superior sagittal sinus) difference for oxygen. L-NAME treatment did not have a significant effect on either CMRO2 or CBF under normocapnic conditions but inhibited the hypercapnic increase of CMRO2 and the hypercapnic increase in CBF. These results suggest that NO plays a role in the response of CMRO2 and CBF during hypercapnia and are consistent with the suggestion that at least part of the increase in CBF observed during hypercapnia is coupled to an increase in CMRO2.
Assuntos
Córtex Cerebral/metabolismo , Circulação Cerebrovascular , Hipercapnia/fisiopatologia , Óxido Nítrico/fisiologia , Consumo de Oxigênio , Animais , Arginina/análogos & derivados , Arginina/farmacologia , Pressão Sanguínea/efeitos dos fármacos , Córtex Cerebral/irrigação sanguínea , Circulação Cerebrovascular/efeitos dos fármacos , Masculino , NG-Nitroarginina Metil Éster , Óxido Nítrico/antagonistas & inibidores , RatosRESUMO
The effect of systematically delayed progesterone treatment on the onset of labour was examined in 45 pregnant rats. Measurement of progesterone (P) and prostaglandin F (PGF) in uterine vein plasma and uterine tissue before and during spontaneous labour or during prolonged pregnancy showed that the control animals exhibited the expected P-withdrawal (Pw) prior to spontaneous labour, however, properly timed P-treatment predictably prevented Pw and labour. When P was administered 11.7 +/- 2.8 hours before spontaneous labour, the animals delivered normally, despite increased plasma and tissue P-levels. These observations show that P-concentration cannot be equated to P-action.
Assuntos
Início do Trabalho de Parto/efeitos dos fármacos , Trabalho de Parto/efeitos dos fármacos , Progesterona/administração & dosagem , Animais , Feminino , Gravidez , Progesterona/sangue , Progesterona/metabolismo , Progesterona/farmacologia , Prostaglandinas F/sangue , Prostaglandinas F/metabolismo , Ratos , Ratos Endogâmicos , Fatores de Tempo , Útero/irrigação sanguínea , Útero/metabolismo , VeiasRESUMO
The effect of systematically delayed progesterone treatment was examined in 45 pregnant rats near term. Progesterone (P) and prostaglandin F (PGF) were measured in uterine vein plasma and uterine tissue before and during spontaneous labor or during prolonged pregnancy. Control animals exhibited the expected P-withdrawal (Pw) prior to spontaneous labor and properly timed P-treatment predictably prevented Pw and labor. However, when P was administered 11.7 +/- 2.8 hours (Mean +/- S.E.) before spontaneous labor, the animals delivered normally despite increased plasma and tissue P-levels. These observations show that P-concentration can not be equated to P-action. Thus, when high P-levels are measured near term, as in parturient women, the biological ACTION of this hormone on uterine function should be cautiously interpreted.
Assuntos
Trabalho de Parto/efeitos dos fármacos , Progesterona/sangue , Prostaglandinas F/sangue , Útero/fisiologia , Animais , Feminino , Gravidez , Progesterona/farmacologia , Ratos , Útero/irrigação sanguíneaRESUMO
Fine, varicose oxytocin-containing nerve fibers have been demonstrated in the hypothalamic arcuate nucleus in rats. Using Phaseolus vulgaris leukoagglutinin as an anterograde tracer, fine neuronal fibers of paraventricular nucleus origin could be seen throughout the arcuate nucleus. Using double immunostaining, oxytocin-immunoreactive varicose fibers were observed around or in the close vicinity of beta-endorphin-immunoreactive neurons. Silver-gold-labeled oxytocin-immunoreactive presynaptic boutons were shown to make synaptic contacts with diaminobenzidine-labeled beta-endorphin-immunoreactive neurons by electron microscopy. These findings provide morphological evidence for a possible influence of oxytocin on the activity of the brain beta-endorphin system at the hypothalamic level.
Assuntos
Núcleo Arqueado do Hipotálamo/química , Fibras Nervosas/química , Neurônios/química , Ocitocina/análise , beta-Endorfina/análise , Animais , Núcleo Arqueado do Hipotálamo/citologia , Feminino , Fixadores , Técnicas Imunoenzimáticas , Microscopia Eletrônica , Fito-Hemaglutininas , Ratos , Ratos Sprague-Dawley , Coloração e RotulagemRESUMO
Simultaneous 17O and 19F magnetic resonance imaging were used to determine the concentrations of H2(17)O and CHF3 in 0.8 cc voxels in the cat brain during in- and exhalation of a gas mixture containing both 17O2 and CHF3. The arterial time course of H2(17)O was determined by 17O MR analysis of arterial samples withdrawn during the inhalation period and the arterial time concentration of CHF3. The brain data and the arterial data for the two tracers were used to calculate the cerebral oxygen consumption (CMRO2) and the cerebral blood flow (CBF). The average values of CMRO2 and CBF for a 0.8 cc voxel in the parietal cortex were 1.5 +/- 0.5 mmol/kg/min and 38 +/- 15 ml/100g/min, respectively. 17O/19F MR imaging approach has the potential to image CMRO2 and CBF simultaneously in humans and might become a strong diagnostic tool.
Assuntos
Encéfalo/anatomia & histologia , Encéfalo/metabolismo , Circulação Cerebrovascular , Angiografia por Ressonância Magnética/métodos , Consumo de Oxigênio , Animais , Gatos , Artérias Cerebrais/anatomia & histologia , Flúor , Angiografia por Ressonância Magnética/instrumentação , Angiografia por Ressonância Magnética/estatística & dados numéricos , Isótopos de Oxigênio , Fatores de TempoRESUMO
The aim of this work was to develop a model to study the microcirculation and relative levels of intracellular free calcium in the myometrium of pregnant rats. On Day 21 of gestation a lobe of uterus was prepared free, flipped over, and mounted in a superfusion chamber leaving the radix and thereby the innervation and circulation intact. RBC velocity and arteriolar diameters were determined by means of intravital video microscopy before and after stimulation (norepinephrine). To study intracellular free calcium changes, the fluorescent dye Indo-1 AM was added to the superfusate in the chamber. Fluorescence images were recorded and ratios of the images collected at 400 and 506 nm were calculated and changes thereof were assumed to represent intracellular free calcium changes. RBC velocity and arteriolar diameter did not change for at least 1 h, while the response to norepinephrine was similar at the beginning of the experiment and after 120 min. In four separate interventions, the uterus was challenged with 5 x 10(-4) IU/ml oxytocin, 4.5 mM calcium, 5 x 10(-4) IU/ml oxytocin with 4.5 mM calcium, and 5 microM ionomycin, resulting in an increase of the 400/506 nm ratio of 27, 31, 76, and 103%, respectively, representing a relative increase in intracellular free calcium. This novel in vivo model is suitable for monitoring intracellular free calcium changes and to record RBC velocities and blood vessel diameters in the myometrium of pregnant rats.
Assuntos
Cálcio/metabolismo , Miométrio/irrigação sanguínea , Animais , Arteríolas/ultraestrutura , Velocidade do Fluxo Sanguíneo/efeitos dos fármacos , Feminino , Líquido Intracelular/metabolismo , Ionomicina/farmacologia , Ionóforos/farmacologia , Microcirculação/efeitos dos fármacos , Microscopia de Vídeo , Miométrio/efeitos dos fármacos , Miométrio/metabolismo , Norepinefrina/farmacologia , Ocitocina/farmacologia , Gravidez , Ratos , Ratos Sprague-DawleyRESUMO
Intracellular free Ca2+ concentration ([Ca2+]i) plays an essential role in physiological regulatory processes and common pathological conditions. Better understanding of these phenomena is still hampered by problems encountered in the quantitative assessment of [Ca2+]i changes, especially in blood-perfused organs. This study demonstrates that the ratiometric fluorescence technique can be adapted for quantitative in vivo [Ca2+]i determinations. The rat spinotrapezius muscle was topically loaded with indo 1-AM and imaged by a cooled digital camera. Ratio images were calculated in small regions (100 micrometers x 100 micrometers) practically devoid of large vessels in the resting state, after 30 min of ischemia, 20 min of reperfusion, or ionomycin or manganate treatments. When we assumed an average [Ca2+]i of 100 nM in the resting blood-perfused muscle, ischemia increased [Ca2+]i to approximately 200 nM. During reperfusion [Ca2+]i decreased to approximately 140 nM. Ionomycin induced an increase in [Ca2+]i to well above 750 nM. Manganate reduced Ca2+-dependent fluorescence to virtually zero. Our main conclusion is that changes in [Ca2+]i can be monitored and quantitatively determined in vivo.
Assuntos
Cálcio/metabolismo , Músculo Esquelético/metabolismo , Animais , Processamento de Imagem Assistida por Computador , Masculino , Microscopia de Fluorescência/métodos , Ratos , Ratos Sprague-DawleyRESUMO
Accumulation of intracellular free calcium (Ca2+i) may play an essential role in the ischemia/reperfusion injury of skeletal muscle. Although it has been shown that Ca2+i levels significantly increase during ischemia/reperfusion, it is still a matter of debate whether Ca2+i increases during ischemia alone. It was the aim of this study to monitor the in vivo Ca2+i levels in the rat spinotrapezius muscle during ischemia of varying duration and reperfusion, using a ratiometric fluorescence technique, and to investigate the relationship between the postischemic flow patterns and Ca2+i, if any. The muscle was loaded with Indo-1/AM and imaged by a cooled digital camera. Pre- and postischemic tissue perfusion was assessed by means of an analogue camera. Our results show that short-term ischemia (5, 15 and 30 min) and subsequent reperfusion (60 min) does not alter Ca2+i homeostasis and that tissue perfusion promptly recovers after the insult. One or two hours of ischemia resulted in changes in Ca2+i levels, varying from preparation to preparation; increases in some and no changes in others. In these preparations three distinct flow patterns - normal, compromised and no-reflow - could be distinguished during the 60-min reperfusion. Our main conclusion is that in skeletal muscle Ca2+i levels may increase, the increase probably depending on the muscle fiber type exposed.
Assuntos
Cálcio/metabolismo , Membranas Intracelulares/metabolismo , Isquemia/metabolismo , Fibras Musculares Esqueléticas/metabolismo , Músculo Esquelético/metabolismo , Traumatismo por Reperfusão/metabolismo , Animais , Corantes Fluorescentes , Indóis , Masculino , Ratos , Ratos Sprague-DawleyRESUMO
In vivo 17O NMR has been used to monitor the H2(17)O concentration in rat brain during inhalation of 17O2. The results are discussed in terms of oxygen consumption in the brain and recirculation into the brain of H2(17)O produced in other organs.
Assuntos
Encéfalo/metabolismo , Animais , Circulação Cerebrovascular/fisiologia , Espectroscopia de Ressonância Magnética , Masculino , Consumo de Oxigênio/fisiologia , Isótopos de Oxigênio , RatosRESUMO
We used 17O NMR imaging techniques to measure the H2(17)O concentration in a 0.8-ml voxel in the cat brain following injection of an arterial bolus of enriched H2(17)O and during inhalation of enriched 17O2. We also measured the H2(17)O concentration in arterial blood during 17O2 inhalation. The data from the first measurement were used to calculate the blood flow in the voxel. The data from all three measurements were combined to calculate the oxygen consumption in the voxel. The values of cerebral blood flow and oxygen consumption calculated with 17O NMR techniques agree reasonably well with values calculated for a similar region of the cat brain using autoradiographic techniques.