1.
Angew Chem Int Ed Engl
; 56(46): 14556-14560, 2017 11 13.
Artigo
em Inglês
| MEDLINE
| ID: mdl-28941028
RESUMO
We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.