RESUMO
Electron microscopical and electron histochemical studies and functional investigation of platelets have been undertaken in 10 normal individuals and 20 patients with ischaemic heart disease and angina pectoris of effort before and after acetylsalicylic acid (ASA) administration during 3 days. In ischaemic heart disease changes in the ultrastructure of platelets have been recorded, pointing to their activation. After ASA administration there was decrease of functional activity, due to structural changes in the plasma membrane, lysosome membranes, fragmentation of platelets, enhanced process of autophagia, decrease of enzymatic activity of structure organelles of platelets.
Assuntos
Aspirina/efeitos adversos , Plaquetas/efeitos dos fármacos , Doença das Coronárias/tratamento farmacológico , Adulto , Angina Pectoris/sangue , Angina Pectoris/tratamento farmacológico , Plaquetas/fisiologia , Plaquetas/ultraestrutura , Doença das Coronárias/sangue , Histocitoquímica , Humanos , Microscopia Eletrônica , Pessoa de Meia-Idade , Testes de Função PlaquetáriaAssuntos
Diagnóstico , Ética Médica , Pacientes , Humanos , Ciência de Laboratório Médico , Medicina , Relações Médico-Paciente , Radiografia , EspecializaçãoRESUMO
The purpose of this study was to compare the in vitro metabolic changes in mouse peritoneal macrophages exposed to sera of CHD patients and healthy donors. Oxidative (metabolic) burst was estimated by the activity of one of the limiting enzymes of hexose monophosphate shunt--glucoso-6-phosphate dehydrogenase. Pronounced enhancement of glucoso-6-phosphate dehydrogenase activity accompanied the exposure of macrophages to sera of patients with acute myocardial ischemia during the first two days after the onset of anginal pains. The correlation was established between the activity of creatine kinase in the sera of patients with acute myocardial infarction and the ability of these sera to enhance glucoso-6-phosphate dehydrogenase activity in mouse peritoneal macrophages.