RESUMO
Precise control of blood clotting and rapid reversal of anticoagulation are essential in many clinical situations. We were successful in modifying a thrombin-binding aptamer with a red-light photocleavable linker derived from Cy7 by Cu-catalyzed Click chemistry. We were able to show that we can successfully deactivate the modified aptamer with red light (660â nm) even in human blood-restoring the blood's natural coagulation capability.
Assuntos
Anticoagulantes/farmacologia , Benzotiazóis/farmacologia , Coagulação Sanguínea/efeitos dos fármacos , Carbocianinas/farmacologia , Luz , Anticoagulantes/química , Benzotiazóis/química , Carbocianinas/química , Humanos , Estrutura MolecularRESUMO
meso-methyl-BODIPY photocages release a leaving group upon visible light irradiation but often lack thermal stability. In turn, our thermally-stable, red-shifted BODIPY phototether allows oligonucleotide cyclization, preventing complementary strand hybridization. Hybridization resumes upon red-NIR irradiation, disconnecting the phototether by oxidative cleavage, which is easily monitored by a blue shift in fluorescence.