RESUMO
Pex, a clock-related protein involved in the input pathway of the cyanobacterial circadian clock system, suppresses the expression of clock gene kaiA and lengthens the circadian period. Here, we determined the crystal structure of Anabaena Pex (AnaPex; Anabaena sp. strain PCC 7120) and Synechococcus Pex (SynPex; Synechococcus sp. strain PCC 7942). Pex is a homodimer that forms a winged-helix structure. Using the DNase I protection and electrophoresis mobility shift assays on a Synechococcus kaiA upstream region, we identified a minimal 25-bp sequence that contained an imperfectly inverted repeat sequence as the Pex-binding sequence. Based on crystal structure, we predicted the amino acid residues essential for Pex's DNA-binding activity and examined the effects of various Ala-substitutions in the alpha3 helix and wing region of Pex on in vitro DNA-binding activity and in vivo rhythm functions. Mutant AnaPex proteins carrying a substitution in the wing region displayed no specific DNA-binding activity, whereas those carrying a substitution in the alpha3 helix did display specific binding activity. But the latter were less thermostable than wild-type AnaPex and their in vitro functions were defective. We concluded that Pex binds a kaiA upstream DNA sequence via its wing region and that its alpha3 helix is probably important to its stability.
Assuntos
Anabaena/metabolismo , Proteínas de Bactérias/química , Synechococcus/metabolismo , Transativadores/química , Sequência de Aminoácidos , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Sequência de Bases , Bioensaio , Proteínas CLOCK , Cristalografia por Raios X , DNA Bacteriano/metabolismo , Dimerização , Regulação Bacteriana da Expressão Gênica , Medições Luminescentes , Dados de Sequência Molecular , Proteínas Mutantes/química , Proteínas Mutantes/metabolismo , Ligação Proteica , Desnaturação Proteica , Estabilidade Proteica , Estrutura Secundária de Proteína , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Alinhamento de Sequência , Relação Estrutura-Atividade , Temperatura , Transativadores/genética , Transativadores/metabolismoRESUMO
Simultaneous determination of zircon U-Pb age and titanium concentration for a single analysis spot gives both the crystallization age and temperature. In laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) analysis, it is challenging to quantitatively analyse a low level of titanium concentration. Two approaches were employed using a quadrupole mass spectrometer equipped with a collision/reaction cell (CRC). In the first approach, the MS/MS mass-shift mode with oxygen reaction gas provided reliable and consistent measurement of titanium as 48Ti16O+. In the second approach, the titanium concentration was determined quantitatively from the signal intensity of 49Ti in the non-gas mode (without the inflow of collision/reaction gas into the CRC). The methods were applied to zircon samples of the Kurobegawa granite (KRG), the Okueyama granite (OKG), the Toki granite (TKG), and the Tono plutonic complex (TCP). The biotite K-Ar geochronology were employed for rock samples of the KRG, OKG, and TPC (Nâ¯=â¯3) of which the zircon crystals were analysed. The obtained titanium concentrations of the zircon crystals can lead to the crystallization temperatures through Ti-in-zircon geothermometer.
RESUMO
The chronology of the World Heritage Site of Sangiran in Indonesia is crucial for the understanding of human dispersals and settlement in Asia in the Early Pleistocene (before 780,000 years ago). It has been controversial, however, especially regarding the timing of the earliest hominin migration into the Sangiran region. We use a method of combining fission-track and uranium-lead dating and present key ages to calibrate the lower (older) Sangiran hominin-bearing horizons. We conclude that the first appearance datum for the Sangiran hominins is most likely ~1.3 million years ago and less than 1.5 million years ago, which is markedly later than the dates that have been widely accepted for the past two decades.
Assuntos
Evolução Biológica , Hominidae , Animais , Antropologia , Humanos , IndonésiaRESUMO
We report on the development of a software program named iQuant2 which creates visual images from two-dimensional signal intensity data obtained by a laser ablation-ICP-mass spectrometry (LA-ICPMS) technique. Time-resolved signal intensity profiles can be converted to position resolved signal intensity data based on the rastering rate (µm s-1) of the laser ablation. Background signal intensities obtained without laser ablation (gas blank) are used as the background, and all of the blank-subtracted intensity data can be used for the imaging analysis. With this software, deformation of the created image can be corrected visually on a PC screen. The line profile analysis between the user-selected points can be observed using the iQuant2 software. To accomplish this, data points on the profile line were automatically calculated based on the interpolation between the analysis points. The resulting imaging data can be exported and stored as JPEG, BMP or PNG formats for further processing. Moreover, a semi-quantitative analysis can be made based on the coupling of the external correction of the RSF (relative sensitivity factor) using NIST SRM610 with normalization of the corrected signal intensity data being 100%. The calculated abundance data for major elements are in reasonable agreement with the values obtained by electron probe micro analyzer (EPMA). With the software developed in this study, both the rapid imaging and semi-quantitative determinations can be made.
RESUMO
The stimulation of bacterial activities that convert hexavalent uranium, U(VI), to tetravalent uranium, U(IV), appears to be feasible for cost-effective remediation of contaminated aquifers. However, U(VI) reduction typically results in the precipitation of U(IV) particles less than 5 nanometers in diameter, except for environmental conditions enriched with iron. Because these tiny particles are mobile and susceptible to oxidative dissolution after the termination of nutrient injection, in situ bioremediation remains to be impractical. Here we show that U(IV) nanoparticles of coffinite (U(SiO4)1-x(OH)4x) formed in fracture-filling calcium carbonate in a granitic aquifer. In situ U-Pb isotope dating demonstrates that U(IV) nanoparticles have been sequestered in the calcium carbonate for at least 1 million years. As the microbiologically induced precipitation of calcium carbonate in aquifer systems worldwide is extremely common, we anticipate simultaneous stimulation of microbial activities for precipitation reactions of calcium carbonate and U(IV) nanoparticles, which leads to long-term sequestration of uranium and other radionuclides in contaminated aquifers and deep geological repositories.
Assuntos
Bactérias/metabolismo , Água Subterrânea/microbiologia , Nanopartículas/metabolismo , Urânio/metabolismo , Datação RadiométricaRESUMO
We have developed a micro-electrothermal vaporization (µETV) device for the multiple collector-ICP-mass spectrometry (µETV-MC-ICPMS) to improve analytical precision in the (182)W/(183)W and (184)W/(183)W ratio measurements from nanogram quantities of W. The W solution was loaded onto the Re-filament, and the gradual evaporation of W was achieved by controlling the incident current onto the Re filament and D-Glucose. With the W evaporation under the Ar atmosphere, the measured W isotope ratios became erroneous mainly due to the contribution of signal spikes Ala-Arg-Gly-Phy-Tyr. In strike contrast, signal intensity profile became smooth when the He ambient/carrier gas was employed, and this resulted in better precision in the isotope ratio measurements. The measured UV-vis isotope ratio data obtained with present µETV technique were significantly deviated from the ratio data obtained with solution nebulization technique, mainly due to the contribution of the isotope fractionation effect through the evaporation process. Rigorous testing for the correction of the isotope fractionation processes pH-activity curve revealed that the Rayleigh fractionation law, rather than the conventional exponential law, provided the most reliable ratio data (1.851720±0.000018 for (182)W/(183)W and 2.141248±0.000028 for (184)W/(183)W ratios), which agreed well with the ratio data obtained through the conventional solution nebulization technique (1.851718±0.000039 for (182)W/(183)W and 2.141248±0.000022 for (184)W/(183)W). Moreover, mass dependency for the mass fractionation law suggested that W was evaporated as oxides (WO3), rather than the metallic form (W), from the Re filament, and therefore, information concerning the chemical form of the analytes could also be derived by the ETV technique developed in this study. The data presented here demonstrate clearly that the ETV sample introduction technique has a potential to become a sensitive tool for the precise isotope analysis for the MC-ICPMS technique.
RESUMO
Endochondral ossification at the growth plate is regulated by a number of factors and hormones. The cyclindependent kinase inhibitor p21 has been identified as a cell cycle regulator and its expression has been reported to be essential for endochondral ossification in vitro. However, to the best of our knowledge, the function of p21 in endochondral ossification has not been evaluated in vivo. Therefore, the aim of this study was to investigate the function of p21 in embryonic endochondral ossification in vivo. Wildtype (WT) and p21 knockout (KO) pregnant heterozygous mice were sacrificed on embryonic days E13.5, E15.5 and E18.5. Sagittal histological sections of the forearms of the embryos were collected and stained with Safranin O and 5bromo2'deoxyuridine (BrdU). Additionally, the expression levels of cyclin D1, type II collagen, type X collagen, Sox9, and p16 were examined using immunohistochemistry, and the expression levels of p27 were examined using immunofluorescence. Safranin O staining revealed no structural change between the cartilage tissues of the WT and p21KO mice at any time point. Type II collagen was expressed ubiquitously, while type X collagen was only expressed in the hypertrophic zone of the cartilage tissues. No differences in the levels of Sox9 expression were observed between the two groups at any time point. The levels of cyclin D1 expression and BrdU uptake were higher in the E13.5 cartilage tissue compared with those observed in the embryonic cartilage tissue at subsequent time points. Expression of p16 and p27 was ubiquitous throughout the tissue sections. These results indicate that p21 may not be essential for embryonic endochondral ossification in articular cartilage of mice and that other signaling networks may compensate for p21 deletion.
Assuntos
Cartilagem/embriologia , Cartilagem/metabolismo , Inibidor de Quinase Dependente de Ciclina p21/genética , Osteogênese/genética , Animais , Proliferação de Células , Condrócitos/citologia , Condrócitos/metabolismo , Inibidor p16 de Quinase Dependente de Ciclina/genética , Inibidor p16 de Quinase Dependente de Ciclina/metabolismo , Inibidor de Quinase Dependente de Ciclina p21/deficiência , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Inibidor de Quinase Dependente de Ciclina p27/genética , Inibidor de Quinase Dependente de Ciclina p27/metabolismo , Proteínas da Matriz Extracelular/genética , Proteínas da Matriz Extracelular/metabolismo , Expressão Gênica , Camundongos , Camundongos Knockout , Fatores de Transcrição SOX9/genética , Fatores de Transcrição SOX9/metabolismoRESUMO
The potential relationship between cell cycle checkpoint control and tissue regeneration has been indicated. Despite considerable research being focused on the relationship between p21 and myogenesis, p21 function in skeletal muscle regeneration remains unclear. To clarify this, muscle injury model was recreated by intramuscular injection of bupivacaine hydrochloride in the soleus of p21 knockout (KO) mice and wild type (WT) mice. The mice were sacrificed at 3, 14, and 28 days post-operation. The results of hematoxylin-eosin staining and immunofluorescence of muscle membrane indicated that muscle regeneration was delayed in p21 KO mice. Cyclin D1 mRNA expression and both Ki-67 and PCNA immunohistochemistry suggested that p21 deficiency increased cell cycle and muscle cell proliferation. F4/80 immunohistochemistry also suggested the increase of immune response in p21 KO mice. On the other hand, both the mRNA expression and western blot analysis of MyoD, myogenin, and Pax7 indicated that muscular differentiation was delayed in p21KO mice. Considering these results, we confirmed that muscle injury causes an increase in cell proliferation. However, muscle differentiation in p21 KO mice was inhibited due to the low expression of muscular synthesis genes, leading to a delay in the muscular regeneration. Thus, we conclude that p21 plays an important role in the in vivo healing process in muscular injury.
Assuntos
Inibidor de Quinase Dependente de Ciclina p21/deficiência , Músculo Esquelético/fisiologia , Regeneração/genética , Animais , Antígenos de Diferenciação/metabolismo , Membrana Celular/metabolismo , Ciclina D1/genética , Inibidor de Quinase Dependente de Ciclina p21/genética , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Antígeno Ki-67/metabolismo , Camundongos , Camundongos Knockout , Desenvolvimento Muscular/genética , Músculo Esquelético/anatomia & histologia , Músculo Esquelético/citologia , Músculo Esquelético/lesões , Antígeno Nuclear de Célula em Proliferação/metabolismo , RNA Mensageiro/genética , Cicatrização/genéticaRESUMO
Eicosapentaenoic acid (EPA) is an antioxidant and n-3 polyunsaturated fatty acid that reduces the production of inflammatory cytokines. We evaluated the role of EPA in chondrocyte apoptosis and degeneration. Normal human chondrocytes were treated with EPA and sodium nitroprusside (SNP). Expression of metalloproteinases (MMPs) was detected by real-time polymerase chain reaction (PCR) and that of apoptosis-related proteins was detected by western blotting. Chondrocyte apoptosis was detected by flow cytometry. C57BL/6J mice were used for the detection of MMP expression by immunohistochemistry and for investigation of chondrocyte apoptosis. EPA inhibited SNP-induced chondrocyte apoptosis, caspase 3 and poly(ADP-ribose) polymerase cleavage, phosphorylation of p38 MAPK and p53, and expression of MMP3 and MMP13. Intra-articular injection of EPA prevented the progression of osteoarthritis (OA) by inhibiting MMP13 expression and chondrocyte apoptosis. EPA treatment can control oxidative stress-induced OA progression, and thus may be a new approach for OA therapy.
Assuntos
Apoptose/efeitos dos fármacos , Condrócitos/efeitos dos fármacos , Ácido Eicosapentaenoico/farmacologia , Estresse Oxidativo , Animais , Caspase 3/metabolismo , Células Cultivadas , Ácido Eicosapentaenoico/uso terapêutico , Humanos , Metaloproteinase 13 da Matriz/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Nitroprussiato/farmacologia , Osteoartrite/tratamento farmacológico , Fosforilação , Proteína Supressora de Tumor p53/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismoRESUMO
Phosphatase and tensin homologue deleted on chromosome 10 (PTEN) was identified as an important tumor suppressor gene. PTEN functions as a negative regulator of phosphoinositol-3-kinase (PI3K)-Akt and MEK/ERK signaling. The PI3K-Akt pathway is critical for cell survival, differentiation, and matrix synthesis. Oxidative stress is considered a critical factor in the onset and progression of osteoarthritis (OA). Therefore, we investigated the function of PTEN in OA chondrocytes under oxidative stress. Chondrocytes were treated with insulin-like growth factor-1 (IGF-1) and/or tert-butyl hydroperoxide (tBHP), which causes oxidative stress. The expression levels of type2 collagen (Col2a1) and aggrecan were analyzed by real-time PCR, and phosphorylation of Akt and ERK1/2 was analyzed by Western blotting. Chondrocytes were treated with PTEN-specific small interfering RNA (siRNA), as well as IGF-1 and/or tBHP. PTEN and IGF-1 expressions in OA chondrocytes were increased. The downregulation of PTEN expression increased the expression levels of Col2a1 and aggrecan, and increased proteoglycan synthesis under oxidative stress. Oxidative stress decreased the phosphorylation of Akt and increased that of ERK1/2. The downregulation of PTEN expression increased Akt phosphorylation, but did not increase that of ERK 1/2. Our results suggest that PTEN regulates matrix synthesis via the PI3K-Akt pathway under oxidative stress.