RESUMO
A total of 75 papers published in 2017 were reviewed ranging from detailed descriptions of analytical methods, to fate and occurrence studies, to ecological effects and sampling techniques for a wide variety of emerging contaminants likely to occur in agricultural environments. New methods and studies on veterinary pharmaceuticals, steroids, antibiotic resistance genes, and engineered nanoparticles agricultural environments continue to expand our knowledge base on the occurrence and potential impacts of these compounds. This review is divided into the following sections: Introduction, Analytical Methods, Fate and Occurrence, Pharmaceutical Metabolites, Anthelmintics, Antibiotic Resistence Genes, and Engineered Nanomaterials.
Assuntos
Agricultura , Monitoramento Ambiental , Poluentes Ambientais/análise , Poluentes Ambientais/metabolismoRESUMO
A total of 79 papers published in 2016 were reviewed ranging from detailed descriptions of analytical methods, to fate and occurrence studies, to ecological effects and sampling techniques for a wide variety of emerging contaminants likely to occur in agricultural environments. New methods and studies on veterinary pharmaceuticals, steroids, antibiotic resistance genes, cyanotoxins, and potential effects of biochar use in agricultural environments continue to expand our knowledge base on the occurrence and potential impacts of these compounds. This review is divided into the following sections: Introduction, Analytical Methods, Steroid Hormones, Anthelmintics, Antibiotic Fate and Occurrence, Antibiotic Resistance Genes, Cyanotoxins, and Implications of Biochar in Agricultural Environments.
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Agricultura , Monitoramento Ambiental , Poluentes Ambientais/análise , EcologiaRESUMO
Large quantities of antibiotics are used in agricultural production, resulting in their release to agroecosystems through numerous pathways, including land application of contaminated manure, runoff from manure-fertilized fields, and wastewater irrigation of croplands. Antibiotics and their transformation products (TPs) exhibit a wide range of physico-chemical and biological properties and thus present substantive analytical challenges. Advances in the measurement of these compounds in various environmental compartments (plants, manure, soil, sediment, and water) have uncovered a previously unrealized landscape of antibiotic residues. These advanced multiresidue methods, designed to measure sub-ng g concentrations in complex mixtures, remain limited by the inherent intricacy of the sample matrices and the difficultly in eliminating interferences that affect antibiotic detection. While efficient extraction methods combined with high sensitivity analysis by liquid chromatography/mass spectrometry can provide accurate quantification of antibiotics and their TPs, measured concentrations do not necessarily reflect their bioavailable fractions and effects in the environment. Consequently, there is a need to complement chemical analysis with biological assays that can provide information on bioavailability, biological activity, and effects of mixtures. Enzyme-linked immunosorbent assays (ELISA), often used as screening tools for antibiotic residues, may be useful for detecting the presence of structurally related antibiotic mixtures but not their effects. Other tools, including bioreporter assays, hold promise in measuring bioavailable antibiotics and could provide insights on their biological activity. Improved assessment of the ecological and human health risks associated with antibiotics in agroecosystems requires continued advances in analytical accuracy and sensitivity through improvements in sample preparation, instrumentation, and screening technologies.
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Agricultura , Antibacterianos , Ecossistema , Saúde Ambiental , Esterco , Cromatografia Líquida , Poluentes Ambientais , Humanos , Solo , Poluentes do SoloRESUMO
We propose a simple causal model depicting relationships involved in dissemination of antibiotics and antibiotic resistance in agroecosystems and potential effects on human health, functioning of natural ecosystems, and agricultural productivity. Available evidence for each causal link is briefly summarized, and key knowledge gaps are highlighted. A lack of quantitative estimates of human exposure to environmental bacteria, in general, and antibiotic-resistant bacteria, specifically, is a significant data gap hindering the assessment of effects on human health. The contribution of horizontal gene transfer to resistance in the environment and conditions that might foster the horizontal transfer of antibiotic resistance genes into human pathogens also need further research. Existing research has focused heavily on human health effects, with relatively little known about the effects of antibiotics and antibiotic resistance on natural and agricultural ecosystems. The proposed causal model is used to elucidate gaps in knowledge that must be addressed by the research community and may provide a useful starting point for the design and analysis of future research efforts.
Assuntos
Antibacterianos , Resistência Microbiana a Medicamentos , Ecossistema , Agricultura , Bactérias , HumanosRESUMO
A total of 59 papers published in 2015 were reviewed ranging from detailed descriptions of analytical methods, to fate and occurrence studies, to ecological effects and sampling techniques for a wide variety of emerging contaminants likely to occur in agricultural environments. New methods and studies on veterinary pharmaceuticals, steroids, antibiotic resistance genes in agricultural environments continue to expand our knowledge base on the occurrence and potential impacts of these compounds. This review is divided into the following sections: Introduction, Analytical Methods, Steroid Hormones, Pharmaceutical Contaminants, Transformation Products, and "Antibiotic Resistance, Drugs, Bugs and Genes".
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Agricultura , Monitoramento Ambiental , Poluentes Ambientais , Ecologia , Preparações FarmacêuticasRESUMO
A total of 43 papers published in 2014 were reviewed ranging from detailed descriptions of analytical methods, to fate and occurrence studies, to measuring and predicting biological effects for a wide variety of emerging contaminants likely to occur in agricultural environments. New methods and studies on veterinary pharmaceuticals, natural and synthetics steroids, and antibiotic resistance genes in agricultural environments continue to expand our knowledge base on the occurrence and potential impacts of these compounds. This review is divided into the following sections: Introduction, Analytical Methods, Occurrence and Fate, Antibiotic Resistance Genes, and Risk Assessment.
RESUMO
There is growing concern about the prevalence and impact of contaminants of emerging concern (CECs). The environmental monitoring of CECs has, however, been limited in low- and middle-income countries due to the lack of advanced analytical instrumentation locally. In the present study we employed a nontargeted and suspect screening workflow via liquid chromatography coupled with high-resolution mass spectrometry (HRMS) to identify known and unknown pollutants in the Glen Valley wastewater treatment plant, Botswana, complemented by analysis of groundwater samples. The present study represents the first HRMS analysis of CECs in water samples obtained in Botswana. Suspect screening of 5942 compounds qualitatively identified 28 compounds, including 26 pharmaceuticals and two illicit drugs (2-ethylmethcathinone and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol). Nontargeted analysis tentatively identified the presence of 34 more compounds including (5ξ)-12,13-dihydroxypodocarpa-8,11,13-trien-7-one, 12-aminododecanoic acid, atenolol acid, brilliant blue, cyclo leucylprolyl, decanophenone, DL-carnitine, N,N'-dicyclohexylurea, N4-acetylsulfamethoxazole, NP-003672, and 24 polyethylene glycol polymers. The highest number of detections were in influent wastewater (26 CECs) followed by effluent wastewater (10 CECs) and, lastly, groundwater (4 CECs). Seventeen CECs detected in the influent water were not detected in the effluent waters, suggesting reduced emissions due to wastewater treatment. Two antiretroviral compounds (abacavir and tenofovir) were detected in the influent and effluent sources. This suggests that wastewater treatment plants are a major pathway of chemical pollution to the environment in Botswana and will help inform prioritization efforts for monitoring and remediation that is protective of these key ecosystems. Environ Toxicol Chem 2024;43:52-61. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
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Águas Residuárias , Poluentes Químicos da Água , Ecossistema , Botsuana , Poluentes Químicos da Água/análise , Monitoramento Ambiental , ÁguaRESUMO
Development of risk-assessment methodologies for polymers is an emerging regulatory priority to prevent negative environmental impacts; however, the diversity and complexity of polymers require adaptation of existing environmental risk-assessment approaches. The present review discusses the challenges and opportunities for the fate and exposure assessment of polymers in the context of regulatory environmental risk assessment of chemicals. The review discusses the applicability and adequacy for polymers of existing fate parameters used for nonpolymeric compounds and proposes additional parameters that could inform the fate of polymers. The significance of these parameters in various stages of an exposure-assessment framework is highlighted, with classification of polymers as solid or dissolved being key for identification of those parameters most relevant to environmental fate. Considerations to address the key limitations and knowledge gaps are then identified and discussed, specifically the complexity of polymer identification, with the need for characterization of the most significant parameters for polymer grouping and prioritization; the complexity of polymer degradation in the environment, with the need to incorporate the fate and hazards of degradation products into risk assessment; the requirement for development and standardization of analytical methods for characterization of polymer fate properties and degradation products; and the need to develop exposure modeling approaches for polymers. Environ Toxicol Chem 2022;41:515-540. © 2021 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
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Ecotoxicologia , Polímeros , Exposição Ambiental , Medição de RiscoRESUMO
Soil salinity is a worldwide problem and is damaging soil functions. Meanwhile, increasing amounts of anthropogenic antibiotics are discharged to agricultural soils. Little is known about how soil salinity (e.g., NaCl) could influence the bioavailability of antibiotics to bacteria. In this study, a tetracycline-responsive Escherichia coli bioreporter grew on the surfaces of agar microcosms at the same tetracycline concentration (200 µg/L), but various NaCl concentrations (0.5-19.2 g/L) with estimated osmotic potential of -0.18 to -1.80 MPa, and agar content (0.3%-5%) with estimated intrinsic permeability of 38 to 32,928 nm2. These agar microcosms mimicked very fine textured soils with a range of NaCl salinity. Increasing agar content lowered the intrinsic permeability hence decreasing tetracycline bioavailability to E. coli, due likely to the reduced mass transfer of tetracycline via water flow. Intriguingly, tetracycline bioavailability increased with increasing NaCl concentration which caused the increase in osmotic stress. This is contradictory to the notion that osmotic stress reduces bacterial chemical uptake. Further analysis of E. coli membrane integrity demonstrated that the enhanced tetracycline bioavailability to bacteria could result from the compromised cell membranes and enhanced membrane permeability at higher NaCl salinity. Overall, this study suggests that high soil salinity (NaCl) may enhance the selection pressure exerted by antibiotics on bacteria.
Assuntos
Escherichia coli , Poluentes do Solo , Ágar , Antibacterianos/química , Bactérias/metabolismo , Disponibilidade Biológica , Escherichia coli/metabolismo , Salinidade , Cloreto de Sódio/metabolismo , Cloreto de Sódio/farmacologia , Solo , Poluentes do Solo/metabolismo , Tetraciclina/metabolismo , Tetraciclina/farmacologiaRESUMO
The growing production and use of chemicals and the resultant increase in environmental exposure is of particular concern in developing countries where there is rapid industrialization and population growth but limited information on the occurrence of emerging contaminants. Advances in analytical techniques now allow for the monitoring of emerging contaminants at very low concentrations with the potential to cause harmful ecotoxicological effects. Therefore, we provide the first critical assessment of the current state of knowledge about chemical exposure in waters of the Southern African Developmental Community (SADC). We achieved this through a comprehensive literature review and the creation of a database of chemical monitoring data. Of the 59 articles reviewed, most (n = 36; 61.0%) were from South Africa, and the rest were from Botswana (n = 6; 10.2%), Zimbabwe (n = 6; 10.2%), Malawi (n = 3; 5.1%), Mozambique (n = 3; 5.1%), Zambia (n = 2; 3.4%), Angola (n = 1; 1.7%), Madagascar (n = 1; 1.7%), and Tanzania (n = 1; 1.7%). No publications were found from the remaining seven SADC countries. Emerging contaminants have only been studied in South Africa and Botswana. The antiretroviral drug ritonavir (64.52 µg/L) was detected at the highest average concentration, and ibuprofen (17 times) was detected most frequently. Despite being the primary water source in the region, groundwater was understudied (only 13 studies). High emerging contaminant concentrations in surface waters indicate the presence of secondary sources of pollution such as sewage leakage. We identify research gaps and propose actions to assess and reduce chemical pollution to enable the SADC to address the Sustainable Development Goals, particularly Goal 3.9, to reduce the deaths and illnesses from hazardous chemicals and contamination. Environ Toxicol Chem 2022;41:382-395. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
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Água Subterrânea , Poluentes Químicos da Água , Exposição Ambiental , Monitoramento Ambiental/métodos , Substâncias Perigosas , Águas Residuárias , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidadeRESUMO
Coral reefs are among the world's most productive and biologically diverse ecosystems. In recent decades, they have experienced an unparalleled decline resulting from various anthropogenically induced stressors. Ultraviolet (UV) filters found in personal care products, such as sunscreen, are chemical pollutants that are emerging as a growing toxic threat to reef organisms. In this study, a systematic literature review was conducted to (1) determine the current understanding of spatial distribution and the occurrence of UV filters exposed to the marine environment, (2) synthesize current ecotoxicological thresholds of relevant reef organisms under various UV-filter exposures, (3) identify research gaps related to both exposure and toxicity of UV filters in coral reef ecosystems. With gaps identified, a survey was developed and distributed to experts in the field representing academic, governmental, not-for-profit, and industry researchers in order to prioritize research gaps and inform future research efforts. The survey identified the need for better understanding of the impacts of co-stressors, long-term exposure, mixture, and degradation product exposure and realistic environmental conditions. Ultimately, this review will help guide priority research efforts to understand the risks of UV-filter exposure to coral reef ecosystems. Integr Environ Assess Manag 2021;17:967-981. © 2021 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
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Antozoários , Recifes de Corais , Animais , Ecossistema , Protetores Solares/toxicidadeRESUMO
Plant uptake is an important process in determining the transfer of pesticides through a food chain. Understanding how crops take up and translocate pesticides is critical in developing powerful models to predict pesticide accumulation in agricultural produce and potential human exposure. Herein, wheat was selected as a model plant species to investigate the uptake and distribution of eleven widely used pesticides in a hydroponic system as a function of time for 144 h. The time-dependent uptake kinetics of these pesticides were fitted with a first-order 1-compartment kinetic model. During 144 h, flusilazole and difenoconazole, with relative high log Kow (3.87 and 4.36, respectively), displayed higher root uptake rate constants (k). To clarify the role of root lipid content (flip) in plant accumulation of pesticides, we conducted a lipid normalization meta-analysis using data from this and previous studies, and found that the flip value was an important factor in predicting the root concentration factor (RCF) of pesticides. An improved correlation was observed between log RCF and log flipKow (R2 = 0.748, N = 26, P < 0.001), compared with the correlation between log RCF and log Kow (R2 = 0.686, N = 26, P < 0.001). Furthermore, the hydrophilic pesticides (e.g. log Kow < 2) were found to reach partition equilibrium faster than lipophilic pesticides (e.g. log Kow > 3) during the uptake process. The quasi-equilibrium factor (αpt) was inversely related to log Kow (R2 = 0.773, N = 11, P < 0.001) suggesting a hydrophobicity-regulated uptake equilibrium. Findings from this study could facilitate crop-uptake model optimization.
Assuntos
Praguicidas , Triticum , Transporte Biológico , Humanos , Cinética , Raízes de PlantasRESUMO
Polychlorinated dibenzo-p-dioxins and -furans (PCDD/PCDFs) are highly toxic organic pollutants in soils and sediments which persist over timescales that extend from decades to centuries. There is a growing need to develop effective technologies for remediating PCDD/Fs-contaminated soils and sediments to protect human and ecosystem health. The use of sorbent amendments to sequester PCDD/Fs has emerged as one promising technology. A synthesis method is described here to create a magnetic activated carbon composite (AC-Fe3O4) for dioxin removal and sampling that could be recovered from soils using magnetic separation. Six AC-Fe3O4 composites were evaluated (five granular ACs (GACs) and one fine-textured powder AC(PAC)) for their magnetization and ability to sequester dibenzo-p-dioxin (DD). Both GAC/PAC and GAC/PAC-Fe3O4 composites effectively removed DD from aqueous solution. The sorption affinity of DD for GAC-Fe3O4 was slightly reduced compared to GAC alone, which is attributed to the blocking of sorption sites. The magnetization of a GAC-Fe3O4 composite reached 5.38 emu/g based on SQUID results, allowing the adsorbent to be easily separated from aqueous solution using an external magnetic field. Similarly, a fine-textured PAC-Fe3O4 composite was synthesized with a magnetization of 9.3 emu/g.
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Dioxinas , Dibenzodioxinas Policloradas , Carvão Vegetal , Dibenzofuranos , Dibenzofuranos Policlorados , Ecossistema , HumanosRESUMO
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a toxic and persistent organic pollutant found in soils and sediments. It has been linked to several adverse health outcomes in humans and wildlife, including suppression of the immune system. TCDD is strongly sorbed to soils/sediments due to its extremely low water solubility. Presently, the bioavailability of soil/sediment-sorbed TCDD to mammals is not completely understood. Our previous studies demonstrated that TCDD adsorbed to representative inorganic geosorbents (i.e. porous silica and smectite clay) exhibited the same bioavailability to mice as TCDD dissolved in corn oil, whereas sequestration by activated carbons eliminated TCDD bioavailability. In this study, we evaluated the effects of amorphous natural organic matter (NOM), primarily in the form of aquatic humic and fulvic acids, on the mouse bioavailability of TCDD. An aqueous suspension of TCDD mixed with NOM was administered to mice via oral gavage. The relative bioavailability of TCDD was assessed by two sensitive aryl hydrocarbon receptor-mediated responses in mice: 1) hepatic induction of cyp1A1 mRNA; and 2) suppression of immunoglobulin M (IgM) antibody-forming cell (AFC) response which is an indicator of immunotoxicity. Hepatic induction of cyp1A1 mRNA and suppression of IgM AFC induced by TCDD were similar in the NOM-sorbed form and dissolved in corn oil, revealing no loss of bioavailability when associated with NOM. Hence, NOM-associated TCDD is as capable of suppressing humoral immunity in mice as TCDD dissolved in corn oil, indicating that NOM-sorbed TCDD is likely to fully retain its bioavailability to mammals and, by inference, humans.
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Dibenzodioxinas Policloradas , Animais , Disponibilidade Biológica , Carvão Vegetal , Citocromo P-450 CYP1A1/metabolismo , Mamíferos/metabolismo , Camundongos , Receptores de Hidrocarboneto Arílico , SoloRESUMO
A novel, low-cost, and portable paper strip biosensor was developed for the detection of tetracycline antibiotics. Escherichia coli/pMTLacZ containing the tetracycline-mediated regulatory gene used as recognition elements with ß-galactosidase as the reporter protein was designed and applied to cheap and portable Whatman filter paper as the carrier to prepare this paper strip biosensor. The detection process was optimized by using EDTA and polymyxin B as a sensitizer to improve the accuracy of detection for complicated matrices. The paper strip biosensor was suitable for tetracycline concentrations in the range of 75-10000 µg/L in water and 75-7500 µg/L in soil extracts. Detection limits of 5.23-17.1 µg/L for water and 5.21-35.3 µg/kg for the EDTA soil extracts were achieved at a response time of 90 min. The standard deviation (SD) of detected values by the biosensor paper strip compared to those determined by HPLC was between 13.4 and 59.6% for tetracycline and 2.01-33.5% for oxytetracycline in water and was between 6.22 and 72.8% for tetracycline and 5.90-43.4% for oxytetracycline in soil. This suggests that the paper strip biosensor was suitable for detecting both tetracycline and oxytetracycline in water, and could provide a suitable detection for extractable oxytetracycline in soils. Therefore, this biosensor provides a simple, economical, and portable piece of field kit for on-site monitoring of tetracyclines in a variety of environmental samples, such as pond water and agricultural soil that are susceptible to tetracycline pollution from feed additives and fertilization with livestock manure.
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Técnicas Biossensoriais , Oxitetraciclina , Antibacterianos , Tetraciclina , TetraciclinasRESUMO
Polychlorinated dibenzo-p-dioxins and dibenzofurans are a group of chemically-related pollutants categorically known as dioxins. Some of their chlorinated congeners are among the most hazardous pollutants that persist in the environment. This persistence is due in part to the limited number of bacteria capable of metabolizing these compounds, but also to their limited bioavailability in soil. We used Sphingomonas wittichii strain RW1 (RW1), one of the few strains able to grow on dioxin, to characterize its ability to respond to and degrade clay-bound dioxin. We found that RW1 grew on and completely degraded dibenzo-p-dioxin (DD) intercalated into the smectite clay saponite (SAP). To characterize the effects of DD sorption on RW1 gene expression, we compared transcriptomes of RW1 grown with either free crystalline DD or DD intercalated clay, i.e. sandwiched between the clay interlayers (DDSAP). Free crystalline DD appeared to cause greater expression of toxicity and stress related functions. Genes coding for heat shock proteins, chaperones, as well as genes involved in DNA repair, and efflux were up-regulated during growth on crystalline dioxin compared to growth on intercalated dioxin. In contrast, growth on intercalated dioxin up-regulated genes that might be important in recognition and uptake mechanisms, as well as surface interaction/attachment/biofilm formation such as extracellular solute-binding protein and LuxR. These differences in gene expression may reflect the underlying adaptive mechanisms by which RW1 cells sense and deploy pathways to access dioxin intercalated into clay. These data show that intercalated DD remains bioavailable to the degrading bacterium with implications for bioremediation alternatives.
Assuntos
Sphingomonas , Disponibilidade Biológica , Argila , Dioxinas , Expressão Gênica , Genoma BacterianoRESUMO
Pharmaceuticals in agricultural soils originating from irrigation with treated wastewater and land-applied biosolids can enter field crops. However, little is known about the role of pore water in plant uptake of pharmaceuticals from soils. In this study, the fate, uptake and distribution of fifteen commonly used pharmaceuticals in soil-water-radish systems were investigated to examine the relationship between the accumulation and their physicochemical processes in soils. The results indicate that the distribution of pharmaceuticals between soil and pore water, as well as their biodegradation, combined to govern the bioavailability of pharmaceuticals to plant uptake. Fourteen out of 15 pharmaceuticals could enter radish tissues in which the accumulation ranged from 2.1 to 14080â¯ng/g. Comparison of bioconcentration factors (BCFs) on the basis of pharmaceutical concentration in bulk soil vs. in pore water implies that pharmaceuticals present in soil pore water are the major bioavailable fractions to plant uptake. The pore water-based BCFs exhibited a positive linear relationship with log Dow for the pharmaceuticals with >90% as neutral species in soil pore water, while such relationship was not observed between bulk soil-based BCFs and log Dow mainly due to sorption by soil. Other than hydrophobicity, the dissociation of ionizable pharmaceuticals in the soil pore water and (or) root cells may lead to the "ion-trap" effects and thus influence the uptake and translocation process. The large molecular-size pharmaceuticals (e.g., tylosin) manifested a minimum uptake due plausibly to the limited permeability of cell membranes.
Assuntos
Poluentes do Solo , Solo , Biodegradação Ambiental , Plantas , ÁguaRESUMO
The dissemination of pharmaceuticals in agroecosystems originating from land application of animal manure/sewage sludge and irrigation with treated wastewater in agricultural production has raised concern about the accumulation of pharmaceuticals in food products. The pathways of pharmaceutical entries via plant roots, transport to upper fractions, and the factors influencing these processes have yet been systematically elucidated, thus impeding the development of effective measures to mitigate pharmaceutical contamination in food crops. In this study, lettuce uptake of thirteen commonly used pharmaceuticals was investigated using a hydroponic experimental setting. Pharmaceutical sorption by lettuce roots was measured in order to evaluate the influence on pharmaceutical transport from roots to shoots. Small-sized pharmaceuticals e.g., caffeine and carbamazepine with molecular weight (MW) <300â¯gâ¯mol-1 and a low affinity to lettuce roots (sorption coefficient Kpâ¯<â¯0.05â¯Lâ¯g-1) manifested substantial transport to shoots. Small-sized molecules lamotrigine and trimethoprim had a relatively strong affinity to lettuce roots (Kpâ¯>â¯12.0â¯Lâ¯g-1) and demonstrated a reduced transport to shoots. Large-sized pharmaceuticals (e.g. MW >400â¯gâ¯mol-1) including lincomycin, monensin sodium, and tylosin could be excluded from cell membranes, resulting in the predominant accumulation in lettuce roots. Large-sized oxytetracycline existed as zwitterionic species that could slowly enter lettuce roots; however, the relatively strong interaction with lettuce roots limits its transport to shoots. The mass balance analysis revealed that acetaminophen, ß-estradiol, carbadox, estrone and triclosan were readily metabolized in lettuce with >90% loss during 144-h exposure period. A scheme was proposed to describe pharmaceutical uptake and transport in plant, which could reasonably elucidate many literature-reported results. Molecular size, reactivity and ionic speciation of pharmaceuticals, as well as plant physiology, collectively determine their uptake, transport and accumulation in plants.
Assuntos
Lactuca/metabolismo , Poluentes Químicos da Água/metabolismo , Agricultura , Animais , Carbamazepina/metabolismo , Esterco , Raízes de Plantas/metabolismo , Esgotos , Triclosan/metabolismo , Águas Residuárias/químicaRESUMO
The use of activated carbon (AC) as an in situ sorbent amendment to sequester polychlorinated-dibenzo-p-dioxins and furans (PCDD/Fs) present in contaminated soils and sediments has recently gained attention as a novel remedial approach. This remedy could be implemented at much lower cost while minimizing habitat destruction as compared to traditional remediation technologies that rely on dredging/excavation and landfilling. Several prior studies have demonstrated the ability of AC amendments to reduce pore water concentrations and hence bioaccumulation of PCDD/Fs in invertebrate species. However, our recent study was the first to show that AC had the ability to sequester 2,3,7,8tetrachlorodibenzopdioxin (TCDD) in a form that eliminated bioavailability to a mammalian (mouse) model. Here we show that three commercially available ACs, representing a wide range of pore size distributions, were equally effective in eliminating the bioavailability of TCDD based upon two sensitive bioassays, hepatic induction of cyp1A1 mRNA and immunoglobulin M antibody-forming cell response. These results provide direct evidence that a wide range of structurally diverse commercially available ACs may be suitable for use as in situ sorbent amendments to provide a cost-effective remedy for PCDD/F contaminated soils and sediments. Potentially, adaption of this technology would minimize habitat destruction and be protective of ecosystem and human health.
Assuntos
Carvão Vegetal/química , Poluentes Ambientais/metabolismo , Dibenzodioxinas Policloradas/metabolismo , Animais , Disponibilidade Biológica , Citocromo P-450 CYP1A1/metabolismo , Poluentes Ambientais/análise , Poluentes Ambientais/toxicidade , Feminino , Camundongos , Dibenzodioxinas Policloradas/análise , Dibenzodioxinas Policloradas/toxicidadeRESUMO
Metabolism of pharmaceuticals in plants is important to evaluate their fate and accumulation in vegetables, and subsequently the risks to human health. However, limited knowledge is available to evaluate metabolism of pharmaceuticals in plants due to the lack of appropriate research approaches. In this study, radish was selected as a model plant to investigate metabolism of pharmaceuticals in intact plants (in vivo) growing in hydroponic solution and in plant tissue enzyme extracts (in vitro). For caffeine, six phase-I demethylation metabolites identified in the intact radish plant were also found in the plant enzyme extracts. After 7 days of in vivo exposure, the amount of the identified metabolites was about 5.4 times greater than the parent compound caffeine in radish roots. Furthermore, the metabolism potential of fifteen pharmaceuticals in radish was evaluated on the basis of mass balance. After 7 days of hydroponic exposure, oxytetracycline, trimethoprim, carbamazepine, lincomycin, monensin and tylosin manifested relatively less extent of metabolism with the mass recoveries ranging from 52.3 to 78.2%. In contrast, 17 ß-estradiol, sulfamethoxazole, sulfadiazine, estrone, triclosan, acetaminophen, caffeine, carbadox and lamotrigine underwent extensive metabolism with only 3.0 to 32.1% of the parent compound recovered. In the in vitro system, 17 ß-estradiol, estrone, triclosan, oxytetracycline, acetaminophen, sulfadiazine and sulfamethoxazole were readily metabolized in radish root enzyme extracts with 1.8 to 34.0% remaining after 96-h exposure. While in the leaf enzyme extracts, only triclosan was rapidly metabolized with 49.2% remaining, and others pharmaceuticals were ≥60%, indicating that the varying extents of metabolism occurred in different plant parts. This study highlights the importance of pharmaceutical metabolism in plants, and suggests that plant tissue enzyme extracts could serve as an alternative tool to assess pharmaceutical metabolism in plants.