Temperature and immigration effects on quorum sensing in the biofilms of anaerobic membrane bioreactors.

Quorum sensing (QS), a microbial communication mechanism modulated by acyl homoserine lactone (AHL) molecules impacts biofilm formation in bioreactors. This study investigated the effects of temperature and immigration on AHL levels and biofouling in anaerobic membrane bioreactors. The hypothesis was that the immigrant microbial community would increase the AHL-mediated QS, thus stimulating biofouling and that low temperatures would exacerbate this. We observed that presence of immigrants, especially when exposed to low temperatures indeed increased AHL concentrations and fouling in the biofilms on the membranes. At low temperature, the concentrations of the main AHLs observed, N-dodecanoyl-L-homoserine lactone and N-decanoyl-L-homoserine lactone, were significantly higher in the biofilms than in the sludge and correlated significantly with the abundance of immigrant bacteria. Apparently low temperature, immigration and denser community structure in the biofilm stressed the communities, triggering AHL production and excretion. These insights into the social behaviour of reactor communities responding to low temperature and influx of immigrants have implications for biofouling control in bioreactors.

Mitigation of membrane biofouling in membrane bioreactor treating sewage by novel quorum quenching strain of Acinetobacter originating from a full-scale membrane bioreactor.

A novel quorum quenching (QQ) strain, Acinetobacter guillouiae ST01, was isolated from a full-scale membrane bioreactor (MBR) and characterized for its QQ activities. Batch reactor studies at lab-scale showed that A. guillouiae ST01 exhibited higher QQ activity against acyl homoserine lactones (AHLs) with an oxo group compared to those without an oxo group. The organism was then inoculated (10%) in an MBR (Q-MBR) treating sewage over 48 days and was found to reduce quorum sensing (QS) activity by reducing AHL concentrations in the sludge and the biofilm of the Q-MBR. The concentration of polysaccharides was reduced up to 30% in both the biofilm and sludge relative to the control, whereas protein concentrations were reduced by 40% and 47% in the sludge and biofilm, respectively. The Q-MBR fouling rates were halved. These results indicate that A. guillouiae ST01 is a promising strain for biofouling reduction in MBR treating real wastewater.

Co-digestion of microalgae with potato processing waste and glycerol: effect of glycerol addition on methane production and the microbial community.

The production of methane-rich biogas from the anaerobic digestion (AD) of microalgae is limited by an unfavorable biomass carbon-to-nitrogen (C/N) ratio; however, this may be ameliorated using a co-digestion strategy with carbon-rich feedstocks. For reliable plant operation, and to improve the economics of the process, secure co-feedstock supply (ideally as a waste-stream) is important. To this end, this study investigated the feasibility of co-digesting microalgae (Chlorella vulgaris) with potato processing waste (potato discarded parts, PPWdp; potato peel, PPWp) and glycerol, while monitoring the response of the methanogenic community. In this semi-continuous study, glycerol (1 and 2% v/v) added to mixtures of C. vulgaris : PPWdp enhanced the specific methane yields the most, by 53-128%, whilst co-digestion with mixtures of C. vulgaris : PPWp enhanced the methane yields by 62-74%. The microbial communities diverged markedly over operational time, and to a lesser extent in response to glycerol addition. The acetoclast Methanosaeta was abundant in all treatments but was replaced by Methanosarcina in the potato peel with glycerol treatment due to volatile fatty acid (VFA) accumulation. Our findings demonstrate that the performance of microalgae co-digestion is substantially improved by the addition of glycerol as an additional co-feedstock. This should improve the economic case for anaerobically digesting microalgae as part of wastewater treatment processes and/or the terminal step of a microalgae biorefinery.

Performance of an up-flow anaerobic stage reactor (UASR) in the treatment of pharmaceutical wastewater containing macrolide antibiotics.

The performance of an up-flow anaerobic stage reactor (UASR) treating pharmaceutical wastewater containing macrolide antibiotics was investigated. Specifically, it was determined whether a UASR could be used as pre-treatment system at an existing pharmaceutical production plant to reduce the antibiotics in the trade effluent. Accordingly, a UASR was developed with an active reactor volume of 11 L being divided into four 2.75 L stages. Each stage of the reactor was an up-flow sludge blanket reactor and had a 3-phase separator baffle to retain biomass. The reactor was fed with real pharmaceutical wastewater containing Tylosin and Avilamycin antibiotics and operated with step-wise increases in the reactor organic loading rate (OLR) from 0.43 to 3.73 kg chemical oxygen demand (COD) m(-3)d(-1), and then reduced to 1.86, over 279 days. The process performance of the reactor was characterised in terms of its COD removal, Tylosin reduction, pH, VFA production, methane yield and sludge washout. At a total hydraulic retention time (HRT) of 4 d and OLR of 1.86 kg COD m(-3)d(-1), COD reduction was 70-75%, suggesting the biomass had acclimated to the antibiotics. Furthermore, an average of 95% Tylosin reduction was achieved in the UASR, indicating that this antibiotic could be degraded efficiently in the anaerobic reactor system. In addition, the influence of elevated Tylosin concentrations on the UASR process performance was studied using additions of Tylosin phosphate concentrate. Results showed similar efficiency for COD removal when Tylosin was present at concentrations ranging from 0 to 400 mgL(-1) (mean removal over this range was 93%), however, at Tylosin concentrations of 600 and 800 mgL(-1) there was a slight decline in treatment efficiency at 85% and 75% removal, respectively.

The steady state anaerobic digestion of Laminaria hyperborea--effect of hydraulic residence on biogas production and bacterial community composition.

Methane production by anaerobic digestion (AD) of macroalgae (seaweed) is a promising algal bioenergy option. Work presented here is primarily based on the AD of Laminaria hyperborea using batch and continuously stirred tank reactors. Extrapolation of data from batch studies to long term continuous reactors was unreliable. A conservative organic loading rate (OLR) of 1 g L(-1) d(-1) was used due to difficulties experienced in achieving steady state performance at an OLR of 1.5 g L(-1) d(-1). Biogas composition and methane yields (60-70%) were near to values expected from terrestrial feedstocks. Biomass washout, as imposed by the dilution rate (i.e., hydraulic residence), had considerable bearing on the biogas generation profile, particularly at >3 hydraulic residences. Inhibition of methanogen growth was linked to nutrient deficiency and potentially antimicrobial compounds associated with the feedstock. Anaerobic digestion of L. hyperborea proved feasible over extended operational periods.

Impact of the herbicide (RS)-MCPP on an anaerobic membrane bioreactor performance under different COD/nitrate ratios.

The degradation of (RS)-MCPP was investigated in an anaerobic membrane bioreactor (AnMBR) using nitrate as an available electron acceptor under different COD/NO(3)(-)-N ratios. Results showed high soluble COD removal efficiency (80-93%) when the reactor was operated at high COD/NO(3)(-)-N ratios. However, the COD removal started to decline (average 15%) at high nitrate concentrations coinciding with a drop in nitrate removal efficiency to 37%, suggesting that the denitrification activity dropped and affected the AnMBR performance when nitrate was the predominant electron acceptor. Additionally, the removal efficiency of (RS)-MCPP increased from 2% to 47% with reducing COD/NO(3)(-)-N ratios, whilst the (RS)-MCPP specific utilisation rate (SUR) was inversely proportional to the COD/NO(3)(-)-N ratio, suggesting that a lower COD/NO(3)(-)-N ratios had a positive influence on the (RS)-MCPP SUR. Although nitrate had a major impact on methane production rates, the methane composition was stable (approximately 80%) for COD/NO(3)(-)-N ratios of 23 or more.

Influence of step increases in hydraulic retention time on (RS)-MCPP degradation using an anaerobic membrane bioreactor.

The effects of different hydraulic retention time (HRT) on (RS)-MCPP utilisation was investigated by decreasing the feed flow rate in an anaerobic membrane bioreactor (AnMBR). Results showed an average COD removal efficiency of 91.4%, 96.9% and 94.4% when the reactor was operated at HRT 3, 7 and 17 d, respectively. However, when the HRT was reduced to 1d, the COD removal efficiency declined to just only 60%, confirming the AnMBR is stable to a large transient hydraulic shock loads. The (RS)-MCPP removal efficiency fluctuated from 6% to 39% at HRT 3 d, however when it was increased to 7 and 17 d, the removal efficiency increased to an average of 60% and 74.5%. In addition, (RS)-MCPP specific utilisation rates (SUR) were dependent on the HRT and gradually improved from 18 to 43 µg mg VSS(-1) d(-1) as flow rate increased.

Fate of estrogens during the biological treatment of synthetic wastewater in a nitrite-accumulating sequencing batch reactor.

The fate of estrone (El), 17beta-estradiol (E2), and 17alpha-ethynylestradiol (EE2) was investigated in two nitrite-accumulating sequencing batch reactors operating under strictly aerobic (SBR1) conditions at different sludge ages (SRT, 1.7 to 17.1 d) and anoxic/anaerobic/aerobic (SBR2) conditions with different phases and durations of redox conditions, using a modified GC-MS analytical method for estrogen detection to ng/L concentrations. In SBR1, > or =98% of E2 was removed (specific E2 removal rate ranged from 0.375 (at SRT 17.1 d) to 2.625 (at SRT 1.7 d) microg E2 x g MLVSS(-1) x d(-1)) regardless of SRT or DO (<1.0 to >5.0 mg/L). Removal of E1 and, to a greater extent, EE2 was adversely affected when this reactor was operated at SRT shorterthan 5.7 d. However, whereas E1 was removed efficiently as long as SRT was long enough for AOB to bring about nitritation, EE2 removal efficiency was significantly lower when SBR1 was operated at SRT longer than 7.5 d. This reduced removal of EE2 may have been caused by the inhibition and toxicity of nitrite, both to the ammonium monooxygenase (AMO) and to the microbial population generally. In SBR2, less removal of E2 was found at the lower MLVSS concentrations, and E1 was not removed by sludge with poor settling qualities. The removal of EE2 observed in SBR2 was considered to be mainly a result of sorption. However, the binding of estrogens to the sludge in this reactor was apparently not as strong as the binding observed in the sludge of the strictly aerobic SBR1, since desorption was observed during the aeration phase in SBR2.