MATTI - a multi-criteria decision analysis framework for assessing wastewater treatment technologies.

The variety of available technologies and the low investment power in sanitation services, especially in regions with low and emerging economies, makes the selection of an optimal wastewater treatment system design an even more complex task for decision-makers. Thus, this study aimed to develop a multi-criteria analysis-based tool to support decision-making on the optimal wastewater treatment technology for the needs and priorities of each region, the Most Appropriate Treatment Technology Index (MATTI). The methodology to apply the MATTI comprises five steps: select suitable technologies; determine the effluent quality parameters; define the most relevant variables for design; normalize and define the weighting criteria; and calculate the level of compliance (0-1, on an increasing scale of suitability). To validate the tool, two different scenarios and seven variables for the Brazilian context were simulated. Different sets of technologies scored above 0.75, and were classified as highly recommended, according to the weight criteria attributed to each scenario. MATTI not only contributed to improving the decision-making process, but also with a more global vision of the parameters to be considered in the selection of technologies to meet the needs and priorities.

Phycoremediation potential of microalgae species for ethidium bromide removal from aqueous media.

Ethidium Bromide (EtBr) is an organic compound used in molecular biology investigations. EtBr ability of intercalating in the DNA molecule makes it a toxic substance. The objective was to evaluate the phycoremediation potentials of Chlorella vulgaris, Desmodesmus subspicatus and Raphidocelis subcapitata tested separately and in a mixture (Mix) for EtBr removal from the aqueous medium. Experiments were conducted using an initial algae biomass of 106 cell/mL, exposed to 500 µg/L of EtBr. The removal efficiency (µg EtBr L-1) after 3 h in each treatment were: Mix (72.8 µg.L-1) >D. subspicatus (48.4 µg.L-1) >R. subcapitata (24.6 µg.L-1) >C. vulgaris (19.9 µg.L-1). However, when EtBr mass reduction per microalgae density is considered (ng.algae-1), the efficiency ranking changes to: D. subspicatus (1.9 × 10-5 ng.algae-1) >C. vulgaris (1.4 × 10-5 ng.algae-1) >Mix (9.8 × 10-6 ng.algae-1) >R. subcapitata (2.8 × 10-6 ng.algae-1). The results suggest that initial algal population density is a determinant factor for efficient EtBr removal by microalgae species in short term treatments. In order to obtain 100% of EtBr removal, it should be necessary 1010, 1010 and 1011 algae.mL-1 of C. vulgaris, D. subspicatus and R. subcapitata, respectively. The results strongly suggest phycoremediation can be explored as an alternative method for EtBr removal.

Effects of single and mixed estrogens on single and combined cultures of D. subspicatus and P. subcapitata.

This paper investigates the effect of estrone (E1), 17ß-estradiol (E2) and 17α-ethinylestradiol (EE2) individually and mixed at equal proportions (1:1:1) on Desmodesmus subspicatus and Pseudokirchneriella subcapitata in single and combined cultures (S+) at different exposure times based on algal growth (in vivo chlorophyll fluorescence and cell counting) and coenobium formation. EE2 and E2 were more toxic to individual and combined (S+) cultures than was E1. The frequency of coenobium formation by D. subspicatus increased significantly for all estrogens and all concentrations. After 96 h, D. subspicatus prevailed in S+. The results of the exposure to E+ suggested a less-than-additive effect on D. subspicatus and S+ and additive effect on P. subcapitata. Toxic effects occurred for both species exposed to E+ with individual estrogen concentrations below the NOEC of each species. Assays must include changes in response due to the exposure of more than one species to more than one estrogen.

Extração de biomassa aderida ao meio-suporte de um reator de leito móvel com biofilme: agitação mecânica e ultrassom / Extraction of biomass attached to a support media in a moving bed biofilm reactor: mechanical agitation and ultrasound

RESUMO A quantificação de biomassa fixa em reatores de leito móvel com biofilme (MBBRs) é essencial para avaliação e controle do desempenho deles. Entretanto, observa-se que tal quantificação é realizada empregando-se diversas técnicas, o que dificulta comparações entre resultados. No presente trabalho, foram testadas duas das principais técnicas (agitação mecânica e ultrassom) utilizadas para extração de biomassa aderida aos meios-suporte (mídias) de um MBBR sem utilização de agentes químicos. Os desenhos experimentais foram realizados utilizando-se planejamento fatorial completo, e, para tal, foram selecionadas duas variáveis independentes: tempo (T) de exposição de 15 a 60 min; e volume de água destilada utilizado para extração (V) de 100 a 400 mL. O ultrassom mostrou-se superior à agitação mecânica quanto à eficiência no percentual extraído de sólidos suspensos totais (%SST), e embora nenhuma das variáveis (T ou V) tenha contribuído significativamente nas faixas testadas, com base na superfície de respostas, foi conduzido um novo experimento, ampliando a faixa de T de 5 a 90 min. A variável T contribuiu significativamente para o %SST, e o melhor resultado foi obtido com T = 90 min. Recomenda-se o uso de ultrassom, volume de mais fácil manuseio (250 mL) e T = 90 min. Ensaios adicionais são recomendados, tendo em vista a padronização do método.

ABSTRACT Quantification of attached biomass in Moving Bed Biofilm Reactors (MBBR) is essential for performance assessment and process control. However, it is observed that such quantification is performed using the most diverse techniques, which hampers comparisons between results. In this investigation, two of the main techniques used (mechanical agitation and ultrasound) were compared for removal of the biomass attached to the support media of an MBBR reactor without the use of chemical agents. The experiments were planned following full factorial designs using the two independent variables: Exposure time (T) from 15 to 60 min; and Volume of distillate water used for extraction (V) from 100 to 400 mL. The results revealed that ultrasound is superior to mechanical agitation regarding detachment of the total suspended solids (%TSS) and though neither T or V contributed significantly within the interval tested, based on the response surface, a new experiment was performed using an increased T range from 5 to 90 min. Then, exposure time contributed significantly to the %TSS detachment, with the best result at 90 min. The use of ultrasound is therefore recommended, with volume that is easier to handle (250 mL) and time equal to 90 min. Additional tests are recommended for method standardization.