Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitis.

This study aimed to evaluate the effect of Bifidobacterium animalis subsp. lactis (B. lactis) HN019 in drinking water on the development of apical periodontitis (AP) in rats. In total 60 animals were divided into a control group (sound teeth); Group I - regular water without AP; Group II - probiotic water without AP; Group III - regular water with AP; Group IV - probiotic water with AP. AP was induced after 3 days in the control groups and after 7, 21, and 42 days in groups III and IV. The animals were euthanized, and the mandibles were subjected to histotechnical processing. Samples were stained with hematoxylin & eosin (H&E) to identify root canal features, apical and periapical regions. Additionally, histoenzymology was performed to detect osteoclasts, immunohistochemistry was used to identify osteoclastogenesis markers, and the Brown & Brenn technique was applied for microbiological analysis. The data were analyzed using GraphPad Prism 8.0.1 with a significance level of 5%. Although no statistical differences were observed, the groups administered with probiotics showed better conditions in terms of histological aspects seen microscopically. Furthermore, there were no differences in the number of osteoclasts (p > 0.05). The RANKL marker was not found in the probiotic group at 42 days, unlike in group III.

Effects of sodium lauryl sulphate (SLS), present in dentifrice, on volatile sulphur compound (VSC) formation in morning bad breath.

The purpose of the present investigation was to evaluate the effects of sodium lauryl sulphate (SLS), present in a commercial dentifrice, on the formation of volatile sulphur compounds (VSC) and tongue coating in a panel of periodontally healthy subjects. A two-step blinded, crossover, randomized study was carried out in 25 dental students with healthy periodontium; these were divided into two experimental groups: SLS (dentifrice with SLS) and WSLS (dentifrice without SLS). The volunteers received the designated dentifrice and a new toothbrush for a 3x/day brushing regimen for 2 periods of 30 days. A seven-day washout interval was used between the periods. The assessed parameters were: plaque index (PI), gingival index (GI), organoleptic breath (ORG), VSC levels by portable sulphide monitor before (H1) and after (H2) cleaning of the tongue, tongue coating wet weight (TC) and benzoyl-DL-arginine-naphthylamide (BANA) test from tongue coating samples. The intra-group analysis showed a decrease in the median of organoleptic scores from 3 to 2 after 30 days for the SLS group (p < 0.05). The inter-group analysis showed lower values in ORG and H1 for the SLS group (p < 0.05). There was no difference between the amount of TC in SLS and WSLS groups. In the BANA test, the presence of SLS did not affect the BANA number of +/- results (p > 0.05). These findings suggest that sodium lauryl sulphate (SLS), present in dentifrice, appears to prevent VSC formation in morning bad breath regardless of the amount of tongue coating in periodontally healthy subjects.

Effect of systemic administration of Bifidobacterium animalis subsp. lactis HN019 on apical periodontitis

Abstract This study aimed to evaluate the effect of Bifidobacterium animalis subsp. lactis (B. lactis) HN019 in drinking water on the development of apical periodontitis (AP) in rats. In total 60 animals were divided into a control group (sound teeth); Group I - regular water without AP; Group II - probiotic water without AP; Group III - regular water with AP; Group IV - probiotic water with AP. AP was induced after 3 days in the control groups and after 7, 21, and 42 days in groups III and IV. The animals were euthanized, and the mandibles were subjected to histotechnical processing. Samples were stained with hematoxylin & eosin (H&E) to identify root canal features, apical and periapical regions. Additionally, histoenzymology was performed to detect osteoclasts, immunohistochemistry was used to identify osteoclastogenesis markers, and the Brown & Brenn technique was applied for microbiological analysis. The data were analyzed using GraphPad Prism 8.0.1 with a significance level of 5%. Although no statistical differences were observed, the groups administered with probiotics showed better conditions in terms of histological aspects seen microscopically. Furthermore, there were no differences in the number of osteoclasts (p > 0.05). The RANKL marker was not found in the probiotic group at 42 days, unlike in group III.

Use of aspartate aminotransferase in diagnosing periodontal disease: a comparative study of clinical and microbiological parameters.

The objective of this study was to assess the association between the levels of enzyme aspartate aminotransferase (AST) in gingival crevicular fluid (GCF) with the BANA hydrolysis microbiological test (Perioscan) and clinical periodontal diagnostic measurements, such as bleeding on probing, plaque index, gingival index, probing depth, and attachment level in patients with chronic periodontitis using an enzymatic test (PocketWatch). One hundred and forty-seven sites were evaluated in 22 patients with a probing depth of > or = 5 mm at selected sites. AST and BANA enzymatic tests were carried out, and clinical parameters recorded. Pearson's chi-square and Fisher's exact tests were used for statistical analysis. There was no statistical correlation between AST levels and any of the analyzed parameters. The lack of any association between the factors studied does not indicate, however, that the latter cannot be used in diagnosing the actual periodontal condition of patients and/or sites. However, more research should be carried out to evaluate the true relationship between AST and periodontal disease.

Flavoring agents present in a dentifrice can modify volatile sulphur compounds (VSCs) formation in morning bad breath.

This study aimed to evaluate the effects of a flavor-containing dentifrice on the formation of volatile sulphur compounds (VSCs) in morning bad breath. A two-step, blinded, crossover, randomized study was carried out in 50 dental students with a healthy periodontium divided into two experimental groups: flavor-containing dentifrice (test) and non-flavor-containing dentifrice (control). The volunteers received the designated dentifrice and a new toothbrush for a 3 X/day brushing regimen for 2 periods of 30 days. A seven-day washout interval was used between the periods. The assessed parameters were: plaque index (PI), gingival index (GI), organoleptic breath scores (ORG), VSC levels (as measured by a portable sulphide monitor) before (H1) and after (H2) cleaning of the tongue, tongue coating (TC) wet weight and BANA test from TC samples. The intra-group analysis showed a decrease in ORG, from 3 to 2, after 30 days for the test group (p < 0.05). The inter-group analysis showed lower values in ORG, H1 and H2 for the test group (p < 0.05). There was no difference between the amount of TC between groups and the presence of flavor also did not interfere in the BANA results between groups (p > 0.05). These findings suggest that a flavor-containing dentifrice seems to prevent VSCs formation in morning bad breath regardless of the amount of TC in periodontally healthy subjects.

Flavoring agents present in a dentifrice can modify volatile sulphur compounds (VSCs) formation in morning bad breath

This study aimed to evaluate the effects of a flavor-containing dentifrice on the formation of volatile sulphur compounds (VSCs) in morning bad breath. A two-step, blinded, crossover, randomized study was carried out in 50 dental students with a healthy periodontium divided into two experimental groups: flavor-containing dentifrice (test) and non-flavor-containing dentifrice (control). The volunteers received the designated dentifrice and a new toothbrush for a 3 X/day brushing regimen for 2 periods of 30 days. A seven-day washout interval was used between the periods. The assessed parameters were: plaque index (PI), gingival index (GI), organoleptic breath scores (ORG), VSC levels (as measured by a portable sulphide monitor) before (H1) and after (H2) cleaning of the tongue, tongue coating (TC) wet weight and BANA test from TC samples. The intra-group analysis showed a decrease in ORG, from 3 to 2, after 30 days for the test group (p < 0.05). The inter-group analysis showed lower values in ORG, H1 and H2 for the test group (p < 0.05). There was no difference between the amount of TC between groups and the presence of flavor also did not interfere in the BANA results between groups (p > 0.05). These findings suggest that a flavor-containing dentifrice seems to prevent VSCs formation in morning bad breath regardless of the amount of TC in periodontally healthy subjects.

Correlação entre as medidas de compostos sulfurados voláteis (csv) no hálito matinal e o teste bana de saburra lingual / Correlation between the measurements of volatile sulphur compounds in the morning breath and BANA test of tongue coating

O objetivo deste estudo foi avaliar a correlação existente entre o teste BANA e os testes utilizados para diagnosticar a presença de compostos sulfurosos voláteis (CSV), do hálito matinal, numa amostra de 50 indivíduos periodontalmente saudáveis, após 30 dias de acúmulo de saburra lingual. Os voluntários receberam um mesmo dentifrício e uma escova dental nova para um regime de três escovações diárias, sem a limpeza da língua, durante 30 dias. Os parâmetros clínicos avaliados foram: mensuração organoléptica (ORG), mensuração dos níveis de CSV com a utilização de um monitor portátil, realizada antes (H1) da limpeza da língua e outra logo após (H2), remoção, coleta e pesagem da saburra lingual (TC), índice de placa (IP) e índice gengival (IG). A saburra lingual coletada foi utilizada para a realização do teste BANA. Todas as mensurações foram realizadas no início do estudo e 30 dias após. A análise estatística dos dados não demonstrou diferença para nenhum parâmetro avaliado entre o início e o final do estudo. Quando as correlações foram avaliadas, o teste BANA não apresentou correlação com nenhum parâmetro (IP, IG, H1, H2 e TC). Dentro dos limites deste estudo, pode-se concluir que em uma amostra de indivíduos periodontalmente saudáveis não houve correlação entre os parâmetros de hálito mensurados e o teste BANA em amostras de saburra lingual.

Pacientes especiais: avaliaçäo de três anos de perda óssea em radiografias panorâmicas / Special patients: a three years bone loss radiographic study

Para avaliar a perda óssea em pacientes especiais, foram selecionados 34 indivíduos (17 a 37 anos), divididos em dois grupos: portadores de Síndrome de Down (SD) e Retardo no Desenvolvimento Neuropsicomotor (RDNPM), os quais foram submetidos a exames radiográficos panorâmicos, inicial e após três anos. Nas radiografias, foram traçadas duas linhas nos sítios mesiais dos dentes 16, 26, 36 e 46, registrando Junçäo Cemento Esmalte (JCE) e Nível Ósseo Aleveolar (NOA). As distâncias JCE-NOA foram aferidas pelo programa Sigma Scan Pró. Decorridos três anos, a distância da JCE-NOA aumentou nos dois grupos e, comparativamente, foi maior no grupo Síndrome de Down

Cimentos ionoméricos empregados no tratamento restaurador atraumático (T. R. A.) / Glass ionomer cements employed in (A. R. T.)

O objetivo deste trabalho foi avaliar a atividade antimicrobiana de cimentos ionoméricos comumente empregados no Tratamento Restaurador Atraumático - Ketac-fil, Ketac Molar, Vidrion R, Fuji IX. Para tal, utilizou-se o teste de difusão em agar determinando o halo de inibição do crescimento bacteriano sobre Streptococcus luteus. Foram confeccionados espécimes sob condições assépticas, seguindo as instruções dos fabricantes. A seguir, os espécimes foram aplicados sobre os meios de cultura (MHa e BHIa) com pH ácido, contendo suspensão das cepas. Decorridos 2 horas de pré-incubação à temperatura ambiente, as placas foram incubadas a 37°C por 48 horas. Em seguida, verificou-se a presença ou ausência de zonas de inibição bacteriana em torno dos cimentos ionoméricos. Após análise das placas verificou-se que materiais testados apresentaram atividade antimicrobiana sobre todos os microrganismo indicadores. Concluindo-se que os ionômeros foram capazes de apresentar potencial antimicrobiano, podendo ser empregados na técnica TRA