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1.
Mater Sci Eng C Mater Biol Appl ; 110: 110673, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32204101

RESUMO

The metal corrosion inhibition efficiency of a novel synthesized cationic gemini surfactant (SCGS), namely, 4,4'-(((1E,5E)-pentane-1,5-diylidene)bis(azanylylidene))bis (1-dodecylpyridin-1-ium) bromide, was studied in acidic medium by three techniques. The achieved results displayed the inhibition efficiency of the metal corrosion that was elevated by increasing both the SCGS's concentration and the applied temperature values. Furthermore, it was noticed that the charge transfer resistance value was elevated; however, the constant phase element was decreased with increasing the SCGS concentrations. The SCGS regards an excellent and mixed-type corrosion inhibitor. The adsorption of SCGS has agreed the Langmuir's adsorption isotherm and was related to physisorption and chemisorption.


Assuntos
Ácidos/química , Compostos de Piridínio/química , Adsorção , Simulação por Computador , Corrosão , Espectroscopia Dielétrica , Espectroscopia de Prótons por Ressonância Magnética , Compostos de Piridínio/síntese química , Soluções , Espectroscopia de Infravermelho com Transformada de Fourier , Tensão Superficial , Tensoativos/síntese química , Tensoativos/química , Temperatura
2.
Biotech Histochem ; 91(8): 532-539, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27849398

RESUMO

Endothelial progenitor cells (EPC) participate in revascularization and angiogenesis. EPC can be cultured in vitro from mononuclear cells of peripheral blood, umbilical cord blood or bone marrow; they also can be transdifferentiated from mesenchymal stem cells (MSC). We isolated EPCs from Wharton's jelly (WJ) using two methods. The first method was by obtaining MSC from WJ and characterizing them by flow cytometry and their adipogenic and osteogenic differentiation, then applying endothelial growth differentiating media. The second method was by direct culture of cells derived from WJ into endothelial differentiating media. EPCs were characterized by morphology, Dil-LDL uptake/UEA-1 immunostaining and testing the expression of endothelial markers by flow cytometry and RT-PCR. We found that MSC derived from WJ differentiated into endothelial-like cells using simple culture conditions with endothelium induction agents in the medium.


Assuntos
Diferenciação Celular/fisiologia , Células Progenitoras Endoteliais/citologia , Células-Tronco Mesenquimais/citologia , Osteogênese/fisiologia , Geleia de Wharton/citologia , Técnicas de Cultura de Células , Proliferação de Células/fisiologia , Células Cultivadas , Citometria de Fluxo , Humanos
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