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1.
J Bacteriol ; 195(20): 4753-60, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23974026

RESUMO

Sulfate-reducing bacteria are characterized by a high number of hydrogenases, which have been proposed to contribute to the overall energy metabolism of the cell, but exactly in what role is not clear. Desulfovibrio spp. can produce or consume H2 when growing on organic or inorganic substrates in the presence or absence of sulfate. Because of the presence of only two hydrogenases encoded in its genome, the periplasmic HynAB and cytoplasmic Ech hydrogenases, Desulfovibrio gigas is an excellent model organism for investigation of the specific function of each of these enzymes during growth. In this study, we analyzed the physiological response to the deletion of the genes that encode the two hydrogenases in D. gigas, through the generation of ΔechBC and ΔhynAB single mutant strains. These strains were analyzed for the ability to grow on different substrates, such as lactate, pyruvate, and hydrogen, under respiratory and fermentative conditions. Furthermore, the expression of both hydrogenase genes in the three strains studied was assessed through quantitative reverse transcription-PCR. The results demonstrate that neither hydrogenase is essential for growth on lactate-sulfate, indicating that hydrogen cycling is not indispensable. In addition, the periplasmic HynAB enzyme has a bifunctional activity and is required for growth on H2 or by fermentation of pyruvate. Therefore, this enzyme seems to play a dominant role in D. gigas hydrogen metabolism.


Assuntos
Proteínas de Bactérias/metabolismo , Desulfovibrio gigas/enzimologia , Regulação Bacteriana da Expressão Gênica/fisiologia , Hidrogenase/classificação , Hidrogenase/metabolismo , Proteínas de Bactérias/genética , Desulfovibrio gigas/genética , Desulfovibrio gigas/metabolismo , Fermentação , Deleção de Genes , Regulação Enzimológica da Expressão Gênica/fisiologia , Hidrogênio/metabolismo , Hidrogenase/genética , Ácido Láctico/metabolismo , Dados de Sequência Molecular , Ácido Pirúvico/metabolismo , RNA Bacteriano/genética , RNA Bacteriano/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Transcriptoma
2.
Microbiologyopen ; 3(4): 513-30, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25055974

RESUMO

Desulfovibrio gigas is a model organism of sulfate-reducing bacteria of which energy metabolism and stress response have been extensively studied. The complete genomic context of this organism was however, not yet available. The sequencing of the D. gigas genome provides insights into the integrated network of energy conserving complexes and structures present in this bacterium. Comparison with genomes of other Desulfovibrio spp. reveals the presence of two different CRISPR/Cas systems in D. gigas. Phylogenetic analysis using conserved protein sequences (encoded by rpoB and gyrB) indicates two main groups of Desulfovibrio spp, being D. gigas more closely related to D. vulgaris and D. desulfuricans strains. Gene duplications were found such as those encoding fumarate reductase, formate dehydrogenase, and superoxide dismutase. Complexes not yet described within Desulfovibrio genus were identified: Mnh complex, a v-type ATP-synthase as well as genes encoding the MinCDE system that could be responsible for the larger size of D. gigas when compared to other members of the genus. A low number of hydrogenases and the absence of the codh/acs and pfl genes, both present in D. vulgaris strains, indicate that intermediate cycling mechanisms may contribute substantially less to the energy gain in D. gigas compared to other Desulfovibrio spp. This might be compensated by the presence of other unique genomic arrangements of complexes such as the Rnf and the Hdr/Flox, or by the presence of NAD(P)H related complexes, like the Nuo, NfnAB or Mnh.


Assuntos
DNA Bacteriano/química , DNA Bacteriano/genética , Desulfovibrio gigas/genética , Genoma Bacteriano , Análise de Sequência de DNA , Proteínas de Bactérias/genética , Análise por Conglomerados , Sequência Conservada , Variação Genética , Dados de Sequência Molecular , Filogenia
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