Volatile organic compound emissions induced by the aphid Myzus persicae differ among resistant and susceptible peach cultivars and a wild relative.

Little is known on aphid-induced emissions of volatile organic compounds (VOCs) from trees and particularly on their intraspecific variability in association with resistance traits. We compared VOC emissions from five peach cultivars (Prunus persica (L.) Batsch) and a wild relative (Prunus davidiana (Carrière) Franch) that differ in their level (susceptible/resistant) and type (antixenosis, antibiosis) of resistance to the green peach aphid, Myzus persicae (Sulzer). Additionally, the kinetics of VOC induction in response to aphids was compared with that by mechanical wounding. Qualitative and overall quantitative differences among peach genotypes were found in VOC emissions that were mainly composed of methyl-salicylate, farnesenes, (E)-ß-ocimene and (E)-4,8-dimethyl-1,3,7-nonatriene. Irrespective of the type of resistance, all resistant genotypes had increased VOC emissions upon aphid attack, while in susceptible genotypes emissions remained low. Emission increases were highest in the genotypes that express increased aphid resistance during second infestations, which had also the highest proportions of methyl-salicylate in their emissions. VOC induction by aphids proceeded slowly with a delay of several hours. Artificial wounding of leaves did not result in emissions of aphid-induced VOCs but caused an immediate burst of green leaf volatiles and benzaldehyde. We conclude that VOC induction in resistant peach cultivars is part of a general defence syndrome that is being avoided or suppressed by M. persicae in the susceptible genotypes. The induction likely involves an aphid-specific elicitor and (methyl)-salicylate in the subsequent signalling and regulation processes that should include gene activation due to the marked delay in the emission response. The results are compared with those of the literature and discussed in view of their ecological and environmental significance.

Chemical and growth traits of the peach tree may induce higher infestation rates of the green peach aphid, Myzus persicae (Sulzer).

BACKGROUND: The green peach aphids, Myzus persicae, are a predominant pest in peach orchards as they can alter fruiting and shoot development for several years. This aphid developed resistance against pesticides. Among the alternative control strategies is the reduction of the trees' attractiveness to aphids. In order to identify the plant variables related to plant susceptibility, young peach trees were submitted to various supplies of water and nitrogen, and then artificially infested with aphids. Shoot development, plant water potential and aphid abundance were then monitored on a weekly basis. The apex concentrations in total N, amino acids, soluble sugars and polyphenols were determined at infestation start and infestation peak. RESULTS: Until infestation peak, the thermal time requests for aphid development were independent of infestation severity. The aphid populations then collapsed more rapidly on the low infested shoots than on the high infested ones. Aphid abundances appeared to be positively related to shoot development (leaf expansion and secondary ramification), to shoot growth (stem length and diameter) and to apex concentrations in amino acids and non-structural carbohydrates (NSC). Polyphenols had the opposite effect. CONCLUSION: Peach susceptibility to aphids depends on shoot development and apex composition, and could be lowered by decreasing the water and nitrogen inputs. © 2019 Society of Chemical Industry.

Involvement of the xyloglucan endotransglycosylase/hydrolases encoded by celery XTH1 and Arabidopsis XTH33 in the phloem response to aphids.

During infestation, phloem-feeding insects induce transcriptional reprogramming in plants that may lead to protection. Transcripts of the celery XTH1 gene, encoding a xyloglucan endotransglycosylase/hydrolase (XTH), were previously found to accumulate systemically in celery (Apium graveolens) phloem, following infestation with the generalist aphid Myzus persicae. XTH1 induction was specific to the phloem but was not correlated with an increase in xyloglucan endotransglycosylase (XET) activity in the phloem. XTH1 is homologous to the Arabidopsis thaliana XTH33 gene. XTH33 expression was investigated following M. persicae infestation. The pattern of XTH33 expression is tightly controlled during development and indicates a possible role in cell expansion. An xth33 mutant was assayed for preference assay with M. persicae. Aphids settled preferentially on the mutant rather than on the wild type. This suggests that XTH33 is involved in protecting plants against aphids; therefore, that cell wall modification can alter the preference of aphids for a particular plant. Nevertheless, the ectopic expression of XTH33 in phloem tissue was not sufficient to confer protection, demonstrating that modifying the expression of this single gene does not readily alter plant-aphid interactions.