A review of the use of Duddingtonia flagrans as a biological controller of strongylid nematodes in horses.

In horses, the nematodes of the Strongylidae family are the most important due to their prevalence and pathogenicity. Sanitary plans include parasite control based on chemical anthelmintics. Among these, the benzimidazole compounds have been used since the 1960s to control the nematode Strongylus vulgaris. Its inappropriate use resulted in the development of resistance in parasites with a shorter biological cycle, such as the small strongyles. Currently, the genera that make up this group show widespread resistance to all chemical treatments available in veterinary medicine, except for macrocyclic lactones, where less effective action has been detected. The need to find alternative routes for its control is recognized. International organizations and markets are increasingly restrictive in the allowed levels of drugs in products of animal origin, so one of the drawbacks is the permanence of chemical compounds in tissues. Therefore, other tools not chemically based are proposed, such as the biological control of gastrointestinal nematodes. Various research groups around the world have carried out tests on the control capacity of the nematophagous fungus Duddingtonia flagrans against this group of parasites. The objective of this review is to compile the different tests that are available on biological control in this species, in in vivo and in vitro tests, and the possible incorporation of this tool as an alternative method of antiparasitic control in an integrated control scheme of parasites.

Predatory effect of Duddingtonia flagrans on infective larvae of gastro-intestinal parasites under sunny and shaded conditions.

Duddingtonia flagrans is a natural strain of Nematophagous-Fungi isolated around the world. It has demonstrated efficacy and ease of use in laboratory as well as in field conditions. The fungus contributes to the prophylactic control of the worms by reducing the number of L3 on pasture. The aims of this study were to test and analyze the predatory effect of D. flagrans under sunny and shaded conditions on the L3 in the faeces, and to verify the reduction of translation to pasture during summer and winter seasons. Faecal Mass Units (FMUs) were assigned to two treated groups (groups treated with D. flagrans chlamydospores, TG) and two untreated groups (without D. flagrans chlamydospores, UG), in summer and winter, under sunny and shaded conditions. FMUs and herbage samples were taken for parasitological workup. Predatory activity of D. flagrans was evident under both conditions for the summer experiment but was not manifest for the winter experiment. In summer, an interaction between sunny and shaded conditions and predatory activity of D. flagrans was found. Environmental conditions on predatory activity should be considered when designing strategies for the implementation of D. flagrans in grazing systems to smooth the infectivity curve of L3.

Nematophagous fungi from decomposing cattle faeces in Argentina.

BACKGROUND: Biological control of gastrointestinal nematodes of ruminants by use of nematophagous fungi would become part of any livestock parasite integral control system. Identifying autochthonous species that could then be selected for mass production is an important phase in the practical use of biological control. AIMS: To search for nematophagous fungi with potential use as biological control agents against gastrointestinal nematodes in Argentina. METHODS: Decomposing cattle faeces sampled in different locations were incubated in water agar 2% with Panagrellus sp. The developed nematophagous fungi were transferred to new water agar 2% plates and then to corn meal agar plates in order to carry out their identification. Fungal diversity and richness were also assessed. RESULTS: Seventeen species from nine genera of nematophagous fungi were found. Twelve species were nematode-trapping fungi and three species plus two fungi identified to genus level corresponded to endoparasitic fungi. Arthrobotrys conoides, Arthrobotrys oligospora, Duddingtonia flagrans, Monacrosporium doedycoides, Arthrobotrys robusta and Drechmeria coniospora were the most frequently isolated species overall in the whole study (6.6%, 5.7%, 5.7%, 5.7%, 4.7% and 4.7%, respectively) although other species were more frequently recorded at local levels such as Arthrobotrys pyriformis (18.8%). Only A. conoides has been previously isolated from ruminant faecal samples in Argentina. Five nematode-trapping fungal species are mentioned for the first time in the Americas CONCLUSIONS: D. flagrans and A. conoides, both identified in the present study, are among the most promising ones as biological control agents against gastrointestinal nematodes of ruminants.