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1.
Sensors (Basel) ; 16(1)2015 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-26703624

RESUMO

In this work we show the principle of optical 3D surface measurements based on the fringe projection technique for underwater applications. The challenges of underwater use of this technique are shown and discussed in comparison with the classical application. We describe an extended camera model which takes refraction effects into account as well as a proposal of an effective, low-effort calibration procedure for underwater optical stereo scanners. This calibration technique combines a classical air calibration based on the pinhole model with ray-based modeling and requires only a few underwater recordings of an object of known length and a planar surface. We demonstrate a new underwater 3D scanning device based on the fringe projection technique. It has a weight of about 10 kg and the maximal water depth for application of the scanner is 40 m. It covers an underwater measurement volume of 250 mm × 200 mm × 120 mm. The surface of the measurement objects is captured with a lateral resolution of 150 µm in a third of a second. Calibration evaluation results are presented and examples of first underwater measurements are given.


Assuntos
Monitoramento Ambiental/métodos , Imageamento Tridimensional/métodos , Algoritmos , Monitoramento Ambiental/instrumentação , Hidrobiologia , Imageamento Tridimensional/instrumentação , Água/química
2.
J Hand Surg Glob Online ; 6(3): 409-414, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38817750

RESUMO

We report a rare case in which the entire capitate was replaced by free nonvascularized autologous bone grafting due to a giant cell tumor. Moreover, a Maestro wrist prosthesis (second type) was subsequently implanted after two failed motion-preserving procedures. At the 6- and 8-year follow-ups, we observed complete but asymptomatic avascular bone necrosis around the entire capitate peg without evident failure of the carpal component. This case highlights the importance of locking screws in improving the longevity of total wrist arthroplasty by imitating external or internal fixation for bridging large bony defects in the long bones of the upper and lower extremities. However, loosening may occur in the future. To the best of our knowledge, this is the first report to describe an uncommon finding that cannot be solely attributed to the natural course of periprosthetic osteolysis.

3.
J Clin Med ; 12(5)2023 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-36902704

RESUMO

OBJECTIVES: Low-field MRI at 0.55 Tesla (T) with deep learning image reconstruction has recently become commercially available. The objective of this study was to evaluate the image quality and diagnostic reliability of knee MRI performed at 0.55T compared with 1.5T. METHODS: A total of 20 volunteers (9 female, 11 male; mean age = 42 years) underwent knee MRI on a 0.55T system (MAGNETOM Free.Max, Siemens Healthcare, Erlangen, Germany; 12-channel Contour M Coil) and a 1.5T scanner (MAGNETOM Sola, Siemens Healthcare, Erlangen, Germany; 18-channel transmit/receive knee coil). Standard two-dimensional (2D) turbo spin echo (TSE), fat-suppressed (fs) proton density-weighted (PDw), T1w TSE, and T2w TSE sequences were acquired in approximately 15 min. In total, 2 radiologists blinded to the field strength subjectively assessed all MRI sequences (overall image quality, image noise, and diagnostic quality) using a 5-point Likert scale (1-5; 5 = best). Additionally, both radiologists evaluated the possible pathologies of menisci, ligaments, and cartilage. Contrast ratios (CRs) of different tissues (bone, cartilage, and menisci) were determined on coronal PDw fs TSE images. The statistical analysis included Cohen's kappa and the Wilcoxon rank sum test. RESULTS: The overall image quality of the 0.55T T2w, T1w, and PDw fs TSE sequences was diagnostic and rated similar for T1w (p > 0.05), but lower for PDw fs TSE and T2w TSE compared with 1.5T (p < 0.05). The diagnostic accordance of meniscal and cartilage pathologies at 0.55T was similar to 1.5T. The CRs of the tissues were not significantly different between 1.5T and 0.55T (p > 0.05). The inter-observer agreement of the subjective image quality was generally fair between both readers and almost perfect for the pathologies. CONCLUSIONS: Deep learning-reconstructed TSE imaging at 0.55T yielded diagnostic image quality for knee MRI compared with standard 1.5T MRI. The diagnostic performance of meniscal and cartilage pathologies was equal for 0.55T and 1.5T without a significant loss of diagnostic information.

4.
Tomography ; 8(4): 1666-1675, 2022 06 23.
Artigo em Inglês | MEDLINE | ID: mdl-35894003

RESUMO

The purpose of this study was the evaluation of image quality and radiation dose parameters of the novel photon counting CT (PCCT, Naeotom Alpha, Siemens Healthineers) using low-dose scan protocols for the detection of urolithiasis. Standard CT scans were used as a reference (S40, Somatom Sensation 40, Siemens Healthineers). Sixty-three patients, who underwent CT scans between August and December 2021, were retrospectively enrolled. Thirty-one patients were examined with the PCCT and 32 patients were examined with the S40. Radiation dose parameters, as well as quantitative and qualitative image parameters, were analyzed. The presence of urolithiasis, image quality, and diagnostic certainty were rated on a 5-point-scale by 3 blinded readers. Both patient groups (PCCT and S40) did not differ significantly in terms of body mass index. Radiation dose was significantly lower for examinations with the PCCT compared to the S40 (2.4 ± 1.0 mSv vs. 3.4 ± 1.0 mSv; p < 0.001). The SNR was significantly better on images acquired with the PCCT (13.3 ± 3.3 vs. 8.2 ± 1.9; p < 0.001). The image quality of the PCCT was rated significantly better (4.3 ± 0.7 vs. 2.8 ± 0.6; p < 0.001). The detection rate of kidney or ureter calculi was excellent with both CT scanners (PCCT 97.8% and S40 99%, p = 0.611). In high contrast imaging, such as the depiction of stones of the kidney and the ureter, PCCT allows a significant reduction of radiation dose, while maintaining excellent diagnostic confidence and image quality. Given this image quality with our current protocol, further adjustments towards ultra-low-dose CT scans appear feasible.


Assuntos
Tomografia Computadorizada por Raios X , Urolitíase , Humanos , Doses de Radiação , Estudos Retrospectivos , Tomógrafos Computadorizados , Tomografia Computadorizada por Raios X/métodos , Urolitíase/diagnóstico por imagem
5.
Diagnostics (Basel) ; 12(6)2022 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-35741116

RESUMO

BACKGROUND: The purpose of the present study was the evaluation of the image quality of polyenergetic and monoenergetic reconstructions (PERs and MERs) of CT angiographies (CTAs) of the head and neck acquired with the novel photon counting CT (PCCT) method in clinical routine. METHODS: Thirty-seven patients were enrolled in this retrospective study. Quantitative image parameters of the extracranial, intracranial and cerebral arteries were evaluated for the PER and MER (40-120 keV). Additionally, two radiologists rated the perceived image quality. RESULTS: The mean CTDIvol used in the PCCT was 8.31 ± 1.19 mGy. The highest signal within the vessels was detected in the 40 keV MER, whereas the lowest noise was detected in the 115 keV MER. The most favorable contrast-to-noise-ratio (CNR) and signal-to-noise-ratio (SNR) were detected in the PER and low keV MER. In the qualitative image analysis, the PER was superior to the MER in all rated criteria. For MER, 60-65 keV was rated as best image quality. CONCLUSION: Overall, PCCT offers excellent image quality for CTAs of the head and neck. At the current state, the PER of the PCCT seems to be the most favorable reconstruction for diagnostic reporting.

6.
Plast Reconstr Surg Glob Open ; 9(2): e3424, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33680672

RESUMO

A 79-year-old man sustained left open metacarpophalangeal joints III-V dislocation injury in the dorsal direction associated with avulsions of the volar fibrocartilaginous plates. Surgical revision became necessary. Three months after the injury, the patient was very satisfied with his intermedium outcome; however, complete restoration of hand function was not observed at this time. To our knowledge, this is the first case presentation in the literature that describes such an injury involving the metacarpophalangeal joints III-V.

7.
Adv Mater ; 33(37): e2008617, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34338367

RESUMO

Shape morphing implicates that a specific condition leads to a morphing reaction. The material thus transforms from one shape to another in a predefined manner. In this paper, not only the target shape but rather the evolution of the material's shape as a function of the applied strain is programmed. To rationalize the design process, concepts from informatics (processing functions, for example, Poisson's ratio (PR) as function of strain: ν = f(ε) and if-then-else conditions) will be introduced. Three types of shape morphing behavior will be presented: (1) achieving a target shape by linearly increasing the amplitude of the shape, (2) filling up a target shape in linear steps, and (3) shifting a bulge through the material to a target position. In the first case, the shape is controlled by a geometric gradient within the material. The filling kind of behavior was implemented by logical operations. Moreover, programming moving hillocks (3) requires to implement a sinusoidal function εy  = sin (εx ) and an if-then-else statement into the unit cells combined with a global stiffness gradient. The three cases will be used to show how the combination of mechanical mechanisms as well as the related parameter distribution enable a programmable shape morphing behavior in an inverse design process.

8.
Biometals ; 23(4): 613-22, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20204476

RESUMO

Soluble ammonia monooxygenase (AMO) from Nitrosomonas europaea was purified to homogeneity and metals in the active sites of the enzyme (Cu, Fe) were analyzed by electron paramagnetic resonance (EPR) spectroscopy. EPR spectra were obtained for a type 2 Cu(II) site with g(parallel) = 2.24, A(parallel) = 18.4 mT and g(perpendicular) = 2.057 as well as for heme and non heme iron present in purified soluble AMO from N. europaea. A second type 2 Cu(II) EPR signal with g(parallel) = 2.29, A(parallel) = 16.1 mT and g(perpendicular) = 2.03 appeared in the spectrum of the ferricyanide oxidized enzyme and was attributed to oxidation of cuprous sites. Comparison of EPR-detectable Cu(2+) with total copper determined by inductively coupled plasma-mass spectrometry (ICP-MS) suggests that there are six paramagnetic Cu(2+) and three diamagnetic Cu(1+) per heterotrimeric soluble AMO (two paramagnetic and one diamagnetic Cu per alphabetagamma-protomer). A trigonal EPR signal at g = 6.01, caused by a high-spin iron, indicative for cytochrome bound iron, and a rhombic signal at g = 4.31, characteristic of specifically bound Fe(3+) was detectable. The binding of nitric oxide in the presence of reductant resulted in a ferrous S = 3/2 signal, characteristic of a ferrous nitrosyl complex. Inactivation of soluble AMO with acetylene did neither diminish the ferrous signal nor the intensity of the Cu(2+)-EPR signal.


Assuntos
Cobre/química , Espectroscopia de Ressonância de Spin Eletrônica/métodos , Ferro/química , Nitrosomonas europaea/enzimologia , Oxirredutases/química , Domínio Catalítico , Oxirredutases/metabolismo
9.
J Mech Behav Biomed Mater ; 110: 103933, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32957227

RESUMO

Explant analysis can provide key insights to understanding failures of artificial joints and thus how they might be improved for the ultimate benefit of patients. There are no previous reports of explant analysis of an artificial wrist joint. In this study, an explanted metal-on-polymer Maestro wrist was analysed both in macro and nanoscales to estimate its biotribological performance. The articulation was formed between a cobalt chromium carpal head and an ultrahigh molecular weight polyethylene bearing. The surface roughness values of its articulating surfaces and the backside of the articulation were measured. On average, the articulating surface roughness values were calculated as 0.06 ± 0.02 µm and 1.29 ± 0.63 µm for the cobalt chromium carpal head and ultrahigh molecular weight polyethylene bearing, respectively. Both surfaces had negative skewness, indicating a preponderance of valleys. On the articulating surface of the carpal head, light scratches were observed, and no impingement was observed throughout the component. The polymeric surface had a polished appearance. It had unidirectional scratches at the centre of the articulation, pits of different sizes on its articulating surface, and matt white subsurface regions. The backside of the UHMWPE bearing and the convex surface of the radial body that it was sitting on, were found to have average surface roughness values of 4.23 ± 0.69 µm and 5.57 ± 1.05 µm, respectively. The difference in the means was not significant (p > 0.05). Taking the articulating surface roughness values, the lubrication regime that the explanted Maestro wrist operated under in vivo was estimated for varying physiological conditions, i.e. varying loads, entraining velocities and lubricant viscosities. In every case considered, the explant was found to operate under boundary lubrication.


Assuntos
Prótese de Quadril , Prótese Articular , Análise de Falha de Equipamento , Humanos , Lubrificação , Desenho de Prótese , Falha de Prótese , Propriedades de Superfície , Punho , Articulação do Punho
10.
Acta Biomater ; 104: 221-230, 2020 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-31926334

RESUMO

During human skeletal growth, bone is formed via different processes. Two of them are: new bone formation by depositing bone at the periosteal (outer) surface and bone remodeling corresponding to a local renewal of tissue. Since in remodeling formation is preceded by resorption, we hypothesize that modeling and remodeling could require radically different transport paths for ionic precursors of mineralization. While remodeling may recycle locally resorbed mineral, modeling implies the transport over large distances to the site of bone apposition. Therefore, we searched for potential differences of size, arrangement and chemical composition of mineral particles just below surfaces of modeling and remodeling sites in femur midshaft cross-sections from healthy children. These bone sites were mapped using scanning synchrotron X-ray scattering, Raman microspectroscopy, energy dispersive X-ray analysis and quantitative backscattered electron microscopy. The results show clear differences in mineral particle size and composition between the sites, which cannot be explained by a change in the rate of mineral apposition or accumulation. At periosteal modeling sites, mineral crystals are distinctly larger, display higher crystallinity and exhibit a lower calcium to phosphorus ratio and elevated Na and Mg content. The latter may originate from Mg used for phase stabilization of mineral precursors and therefore indicate different time periods for mineral transport. We conclude that the mineralization process is distinctively different between modeling and remodeling sites due to varying requirements for the transport distance and, therefore, the stability of non-crystalline ionic precursors, resulting in distinct compositions of the deposited mineral phase. STATEMENT OF SIGNIFICANCE: In growing children new bone is formed either due to apposition of bone tissue e.g. at the outer ridge of long bones to allow growth in thickness (bone modeling), or in cavities inside the mineralized matrix when replacing tissue (bone remodeling). We demonstrate that mineral crystal shape and composition are not the same between these two sites, which is indicative of differences in mineralization precursors. We suggest that this may be due to a longer mineral transport distance to sites of new bone formation as compared to remodeling where mineral can be locally recycled.


Assuntos
Osso e Ossos/fisiologia , Calcificação Fisiológica , Osteogênese , Feminino , Humanos , Lactente , Masculino , Tamanho da Partícula , Espalhamento a Baixo Ângulo , Espectrometria por Raios X , Análise Espectral Raman , Síncrotrons , Difração de Raios X
11.
Biol Chem ; 390(9): 863-73, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19453274

RESUMO

Ammonia monooxygenase (AMO) of Nitrosomonas europaea is a metalloenzyme that catalyzes the oxidation of ammonia to hydroxylamine. This study shows that AMO resides in the cytoplasm of the bacteria in addition to its location in the membrane and is distributed approximately equally in both subcellular fractions. AMO in both fractions catalyzes the oxidation of ammonia and binds [(14)C]acetylene, a mechanism-based inhibitor which specifically interacts with catalytically active AMO. Soluble AMO was purified 12-fold to electrophoretic homogeneity with a yield of 8%. AMO has a molecular mass of approximately 283 kDa with subunits of ca. 27 kDa (alpha-subunit, AmoA), ca. 42 kDa (beta-subunit, AmoB), and ca. 24 kDa (gamma-subunit, cytochrome c(1)) in an alpha(3)beta(3)gamma(3) sub-unit structure. Different from the beta-subunit of membrane-bound AMO, AmoB of soluble AMO possesses an N-terminal signal sequence. AMO contains Cu (9.4+/-0.6 mol per mol AMO), Fe (3.9+/-0.3 mol per mol AMO), and Zn (0.5 to 2.6 mol per mol AMO). Upon reduction the visible absorption spectrum of AMO reveals absorption bands characteristic of cytochrome c. Electron para-magnetic resonance spectroscopy of air-oxidized AMO at 50 K shows a paramagnetic signal originating from Cu(2+) and at 10 K a paramagnetic signal characteristic of heme-Fe.


Assuntos
Proteínas de Bactérias/metabolismo , Nitrosomonas europaea/enzimologia , Oxirredutases/metabolismo , Acetileno/metabolismo , Proteínas de Bactérias/química , Membrana Celular/metabolismo , Citocromos c/química , Espectroscopia de Ressonância de Spin Eletrônica , Eletroforese , Oxirredutases/química , Ligação Proteica , Solubilidade
12.
Curr Microbiol ; 59(2): 130-8, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19452213

RESUMO

The ammonia oxidizers Nitrosomonas europaea and Nitrosomonas eutropha are able to grow chemoorganotrophically under anoxic conditions with pyruvate, lactate, acetate, serine, succinate, alpha-ketoglutarate, or fructose as substrate and nitrite as terminal electron acceptor. The growth yield of both bacteria is about 3.5 mg protein (mmol pyruvate)(-1) and the maximum growth rates of N. europaea and N. eutropha are 0.094 d(-1) and 0.175 d(-1), respectively. In the presence of pyruvate and CO2 about 80% of the incorporated carbon derives from pyruvate and about 20% from CO2. Pyruvate is used as energy and only carbon source in the absence of CO2 (chemoorganoheterotrophic growth). CO2 stimulates the chemoorganotrophic growth of both ammonia oxidizers and the expression of ribulose bisphosphate carboxylase/oxygenase is down-regulated at increasing CO2 concentration. Ammonium, although required as nitrogen source, is inhibitory for the chemoorganotrophic metabolism of N. europaea and N. eutropha. In the presence of ammonium pyruvate consumption and the expression of the genes aceE, ppc, gltA, odhA, and ppsA (energy conservation) as well as nirK, norB, and nsc (denitrification) are reduced.


Assuntos
Nitritos/metabolismo , Nitrosomonas/crescimento & desenvolvimento , Nitrosomonas/metabolismo , Compostos Orgânicos/metabolismo , Amônia/metabolismo , Anaerobiose , Carbono/metabolismo , Regulação Bacteriana da Expressão Gênica , Nitrosomonas europaea/crescimento & desenvolvimento , Nitrosomonas europaea/metabolismo
13.
Methods Enzymol ; 440: 121-35, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18423214

RESUMO

The biological nitrogen cycle is a complex interplay of many microorganisms. In the past, oxidation of the inorganic nitrogen compound ammonia by the ammonia oxidizing bacteria was thought to be restricted to oxic environments, and the metabolic flexibility of these organisms seemed to be limited. The discovery of an anaerobic metabolism in the late 1990s showed that these assumptions are no longer valid. NO and NO(2) are essential intermediates in ammonia oxidation. Both gases have wide-ranging regulatory effects on ammonia oxidation, denitrification, and biofilm formation of the ammonia oxidizing bacteria.


Assuntos
Amônia/metabolismo , Óxido Nítrico/metabolismo , Nitrosomonas europaea/enzimologia , Oxirredutases/metabolismo , Aerobiose , Anaerobiose , Nitrosomonas europaea/metabolismo , Oxirredução
14.
Open Orthop J ; 12: 180-189, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29997705

RESUMO

BACKGROUND: A devasting course of Iliopsoas Muscle (IPM) abscess remains a challenging therapeutic problem. METHODS: A 69-year-old polymorbid male had a history of multiple carcinomas and presented with advanced stage of septic shock due to a right IPM abscess which communicated with the right hip joint and subsequently led to septic hip arthritis accompanied with post-infectious right gluteal deep soft tissue necroses. Management of surgical treatment included abscess revision, coverage with the use of Long Head Biceps Femoris Muscle (LHBFM) 180° turnover flap, and creating a Girdlestone resection-arthroplasty. RESULTS: After a duration of patient's hospitalization of six months that included the necessity of artificial respiration over two months accompanied with in summary 18 required surgical procedures, the patient could be recovered successfully regarding his polymorbidity and his low-demand claims in activities of daily living with his Girdlestone resection-arthroplasty. CONCLUSION: Recovery of immunocompromised patients with those life-threatening situations can only be achieved by an interdisciplinary management. The LHBFM 180° turnover flap can be useful for filling off post-infectious deep soft tissue cavities communicating with the hip joint. The definitive Girdlestone resection-arthroplasty for treatment of septic hip arthritis is the method of choice for mobilization of elderly polymorbid patients with low demand claims in their activities of daily living.

15.
J Mech Behav Biomed Mater ; 77: 258-266, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-28957701

RESUMO

All hierarchical levels in bone are known to contribute to its mechanical behavior. The basic building block is the mineralized collagen fibril which is assembled into larger structures with varying fibrillar organization. The collagen organization increases from unordered woven bone in the callus which is gradually replaced by higher ordered lamellar bone during bone development and healing and finally results in cortical lamellar bone with highest degree of organization. The structural and mechanical description of these organizational motifs is not yet complete. We investigated a femoral osteotomy mouse model and analyzed newly formed callus tissue and mature lamellar bone in the cortex. This model exhibits three bone types with different fibrillar organization: (i) woven, (ii) moderate lamellar and (iii) lamellar. Using high resolution synchrotron small angle X-ray scattering in combination with back-scattered electron imaging we characterized the ultrastructure of the different regions in terms of degree of mineralization, averaged mineral particle thickness and mineral particle orientation. We further used microindentation to correlate hardness, induced crack lengths and crack patterns with the bone ultrastructure. The newly formed callus tissue contains highly mineralized woven bone islands, featuring thick but poorly ordered mineral particles. Such islands are surrounded by layers of lamellar bone with a low mineralization level and thin but well aligned particles. Callus tissue shows lower hardness values and longer cracks than the cortex. Callus woven bone exhibits shorter cracks than callus lamellar bone. However, the poorly mineralized callus lamellar bone shows crack propagation mechanisms similar to cortical bone due to its very similar lamellar organization and high degree of mineral particle orientation. In conclusion we demonstrate that woven and increasingly higher oriented lamellar bone do not only differ in collagen fibril organization, but also that the amount, orientation and different shape of mineral particles are also likely to contribute to the reduced mechanical competence of woven as compared to lamellar bone. This may explain why many organisms replace less organized bone types with higher organized ones.


Assuntos
Osso e Ossos/patologia , Osso e Ossos/fisiologia , Consolidação da Fratura , Fraturas Ósseas/fisiopatologia , Teste de Materiais , Animais , Calcificação Fisiológica , Colágeno/química , Feminino , Dureza , Camundongos , Camundongos Endogâmicos C57BL , Microscopia Eletrônica , Modelos Animais , Nanoestruturas/química , Osteotomia , Espalhamento de Radiação , Espalhamento a Baixo Ângulo , Estresse Mecânico , Síncrotrons
16.
J Bacteriol ; 189(24): 9090-100, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17921289

RESUMO

The family of ammonia and ammonium channel proteins comprises the Amt proteins, which are present in all three domains of life with the notable exception of vertebrates, and the homologous Rh proteins (Rh50 and Rh30) that have been described thus far only in eukaryotes. The existence of an RH50 gene in bacteria was first revealed by the genome sequencing of the ammonia-oxidizing bacterium Nitrosomonas europaea. Here we have used a phylogenetic approach to study the evolution of the N. europaea RH50 gene, and we show that this gene, probably as a component of an integron cassette, has been transferred to the N. europaea genome by horizontal gene transfer. In addition, by functionally characterizing the Rh50(Ne) protein and the corresponding knockout mutant, we determined that NeRh50 can mediate ammonium uptake. The RH50(Ne) gene may thus have replaced functionally the AMT gene, which is missing in the genome of N. europaea and may be regarded as a case of nonorthologous gene displacement.


Assuntos
Amônia/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Nitrosomonas europaea/genética , Nitrosomonas europaea/fisiologia , Evolução Molecular , Deleção de Genes , Filogenia , Homologia de Sequência de Aminoácidos
17.
FEMS Microbiol Lett ; 273(2): 260-7, 2007 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17608700

RESUMO

Ammonium transporters form a conserved family of transport proteins and are widely distributed among all domains of life. The genome of Nitrosomonas europaea codes for a single gene (rh1) that belongs to the family of the AMT/Rh ammonium transporters. For the first time, this study provides functional and physiological evidence for a rhesus-type ammonia transporter in bacteria (N. europaea). The methylammonium (MA) transport activity of N. europaea correlated with the Rh1 expression. The K(m) value for the MA uptake of N. europaea was 1.8+/-0.2 mM (pH 7.25), and the uptake was competitively inhibited by ammonium [K(i)(NH(4) (+)) 0.3+/-0.1 mM at pH 7.25]. The MA uptake rate was pH dependent, indicating that the uncharged form of MA is transported by Rh1. An effect of the glutamine synthetase on the MA uptake was not observed. When expressed in Saccharomyces cerevisiae, the function of Rh1 from N. europaea as an ammonia/MA transporter was confirmed. The results suggest that Rh1 equilibrates the uncharged substrate species. A low pH value in the periplasmic space during ammonia oxidation seems to be responsible for the ammonium accumulation functioning as an acid NH(4) (+) trap.


Assuntos
Proteínas de Bactérias/fisiologia , Proteínas de Transporte de Cátions/fisiologia , Nitrosomonas europaea/metabolismo , Compostos de Amônio Quaternário/metabolismo , Acetileno/farmacologia , Proteínas de Bactérias/classificação , Proteínas de Bactérias/genética , Radioisótopos de Carbono , Proteínas de Transporte de Cátions/classificação , Proteínas de Transporte de Cátions/genética , Genoma Bacteriano , Transporte de Íons , Metilaminas/metabolismo , Nitrosomonas europaea/efeitos dos fármacos , Oxirredução , Compostos de Amônio Quaternário/farmacologia , RNA Mensageiro/metabolismo
18.
Open Orthop J ; 11: 794-803, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28979592

RESUMO

BACKGROUND: Non-traumatic radial nerve palsy (RNP) caused by local tumors is a rare and uncommon entity. METHODS: A 62-year-old female presented with a left non-traumatic RNP, initially starting with weakness only. It was caused by a benign giant lipoma at the proximal forearm that was misdiagnosed over a period of 2 years. The slowly growth of the tumor led to an irreparable overstretching-related partial nerve disruption. For functional recovery of the patient, a triple tendon transfer procedure had to be performed. RESULTS: Four months after surgery, the patient was completely able to perform her activities of daily living again. At the 10-months follow-up, strength of wrist extension, thumb's extension and abduction, and long fingers II-V extension had all improved to grade 4 in Medical Research Council scale (0-5). In order to restore motion, the patient reported that she would undergo the same triple tendon transfer procedure a second time where necessary. Due to the initially misdiagnosed tumor, there was an overall delayed duration of time for functional recovery of the patient. CONCLUSION: The triple tendon transfer procedure offers a useful and reliable method to restore functionality for patients sustaining irreparable RNP. However, it must be noted critically with our patient that this procedure probably would have been avoided. Initially, there was weakness only by entrapment of the radial nerve. RNP caused by local tumors are uncommon but known from the literature, and so it should be considered generally in differential diagnosis of non-traumatic RNP.

19.
Open Orthop J ; 11: 248-254, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28567152

RESUMO

BACKGROUND: Coronal shear fracture type IV of the distal part of humerus is a very rare injury with articular complexity potentially leading to posttraumatic osteoarthritis. One option for surgical treatment of advanced unicompartmental radiocapitellar osteoarthritis is resurfacing radiocapitellar joint replacement. METHOD: A 62-year- old female sustained a coronal shear fracture type IV of the distal part of left humerus that was primarily treated with open reduction and internal fixation using headless compression screws. Three years postoperatively, there was a migration of one screw into radiocapitellar joint that led to circular deep cartilage defect of radial head. Four years after ORIF, a distinctive radiocapitellar osteoarthritis has evolved leading to a resurfacing radiocapitellar joint replacement using the Lateral Resurfacing ElbowTM (LRE) system. RESULT: At the 2-year follow-up after that procedure, there was an excellent subjective and functional outcome. Radiographically, no loosening or subsidence of implant without any signs of overstuffing could be found. The patient reported that she would have the same procedure again. CONCLUSION: The goal of unicompartmental radiocapitellar replacement is to obtain stability in elbow joint by avoiding cubitus valgus with subsequent instability of the distal radioulnar joint, and it does not alter the unaffected ulnohumeral joint. Additionally, the feature of the LRETM system is that the radial head is not excised, and so will receive the anatomical length of the overall radius articulating with the capitellum by preserving the annular ligament. In the literature only three publications could be found in which short-term results with the use of the LRETM system have been described. Hence, further studies are needed to validate this concept.

20.
Open Orthop J ; 11: 439-446, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28660002

RESUMO

BACKGROUND: Posttraumatic ulnar carpal translocation is a very rare condition that is caused either by fracture-dislocation injury or by purely ligamentous injury of the wrist. Its prognosis is poor and development of posttraumatic pancarpal wrist joint osteoarthritis is inevitable, and options for treatment are total wrist fusion or total wrist arthroplasty. METHODS: A 24-year-old male sustained a fracture-related injury in his left wrist that was accompanied with a second ligamentous distorsion-related injury 1 year later in the same wrist. Seven years after first injury, a posttraumatic pancarpal wrist joint osteoarthritis has developed that was caused by posttraumatic ulnar carpal translocation. The patient was treated by total wrist arthroplasty with use of the MaestroTM Wrist Reconstructive System. RESULTS: With our patient, it is unclear whether posttraumatic ulnar carpal translocation occurred either as result of the first fracture-related injury or as result of the second ligamentous distorsion-related injury or as result of both injuries. The 31-year-old patient could be reemployed completely in his original occupation as a mechanic for big agriculture machines and load his wrist with more than 10 pounds. In order to preserve motion, the patient reported that he would undergo the same total wrist arthroplasty a second time were it necessary. CONCLUSION: We report on a young male receiving total wrist arthroplasty and resulting in good restoration of his high-demand claims in activities of daily living, respectively. However, it cannot be concluded that total wrist arthroplasty is to be preferred generally over total wrist fusion in young patients. Essential prerequisite for this motion-preserving procedure is the compliance of patients.

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