Assuntos
Distrofia Miotônica/genética , Animais , Linhagem Celular , Mapeamento Cromossômico , Cromossomos Humanos Par 19/ultraestrutura , Cricetinae , Cricetulus , DNA/genética , DNA Recombinante , Genes Dominantes , Ligação Genética , Marcadores Genéticos , Humanos , Camundongos , Linhagem , Polimorfismo de Fragmento de RestriçãoRESUMO
A panel of human-rodent somatic cell hybrids containing different regions of chromosome 19 has been used to obtain a regional localization for peptidase D. The results assign PEPD to the long arm of chromosome 19, in the region cen-q13.2
Assuntos
Cromossomos Humanos Par 19 , Dipeptidases/genética , Animais , Mapeamento Cromossômico , DNA , Marcadores Genéticos , Humanos , Hibridização de Ácido NucleicoRESUMO
We have cloned and characterized two distinct types of alphoid DNA elements. Probe pG-Xba 11/340 was obtained by random cloning of human satellite DNA and contains two basic units with overall 88% homology to the 171-bp consensus alphoid sequence. pG-Xba 11/340-like elements are represented about 2,000-4,000 times in the haploid genome and, by in situ hybridization, are found exclusively at the primary constrictions of chromosomes 4 and 9. Probe pG-A16 was cloned from a chromosome 19-specific cosmid library and represents a 2.25-kb higher-order DNA element which is present at roughly 75-150 copies per haploid genome and which hybridizes to the centromeres of chromosomes 5 and 19. Using the pG-A16 probe, further genetic and physical dissection of the central area of chromosome 19 can be envisaged.
Assuntos
Sequência de Bases , Cromossomos Humanos 4-5 , Cromossomos Humanos Par 19 , Cromossomos Humanos Par 9 , DNA Satélite/genética , Homologia de Sequência do Ácido Nucleico , Centrômero , Clonagem Molecular , Enzimas de Restrição do DNA , Humanos , Dados de Sequência Molecular , Hibridização de Ácido NucleicoRESUMO
Pregnancy specific beta-1-glycoprotein (PSBG), a major product of the human placenta, is encoded by multiple genes. Southern blot hybridization of human x rodent somatic cell hybrid DNAs with a cDNA specific for one member of the PSBG gene family allowed us to map this gene to human chromosome 19. Further analysis using hybrids with subchromosomal segments of 19q revealed that the gene maps to the distal segment of region 19q13.1.
Assuntos
Cromossomos Humanos Par 19 , Proteínas da Gravidez/genética , Glicoproteínas beta 1 Específicas da Gravidez/genética , Animais , Southern Blotting , Antígeno Carcinoembrionário/genética , Mapeamento Cromossômico , Sondas de DNA , Humanos , Células Híbridas , Família MultigênicaRESUMO
The antigen recognized by the monoclonal antibody Ki-67 is a proliferation-related nucleolus-associated constituent used as a marker for cycling cells in tumor diagnosis. Antibody Ki-67 reacts with human proliferating cells, but not with hamster and mouse cells. Expression of the Ki-67 antigen was studied in a panel of human-rodent somatic cell hybrids. The results indicate that a gene involved in the expression of the antigen is located on chromosome 10.
Assuntos
Cromossomos Humanos Par 10 , Expressão Gênica , Proteínas Nucleares/genética , Animais , Anticorpos Monoclonais , Mapeamento Cromossômico , Humanos , Células Híbridas , Immunoblotting , Antígeno Nuclear de Célula em ProliferaçãoRESUMO
The localization to 19q of the gene causing myotonic dystrophy (DM) has been defined more precisely by refinement of the physical location of several linked markers. A somatic cell hybrid mapping panel from cells with t(1;19), t(12;19), and t(X;19) translocation products was constructed to define five different intervals across 19q. In addition, we have derived a series of cell hybrids by irradiation of a der(19)-only hybrid to further subdivide the cen-q13.1 region. Using an array of 36 cloned genes, anonymous DNAs, and enzyme markers, we have tested the location of the panel breakpoints and refined the regional assignment of several of these markers. All markers tightly linked to DM are localized mainly within 19q13.2, thus suggesting that the DM gene is also close to this region.
Assuntos
Cromossomos Humanos Par 19/ultraestrutura , Distrofia Miotônica/genética , Animais , Mapeamento Cromossômico , Cricetinae , Sondas de DNA , Marcadores Genéticos , Humanos , Células Híbridas , Camundongos , Hibridização de Ácido Nucleico , Homologia de Sequência do Ácido Nucleico , Especificidade da EspécieRESUMO
Hybridization studies using a panel of somatic cell hybrids with subchromosomal segments of 19q have localized the genes encoding hormone-sensitive lipase (LIPE), carcinoembryonic antigen (CEA), and small nuclear ribonucleoprotein polypeptide A (SNRPA) to various regions of 19q13.1; the cellular receptor for poliovirus sensitivity (PVS) to 19q13.2; and the genes coding for prostate-specific antigen (APS), human pancreatic kallikrein (KLK1), and small nuclear ribonucleoprotein 70-kD polypeptide (SNRP70) to 19q13.3----qter. Our results exclude several of these genes from being seriously considered as a candidate for the myotonic dystrophy gene on 19q.
Assuntos
Mapeamento Cromossômico , Cromossomos Humanos Par 19 , Distrofia Miotônica/genética , Animais , Antígenos de Neoplasias/genética , Southern Blotting , Antígeno Carcinoembrionário/genética , Genes , Ligação Genética , Marcadores Genéticos , Humanos , Células Híbridas , Calicreínas/genética , Lipase/genética , Masculino , Camundongos , Poliovirus , Antígeno Prostático Específico , Neoplasias da Próstata/genética , Receptores Virais/genética , Ribonucleoproteínas/genética , Ribonucleoproteínas Nucleares PequenasRESUMO
The recently developed competitive in situ hybridization (CISH) strategy was applied to the analysis of chromosome 12 aberrations in testicular germ cell tumors (TGCTs). DNAs from two rodent-human somatic cell hybrids, containing either a normal chromosome 12 or the p arm of chromosome 12 as their unique human material, were used as probes. Our results demonstrate a genuine iso-12p character of the standard marker chromosome in TGCTs. Moreover, variant markers were identified representing translocation products that also involve chromosome 12.