RESUMO
INTRODUCTION: Viral hepatitis B is a global scourge affecting millions of people worldwide. In Morocco, hepatitis B is considered a public health problem, and available data converge to consider Morocco as a country with intermediate endemicity. In the present study, we have planned to evaluate the HBV prevalence in Morocco on a large scale and to assess the prevalence of different serological markers for better management of this infection in Morocco. METHODS: This study was conducted on 18,877 patients referring to the Ibn Sina University Hospital Center of Rabat, Morocco. HBV serological markers including HBsAg, HBsAb, HBeAg, HBeAb, and total HBcAb were assessed by immune-enzymatic assays. The quantification of HBV DNA was performed by real-time PCR. RESULTS: The overall prevalence of positive cases for HBsAg, HBsAb, and total HBcAb was 2.47%, 27.66%, and 21.2%, respectively. From 141 patients with an isolated HBcAb serological profile (HBcAb+/HBsAb-/HBsAg-), HBV DNA was detected in 10 patients, representing a rate of 7.09%. In the present study, up to 95.78% of HBV chronic carriers were negative for HBeAg. CONCLUSION: This study highlights a higher prevalence of HBsAg in the hospital-based population than the general population reported previously in Morocco and a very low HBV immunization coverage. Of particular interest, detectable HBV DNA levels in isolated HBcAb patients show that exclusive HBsAg screening cannot eliminate the risk of HBV transmission in certain cases. Many efforts are then mandatory to promote serological testing and increase the vaccination rate to limit viral dissemination for better management of this disease in Morocco.
Assuntos
Vírus da Hepatite B , Hepatite B , Hepatite B/diagnóstico , Hepatite B/epidemiologia , Anticorpos Anti-Hepatite B , Antígenos de Superfície da Hepatite B , Vírus da Hepatite B/genética , Hospitais Universitários , Humanos , Marrocos/epidemiologia , PrevalênciaRESUMO
The early detection and genotyping of the hepatitis C virus (HCV) are important in the management of this infection. The genotype is the major factor influencing treatment decisions. That's why it is necessary to use fast and accurate methods in its determination. This study reports, over a period of 3 years (from May 2012 to July 2015), the percentage of indeterminate genotypes by the Abbott RealTime HCV Genotype II test and their results using a sequencing technique. Of 309 samples of 309 patients tested, 11 were indeterminate (4.4%). There were three cases of cross-reactivity (1b/4 in one case, 2/5 in two cases) and a possible co-infection 1 + 4. Among those indeterminate genotypes, cross-reactivities and co-infections, ten samples were tested by sequencing. The results were for four of them a 1d subtype, five were a 2i subtype and one was a 2l subtype. These results support the thesis of complementarity between the two methods: genotyping for the detection of mixed reactions and sequencing for resolving indeterminate cases by genotyping.
Assuntos
Genótipo , Técnicas de Genotipagem/métodos , Hepacivirus/classificação , Hepacivirus/genética , Hepatite C/virologia , Técnicas de Diagnóstico Molecular/métodos , Feminino , Hepacivirus/isolamento & purificação , Hepatite C/epidemiologia , Humanos , Masculino , Marrocos/epidemiologiaRESUMO
OBJECTIVES: Scarce and limited epidemiological, clinical and microbiological data are available regarding paediatric respiratory tract infections in the Kingdom of Morocco, a middle-income country in northwestern Africa. The results of hospital-based surveillance aiming at describing the aetiology and epidemiology of respiratory distress among children <5 years of age are presented. METHODS: Children admitted to the Hôpital d'Enfants de Rabat, Morocco, and meeting the World Health Organization clinical criteria for severe pneumonia were recruited over a period of 14 months and were thoroughly investigated to ascertain a definitive diagnosis. RESULTS: In total, 700 children were recruited for the study. Most frequent clinical diagnoses included wheezing-related conditions (bronchitis/asthma, 46%; bronchiolitis, 15%), while typical bacterial pneumonia was infrequent (only 19% of the cases). Invasive bacterial disease detected by classical microbiology or molecular methods was also uncommon, affecting only 3.5% of the patients, and with an overall low detection of pneumococcal or Haemophilus influenzae type b disease. Conversely, coverage of respiratory viral detection in the nasopharynx was almost universal among cases (92%), with the three most frequent viruses detected being rhinovirus (53%), respiratory syncytial virus (18%) and adenovirus (17%). The overall case fatality rate (CFR) among recruited patients with a known outcome was 4.1% (28/690). CONCLUSIONS: In Morocco, the epidemiological profile of paediatric acute respiratory infections is markedly shifted towards wheezing-related diseases and thus resembles that of high-income countries. However, the high associated CFRs found in this study call for an improvement in preventive and clinical management strategies.
Assuntos
Hospitalização/estatística & dados numéricos , Nasofaringe/virologia , Pneumonia/epidemiologia , Pneumonia/etiologia , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/etiologia , Doença Aguda , Adenoviridae/isolamento & purificação , Bronquiolite/epidemiologia , Bronquiolite/virologia , Pré-Escolar , Feminino , Hospitais Universitários , Humanos , Lactente , Masculino , Marrocos/epidemiologia , Vigilância da População , Sons Respiratórios/etiologia , Vírus Sinciciais Respiratórios/isolamento & purificação , Rhinovirus/isolamento & purificação , Índice de Gravidade de DoençaRESUMO
INTRODUCTION: Cyclical pertussis epidemics primarily affect young infants. This study aims to estimate pertussis prevalence during the ongoing 2023 outbreak at our institution, focusing on affected age groups and clinical presentations. MATERIEL AND METHODS: This retrospective study includes patients admitted to Rabat University Hospital Center from 1st January 2021 to 30th June 2023. Symptomatic patients underwent Multiplex Respiratory Panel PCR testing for respiratory infections. The analysis included cases where RT-PCR identified Bordetella spp., with data analysed using SPSS 15.0. RESULTS: Pertussis cases sharply increased from December 2022, constituting 85.4 % of positive samples. Most cases (78.2 %) occurred in infants under 3 months, presenting symptoms such as coughing (94.5 %) and dyspnoea (94.5 %). Pertussis was suspected in 60 % of RT-PCR confirmed cases. B. pertussis DNA was identified in 81.8 % of cases and B. parapertussis DNA in 18.2 % of cases. CONCLUSION: The study exposes a significant pertussis outbreak affecting predominantly young infants.
Assuntos
Bordetella pertussis , Surtos de Doenças , Coqueluche , Humanos , Coqueluche/epidemiologia , Coqueluche/microbiologia , Lactente , Estudos Retrospectivos , Masculino , Feminino , Bordetella pertussis/genética , Bordetella pertussis/isolamento & purificação , Pré-Escolar , Marrocos/epidemiologia , Criança , Hospitalização/estatística & dados numéricos , Recém-Nascido , Prevalência , Adolescente , Bordetella parapertussis/genética , Bordetella parapertussis/isolamento & purificaçãoRESUMO
OBJECTIVES: The aim of the present research is to update data on the seroprevalence of rubella and to identify the associated risk factors among pregnant women in the Rabat region of Morocco in order to take immediate action to monitor the virus. DESIGN: A cross-sectional study. SETTING: The study was conducted at Ibn Sina University Hospital and at referral healthcare centres in the region of Rabat. PARTICIPANTS: A total of 502 pregnant women (mean age 29.7±6.3 years, range 17-44 years) attending the maternity department during 8 months were selected for serological testing. OUTCOME MEASURES: A structured questionnaire was used to obtain sociodemographic, reproductive and clinical characteristics after obtaining written informed consent. Venous blood samples were collected to determine rubella-specific IgG antibodies using an automated chemiluminescent microparticle immunoassay (ARCHITECT i1000SR and i2000SR, Abbott Diagnostics). RESULTS: Antirubella IgG antibodies (≥10 IU/mL) were found in 408 (85.9%) pregnant women examined. The rate of susceptibility to rubella virus infection among pregnant women was found to be 14.1%. These protective rates were found to differ significantly between uneducated pregnant women (80.9%) and those with university-level education (95.5%) (p=0.02). Pregnant women in the 17-24, 25-34 and 35-44 years age groups accounted for 92.5%, 85.2% and 82.8%, respectively (p=0.015). Also, IgG seropositivity status was found to differ significantly between multiparous (83.3%) and primiparous (92.5%) pregnant women (p=0.01). None of the other characteristics was significantly associated with rubella infections. CONCLUSION: Vaccination programmes need to be updated to ensure that campaigns reach their specified goals. Thus, implementing an effective, large-scale screening programme for congenital rubella infection in different regions of Morocco is highly recommended. On the other hand, seronegative pregnant women should be given special preventive care and health education about rubella transmission and congenital rubella syndrome sequelae.
Assuntos
Complicações Infecciosas na Gravidez , Rubéola (Sarampo Alemão) , Feminino , Gravidez , Humanos , Lactente , Pré-Escolar , Gestantes , Complicações Infecciosas na Gravidez/epidemiologia , Estudos Transversais , Estudos Soroepidemiológicos , Marrocos/epidemiologia , Rubéola (Sarampo Alemão)/epidemiologia , Rubéola (Sarampo Alemão)/prevenção & controle , Vírus da Rubéola , Anticorpos Antivirais , Imunoglobulina GRESUMO
SARS-CoV-2 is a major public health problem worldwide. Since its emergence, several diagnostic kits have been developed to ensure rapid patient management. The aim of our study is to check the performance of the new Moroccan SARS-CoV-2 detection kit: MAScIR SARS-CoV-2 M 2.0. The following parameters were studied: repeatability, reproducibility, analytical specificity, analytical sensitivity, and comparison with the GeneFinder™ COVID-19 Plus RealAmp Kit. In addition, an external quality evaluation comprising five specimens was carried out as part of an international program for the external quality evaluation of sublaboratories of the WHO and the Laboratory Office of the National Institute of Hygiene of Morocco. The results of all parameters studied showed an analytical performance that complied with the requirements of the method verification/validation protocol adopted by the Central Laboratory of Virology and met the recommendations of COFRAC (French Accreditation Committee). During the current study, the sequencing of some randomly selected positive samples was performed, among which the carriers of the Alpha variant, the Delta variant, and the Omicron variant were detected. These results allowed us to deduce that this kit was valid for detecting these three variants.
RESUMO
Respiratory viruses are the most involved pathogens in acute respiratory infections. During the COVID-19 pandemic, new elements have been brought to this topic, especially at the diagnostic and therapeutic level. The objective of this work is to describe the epidemiology of respiratory viruses in patients admitted to the Ibn Sina University Hospital of Rabat during a period characterized by the emergence and spread of SARS-CoV-2. We conducted a retrospective study from January 1 to December 31. We included all patients treated for acute respiratory infection and for whom a multiplex respiratory panel PCR was requested. Virus detection was performed using a FilmArray RP 2.1 plus BioFire multiplex respiratory panel. The study population was relatively adults with a mean age of 39 years. The sex ratio M/F was 1.20. The survey revealed a high prevalence of 42.3% of patients hospitalized in the adult intensive care unit whose respiratory distress was the most common reason for hospitalization (58%). The positivity rate was 48.1%. This rate was higher in the pediatric population 83.13% compared to adults 29.7%. Monoinfection was found in 36.4% of cases, and codetection in 11.7% of cases. This survey revealed that a total of 322 viruses were detected, HRV being the most incriminated virus (48.7%), followed by RSV in 13.8% of patients. Considering the five most detected viruses in our study (HRV, RSV, PIV3, ADV, and hMPV), we found that the incidence was significantly higher in the pediatric population. SARS-CoV-2 was detected only in adult's population. In our study, we found that influenza A and B viruses, PIV2, MERS, and all bacteria were not detected by this kit during the study period. Regarding the seasonal distribution, RSV and hMPV showed a significantly high incidence during autumn and summer and SARS-CoV-2 and CoV OC43 showed a high peak during winter. In this study, we found a lack of detection of influenza virus and a shift in the usual winter peak of RSV to the summer, while the detection of ADV and HRV was less affected. This difference in detection could be due on the one hand to the difference in stability between enveloped and nonenveloped viruses and on the other hand to the escape of certain viruses to the different sanitary measures introduced after the declaration of the COVID-19 pandemic. These same measures were effective against enveloped viruses such as RSV and influenza viruses. The emergence of SARS-CoV-2 has modified the epidemiology of other respiratory viruses, either directly by viral interference or indirectly by the preventive measures taken.
RESUMO
INTRODUCTION: The errors in the pre-analytic phase are at the origin of 60 to 85% of the errors in the results proceeded from laboratory. AIM: To evaluate the impact of the pre-analytical phase on the received bacteriology samples received at the medical bacteriology laboratory of Ibn Sina UH in Rabat Morocco in order to propose corrective actions. METHODS: A descriptive and quantitative study of the non-conformities of the pre-analytical phase identified in the central laboratory of medical bacteriology of the Ibn Sina hospital in Rabat over a period of 6 months (from January 01 to July 01, 2020). It concerned samples from various clinical services and care units of Ibn Sina Hospital, as well as external samples. In order to act on these various dysfunctions, we used quality tools such as the Pareto chart and from Ishikawa. RESULTS: We counted 424 cases of pre-analytical NC on 14468 samples received to the Central Laboratory of Bacteriology or 2.93%. These are mainly errors of sample identification and patient identity (59.66%), quality and quantity of samples (25.02%) and problem of conditions of transport and packaging (15.32%). CONCLUSION: Its mastery requires firstly, a close collaboration between the various services, prescribers, samplers and biologists and secondly the respect of each stage of this phase by the hospital staff.
Assuntos
Bacteriologia , Dermatopatias , Hospitais Universitários , Humanos , Marrocos , Fase Pré-AnalíticaRESUMO
Background: RT-PCR is the gold standard for COVID-19 diagnosis, but the lack of standardization of assays, whose diagnostic performance may widely vary, complicates the interpretation of the discrepancies that may be encountered. Study design. We conducted a retrospective study over a ten-month period at the Central Laboratory of Virology of Ibn Sina University Hospital of Rabat. We included nasopharyngeal swabs, positive and negative for SARS-CoV-2 on FilmArray BioFire® Respiratory Panel 2.1 Plus, which were subjected to our laboratory's reference test, MAScIR SARS-CoV-2 M kit 2.0, initially or after a freeze-thaw cycle. The results were compared, and each discrepant sample with sufficient volume underwent the third test, using ARGENE® SARS-CoV-2 R-GENE kit. Results: Of 80 SARS-CoV-2 negative samples on FilmArray, there were no discordant results, whereas of 80 SARS-CoV-2 positive samples on FilmArray, 21 had discordant results on MAScIR, and only 11 could be tested on ARGENE, revealing positive results in 6 cases. 12.7% and 76.5% correspond to the discordance rates for MAScIR (with one or both targets detected on FilmArray), while 14.3% and 100% correspond to those of ARGENE. As the estimated sensitivity and specificity of FilmArray, compared with MAScIR, were 100% and 79.2%, respectively, its lower limit of detection, and ARGENE assay results, made it difficult to distinguish between false positives on FilmArray and false negatives on MAScIR without further investigations. Conclusion: The implementation of a new assay in our laboratory revealed discrepancies suggesting a lack of sensitivity of our laboratory's reference test, leading us consequently to retain the SARS-CoV-2 positive result of these discordant samples on FilmArray, regardless of the detection of one or both targets. Our study, which is, to our knowledge, the first comparing FilmArray RP2.1 and MAScIR 2.0 assays for SARS-CoV-2 detection, highlights the urgent need to standardize RT-PCR assays for COVID-19 diagnosis.
RESUMO
Here, we report the near-complete genome sequence and genetic variations of a clinical sample of SARS-CoV-2 for the newly emerged Omicron variant (BA.1). The sample was collected from a nasopharyngeal swab of a Moroccan patient, and the sequencing was done using Ion S5 technology.
RESUMO
INTRODUCTION: The laboratory of bacteriology is a place with a potential risk and permanent exhibition to biological and infectious hazards caused by the handling of potentially contaminated biological products. AIMS: To evaluate biological and infectious, analyze the circumstances of incidents and accidents and propose a professional risk assessment process (EvRP) to the laboratory with an action plan that can be carried out immediately. METHODS: A descriptive epidemiological cross-sectional study carried out in the medical bacteriology laboratory of the Ibn Sina University Hospital in Rabat over a period of twelve months. It is based on self-questionnaires, observations and interviews with staff. RESULTS: The evaluation showed that the staff of this service is highly exposed. AES constitutes the most important biological risk with 28.6% of the staff but without any declaration. The absence of a referent in health risk management to communicate knowledge of biological and infectious risk to staff. Only 14.3% of staff would be up to date for the vaccination. The absence of a post-exposure biological risk action plan and the design of the tuberculosis laboratory does not comply with risk level 3. CONCLUSION: An action plan and prevention strategies must be implemented and their application is based on numerous national and international regulatory texts.
Assuntos
Bacteriologia , Estudos Transversais , Unidades Hospitalares , Hospitais Universitários , Humanos , LaboratóriosRESUMO
Here, we report the near-complete genome sequence and the genetic variations of a clinical sample of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) harboring the N501Y mutation assigned to the B.1.1.7 lineage. The sample was collected from a nasopharyngeal swab of a female patient from Temara, Morocco, and the sequencing was done using Ion S5 technology.
RESUMO
The objective of the study was to describe the aetiology, epidemiology and clinical characteristics of the principal causes of acute infectious diarrhoea requiring hospitalization among children under 5 years of age in Rabat, Morocco. A prospective study was conducted from March 2011 to March 2012, designed to describe the main pathogens causing diarrhoea in hospitalized children >2 months and less than 5 years of age. Among the 122 children included in the study, enteroaggregative Escherichia coli (EAEC) and rotavirus were the main aetiological causes of diarrhoea detected. Twelve (9.8â%) children were referred to an intensive care unit, while two, presenting infection by EAEC, and EAEC plus Shigella sonnei, developed a haemolytic uraemic syndrome. Additionally, six (4.9â%) deaths occurred, with EAEC being isolated in four of these cases. Diarrhoeagenic E. coli and rotavirus play a significant role as the two main causes of severe diarrhoea, while other pathogens, such as norovirus and parasites, seem to have a minimal contribution. Surveillance and prevention programmes to facilitate early recognition and improved management of potentially life-threatening diarrhoea episodes are needed.
Assuntos
Infecções Bacterianas/epidemiologia , Diarreia/epidemiologia , Diarreia/etiologia , Doenças Parasitárias/epidemiologia , Viroses/epidemiologia , Infecções Bacterianas/microbiologia , Infecções Bacterianas/patologia , Pré-Escolar , Diarreia/patologia , Feminino , Hospitalização , Hospitais Pediátricos , Humanos , Lactente , Masculino , Marrocos/epidemiologia , Doenças Parasitárias/microbiologia , Doenças Parasitárias/patologia , Prevalência , Estudos Prospectivos , Centros de Atenção Terciária , Viroses/patologia , Viroses/virologiaRESUMO
OBJECTIVES: Data on prognostic factors among children with severe pneumonia are scarce in middle-income countries. We investigated prognostic factors for an adverse outcome among children admitted to the Hôpital d'Enfants de Rabat, Morocco with World Health Organization-defined clinically severe pneumonia (CSP). METHODS: Children aged 2-59 months admitted to the hospital and fulfilling the CSP definition were recruited into this 13-month prospective study. A poor prognosis was defined as death, a need for intensive care, or a Respiratory Index of Severity in Children (RISC) score ≥3. Multivariate logistic regression was performed to ascertain independent predictive factors for a poor prognosis. RESULTS: Of the 689 children included in this analysis, 55 (8.0%) required intensive care and 28 died (4.0%). Five hundred and two (72.8%) children were classified as having a good prognosis and 187 (27.2%) as having a poor prognosis. A history of prematurity (odds ratio (OR) 2.50, 95% confidence interval (CI) 1.24-5.04), of fever (OR 2.25, 95% CI 1.32-3.83), living in a house with smokers (OR 1.79, 95% CI 1.18-2.72), impaired consciousness (OR 10.96, 95% CI 2.88-41.73), cyanosis (OR 2.09, 95% CI 1.05-4.15), pallor (OR 2.27, 95% CI 1.34-3.84), having rhonchi on auscultation (OR 2.45, 95% CI 1.58-3.79), and human metapneumovirus infection (OR 2.13, 95% CI 1.13-4.02) were all independent risk factors for an adverse outcome, whereas a history of asthma (OR 0.46, 95% CI 0.25-0.84) was the only independent risk factor for a positive outcome. CONCLUSIONS: The early identification of factors associated with a poor prognosis could improve management strategies and the likelihood of survival of Moroccan children with severe pneumonia.
Assuntos
Pneumonia/diagnóstico , Pré-Escolar , Cuidados Críticos , Feminino , Hospitalização , Hospitais Universitários , Humanos , Lactente , Masculino , Marrocos , Pneumonia/mortalidade , Prognóstico , Estudos Prospectivos , Fatores de RiscoRESUMO
Scarce and limited epidemiological, clinical and microbiological data are available regarding pediatric respiratory tract infections in the Kingdom of Morocco, a middle-income country in Northwestern Africa. Data on antibiotic usage for such infections are also scarce. A good understanding of pre-admission and intra-hospital usage of antibiotics in children with respiratory infections linked with an adequate surveillance of the antibiotic susceptibility from circulating pathogens could help policy makers improve their recommendations on management of respiratory infections. We hereby present data on antibiotic usage prior and during admission and antibiotic susceptibility of major circulating respiratory pathogens in children under five years of age admitted to the Hôpital d'Enfants de Rabat, Morocco, with a diagnosis of clinical severe pneumonia (using World Health Organization (WHO) standardized case definitions) during a period of 14 months (November 2010-December 2011), as part of a larger hospital-based surveillance study designed to understand the etiology and epidemiology of severe pneumonia cases among children.
RESUMO
Isolation of bacteria involved in eye infections and their susceptibility pattern to various antibiotics in vitro eye were studied. Different ocular samples were obtained from 354 patients hospitalized in ophthalmology unit. The samples were inoculated directly onto appropriate culture media. Biochemical tests have been performed and microorganisms were identified according to standard procedure. The in vitro susceptibility of pathogens to antibiotics has been studied as recommended by the CA-SFM; 129 bacteria were isolated with a prevalence rate of 36.4%. The conjunctival swabs were predominant in 70.5% (n=91). In conjunctivitis and keratitis, the main causal agent was coagulase-negative staphylococci followed by alpha hemolytic streptococci. Bacteria from contact lenses were represented mostly by Enterobacteriaceae (50%). In uveitis and cellulite, different bacteria were isolated. The pathogens studied were susceptible to most antibiotics tested except Staphylococcus aureus that showed resistance to penicillin G, Pseudomonas sp expressed an average sensitivity to piperacillin and ticarcillin. The alpha hemolytic streptococci showed very low sensitivity to ciprofloxacin. In conclusion many species could be responsible for eye infections. They were sensitive to most antibiotics tested exceptions.