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1.
Mol Cell ; 34(6): 696-709, 2009 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-19560422

RESUMO

MicroRNAs (miRNAs), approximately 22 nt noncoding RNAs, assemble into RNA-induced silencing complexes (RISCs) and localize to cytoplasmic substructures called P bodies. Dictated by base-pair complementarity between miRNA and a target mRNA, miRNAs specifically repress posttranscriptional expression of several mRNAs. Here we report that HIV-1 mRNA interacts with RISC proteins and that disrupting P body structures enhances viral production and infectivity. In HIV-1-infected human T lymphocytes, we identified a highly abundant miRNA, miR-29a, which specifically targets the HIV-1 3'UTR region. Inhibiting miR-29a enhanced HIV-1 viral production and infectivity, whereas expressing a miR-29 mimic suppressed viral replication. We also found that specific miR-29a-HIV-1 mRNA interactions enhance viral mRNA association with RISC and P body proteins. Thus we provide an example of a single host miRNA regulating HIV-1 production and infectivity. These studies highlight the significance of miRNAs and P bodies in modulating host cell interactions with HIV-1 and possibly other viruses.


Assuntos
HIV-1/patogenicidade , MicroRNAs/fisiologia , RNA Mensageiro/fisiologia , RNA Viral/fisiologia , Complexo de Inativação Induzido por RNA/metabolismo , Sequência de Bases , Sítios de Ligação , Estruturas Citoplasmáticas/fisiologia , RNA Helicases DEAD-box/genética , RNA Helicases DEAD-box/metabolismo , HIV-1/genética , HIV-1/metabolismo , Humanos , MicroRNAs/genética , MicroRNAs/metabolismo , Dados de Sequência Molecular , Proteínas Proto-Oncogênicas/genética , Proteínas Proto-Oncogênicas/metabolismo , RNA Mensageiro/química , RNA Viral/química , Ribonuclease III/antagonistas & inibidores , Linfócitos T/virologia , Replicação Viral
2.
J Virol ; 87(16): 8853-61, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23785196

RESUMO

The SF1 helicase MOV10 is an antiviral factor that is incorporated into human immunodeficiency virus type 1 (HIV-1) virions. We now report that HIV-1 virions also incorporate UPF1, which belongs to the same SF1 helicase subfamily as MOV10 and functions in the nonsense-mediated decay (NMD) pathway. Unlike ectopic MOV10, the overexpression of UPF1 does not impair the infectivity of HIV-1 progeny virions. However, UPF1 becomes a potent inhibitor of HIV-1 progeny virion infectivity when residues required for its helicase activity are mutated. In contrast, equivalent mutations abolish the antiviral activity of MOV10. Importantly, cells depleted of endogenous UPF1, but not of another NMD core component, produce HIV-1 virions of substantially lower specific infectivity. The defect is at the level of reverse transcription, the same stage of the HIV-1 life cycle inhibited by ectopic MOV10. Thus, whereas ectopic MOV10 restricts HIV-1 replication, the related UPF1 helicase functions as a cofactor at an early postentry step.


Assuntos
Interações Hospedeiro-Patógeno , Transativadores/metabolismo , Replicação Viral , HIV-1/fisiologia , Humanos , Proteínas Mutantes/genética , Proteínas Mutantes/metabolismo , RNA Helicases , Transativadores/genética
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