RESUMO
Background and Aim: To achieve optimal feed efficiency in ruminants, especially Pesisir cattle, it is necessary to maintain a harmonious equilibrium between energy and protein levels within the rumen. Sulfur supplementation can potentially escalate the energy-protein balance in the rumen. The aim of this study was to explore the formulation of ruminant diets by synchronizing rumen degradable protein (RDP) and non-fiber carbohydrate (NFC) while adding sulfur minerals at different levels. Nutrient digestibility, NH3 concentration, volatile fatty acids (VFA) production, microbial protein synthesis (MPS), and methane gas production were assessed. Materials and Methods: We employed a randomized block design with a 2 × 2 × 3 factorial arrangement and examined diverse incubation periods of 6, 24, and 48 h. Treatment consisted of RDP (60% and 65%), NFC (35% and 40%), and sulfur (0%, 0.15%, and 0.3%) levels. In this study, the Tilley and Terry in vitro technique, which used Pesisir cattle's rumen fluid, was employed to assess the digestibility of dry matter, organic matter, acid detergent fiber, neutral detergent fiber, and RDP-Rumen undegradable protein. In addition, it measures various rumen fluid attributes, including pH, NH3, VFA, MPS, and methane gas production. Results: Treatment with a coordinated combination of 65% RDP and 40% NFC combined with 0.15% sulfur supplement yielded significantly improved digestibility and notably reduced methane gas production (p < 0.05). Conclusion: The enhancement in digestibility and reduction in methane gas emissions can be attributed to the interaction of RDP, NFC, and sulfur. Feed digestibility was increased in the 65% RDP treatment with 40% NFC and 0.15% sulfur, along with a decrease in methane gas production.