Ecological and human health risk assessment of total petroleum hydrocarbons in surface water and sediment from Woji Creek in the Niger Delta Estuary of Rivers State, Nigeria.

This study was designed to assess total petroleum hydrocarbon (THP) concentrations in the surface water and sediment sampled from Woji Creek and to assess potential ecological and human health risk due to petroleum hydrocarbons along the creek. Physicochemical parameters [pH, temperature (T), electrical conductivity (EC), dissolved oxygen (DO), total dissolved solids (TDS)] were in-situ measured from sediment and surface water; hydrological parameters (width, depth and volume) were used to calculate the flow rate (discharge) at different stations of the creek. Trend of TPH in the surface water samples along the creek were as follows: St4 (3.639 ± 1.121 mg/L) > St3 (2.449 ± 0.623 mg/L) > St1 (1.457 ± 0.244 mg/L) > St2 (1.069 ± 0.228 mg/L) > St5 (1.010 ± 0.120 mg/L) Trend of TPH concentration across the creek was as follows: St1 - 8.758 ± 0.697 mg/kg > St3 - 7.675 ± 0.541 mg/kg > St5 - 5.515 ± 0.401 mg/kg > St4 - 5.075 ± 0.363 mg/kg > St2 - 3.162 ± 0.307 mg/kg. Diagnostic indices indicate that the hydrocarbon in the creek was from petrogenic source. Estimation of ecological risk indicated risk in the surface water but not in the sediment. However, human health risk assessment indicated no risk due to human ingestion of the sediment or surface water.

In situ assessment of Karaj River genotoxic impact with the alkaline comet assay and micronucleus test, on feral brown trout (Salmo trutta fario).

The in situ evaluation of the genotoxic impact of the Karaj River was performed using the comet and micronucleus (MN) assays in erythrocytes, liver, gill and kidney of indigenous brown trout, Salmo trutta fario from three different stations, including Varangerud, Asara and Purkan. The results showed that DNA damage significantly increased in sampled fish erythrocytes, liver and gill from low levels in the upstream river (Varangerud) via intermediate levels in downstream (Purkan) to high levels in the middle of the river (Asara), correlating with the river increasing pollution gradient. Gill was the most sensitive tissue followed by blood and liver. Kidney did not respond to the genotoxic gradient of the river. MN test (as a complementary assay) of liver cells of fish was a sensitive biomarker of genotoxic exposure. MN test in blood, gill and kidney did not reflect the genotoxic condition of the river.