[Redistribution of Ca2+ ions in lymphocyte suspensions after treatment with an ionophore]. / Pereraspredelenie ionov Ca2+ v suspenzii limfotsitov pod deistviem ionofora.

Ionophore A23187 induces three-phase kinetics of reduction of chlorotetracycline (CTC) lauded lymphocyte fluorescence: fast reduction, low increase and lower reduction of fluorescence. We suggested the following explanation of kinetics. The first phase is extinguishing of the Ca2+ - CTC complex fluorescence induced by ionophore A23187 perturbation of the cell membrane, the second one is the energy dependent Ca2+ entry into the cell and the third one is the slow efflux of Ca2+ ions from the cell to concentration gradient.

[Mechanism of activating the lymphocyte glucocorticoid receptor in acute myocardial infarct]. / O mekhanizme aktivatsii gliukokortikoid-retseptornogo apparata limfotsitov pri ostrom infarkte miokarda.

Dose-dependent increase in content of highly specific binding sites for glucocorticoids (receptors), but without alteration in their affinity to the hormone, was observed after incubation during 2 hrs of healthy donor lymphocytes with blood serum of patients with acute myocardial infarction. The similar effects exhibited protein extracts of necrotized and normal parts of human myocardium (heart antigens) as well as the autologous blood serum and human blood serum albumin treated with UV-irradiation. Number of receptors was not altered in human skin fibroblasts incubated both with the patients blood serum and with the UV-treated blood serum. Antigenic effects of protein components, developed in blood serum after acute myocardial infarction, on cells of the lymphoid system appear to be responsible for the increase in number of binding sites for glucocorticoids in lymphocytes of patients with acute myocardial infarction or in lymphocytes of healthy donors incubated with the patients blood serum.

[The amount of thrombocyte alpha 2-adrenoreceptors in patients with different clinical variants in the course of ischemic heart disease]. / Kolichestvo al'fa 2-adrenoretseptorov trombotsitov u bol'nykh s razlichnymi klinicheskimi variantami techeniia ishemicheskoi bolezni serdtsa.

Number of alpha-2-adrenoceptors on the thrombocyte membranes was measured with selective antagonists [H]3 yohimbine in 25 patients with ischemic heart disease (7 patients with unstable 12 with vasospastic and 6 with new onset angina pectoris) and in 16 patients without ischemic heart disease and coronary atherosclerosis (control group). The number of alpha-2-adrenoceptors in patients with unstable angina pectoris (571.0 +/- 92.9) was proved to exceed significantly that in patients with vasospastic (237.9 +/- 30.0) or new onset angina (126.2 +/- 19.3) and in the control group (200.0 +/- 22.5). The number of adrenoceptors tended to decrease during myocardial ischemia.

[Changes in the characteristics of glucocorticoid hormone binding in human peripheral blood lymphocytes during myocardial infarct]. / Izmeneniia kharakteristik sviazyvaniia gliukokortikoidnykh gormonov v limfotsitakh perifericheskoi krovi cheloveka pri infarkte miokarda.

Competitive binding was used to determine the number of binding sites to glucocorticoid hormones in peripheral blood leukocytes of normal subjects, patients with unstable angina pectoris and patients with myocardial infarction. A sharp (2-3-fold) increase in the number of binding sites was observed in patients with myocardial infarction during the first 24 hours after the onset of the disease.