RESUMO
Comparative histopathological study and analysis of parasite load in different muscle groups were carried out in BALB/c mice during the acute phase of Chagas disease. Activities of C104 clone of T. cruzi strain TPAP/MX/2002/Albarrada and the parental strain were compared. Panoramic 2D-microscopy imaging of sample surface was used and quantitative analysis of parasitism and pathologic damage was performed. The infection rates in various muscle groups were as follows: myocardium=abdominal muscles=lumbar muscles=femoral muscles<--diaphragm for the clone and myocardium¬abdominal muscles=lumbar muscles=femoral muscles-->diaphragm for the parental strain.
Assuntos
Doença de Chagas/parasitologia , Trypanosoma cruzi/patogenicidade , Músculos Abdominais/parasitologia , Animais , Diafragma/parasitologia , Coração/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Miosite/parasitologia , Especificidade de Órgãos , Carga Parasitária , Trypanosoma cruzi/genéticaRESUMO
Vacuolizing toxin (VacA) Helicobacter pylori is an important factor of pathogenicity of Helicobacter pylori and a basic marker in the diagnosis of helicobacteriosis and related diseases. A coagulation-based diagnostic test-system was elaborated for the detection of VacA in clinical samples. A fragment of vacA was cloned, for the purpose, in Escherichia coli and expressed in preparative quantities; the coded protein was purified and used in raising the diagnostic serum. The thus designed coagulating test-system was successfully tested under the modeling conditions with clinical samples. Therefore, the designed express method can be used for the invasion-free determination of VacA in patients with gastric and duodenal pathologies.
Assuntos
Testes de Aglutinação/métodos , Proteínas de Bactérias/genética , Infecções por Helicobacter/diagnóstico , Helicobacter pylori/isolamento & purificação , Animais , Anticorpos Antibacterianos/imunologia , Proteínas de Bactérias/análise , Proteínas de Bactérias/sangue , Clonagem Molecular , Escherichia coli/genética , Escherichia coli/metabolismo , Fezes/microbiologia , Infecções por Helicobacter/sangue , Helicobacter pylori/imunologia , Helicobacter pylori/patogenicidade , Humanos , Soros Imunes/imunologia , Coelhos , Saliva/microbiologia , Sensibilidade e EspecificidadeRESUMO
The experiments with passive transfer of splenocytes obtained from animals immunized with Trypanosoma cruzi lysate revealed the role of cell-mediated component of the immunity in the antitumor effect of T. cruzi. The common features of T. cruzi antigens and tumor-specific antigens of Ehrlich's adenocarcinoma were demonstrated. These antigens were shown to have common epitopes with mammalian mucins. The oncoprotective effect was achieved by immunization with type II and III mucins and was reproduced after passive transfer of splenocytes from immunized animals.
Assuntos
Anticorpos Antiprotozoários/imunologia , Antineoplásicos/farmacologia , Carcinoma de Ehrlich/prevenção & controle , Trypanosoma cruzi/imunologia , Animais , Camundongos , Camundongos Endogâmicos BALB C , Mucina-2/imunologia , Mucina-3/imunologia , Transplante de NeoplasiasRESUMO
The direct inhibitory effect of Trypanosoma cruzi epimastigote lysates on in vitro cultured human breast cancer MCF-7 cells differs in various genetic groups and cloned subgroups of Trypanosoma.