Gene sequencing applications to combat oral-cavity related disorders: a systematic review with meta-analysis.

Gene sequencing (GS) has numerous applications in combatting oral-cavity related disorders, including identifying genetic risk factors for diseases, developing targeted therapies, and improving diagnostic methods. It can help identify specific genetic mutations or variations that increase the risk of developing oral-cavity related disorders, such as oral cancer, periodontal disease, and cleft lip and palate. By the means of the following investigation, our primary objective was to assess the impact of GS technique in diagnosing and potentially treating diseases of the oral cavity by the means of a systematic review and meta-analysis. We commenced by defining the terms "gene sequencing," "oral cavity," and "disorders" as the important elements in our investigation's subject. Next, relevant databases like PubMed, Scopus, Embase, Web of Science, and Google Scholar were searched using keywords and synonyms for each concept, such as "genomic sequencing," "DNA sequencing," "oral health," "oral diseases," "dental caries," "periodontal disease," "oral cancer," and "salivary gland disorders." We combined several search terms, such as "gene sequencing AND oral disorders AND periodontal disease" or "oral cancer OR genomic sequencing," to further hone your search results using Boolean operators like "AND" and "OR." The oral cavity analysis obtained by CS in the selected articles revealed that most of the disorders were, in fact, a direct causal event influenced by the oral microbiome. Moreover, each sampled oral cavity evidenced a different microbial community, which predicted the precipitation of benign as well as malignant conditions, though not on a definitive basis. In the last ten years, genomic sequencing had advanced remarkably as majority of our selected studies observed, making it possible to diagnose and treat a variety of oral and maxillofacial disorders, including cancer. It was also used to ascertain a person's genetic make-up as well as to spot numerous genetic abnormalities that can predispose individuals to diseases. Understanding the different sequencing techniques and the resulting genetic anomalies may help with their clinical application and lead to an improvement in illness diagnosis and prognosis as a whole in the field of dentistry.

Telemedicine in the diagnosis and management of temporomandibular disorders: A systematic review conducted according to PRISMA guidelines and the Cochrane Handbook for Systematic Reviews of Interventions.

BACKGROUND: Telemedicine (T-Med) has always been an important tool in the arsenal of clinicians worldwide. This technique has become increasingly popular in recent years, especially in light of the COVID-19 pandemic, which has made it difficult for some people to access traditional dental care. The current review aimed to analyse the usage of telemedicine in the diagnosis and management of temporomandibular disorders (TMDs) and its impact on general health. METHODS: An extensive search of databases was conducted using keywords such as, "telemedicine," "teledentistry," "TMJ" and "temporomandibular disorders," resulting in a total of 482 papers to be available from which eligible studies were selected. The Risk of Bias in Observational Studies of Exposures (ROBINS-E) tool was used to evaluate methodological quality of included studies. RESULTS: Two studies were selected which fulfilled the eligibility criteria. All assessed studies indicated varying degrees of positive outcomes for patients who were intervened for TMDs using T-Med. CONCLUSION: T-Med shows promising results for the diagnosis and management of TMDs, especially since the advent of the COVID-19 pandemic and thereafter. Long-term clinical trials with larger samples are needed to further ascertain validity in this regard.

Assessment of Symptoms Associated with Temporomandibular Dysfunction and Bruxism among Elderly Population: An Epidemiological Survey.

AIM: The aim of the study was to assess the symptoms associated with temporomandibular disorders (TMD) and bruxism among elderly population in Ranchi, India. MATERIALS AND METHODS: A cross-sectional study was done on 600 elderly subjects; data regarding the signs and symptoms associated with temporomandibular disorder and bruxism were recorded using both structured questionnaire and clinical examination. Spearman correlation analysis was done to find the association between TMD and bruxism. RESULTS: Out of 600 subjects, 49% were males and 51% were females. The overall prevalence of TMD-related symptoms like temporomandibular joint (TMJ) pain, difficulty in jaw opening, TMJ sounds, and bruxism were 10.5, 11.2, 14, and 17% among elderly subjects. TMD symptoms and bruxism were relatively more commonly seen among females when compared to males. According to logistic regression (significantly correlated independent variables, i.e., TMD symptoms among analyzed variables), the dependent variable like bruxism had highest odds ratio, i.e., 8 for 60-70 years age-group and 15.1 for 70-80 year age-group. CONCLUSION: There was a lesser prevalence of symptoms related to TMD and bruxism among the study population, and bruxism had the highest odds ratio in TMD between the analyzed variables. CLINICAL SIGNIFICANCE: Human aging contributes too many oral problems, while resolving these, the felt needs of the population are sometimes ignored which adds up to the growing list of issues. Studies have shown inconclusive evidence regarding the prevalence of symptoms related to TMD and bruxism as these are known to trouble elderly populations.

Clinical Success of Screw-retained Dental Implants: A Systematic Review.

OBJECTIVE: The objective of the research was to review the literature on clinical evaluation and success of screw-retained dental implants by assessing the marginal bone loss (MBL). METHODS: Online electronic databases such as PubMed/MEDLINE, Google Scholar, and Cochrane Library were searched using appropriate keywords for the last 20 years, dated from January 1, 2000, till August 1, 2021, with a restriction on language. Additional sources like major journals, unpublished studies, conference proceedings, and cross-references were explored. Information curated for data extraction included methodology, population, type of implants used, and duration of follow-up. RESULTS: The PubMed/MEDLINE, Google Scholar, Cochrane Library, and additional sources identified a huge number, out of which 637 search results were screened, out of which 322 were duplicates. The remaining 315 unique studies were screened for the titles and abstracts, and 23 articles were selected for full-text screening. A total of six articles that matched the eligibility criteria were processed for qualitative analysis. CONCLUSION: Despite the uncertain retrievability of screw-retained implant-supported fixed restorations, this treatment option in fixed implant prosthodontics is a reliable and effective choice, especially for implant-supported long-span fixed partial dentures (FPDs), full-arch FPDs, and cantilever FPDs.

Biocompatibility of 3D-Printed Dental Resins: A Systematic Review.

BACKGROUND: The biocompatibility of 3D-printed dental resins has become a critical concern in modern dentistry due to the increasing utilization of additive manufacturing (AM) techniques in dental applications. These resins serve as essential materials for fabricating dental prostheses, orthodontic devices, and various dental components. As the clinical adoption of 3D printing in dentistry grows, it is imperative to comprehensively assess the biocompatibility of these materials to ensure patient safety and dental treatment efficacy. This systematic review aimed to evaluate the existing body of literature on the biocompatibility of 3D-printed dental resins, thereby providing valuable insights into the potential biological risks associated with their use. METHODS: The search strategy to identify relevant papers was implemented across PubMed/MEDLINE, Scopus, Web of Science, Embase, Cochrane Library, CINAHL, and Google Scholar to identify relevant studies. Study selection was not limited to any particular timeframe of publishing. The revised CONSORT criteria were used to ascertain the authenticity and dependability of the review's outcomes. Comprehensive screening and eligibility assessment processes were conducted to select studies meeting predefined criteria. Biocompatibility-related parameters, including toxicity, mechanical properties, cell viability, and other relevant outcomes, were analyzed across selected studies using a standardized variable extraction protocol. RESULTS: A total of 9 studies were included in the systematic review. The findings encompassed various aspects of biocompatibility assessment, including material composition, mechanical properties, cell viability, and cytotoxicity. Some studies revealed significant improvements in flexural strength and cell viability with specific resin formulations, demonstrating their potential for enhanced clinical utility. Conversely, certain resins exhibited cytotoxicity, while others displayed promising biocompatibility profiles. CONCLUSION: As per the assessed findings, material composition, post-processing techniques, and manufacturing methods emerged as critical factors influencing biocompatibility outcomes. While some resins exhibited favorable biocompatibility profiles, others raised concerns due to cytotoxicity. These findings emphasize the need for careful consideration when selecting and implementing 3D-printed dental resins, with a focus on materials engineering and comprehensive biocompatibility testing. Further research is warranted to elucidate the long-term biocompatibility and clinical implications of these materials.

Immuno-histochemical evaluation of CD34 for OLP and OSMF.

Immuno-histochemical evaluation of CD34 in oral lichen planus (OLP) and Oral Submucous Fibrosis (OSMF) is of interest to dentist.20 specimens of normal oral mucosa (buccal mucosa/gingiva tissue) from patients who had extractions performed as part of orthodontic treatment comprised Group I, the control group. Group II comprised 30 individuals with a diagnosis of oral lichen planus. 30 OSMF cases with diagnoses is Group III. These 80 specimens were all given consideration when choosing for CD34immuno-histochemical staining. The CD34 was greater in all categories of OLP and OSMF when compared to normal controls. Maximum CD34 expression was observed in erosive OLP (147.41±17.60) followed by OSMF (116.01 ±11.72) and reticular OLP (105.01±11.62). Data was statistically significant (p<0.001).Immunohistochemistry of CD34 evaluation is a potential diagnostic marker for OLP and OSMF.

Primary mucosal melanoma: report of two cases.

Primary mucosal malignant melanoma is of unusual occurrence and poor prognosis. We report two cases of primary mucosal melanoma, one in a 70-year-old male in the palate and the other in a 73-year-old female in the maxillary sinus. Early diagnosis and proper clinical and cytologic evaluation is necessary to institute therapeutic intervention. These cases are reported on account of their rarity of occurrence and difficulty of treatment.

Clinical and radiographic peri-implant outcomes with riboflavin loaded Poly-L-glycolic acid nanoparticles incorporated in aloe-vera gel treating peri-implantitis in chronic hyperglycemic patients.

AIM: The objective of the current study was to compare the effectiveness of antimicrobial photodynamic therapy (PDT) versus Poly-L-glycolic acid nanoparticles loaded riboflavin incorporated in aloe vera gel (PGA/RF/AV) on periimplant parameters and bacterial counts in chronic hyperglycemic patients having periimplantitis. METHODS: One hundred and two diabetic patients undergoing mechanical debridement (MD) were equally divided into three groups: Group 1: PGA/RF/AV+ MD, Group 2: PDT + MD, and Group 3: MD alone. Periimplant parameters [Bleeding on probing (BoP), probing depth (PD), plaque index (PI), marginal bone level (MBL)] were recorded in all three groups. Periimplant plaque samples were studied to record the levels of Tannerella forsythia (Tf) and Porphyromonas gingivalis (Pg). The recordings were taken at baseline, 3 months and 6 months post treatment. RESULTS: All three groups showed a reduction in severity of periimplantitis measured in terms of PD, PI, MBL and BoP. A statistically significant reduction in PD, PI and MBL was found in patients in Group 2 whereas participants of Group 1 were found to have a significant decrease in BoP. A statistically significant decline in the numbers of both the bacterial species was seen in Group 2 at the three-month follow-up whereas at the six-month follow-up, a statistically significant reduction was observed in treatment Group 2 in the levels of Tf species only. CONCLUSION: Riboflavin-loaded nanocarrier incorporated in aloe vera gel showed greater clinical efficacy than PDT alone in the treatment of periimplantitis in chronic hyperglycemic individuals.

Micro Tensile bond strength and microleakage assessment of total-etch and self-etch adhesive bonded to carious affected dentin disinfected with Chlorhexidine, Curcumin, and Malachite green.

AIMS AND OBJECTIVES: Assessment of micro tensile bond strength (microTBS) and micro-leakage scores of total-etch adhesive (TAE) and self-etch adhesive (SAE) bonded to carious affected dentin (CAD) sterilized using different cavity disinfectant (curcumin photosensitizer (CP) and malachite green (MG) and Chlorhexidine (CHX) in comparison to no disinfection (ND) control group. MATERIALS AND METHODS: One hundred and twenty human molars having International Caries Detection and Assessment System (ICDAS) scores of 4 and 5 were included. Visual inspection, dental explorer hardness testing, and caries detector solution using 5% basic Fuchsin dye solution was applied to dentin to identify the CAD surface. All the specimens were divided into four groups (n = 30) according to the cavity disinfectants used. Group A: 2% CHX, Group B: CP, Group C: MG, and Group D: ND. Each group was further divided into two subgroups (n = 15) based on the adhesion protocol. Groups A1, B1, C1, and D1 were held using TEA, and groups A2, B2, C2, and D2 were adhered using SEA system. The composite material was then built in 2 mm increments and then cured with light. MicroTBS and failure mode assessment using a universal testing machine (UTM) and stereomicroscope at a 40X magnification was performed on 10 samples from each subgroup. The microleakage assessment was performed using a dye penetration test on five samples from each group. ANOVA and Tukey post-hoc tests were used to compare means and standard deviation (SD) of bond strength and microleakage (p < 0.05). RESULTS The maximum microTBS was displayed by A1= CHX and TEA (13.28± 1.01 MPa). The lowest bond scores were demonstrated by C2= MG and SEA (5.98±0.44 MPa). The highest micro-leakage was exhibited by C1= MG and TEA (58.32 ± 2.11 nm). Whereas, the lowest micro-leakage values were displayed by A2= CHX and SEA (24.34± 1.11 nm). CONCLUSION: Chlorohexidiene displayed better bond strength and the lowest microleakage scores with Total-etch adhesive and Self-etch adhesives when used as a cavity disinfectant. Total-etch adhesives performed better in terms of microTBS scores whereas self-etch adhesives displayed superior seal ability within the same disinfectant group.

Papillon-Lefévre Syndrome: A Rare Case Report and a Brief Review of Literature.

BACKGROUND: Palmar-plantar hyperkeratosis and severe early-onset periodontitis are the hallmarks of the uncommon autosomal recessive Papillon-Lefévre syndrome (PLS), which may cause both primary and permanent teeth to be lost at an early age. The cause and pathophysiology of the disorder involve several factors, including genetic, immunological, and microbial factors. AIM: The purpose of this case study is to provide insight into the fascinating role of consanguinity in the aetiology of this unusual illness. CASE PRESENTATION: An unusual PLS case report in a household with two consanguineously married parents was provided. A 17-year-old Saudi boy visited the dental clinic at Riyadh Elm University because he was having problems with loose teeth and pain while chewing, as well as irritated and friable gums. He may be suffering from a genetic condition that has been effectively treated in the past by his elder brother, who is now 26 years old. In this instance, severe extensive periodontitis contributed to the early loss of primary teeth as well as permanent teeth, resulting in PLS. On the lateral surface of the soles, the distinctive skin lesions revealed hyperkeratosis with regions of persistent thickening, flaking, and scaling. There were erythematous patches on the palms, but no hyperkeratosis was seen. CONCLUSION: When it comes to Papillon-Lefévre syndrome (PLS), this is an extremely unusual instance since two siblings in the same family were both afflicted. Patients who are stigmatised because of their condition will benefit from early discovery and multidisciplinary treatment.

Evaluation of the Histological Changes in the Structure of the Minor Salivary Glands in Patients With Oral Submucous Fibrosis (OSMF).

BACKGROUND: The minor salivary glands (MSGs) are critical components of the mouth's delicate environment. The pre-malignant changes of oral submucous fibrosis (OSMF) have been associated with a decline in the quality of life and an uptick in the prevalence of oral malignancies. AIM: The aim was to provide evidence of the histological alterations in minor salivary gland structure seen in individuals with OSMF. METHODS AND MATERIALS: A total of 106 confirmed cases of OSMF were enrolled in the study. In order to perform an incisional biopsy, we first collected the patient's complete demographic and clinical history. Using a Vernier calliper, the inter-incisal distance was used to evaluate the mouth opening of the patients. An incisional biopsy of the buccal mucosa was carried out using a 6 mm diameter punch and local anaesthesia. After the appropriate demographic and medical information had been gathered. Acinar cells and the surrounding stroma of tissue slices were observed under a light microscope for alterations. The cytoplasm, nucleus morphology, cellular shape, mucin pooling, and acinar outline of acinar cells were all examined by the researchers. It was taken into account to classify OSMF histologically based on variations in juxta epithelial hyalinization. RESULTS: Multiple aetiologies for the symptoms of OSMF were reflected in the patient's histological abnormalities in the minor salivary glands. On the measurement of the diameter of acini, we discover that the average area of salivary gland acini in OSMF patients is smaller than in the normal group indicative of a decrease in size. The number of functional acini in OSMF is fewer than compared in the control group. CONCLUSION:  Because of the findings of this study, we now have a better understanding of the factors that play a role in the incidence of dryness of the oral and pharyngeal mucosa (OSMF), although it has to be mentioned that no major impact of OSMF on minor salivary glands was observed in our study.

Traumatic Bone Cyst of the Anterior Mandibular Region: A Case Report.

A traumatic bone cyst (TBC) is an unusual non-neoplastic pseudocystic cavity in the bone that is often asymptomatic and slow-growing. It is unexpectedly detected by regular radiography imaging. These lesions are more common in the mandible than they are in the maxilla, and they are often seen in patients older than 40 years of age. A radiolucent unilocular lesion with scalloped margins is the most common radiographic appearance. If the hollow is found to contain blood or straw-colored fluid, surgical exploration is the only way to make a conclusive diagnosis of this uncommon condition. We present a case of an asymptomatic, incidentally diagnosed (on radiograph) traumatic bone cyst in a young patient involving the mandibular anterior region with periapical radiolucency. The case was diagnosed by radiographs and histopathological evaluation.

Impact of covid-19 on education, psychological wellness and life style of dental students in Saudi Arabia.

The COVID-19 epidemic has had a significant impact on dental education, psychological health, and students' way of life worldwide. The new methods of teaching and learning had to be adapted by dental educational institutions. Due to lifestyle changes, COVID-19 had a major negative influence on students' mental health. Therefore, it is of interest to assess depression; anxiety and stress scale (DASS-21) levels experienced by dental students and compare them with gender and course of students. A cross-sectional survey based descriptive study was conducted among 356 undergraduate dental students, aged between 18-31 years, at Riyadh Elm University, Riyadh, kingdom of Saudi Arabia from January - March, 2022. A self-administered structured questionnaire written in English and Arabic language was given to all the willing participants. Four components of the questionnaire covered demographic information, the effect of COVID-19 on dental education, psychological health, and student lifestyle. In order to establish statistically significant variations across genders and student course levels regarding the effect of COVID-19 on dentistry education, the Chi-square test was used. Mann-Whitney U test was used to compare depression, anxiety, stress scales (DASS- 21) with variables such as gender and course level of students. Statistical significance was set at p-value <0.05.A total of 356 dental students were involved in this study with the total response rate of 92.2%.Majority of the respondents were males (56.2%) than females 156 (43.8), aged between 18-22 years (53.4%). Overall, moderate levels of the depression, anxiety and stress scores (DASS-21) were seen among 46.9%, 30.3% and 35.6% of the dental students, respectively. Thus, it can be concluded that COVID-19 pandemic had a profound impact on dentistry students' education, mental health, and way of life. More women and dentistry students on the clinical level felt the effects on their education. In order to implement psychological empowerment initiatives integrating institutional counseling services for students, the right steps should be done.

A review on salivary constituents and their role in diagnostics.

Salivary secretions possess a number of biomarkers beneficial for the diagnosis of a plethora of oral and other systemic disorders. Therefore, it is of interest to analyse and discuss the significance of saliva and its constituents as a valuable tool in aiding diagnostics in clinical settings by reviewing available literature and controlled trials. Using the PRISMA framework, a thorough review of research that were listed in the PubMed, Web of Science, and Cochrane library databases was performed which revealed 212 papers, 54 of which were thoroughly evaluated. Exclusion and inclusion criteria were used to choose studies that were applicable for the review. The selected studies reported a number of diseases that were diagnosed using salivary constituents in the same way as other methods of diagnosis, with a number of benefits. Thus, data shows that saliva is an excellent source for biomarkers which can be used for furthering medical diagnosis like other diagnostic procedures.

Collagen Structure, Synthesis, and Its Applications: A Systematic Review.

Resorbable collagen has been utilized to treat wounds, close graft, and tooth extraction sites, and enhance recovery. Collagen-based membranes are also used as barriers in periodontal and implant therapy to limit epithelial migration and allow cells with the regenerative capacity to fill the problem area. This systematic review was carried out to analyze the studies focusing on collagen structure, synthesis, and its applications. A detailed and extensive search was performed with the help of the keywords "collagen structure", "collagen synthesis" and "collagen applications". There was extensive literature search in reliable and authentic databases like PubMed, Scopus, Web of Sciences, Ovidsp, and Cochrane library to obtain papers focusing on collagen structure, synthesis, and applications. During the systematic review, data were obtained concerning the following parameters. Type of study, nature of aim of the study, size of the sample in the study, gender and age of the subjects included in the study, prevalence of skin diseases where collagen was used for treatment, dose of collagen used, form in which collagen was used, the origin of collagen used, analysis of different variables, structure, and synthesis of collagen. Twenty-two studies were included in this systematic review. The studies discussed the structure, synthesis, and applications of collagen in treatment. In studies focusing on the application of collagen supplements, most of the study subjects were females (68.3%). The study subjects included both healthy and unhealthy subjects. The study subjects were divided into two categories. One category was the intervention group, while another group was the placebo group. Collagen was administered in hydrolysate form (90%) in some studies, bovine form (2.3%), and porcine form (3.4%) in other studies. Collagen supplementation was found to provide better results in both healthy and unhealthy effects in improving the health of skin, cornea, bone, periodontium, face, etc. It can be concluded that collagen is an integral part of the body. The application of collagen supplements can be pretty effective in maintaining the proper health of several important structures of the body like skin, face, cornea, nails, periodontium, etc. Thus, a detailed study of the molecular structure of collagen and genes associated with each type of collagen is essential for further research and treatment of collagen-associated disorders.

Noncarious cervical lesion pretreated using antimicrobial photodynamic therapy and diode laser in reducing dentin hypersensitivity bonded to different restorative material: Valuation of bond values and invitro dye leakage.

AIM: The present study aimed to appraise the effect of Diode laser (DL), riboflavin, and curcumin on the bond strength of adhesive restorative material and the effects of pretreatment on microleakage scores. MATERIAL AND METHODS: Ninety caries-free human permanent premolars were obtained and disinfected. All specimens were mounted till CEJ. To provide standardization, a 2 × 2 mm flat non-carious cervical lesion (NCCL) dentin surface was exposed. This was followed by dentin surface roughening/cervical groove preparation with a round bur. After tooth surface preparation, ninety specimens were divided arbitrarily into three groups. Group 1: NCCL exposed to DL; group 2: NCCL pretreated with riboflavin photosensitizer (RFP) and group 3 pretreated with curcumin photosensitizer (CP). All the three groups were further divided into two subgroups A1, A2, B1, B2, C1, and C2 based on the restorative material used (n = 15). Subgroup A1, B1, and C1 were restored with composite resin (CR). Subgroup A2, B2, and C2 were restored with RMGIC. Ten samples from each subgroup were evaluated for shear bond strength (SBS) testing under a universal testing machine (UTM). Five from each subgroup were evaluated for microleakage assessment. Bond strength and microleakage analysis were performed using analysis of variance (ANOVA) with a Post-hoc test (Tukey's post hoc test) at a significance level (p = 0.05). RESULTS: The group A1, DL-CR shows the highest SBS (16.41± 0.4 MPa). However, CP-RMGIC groups demonstrated the lowest bond integrity. RFP-CR and the DL-CR group displayed comparable outcomes of SBS (p>0.05). The highest microleakage was observed with CP-RMGIC. The least microleakage was displayed by DL-CR. CONCLUSION: Noncarious cervical lesions pretreated with diode laser and riboflavin photosensitizer showed better shear bond strength and reduced microleakage when bonded to composite resin than non-carious cervical lesions pretreated with curcumin.