B cell activating factor in obesity is regulated by oxidative stress in adipocytes.

Adipose tissue functions as a key endocrine organ by releasing multiple bioactive substances, and plays a key role in the integration of systemic metabolism. We have previously shown that B cell activating factor is produced mainly in visceral adipose tissue and affects insulin sensitivity in obese individuals. In this study, we identified the signals that lead to production of B cell activating factor in adipocytes. 3T3-L1 and C3H/10T 1/2-clone 8 cells showed increased B cell activating factor expression upon exposure to hydrogen peroxide, and these changes were inhibited by treatment with the antioxidant N-acetyl-cysteine. B cell activating factor levels in both serum and visceral adipose tissue were increased in high fat diet-fed mice, and these increases were correlated with oxidative stress. In addition, serum BAFF levels in high fat diet-fed mice were reduced by N-acetyl-cysteine treatment. We also found that oxidative stress-induced B cell activating factor expression in adipocytes was regulated by NF-κB activation. These data indicate that control of the redox state in visceral adipose tissue is a potentially useful target for treating metabolic syndromes through regulation of adipokine production.

Consensus recommendations for managing osteoarthritic pain with topical NSAIDs in Asia-Pacific.

Osteoarthritis prevalence is expected to increase markedly in the Asia-Pacific region due to rapid population aging. Identifying effective and safe therapeutic options to manage osteoarthritic pain is viewed as a priority. The Asia-Pacific Experts on Topical Analgesics Advisory Board developed consensus statements for use of topical NSAIDs in musculoskeletal pain. Evidence supporting these statements in osteoarthritic pain was reviewed. Best available evidence indicates that topical NSAIDs have a moderate effect on relief of osteoarthritic pain, comparable to that of oral NSAIDs but with a better risk-to-benefit ratio. International clinical practice guidelines recommend topical NSAIDs on par with or ahead of oral NSAIDs for pain management in patients with knee and hand osteoarthritis, and as the first-line choice in persons aged ≥75 years.

Expression pattern of Toll-like receptors (TLRs) in different organs and effects of lipopolysaccharide on the expression of TLR 2 and 4 in reproductive organs of female rabbit.

Toll-like receptors (TLRs) are vital for innate immunity, and they were expressed in various immune cells, tissues and organs. Moreover, TLRs specific expression pattern in different cells, tissue and organs have been confirmed to have correlation with the ability to resistance to pathogenic challenges. The present study aimed to determine the expression profiles and levels of TLR2, 3, 4, 5, 6, 8 and 10 in the lung, trachea, intestine, stomach, liver, spleen, uterine horn and body, cervix, ovary, oviduct and hypothalamus of female rabbits, and whether the expression level of TLR2 and 4 in the ovary, oviduct, uterine horn and body, and cervix were affected by lipopolyasaccharide (LPS). The tissues of the lung, trachea, intestine, stomach, liver, spleen, uterine horn and body, cervix, ovary, oviduct and hypothalamus were collected from four rabbits which didn't be treated as 0 h. 16 rabbits in LPS group were injected with LPS (according to 0.5mg/kg body weight) and 16 rabbits in control group were injected with saline (LPS carrier), hereafter the tissues of the uterine horn and body, cervix, ovary and oviduct from 32 rabbits were collected after 1.5, 3, 6, and 12h (n=4 each group) postinjection. The expression profiles of TLRs were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR), and expression level of TLRs were examined using quantitative real-time PCR (qRT-PCR). The results shown: TLR2, 3, 4, 5, 6, 8 and 10 were expressed in lung, trachea, intestine, stomach, liver, spleen, uterine horn and body, cervix, ovary, oviduct and hypothalamus of female rabbits, but their expression level had great difference in the same organs, and each TLR has different expression level in the different organs. After LPS-stimulation, the expression of TLR2 in the uterine body and horn was significantly higher than that in control group by 3h and 12h of postinjection (P<0.05) respectively. The expression of TLR4 in ovary and uterine body was significantly higher than that in control group by 3 and 12h of postinjection (P<0.05), and the expression of TLR4 in uterine body was greater than that in control by 3h postinjection (P<0.05). The results suggested LPS upregulated the expression of TLR2 and 4 in uterine body and horn, and the expression of TLR4 in ovary.