Detection of KSHV in transbronchial biopsies in patients with Kaposi sarcoma.

In transbronchial biopsies, Kaposi sarcoma (KS) is difficult to correctly diagnose by H&E staining due to the inherent vascular nature of the lung tissue, coupled with the subtle nature of the changes in early KS. Since KS-associated herpesvirus (KSHV) has been found in all clinical forms of KS, the detection of KSHV genomic DNA sequences and/or viral products may be helpful in the diagnosis of pulmonary KS. From their files during the past 10 years, the authors identified ten HIV/AIDS patients who were positive for KS in transbronchial biopsies and four HIV/AIDS patients with KS-negative transbronchial biopsies. Immunohistochemistry with antibodies against the latency-associated nuclear antigen (LANA-1 or LNA) of KSHV was performed. Nested polymerase chain reactions (PCR) with KSHV ORF-K1 or -K9 were performed in all cases, and the KSHV sequences were detected in 9/10 (90%) KS cases. Immunohistochemical analysis was able to detect 4/10 (40%) cases. One case was negative by both PCR and immunohistochemistry. Of the five KS cases that were not diagnosed definitively ("consistent with" or "suspicious for"), two were confirmed by both immunoreactivity and PCR. One of the negative control cases was positive for KSHV by PCR but not by immunohistochemistry. The patient was thereafter found to have a clinical history of pulmonary KS at another hospital. In conclusion, in transbronchial biopsies of the lung suspicious for KS, PCR is the most sensitive technique available for clinical diagnosis of KS. Immunohistochemistry analysis might be helpful in difficult pulmonary KS cases.

Hepatocellular lymphoepithelioma-like carcinoma associated with epstein barr virus: a hitherto unrecognized entity.

OBJECTIVE: Lymphoepithelioma-like carcinoma (LELC) is an undifferentiated carcinoma with a dense lymphoid stroma. It has been reported in diverse organs and shows variable association with Epstein-Barr virus (EBV). Only a few EBV positive cases have been observed in the hepatobiliary system, all of which were considered to be cholangiocarcinomas. We report a unique case of hepatocellular LELC arising in a cirrhotic liver with EBV demonstrated in the tumor cells. METHODS AND RESULTS: A 39-year-old Hispanic female underwent an orthotopic liver transplant for end stage liver disease secondary to chronic hepatitis C. A high-grade hepatocellular carcinoma with a dense lymphocytic infiltrate was found in the explant as well as in a portal lymph node. Three months posttransplant, the patient developed numerous hepatic nodules with enlarged periaortic and portacaval lymph nodes. Biopsy of the hepatic nodules showed a recurrent hepatocellular carcinoma devoid of a dense lymphocytic infiltrate. Both the primary and recurrent tumors were positive for EBV by molecular studies. The patient eventually expired from liver failure over a 6-week period. CONCLUSION: This case represents the first report of EBV-positive hepatocellular LELC. It is particularly interesting given the precipitous clinical outcome, which was possibly related to immunosuppresive therapy.